MyavA.10100.a

Trypsin (Rv0125 ortholog)

CENTER ID: MyavA.10100.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.10100.a.B2.GE37409 soluble domain 46 371
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.5089
RefSeq: YP_884304.1
UniProt: A0A0H3A0B0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFTATQEGDG MSKSHHHRSV WWSWLVGVLT VVGLGLGLGS GVGLAPASAA PSGLALDRFA DRPLAPIDPS AMVGQVGPQV VNIDTKFGYN NAVGAGTGIV IDPNGVVLTN NHVISGATEI SAFDVGNGQT YAVDVVGYDR TQDIAVLQLR GAAGLPTATI GGEATVGEPI VALGNVGGQG GTPNAVAGKV VALNQSVSAT DTLTGAQENL GGLIQADAPI KPGDSGGPMV NGAGQVIGVD TAATDSYKMS GGQGFAIPIG RAMAVANQIR SGAGSNTVHI GPTAFLGLGV TDNNGNGARV QRVVNTGPAA AAGIAPGDVI TGVDTVPING ATSMTEVLVP HHPGDTIAVH FRSVDGGERT ANITLAEGPP A
NT Sequence
gtgtttaccg cgacgcagga gggtgacggg atgagcaaat cgcaccacca ccgctcggtc tggtggtcat ggttggtcgg tgtgctgacc gtggtcgggc tgggcctcgg tctggggtcc ggcgtggggc tggcgccggc gtccgcggca ccgtcgggcc tggcgctgga ccggttcgcc gatcgccccc tggcgccgat cgacccgtcg gccatggtcg gtcaagtggg gccgcaagtg gtcaacatcg acaccaagtt cggctacaac aacgcggtgg gcgccggtac cggcatcgtg atcgacccga acggcgtggt gctcaccaac aaccacgtca tctcgggcgc caccgaaatc agcgcgttcg acgtcggcaa cgggcagacc tacgccgtcg acgtggtcgg ctatgaccgc acccaggaca tcgccgtgct gcagctgcgc ggcgcggccg gcctgcccac cgccaccatc ggcggcgagg ccacggtggg cgagcccatc gtcgcgctcg gcaacgtcgg cggccagggc ggcaccccca acgcggtggc cggcaaggtc gtcgcgctca accagagcgt ctcggcgacc gacacgctga ccggcgcgca ggagaacctc ggcggcctga tccaggccga cgcgccgatc aagccgggcg actccggtgg cccgatggtg aacggcgccg ggcaggtgat cggcgtggac accgccgcca ccgacagcta caagatgtcc ggcgggcagg gcttcgccat cccgatcggc cgcgccatgg ccgtcgccaa ccagatccgc tccggcgccg gctccaacac cgtgcacatc gggcccaccg cattcctcgg gctgggcgtg acggacaaca acggcaacgg cgcgcgggtg cagcgggtgg tcaacaccgg cccggccgcg gccgcgggca tcgcgcccgg cgacgtcatc accggcgtcg acaccgtccc gatcaacggg gcgacgtcga tgaccgaggt gctcgtcccg caccatcccg gtgacaccat cgcggtgcac ttccggtccg tcgacggcgg cgagcgcacc gcgaacatca ccctggcgga gggtccgccg gcctga
Details for MyavA.10100.a.B2.GE37409
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 33kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 89
Validated AA Sequence
MVGQVGPQVV NIDTKFGYNN AVGAGTGIVI DPNGVVLTNN HVISGATEIS AFDVGNGQTY AVDVVGYDRT QDIAVLQLRG AAGLPTATIG GEATVGEPIV ALGNVGGQGG TPNAVAGKVV ALNQSVSATD TLTGAQENLG GLIQADAPIK PGDSGGPMVN GAGQVIGVDT AATDSYKMSG GQGFAIPIGR AMAVANQIRS GAGSNTVHIG PTAFLGLGVT DNNGNGARVQ RVVNTGPAAA AGIAPGDVIT GVDTVPINGA TSMTEVLVPH HPGDTIAVHF RSVDGGERTA NITLAEGPPA
Validated NT Sequence
tactnnctta ggccggcgga ccctccgcca gggtgatgtt cgcggtgcgc tcgccgccgt cgacggaccg gaagtgcacc gcgatggtgt caccgggatg gtgcgggacg agcacctcgg tcatcgacgt cgccccgttg atcgggacgg tgtcgacgcc ggtgatgacg tcgccgggcg cgatgcccgc ggccgcggcc gggccggtgt tgaccacccg ctgcacccgc gcgccgttgc cgttgttgtc cgtcacgccc agcccgagga atgcggtggg cccgatgtgc acggtgttgg agccggcgcc ggagcggatc tggttggcga cggccatggc gcggccgatc gggatggcga agccctgccc gccggacatc ttgtagctgt cggtggcggc ggtgtccacg ccgatcacct gcccggcgcc gttcaccatc gggccaccgg agtcgcccgg cttgatcggc gcgtcggcct ggatcaggcc gccgaggttc tcctgcgcgc cggtcagcgt gtcggtcgcc gagacgctct ggttgagcgc gacgaccttg ccggccaccg cgttgggggt gccgccctgg ccgccgacgt tgccgagcgc gacgatgggc tcgcccaccg tggcctcgcc gccgatggtg gcggtgggca ggccggccgc gccgcgcagc tgcagcacgg cgatgtcctg ggtgcggtca tagccgacca cgtcgacggc gtaggtctgc ccgttgccga cgtcgaacgc gctgatttcg gtggcgcccg agatgacgtg gttgttggtg agcaccacgc cgttcgggtc gatcacgatg ccggtaccgg cgcccaccgc gttgttgtag ccgaacttgg tgtcgatgtt gaccacttgc ggccccactt gaccgaccat ggccgacggg tcgatcggcg ccagggggcg atcggcgaac cggtccagcg ccaggcccga cggtgccgcg gacgccggc
Expected Protein Sequence
MAHHHHHHPA SAAPSGLALD RFADRPLAPI DPSAMVGQVG PQVVNIDTKF GYNNAVGAGT GIVIDPNGVV LTNNHVISGA TEISAFDVGN GQTYAVDVVG YDRTQDIAVL QLRGAAGLPT ATIGGEATVG EPIVALGNVG GQGGTPNAVA GKVVALNQSV SATDTLTGAQ ENLGGLIQAD APIKPGDSGG PMVNGAGQVI GVDTAATDSY KMSGGQGFAI PIGRAMAVAN QIRSGAGSNT VHIGPTAFLG LGVTDNNGNG ARVQRVVNTG PAAAAGIAPG DVITGVDTVP INGATSMTEV LVPHHPGDTI AVHFRSVDGG ERTANITLAE GPPA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catccggcgt ccgcggcacc gtcgggcctg gcgctggacc ggttcgccga tcgccccctg gcgccgatcg acccgtcggc catggtcggt caagtggggc cgcaagtggt caacatcgac accaagttcg gctacaacaa cgcggtgggc gccggtaccg gcatcgtgat cgacccgaac ggcgtggtgc tcaccaacaa ccacgtcatc tcgggcgcca ccgaaatcag cgcgttcgac gtcggcaacg ggcagaccta cgccgtcgac gtggtcggct atgaccgcac ccaggacatc gccgtgctgc agctgcgcgg cgcggccggc ctgcccaccg ccaccatcgg cggcgaggcc acggtgggcg agcccatcgt cgcgctcggc aacgtcggcg gccagggcgg cacccccaac gcggtggccg gcaaggtcgt cgcgctcaac cagagcgtct cggcgaccga cacgctgacc ggcgcgcagg agaacctcgg cggcctgatc caggccgacg cgccgatcaa gccgggcgac tccggtggcc cgatggtgaa cggcgccggg caggtgatcg gcgtggacac cgccgccacc gacagctaca agatgtccgg cgggcagggc ttcgccatcc cgatcggccg cgccatggcc gtcgccaacc agatccgctc cggcgccggc tccaacaccg tgcacatcgg gcccaccgca ttcctcgggc tgggcgtgac ggacaacaac ggcaacggcg cgcgggtgca gcgggtggtc aacaccggcc cggccgcggc cgcgggcatc gcgcccggcg acgtcatcac cggcgtcgac accgtcccga tcaacggggc gacgtcgatg accgaggtgc tcgtcccgca ccatcccggt gacaccatcg cggtgcactt ccggtccgtc gacggcggcg agcgcaccgc gaacatcacc ctggcggagg gtccgccggc ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat