SSGCID: Target Page

MyavA.01613.n

Acyl-CoA dehydrogenase domain protein

CENTER ID:	MyavA.01613.n
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01613.n.A1.GE29155	Full length( MyavA.01613.n )		1	390

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.2557
RefSeq:	YP_881810.1
UniProt:	A0A0H2ZS40

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDLDFTPDQL EFRDQVRTWL DENKPAEPRP RDDAGIREYD LAWQRTQAEG GWAGIAWPTE YGGKGLTLLQ QLIWYEEYAA RGFPGIDACF VGNSHAGPTL ITRATDEQKS FHLPKILGGE VIWCQGFSEP DAGSDLAALR TKAVVDGEDL VVSGQKLWTS FATVADYQEL LVRTDNTGSK HKGITWVICD MSTPGIDVRP IETIEGGSEF CEVFYDDVRI PLSNVVGDVG EGWSVAMATL SFERGTAFTA NQVRLAKIIE DLIDYARDHV GPDGRRPAIA DDDIARRLAR ARASVASLRA LTYTNICEAM KTETPGPRGS IVKLMYAELA KEIGRLAMDV VGPASVRYSS RWDVDGWVGY YYYSFSQAIG GGTSEIQRNI VGERVLGLPR

NT Sequence

ATGGATCTCG ACTTCACGCC TGACCAGCTC GAGTTCCGTG ACCAGGTCCG CACATGGCTC GACGAGAACA AACCGGCTGA ACCCCGCCCG CGCGACGACG CGGGGATCCG TGAATACGAC CTTGCCTGGC AACGCACTCA GGCCGAGGGC GGCTGGGCCG GCATCGCCTG GCCCACCGAA TACGGCGGCA AGGGGCTGAC GCTGCTTCAG CAGTTGATCT GGTACGAGGA GTACGCCGCG CGGGGGTTCC CCGGCATCGA TGCATGCTTC GTCGGCAACT CGCACGCCGG GCCCACTCTG ATCACCAGGG CCACCGACGA ACAGAAGTCT TTCCACTTGC CGAAAATCCT TGGTGGTGAA GTGATTTGGT GTCAGGGCTT CTCCGAGCCC GACGCCGGAT CGGACCTGGC GGCATTGCGC ACCAAGGCGG TGGTGGACGG CGAGGACCTG GTGGTCTCGG GGCAGAAGCT GTGGACCAGC TTCGCCACCG TCGCCGATTA CCAGGAGCTG CTGGTGCGCA CGGACAACAC CGGAAGCAAG CACAAGGGCA TCACCTGGGT GATCTGCGAC ATGAGCACGC CGGGCATCGA CGTCCGCCCT ATCGAGACCA TCGAGGGCGG CTCGGAATTC TGCGAAGTGT TTTACGACGA TGTCCGAATC CCGCTGAGCA ACGTGGTCGG CGATGTCGGC GAAGGCTGGT CGGTTGCGAT GGCCACCCTG TCCTTCGAGC GCGGCACCGC GTTCACCGCC AACCAGGTGC GGCTGGCGAA GATCATCGAG GACCTCATCG ACTATGCCCG CGACCATGTC GGGCCGGACG GCCGCAGGCC CGCCATCGCC GACGACGACA TCGCGCGACG GCTGGCGCGG GCCCGCGCCT CGGTGGCGTC GCTGCGGGCG CTCACCTACA CCAATATCTG CGAGGCCATG AAGACCGAGA CGCCCGGCCC GCGGGGTTCG ATCGTCAAGC TGATGTATGC CGAATTGGCC AAGGAGATCG GCAGATTGGC GATGGATGTT GTCGGTCCCG CCTCGGTCCG GTATTCATCG CGCTGGGACG TCGACGGCTG GGTCGGCTAC TACTACTACA GCTTCTCCCA AGCCATCGGC GGCGGGACGT CGGAGATTCA GCGCAACATC GTCGGCGAAC GAGTGTTGGG GCTGCCGCGA

Details for MyavA.01613.n.A1.GE29155

HARVESTED ON:	4/27/2010
SEQUENCED ON:	5/1/2010
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	55

Validated AA Sequence

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggatc tcgacttcac gcctgaccag ctcgagttcc gtgaccaggt ccgcacatgg ctcgacgaga acaaaccggc tgaaccccgc ccgcgcgacg acgcggggat ccgtgaatac gaccttgcct ggcaacgcac tcaggccgag ggcggctggg ccggcatcgc ctggcccacc gaatacggcg gcaaggggct gacgctgctt cagcagttga tctggtacga ggagtacgcc gcgcgggggt tccccggcat cgatgcatgc ttcgtcggca actcgcacgc cgggcccact ctgatcacca gggccaccga cgaacagaag tctttccact tgccgaaaat ccttggtggt gaagtgattt ggtgtcaggg cttctccgag cccgacgccg gatcggacct ggcggcattg cgcaccaagg cggtggtgga cggcgaggac ctggtggtct cggggcagaa gctgtggacc agcttcgcca ccgtcgccga ttaccaggag ctgctggtgc gcacggacaa caccggaagc aagcacaagg gcatcacctg ggtgatctgc gacatgagca cgccgggcat cgacgtccgc cctatcgaga ccatcgaggg cggctcgga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDLDFTPDQ LEFRDQVRTW LDENKPAEPR PRDDAGIREY DLAWQRTQAE GGWAGIAWPT EYGGKGLTLL QQLIWYEEYA ARGFPGIDAC FVGNSHAGPT LITRATDEQK SFHLPKILGG EVIWCQGFSE PDAGSDLAAL RTKAVVDGED LVVSGQKLWT SFATVADYQE LLVRTDNTGS KHKGITWVIC DMSTPGIDVR PIETIEGGSE FCEVFYDDVR IPLSNVVGDV GEGWSVAMAT LSFERGTAFT ANQVRLAKII EDLIDYARDH VGPDGRRPAI ADDDIARRLA RARASVASLR ALTYTNICEA MKTETPGPRG SIVKLMYAEL AKEIGRLAMD VVGPASVRYS SRWDVDGWVG YYYYSFSQAI GGGTSEIQRN IVGERVLGLP R

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatctcgac ttcacgcctg accagctcga gttccgtgac caggtccgca catggctcga cgagaacaaa ccggctgaac cccgcccgcg cgacgacgcg gggatccgtg aatacgacct tgcctggcaa cgcactcagg ccgagggcgg ctgggccggc atcgcctggc ccaccgaata cggcggcaag gggctgacgc tgcttcagca gttgatctgg tacgaggagt acgccgcgcg ggggttcccc ggcatcgatg catgcttcgt cggcaactcg cacgccgggc ccactctgat caccagggcc accgacgaac agaagtcttt ccacttgccg aaaatccttg gtggtgaagt gatttggtgt cagggcttct ccgagcccga cgccggatcg gacctggcgg cattgcgcac caaggcggtg gtggacggcg aggacctggt ggtctcgggg cagaagctgt ggaccagctt cgccaccgtc gccgattacc aggagctgct ggtgcgcacg gacaacaccg gaagcaagca caagggcatc acctgggtga tctgcgacat gagcacgccg ggcatcgacg tccgccctat cgagaccatc gagggcggct cggaattctg cgaagtgttt tacgacgatg tccgaatccc gctgagcaac gtggtcggcg atgtcggcga aggctggtcg gttgcgatgg ccaccctgtc cttcgagcgc ggcaccgcgt tcaccgccaa ccaggtgcgg ctggcgaaga tcatcgagga cctcatcgac tatgcccgcg accatgtcgg gccggacggc cgcaggcccg ccatcgccga cgacgacatc gcgcgacggc tggcgcgggc ccgcgcctcg gtggcgtcgc tgcgggcgct cacctacacc aatatctgcg aggccatgaa gaccgagacg cccggcccgc ggggttcgat cgtcaagctg atgtatgccg aattggccaa ggagatcggc agattggcga tggatgttgt cggtcccgcc tcggtccggt attcatcgcg ctgggacgtc gacggctggg tcggctacta ctactacagc ttctcccaag ccatcggcgg cgggacgtcg gagattcagc gcaacatcgt cggcgaacga gtgttggggc tgccgcgaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa