MyavA.01613.m

Acyl-CoA dehydrogenase

CENTER ID: MyavA.01613.m
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01613.m.A1.GE29154 Full length( MyavA.01613.m ) 1 396
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.2531
RefSeq: YP_881785.1
UniProt: A0A0H3A223

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEYGMGPELE AFRAEVRAFI AEHAPPIPPR AGVRSAENEA ELKALQDWTA RLFEAGYVGA DWPVEFGGRD DRSAEHAIVV GEELARAAVP GVPSGNALAS HALIHYGTDD QRRRHLPEIR AGRQLWCQLF SEPGAGSDLA SLRTRAVLDG DTYTVNGQKV WTTDGHWADY GYLLARTDSD APKHKGISAF ILDMSSPGIT VRPLRELTGT SDFNEVFFDS VEVPAEAMIG EPGQGWAIAN ATLGHERTSV GAAVVKLRLA IEALVQLASH VTVDGRLAID NDRIRDRIGE FSAEVEALSA LTYANLTRWL RGTERMHDGA MAKLMFSELN LEMARFAVEL GGEAGVLVEG DPDVLDGGRW QDEWLYARAY TIAGGSSEIM RNLIAERGLA LPRDRR
NT Sequence
GTGGAATACG GAATGGGTCC CGAACTGGAG GCCTTTCGCG CGGAGGTGCG GGCTTTCATT GCGGAGCATG CACCGCCCAT ACCGCCGCGG GCGGGCGTGC GCAGCGCTGA GAATGAAGCC GAACTCAAGG CGCTTCAGGA TTGGACGGCG CGTCTGTTCG AAGCCGGCTA CGTCGGCGCC GACTGGCCCG TGGAGTTCGG CGGCCGCGAT GACCGCTCCG CTGAACACGC CATCGTGGTG GGCGAGGAGC TGGCCCGGGC CGCGGTGCCG GGCGTGCCGA GCGGTAACGC GCTGGCATCG CATGCGCTGA TCCACTACGG CACCGATGAC CAGCGCCGCA GGCATCTGCC CGAGATCCGC GCGGGTCGAC AGCTGTGGTG CCAATTGTTC AGCGAGCCCG GTGCAGGAAG CGATCTGGCG TCGCTGCGTA CCCGCGCTGT TCTCGACGGT GATACCTATA CCGTCAACGG GCAGAAGGTG TGGACCACCG ACGGACACTG GGCCGACTAC GGATATCTGT TGGCGCGCAC CGATTCCGAC GCCCCCAAGC ACAAGGGCAT CAGTGCGTTT ATCCTCGACA TGTCCAGCCC GGGCATCACG GTGCGCCCGC TGCGCGAGCT GACCGGCACC TCGGACTTCA ACGAGGTGTT CTTCGATTCC GTCGAGGTGC CGGCCGAGGC GATGATCGGA GAACCGGGAC AGGGCTGGGC GATCGCAAAC GCCACGTTGG GGCATGAACG TACCAGCGTG GGCGCCGCGG TCGTCAAGTT GAGGCTGGCC ATTGAGGCAT TGGTGCAACT CGCCTCGCAC GTCACCGTCG ACGGTCGCCT CGCGATCGAC AACGACCGTA TTCGTGATCG GATCGGCGAG TTCAGCGCCG AGGTGGAGGC GCTTTCCGCG CTGACGTACG CCAATCTCAC GCGTTGGCTG CGTGGCACCG AGCGCATGCA CGACGGCGCG ATGGCCAAGC TGATGTTCAG CGAGCTGAAT CTCGAGATGG CACGCTTCGC AGTCGAACTC GGCGGCGAGG CAGGCGTTTT GGTGGAGGGC GACCCGGACG TTCTCGACGG CGGTCGCTGG CAGGACGAGT GGTTGTACGC TCGGGCCTAC ACCATCGCCG GCGGCAGTTC GGAGATCATG CGCAACCTGA TCGCCGAGCG GGGCTTGGCG CTGCCGCGGG ATCGGCGC
Details for MyavA.01613.m.A1.GE29154
HARVESTED ON: 4/27/2010
SEQUENCED ON: 5/1/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MVEYGMGPEL EAFRAEVRAF IAEHAPPIPP RAGVRSAENE AELKALQDWT ARLFEAGYVG ADWPVEFGGR DDRSAEHAIV VGEELARAAV PGVPSGNALA SHALIHYGTD DQRRRHLPEI RAGRQLWCQL FSEPGAGSDL ASLRTRAVLD GDTYTVNGQK VWTTDGHWAD YGYLLARTDS DAPKHKGISA FILDMSSPGI TVRPLRELTG TSDFNEVFFD SVEVPAEAMI GEPGQGWAIA NATLGHERTS VGAAVVKLRL AIEALVQLAS HVTVDGRLAI DNDRIRDRIG EFSAEVEALS ALTYANLTRW LRGTERMHDG AMAKLMFSEL NLEMARFAVE LGGEAGVLVE GDPDVLDGGR WQDEWLYARA YTIAGGSSEI MRNLIAERGL ALPRDRR
Validated NT Sequence
ggtaccctgg aagctcagac ccagggtcct ggttcgatgg tggaatacgg aatgggtccc gaactggagg cctttcgcgc ggaggtgcgg gctttcattg cggagcatgc accgcccata ccgccgcggg cgggcgtgcg cagcgctgag aatgaagccg aactcaaggc gcttcaggat tggacggcgc gtctgttcga agccggctac gtcggcgccg actggcccgt ggagttcggc ggccgcgatg accgctccgc tgaacacgcc atcgtggtgg gcgaggagct ggcccgggcc gcggtgccgg gcgtgccgag cggtaacgcg ctggcatcgc atgcgctgat ccactacggc accgatgacc agcgccgcag gcatctgccc gagatccgcg cgggtcgaca gctgtggtgc caattgttca gcgagcccgg tgcaggaagc gatctggcgt cgctgcgtac ccgcgctgtt ctcgacggtg atacctatac cgtcaacggg cagaaggtgt ggaccaccga cggacactgg gccgactacg gatatctgtt ggcgcgcacc gattccgacg cccccaagca caagggcatc agtgcgttta tcctcgacat gtccagcccg ggcatcacgg tgcgcccgct gcgcgagctg accggcacct cggacttcaa cgaggtgttc ttcgattccg tcgaggtgcc ggccgaggcg atgatcggag aaccgggaca gggctgggcg atcgcaaacg ccacgttggg gcatgaacgt accagcgtgg gcgccgcggt cgtcaagttg aggctggcca ttgaggcatt ggtgcaactc gcctcgcacg tcaccgtcga cggtcgcctc gcgatcgaca acgaccgtat tcgtgatcgg atcggcgagt tcagcgccga ggtggaggcg ctttccgcgc tgacgtacgc caatctcacg cgttggctgc gtggcaccga gcgcatgcac gacggcgcga tggccaagct gatgttcagc gagctgaatc tcgagatggc acgcttcgca gtcgaactcg gcggcgaggc aggcgttttg gtggagggcg acccggacgt tctcgacggc ggtcgctggc aggacgagtg gttgtacgct cgggcctaca ccatcgccgg cggcagttcg gagatcatgc gcaacctgat cgccgagcgg ggcttggcgc tgccgcggga tcggcgctaa ta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVEYGMGPE LEAFRAEVRA FIAEHAPPIP PRAGVRSAEN EAELKALQDW TARLFEAGYV GADWPVEFGG RDDRSAEHAI VVGEELARAA VPGVPSGNAL ASHALIHYGT DDQRRRHLPE IRAGRQLWCQ LFSEPGAGSD LASLRTRAVL DGDTYTVNGQ KVWTTDGHWA DYGYLLARTD SDAPKHKGIS AFILDMSSPG ITVRPLRELT GTSDFNEVFF DSVEVPAEAM IGEPGQGWAI ANATLGHERT SVGAAVVKLR LAIEALVQLA SHVTVDGRLA IDNDRIRDRI GEFSAEVEAL SALTYANLTR WLRGTERMHD GAMAKLMFSE LNLEMARFAV ELGGEAGVLV EGDPDVLDGG RWQDEWLYAR AYTIAGGSSE IMRNLIAERG LALPRDRR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggaatac ggaatgggtc ccgaactgga ggcctttcgc gcggaggtgc gggctttcat tgcggagcat gcaccgccca taccgccgcg ggcgggcgtg cgcagcgctg agaatgaagc cgaactcaag gcgcttcagg attggacggc gcgtctgttc gaagccggct acgtcggcgc cgactggccc gtggagttcg gcggccgcga tgaccgctcc gctgaacacg ccatcgtggt gggcgaggag ctggcccggg ccgcggtgcc gggcgtgccg agcggtaacg cgctggcatc gcatgcgctg atccactacg gcaccgatga ccagcgccgc aggcatctgc ccgagatccg cgcgggtcga cagctgtggt gccaattgtt cagcgagccc ggtgcaggaa gcgatctggc gtcgctgcgt acccgcgctg ttctcgacgg tgatacctat accgtcaacg ggcagaaggt gtggaccacc gacggacact gggccgacta cggatatctg ttggcgcgca ccgattccga cgcccccaag cacaagggca tcagtgcgtt tatcctcgac atgtccagcc cgggcatcac ggtgcgcccg ctgcgcgagc tgaccggcac ctcggacttc aacgaggtgt tcttcgattc cgtcgaggtg ccggccgagg cgatgatcgg agaaccggga cagggctggg cgatcgcaaa cgccacgttg gggcatgaac gtaccagcgt gggcgccgcg gtcgtcaagt tgaggctggc cattgaggca ttggtgcaac tcgcctcgca cgtcaccgtc gacggtcgcc tcgcgatcga caacgaccgt attcgtgatc ggatcggcga gttcagcgcc gaggtggagg cgctttccgc gctgacgtac gccaatctca cgcgttggct gcgtggcacc gagcgcatgc acgacggcgc gatggccaag ctgatgttca gcgagctgaa tctcgagatg gcacgcttcg cagtcgaact cggcggcgag gcaggcgttt tggtggaggg cgacccggac gttctcgacg gcggtcgctg gcaggacgag tggttgtacg ctcgggccta caccatcgcc ggcggcagtt cggagatcat gcgcaacctg atcgccgagc ggggcttggc gctgccgcgg gatcggcgca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a