MyavA.01613.k

Acyl-CoA dehydrogenase, middle domain protein

CENTER ID: MyavA.01613.k
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01613.k.A1.GE29152 Full length( MyavA.01613.k ) 1 415
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.2440
RefSeq: YP_881692.1
UniProt: A0A0H2ZRP1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSESVEEFR GRARAWLAAN MPRLDPARAA LLQRDELSSW HRARELQKLL YDGGFAGICF PREYGGLGLS YEYKKAFDAE SVGYETPLLL NVPSFAICCA TILDMGSEQQ KRTHIAAALR GEEVLVQLLS EPSGGSDLAG VLTRAERRGD RWIINGAKTW STWAFAADYG LCLARTDWQV PKHEGLTMFL VPLAHPGVTI NRIRQVNGSV EFCEEFFDDV DVGDDAVLGE PGKGWDVASR QLYHERRTMG DGSEYTSGPG IAEAQDVSVD LLSLIDAVGG PGDPRLQEAV GRALVHRAVH GQLSEHVFHA VVDGTLPPAA GSIIRLSMAE VHNVETDTAL AVAGPAAVVD GAGGLLDIGT RYLGRQIASI GGGTTEMARN VIGERVLGFL RERADDRGVP FNQVRRGRNP GGQRN
NT Sequence
GTGACGTCGG AGTCGGTCGA GGAATTTCGG GGCCGGGCCA GGGCCTGGCT GGCCGCGAAC ATGCCGCGAC TCGATCCCGC CCGGGCGGCG CTGCTGCAGC GCGACGAGCT CAGCTCCTGG CACCGGGCCC GGGAATTGCA AAAGCTGCTC TACGACGGCG GATTCGCCGG AATCTGCTTC CCGCGCGAAT ACGGCGGGCT CGGGCTGAGC TACGAATACA AGAAGGCGTT CGACGCCGAG TCGGTGGGTT ACGAGACGCC GCTGCTGCTC AACGTCCCGT CCTTCGCCAT CTGCTGCGCC ACGATCCTGG ACATGGGCAG CGAGCAGCAG AAGCGCACCC ACATCGCGGC CGCGCTGCGC GGCGAGGAAG TGCTGGTGCA GTTGCTGTCC GAGCCCAGCG GGGGATCGGA CCTGGCCGGT GTGCTCACCC GGGCCGAGCG CCGCGGGGAT CGCTGGATCA TCAACGGCGC CAAGACCTGG AGCACGTGGG CGTTCGCGGC GGACTACGGC CTGTGCCTGG CCCGCACCGA CTGGCAGGTG CCCAAACACG AAGGCCTGAC CATGTTTCTG GTGCCGCTTG CCCATCCCGG CGTCACCATC AATCGCATCC GGCAGGTCAA CGGCTCGGTC GAGTTCTGCG AAGAGTTCTT CGACGACGTC GACGTGGGCG ACGACGCCGT GCTGGGTGAA CCGGGGAAAG GATGGGACGT CGCGTCGCGG CAGCTCTATC ACGAGCGCCG CACGATGGGA GACGGCTCGG AGTACACCAG CGGCCCGGGA ATCGCTGAGG CCCAAGACGT TTCGGTCGAT CTGCTGTCGT TGATCGACGC CGTCGGCGGT CCCGGCGACC CGCGGCTGCA GGAAGCGGTG GGTCGGGCGC TGGTGCACCG GGCCGTGCAC GGACAGCTCA GCGAGCACGT CTTTCACGCC GTGGTCGACG GAACCCTGCC GCCGGCCGCG GGATCGATCA TCCGCTTGTC CATGGCCGAG GTGCACAACG TGGAAACCGA CACCGCGCTG GCGGTGGCCG GCCCGGCGGC CGTGGTCGAC GGCGCCGGTG GCCTGCTCGA CATCGGGACG CGCTATCTGG GTCGTCAGAT CGCCAGCATC GGCGGCGGCA CCACCGAGAT GGCGCGCAAC GTGATCGGCG AGCGGGTGCT CGGCTTCCTG CGCGAGCGCG CCGACGACCG CGGCGTCCCG TTCAACCAGG TGCGGCGCGG CAGAAACCCA GGAGGCCAAC GCAAT
Details for MyavA.01613.k.A1.GE29152
HARVESTED ON: 4/27/2010
SEQUENCED ON: 5/1/2010
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MVTSESVEEF RGRARAWLAA NMPRLDPARA ALLQRDELSS WHRARELQKL LYDGGFAGIC FPREYGGLGL SYEYKKAFDA ESVGYETPLL LNVPSFAICC ATILDMGSEQ QKRTHIAAAL RGEEVLVQLL SEPSGGSDLA GVLTRAERRG DRWIINGAKT WSTWAFAADY GLCLARTDWQ VPKHEGLTMF LVPLAHPGVT INRIRQVNGS VEFCEEFFDD VDVGDDAVLG EPGKGWDVAS RQLYHERRTM GDGSEYTSGP GIAEAQDVSV DLLSLIDAVG GPGDPRLQEA VGRALVHRAV HGQLSEHVFH AVVDGTLPPA AGSIIRLSMA EVHNVETDTA LAVAGPAAVV DGAGGLLDIG TRYLGRQIAS IGGGTTEMAR NVIGERVLGF LRERADDRGV PFNQVRRGRN PGGQR
Validated NT Sequence
accctggaag ctcagaccca gggtcctggt tcgatggtga cgtcggagtc ggtcgaggaa tttcggggcc gggccagggc ctggctggcc gcgaacatgc cgcgactcga tcccgcccgg gcggcgctgc tgcagcgcga cgagctcagc tcctggcacc gggcccggga attgcaaaag ctgctctacg acggcggatt cgccggaatc tgcttcccgc gcgaatacgg cgggctcggg ctgagctacg aatacaagaa ggcgttcgac gccgagtcgg tgggttacga gacgccgctg ctgctcaacg tcccgtcctt cgccatctgc tgcgccacga tcctggacat gggcagcgag cagcagaagc gcacccacat cgcggccgcg ctgcgcggcg aggaagtgct ggtgcagttg ctgtccgagc ccagcggggg atcggacctg gccggtgtgc tcacccgggc cgagcgccgc ggggatcgct ggatcatcaa cggcgccaag acctggagca cgtgggcgtt cgcggcggac tacggcctgt gcctggcccg caccgactgg caggtgccca aacacgaagg cctgaccatg tttctggtgc cgcttgccca tcccggcgtc accatcaatc gcatccggca ggtcaacggc tcggtcgagt tctgcgaaga gttcttcgac gacgtcgacg tgggcgacga cgccgtgctg ggtgaaccgg ggaaaggatg ggacgtcgcg tcgcggcagc tctatcacga gcgccgcacg atgggagacg gctcggagta caccagcggc ccgggaatcg ctgaggccca agacgtttcg gtcgatctgc tgtcgttgat cgacgccgtc ggcggtcccg gcgacccgcg gctgcaggaa gcggtgggtc gggcgctggt gcaccgggcc gtgcacggac agctcagcga gcacgtcttt cacgccgtgg tcgacggaac cctgccgccg gccgcgggat cgatcatccg cttgtccatg gccgaggtgc acaacgtgga aaccgacacc gcgctggcgg tggccggccc ggcggccgtg gtcgacggcg ccggtggcct gctcgacatc gggacgcgct atctgggtcg tcagatcgcc agcatcggcg gcggcaccac cgagatggcg cgcaacgtga tcggcgagcg ggtgctcggc ttcctgcgcg agcgcgccga cgaccgcggc gtcccgttca accaggtgcg gcgcggcaga aacccaggag gccaacgcaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSESVEE FRGRARAWLA ANMPRLDPAR AALLQRDELS SWHRARELQK LLYDGGFAGI CFPREYGGLG LSYEYKKAFD AESVGYETPL LLNVPSFAIC CATILDMGSE QQKRTHIAAA LRGEEVLVQL LSEPSGGSDL AGVLTRAERR GDRWIINGAK TWSTWAFAAD YGLCLARTDW QVPKHEGLTM FLVPLAHPGV TINRIRQVNG SVEFCEEFFD DVDVGDDAVL GEPGKGWDVA SRQLYHERRT MGDGSEYTSG PGIAEAQDVS VDLLSLIDAV GGPGDPRLQE AVGRALVHRA VHGQLSEHVF HAVVDGTLPP AAGSIIRLSM AEVHNVETDT ALAVAGPAAV VDGAGGLLDI GTRYLGRQIA SIGGGTTEMA RNVIGERVLG FLRERADDRG VPFNQVRRGR NPGGQRN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacgtcg gagtcggtcg aggaatttcg gggccgggcc agggcctggc tggccgcgaa catgccgcga ctcgatcccg cccgggcggc gctgctgcag cgcgacgagc tcagctcctg gcaccgggcc cgggaattgc aaaagctgct ctacgacggc ggattcgccg gaatctgctt cccgcgcgaa tacggcgggc tcgggctgag ctacgaatac aagaaggcgt tcgacgccga gtcggtgggt tacgagacgc cgctgctgct caacgtcccg tccttcgcca tctgctgcgc cacgatcctg gacatgggca gcgagcagca gaagcgcacc cacatcgcgg ccgcgctgcg cggcgaggaa gtgctggtgc agttgctgtc cgagcccagc gggggatcgg acctggccgg tgtgctcacc cgggccgagc gccgcgggga tcgctggatc atcaacggcg ccaagacctg gagcacgtgg gcgttcgcgg cggactacgg cctgtgcctg gcccgcaccg actggcaggt gcccaaacac gaaggcctga ccatgtttct ggtgccgctt gcccatcccg gcgtcaccat caatcgcatc cggcaggtca acggctcggt cgagttctgc gaagagttct tcgacgacgt cgacgtgggc gacgacgccg tgctgggtga accggggaaa ggatgggacg tcgcgtcgcg gcagctctat cacgagcgcc gcacgatggg agacggctcg gagtacacca gcggcccggg aatcgctgag gcccaagacg tttcggtcga tctgctgtcg ttgatcgacg ccgtcggcgg tcccggcgac ccgcggctgc aggaagcggt gggtcgggcg ctggtgcacc gggccgtgca cggacagctc agcgagcacg tctttcacgc cgtggtcgac ggaaccctgc cgccggccgc gggatcgatc atccgcttgt ccatggccga ggtgcacaac gtggaaaccg acaccgcgct ggcggtggcc ggcccggcgg ccgtggtcga cggcgccggt ggcctgctcg acatcgggac gcgctatctg ggtcgtcaga tcgccagcat cggcggcggc accaccgaga tggcgcgcaa cgtgatcggc gagcgggtgc tcggcttcct gcgcgagcgc gccgacgacc gcggcgtccc gttcaaccag gtgcggcgcg gcagaaaccc aggaggccaa cgcaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa