SSGCID: Target Page

MyavA.01613.i

Acyl-CoA dehydrogenase family protein

CENTER ID:	MyavA.01613.i
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01613.i.A1.GE29150	Full length( MyavA.01613.i )		1	394

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.1837
RefSeq:	YP_881098.1
UniProt:	A0A0H3A0P8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEYGMRPELA EFRAEVRAFV AEHAPAIPPR AGVRSAENDA EFKALQEWTG RLFEAGYVGA DWPVEFGGRG DFTPEHSIIV GEELARAQAP SAQSGSVLAS HALIHYGTDE QRRRHLPEIR GGRELWCQLF SEPGAGSDLA SLRTRAVRHG DTYTIDGQKV WTTDGHWARY GYLLARTDPD APKHKGISAF ILDMSAPGVS VRPLRELTGT SDFNEVFFDG VEVPADAMIG APGQGWAIAN ATLAHERTNV GGIVVKLRLA IDALIDLARR VTIDGGPAID SDRVRDRIGQ FSAEVEALSA LTYANLTRWL RGRERMHDAA MGKLMFSEIN LEMAAFAVEL AGEAGVLVEG DDNVLDGGRW QDEWLYARAY TIAGGSSEIM RNLIAERGLG LPRD

NT Sequence

ATGGAATACG GCATGAGACC GGAGTTGGCG GAGTTCCGCG CCGAGGTGCG GGCGTTCGTC GCCGAGCACG CCCCGGCGAT TCCGCCCCGG GCGGGGGTTC GCAGCGCTGA GAACGACGCC GAGTTCAAGG CGTTGCAGGA GTGGACCGGA CGCCTGTTCG AGGCGGGCTA CGTGGGAGCG GACTGGCCTG TGGAGTTCGG GGGTCGTGGT GACTTCACAC CCGAACACTC GATCATCGTG GGGGAGGAGC TTGCTCGCGC GCAGGCACCC AGCGCGCAGA GCGGCAGCGT GCTCGCGTCG CACGCGCTGA TCCATTACGG CACAGACGAA CAGCGCCGGC GACATCTCCC CGAGATCCGC GGGGGCCGTG AACTGTGGTG CCAGCTGTTC AGCGAGCCGG GTGCGGGTAG TGACCTGGCG TCGCTACGCA CCCGCGCCGT ACGCCACGGC GACACCTACA CGATCGATGG CCAGAAGGTG TGGACCACCG ACGGGCACTG GGCGCGATAC GGCTATCTGC TGGCACGCAC CGACCCCGAC GCACCGAAAC ACAAGGGCAT CAGCGCATTC ATCCTCGACA TGTCCGCACC GGGCGTCAGT GTGCGACCGC TACGTGAGCT GACGGGTACC TCCGATTTCA ACGAGGTGTT CTTCGACGGT GTCGAGGTGC CGGCCGACGC GATGATCGGC GCACCCGGCC AGGGTTGGGC GATCGCGAAC GCCACGCTCG CCCATGAGCG CACCAATGTC GGTGGGATCG TGGTGAAGCT GCGGCTCGCC ATTGATGCTT TGATCGATCT GGCCCGTCGT GTCACGATCG ACGGCGGGCC GGCGATCGAC AGCGACCGGG TCCGCGACCG GATCGGTCAG TTCAGCGCCG AGGTGGAGGC GTTGTCGGCG TTGACATATG CCAACCTGAC CCGCTGGCTT CGCGGCCGCG AACGCATGCA CGACGCGGCG ATGGGCAAGC TGATGTTCAG CGAAATCAAC TTGGAGATGG CGGCATTCGC GGTCGAGTTG GCCGGCGAGG CGGGCGTGCT CGTCGAAGGA GACGACAATG TTCTCGACGG TGGCCGCTGG CAGGACGAGT GGCTGTACGC CCGCGCCTAC ACCATCGCGG GCGGCAGTTC GGAAATCATG CGCAACCTCA TCGCCGAGCG GGGACTTGGC CTACCCCGCG AT

Details for MyavA.01613.i.A1.GE29150

HARVESTED ON:	4/27/2010
SEQUENCED ON:	5/1/2010
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

ccctggaagc tcagacccag ggtcctggtt cgatggaata cggcatgaga ccggagttgg cggagttccg cgccgaggtg cgggcgttcg tcgccgagca cgccccggcg attccgcccc gggcgggggt tcgcagcgct gagaacgacg ccgagttcaa ggcgttgcag gagtggaccg gacgcctgtt cgaggcgggc tacgtgggag cggactggcc tgtggagttc gggggtcgtg gtgacttcac acccgaacac tcgatcatcg tgggggagga gcttgctcgc gcgcaggcac ccagcgcgca gagcggcagc gtgctcgcgt cgcacgcgct gatccattac ggcacagacg aacagcgccg gcgacatctc cccgagatcc gcgggggccg tgaactgtgg tgccagctgt tcagcgagcc gggtgcgggt agtgacctgg cgtcgctacg cacccgcgcc gtacgccacg gcgacaccta cacgatcgat ggccagaagg tgtggaccac cgacgggcac tgggcgcgat acggctatct gctggcacgc accgaccccg acgcaccgaa acacaagggc atcagcgcat tcatcctcga catgtccgca ccgggcgtca gtgtgcgacc gctacgtgag ctgacgggta cctccgattt caacgaggtg ttcttcgacg gtgtcgaggt gccggccgac gcgatgatcg gcgcacccgg ccagggttgg gcgatcgcga acgccacgct cgcccatgag cgcaccaatg tcggtgggat cgtggtgaag ctgcggctcg ccattgatgc tttgatcgat ctggcccgtc gtgtcacgat cgacggcggg ccggcgatcg acagcgaccg ggtccgcgac cggatcggtc agttcagcgc cgaggtggag gcgttgtcgg cgttgacata tgccaacctg acccgctggc ttcgcggccg cgaacgcatg cacgacgcgg cgatgggcaa gctgatgttc agcgaaatca acttggagat ggcggcattc gcggtcgagt tggccggcga ggcgggcgtg ctcgtcgaag gagacgacaa tgttctcgac ggtggccgct ggcaggacga gtggctgtac gcccgcgcct acaccatcgc gggcggcagt tcggaaatca tgcgcaacct catcgccgag cggggacttg gcctaccccg cgatta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMEYGMRPEL AEFRAEVRAF VAEHAPAIPP RAGVRSAEND AEFKALQEWT GRLFEAGYVG ADWPVEFGGR GDFTPEHSII VGEELARAQA PSAQSGSVLA SHALIHYGTD EQRRRHLPEI RGGRELWCQL FSEPGAGSDL ASLRTRAVRH GDTYTIDGQK VWTTDGHWAR YGYLLARTDP DAPKHKGISA FILDMSAPGV SVRPLRELTG TSDFNEVFFD GVEVPADAMI GAPGQGWAIA NATLAHERTN VGGIVVKLRL AIDALIDLAR RVTIDGGPAI DSDRVRDRIG QFSAEVEALS ALTYANLTRW LRGRERMHDA AMGKLMFSEI NLEMAAFAVE LAGEAGVLVE GDDNVLDGGR WQDEWLYARA YTIAGGSSEI MRNLIAERGL GLPRD

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaatacggc atgagaccgg agttggcgga gttccgcgcc gaggtgcggg cgttcgtcgc cgagcacgcc ccggcgattc cgccccgggc gggggttcgc agcgctgaga acgacgccga gttcaaggcg ttgcaggagt ggaccggacg cctgttcgag gcgggctacg tgggagcgga ctggcctgtg gagttcgggg gtcgtggtga cttcacaccc gaacactcga tcatcgtggg ggaggagctt gctcgcgcgc aggcacccag cgcgcagagc ggcagcgtgc tcgcgtcgca cgcgctgatc cattacggca cagacgaaca gcgccggcga catctccccg agatccgcgg gggccgtgaa ctgtggtgcc agctgttcag cgagccgggt gcgggtagtg acctggcgtc gctacgcacc cgcgccgtac gccacggcga cacctacacg atcgatggcc agaaggtgtg gaccaccgac gggcactggg cgcgatacgg ctatctgctg gcacgcaccg accccgacgc accgaaacac aagggcatca gcgcattcat cctcgacatg tccgcaccgg gcgtcagtgt gcgaccgcta cgtgagctga cgggtacctc cgatttcaac gaggtgttct tcgacggtgt cgaggtgccg gccgacgcga tgatcggcgc acccggccag ggttgggcga tcgcgaacgc cacgctcgcc catgagcgca ccaatgtcgg tgggatcgtg gtgaagctgc ggctcgccat tgatgctttg atcgatctgg cccgtcgtgt cacgatcgac ggcgggccgg cgatcgacag cgaccgggtc cgcgaccgga tcggtcagtt cagcgccgag gtggaggcgt tgtcggcgtt gacatatgcc aacctgaccc gctggcttcg cggccgcgaa cgcatgcacg acgcggcgat gggcaagctg atgttcagcg aaatcaactt ggagatggcg gcattcgcgg tcgagttggc cggcgaggcg ggcgtgctcg tcgaaggaga cgacaatgtt ctcgacggtg gccgctggca ggacgagtgg ctgtacgccc gcgcctacac catcgcgggc ggcagttcgg aaatcatgcg caacctcatc gccgagcggg gacttggcct accccgcgat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa