SSGCID: Target Page

MyavA.01613.f

Putative acyl-CoA dehydrogenase

CENTER ID:	MyavA.01613.f
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01613.f.A1.GE29147	Full length( MyavA.01613.f )		1	365

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.1591
RefSeq:	YP_880864.1
UniProt:	A0A0H3A5Z5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVAEFAAWLA AFLPNDYYEN YRRYRWDLTL RRDYQRAAFE AGWIQPTWPR EHGGRSLGLR DAMEVRLEAA LRSAPKLPNI AGPNVAAPGI RQFGTPAQIE RLLVPLLRGD EWWALGMSEP EAGSDFAGLR TRAVRDGDVF RVNGHKIWTT QAHESRWCTL YARTDPEAPK HRGISCLILD LHSPGVRIEP IRMASISDET FCEVFLDDVE VPTENLLGPL HGGWDVALSS LHHERQMIWI MNWVEINRGL DAVRTANGAG PGHDIHTELG SLLADAEALR ATGYRALANE LAGRPSPEAD TMKLLGSLTL QRVWELAAAA AGPDSATDPD LLFERQDALA ATIYGGTSEV QRNIIAERLL GLPKG

NT Sequence

GTGGTCGCCG AGTTCGCGGC CTGGCTGGCG GCGTTCCTGC CGAATGACTA TTACGAGAAC TACCGGCGGT ACCGCTGGGA CCTCACGCTG CGCCGCGATT ACCAGCGGGC CGCGTTCGAG GCCGGGTGGA TCCAGCCGAC CTGGCCGCGC GAGCACGGCG GCCGGTCGCT GGGCCTGCGC GACGCGATGG AGGTCCGGCT GGAGGCGGCC CTGCGCTCGG CGCCCAAGCT GCCCAACATC GCCGGCCCCA ACGTCGCCGC CCCCGGCATC CGCCAGTTCG GCACGCCCGC CCAGATCGAG CGGCTGCTGG TGCCGCTGCT GCGCGGTGAC GAGTGGTGGG CGCTGGGCAT GTCCGAACCG GAAGCCGGCT CGGACTTCGC CGGTCTGCGC ACCCGCGCCG TGCGGGACGG CGACGTGTTC CGGGTCAACG GCCACAAGAT CTGGACCACC CAGGCTCACG AGTCGCGCTG GTGCACGCTG TATGCCCGCA CCGATCCCGA GGCGCCCAAG CACCGCGGCA TCTCCTGCCT CATCCTGGAT CTGCATTCGC CCGGGGTGCG GATCGAACCC ATCCGGATGG CGTCGATCTC CGACGAAACA TTCTGCGAGG TGTTCCTCGA CGACGTCGAG GTGCCGACGG AAAACCTGTT GGGGCCGCTG CACGGCGGCT GGGACGTCGC GCTGTCGTCG CTGCACCATG AACGCCAGAT GATCTGGATC ATGAACTGGG TCGAGATCAA CCGCGGACTC GACGCGGTGC GAACCGCCAA CGGCGCGGGG CCGGGCCACG ACATCCACAC CGAGCTCGGT TCCCTGCTGG CCGACGCCGA AGCCCTGCGC GCCACCGGGT ATCGGGCCCT GGCCAACGAA CTCGCGGGCC GGCCCAGCCC CGAAGCCGAC ACCATGAAGC TGCTGGGATC GCTTACCCTG CAACGTGTTT GGGAACTCGC GGCGGCCGCC GCGGGCCCCG ATTCGGCCAC CGACCCCGAT CTGCTGTTCG AACGTCAGGA CGCGCTGGCC GCGACCATCT ACGGCGGAAC GTCGGAGGTG CAGCGCAACA TCATCGCCGA GCGGCTGCTC GGGCTGCCGA AGGGA

Details for MyavA.01613.f.A1.GE29147

HARVESTED ON:	4/27/2010
SEQUENCED ON:	5/1/2010
EXPECTED MW:	42kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	92

Validated AA Sequence

MVVAEFAAWL AAFLPNDYYE NYRRYRWDLT LRRDYQRAAF EAGWIQPTWP REHGGRSLGL RDAMEVRLEA ALRSAPKLPN IAGPNVAAPG IRQFGTPAQI ERLLVPLLRG DEWWALGMSE PEAGSDFAGL RTRAVRDGDV FRVNGHKIWT TQAHESRWCT LYARTDPEAP KHRGISCLIL DLHSPGVRIE PIRMASISDE TFCEVFLDDV EVPTENLLGP LHGGWDVALS SLHHERQMIW IMNWVEINRG LDAVRTANGA GPGHDIHTEL GSLLADAEAL RATGYRALAN ELAGRPSPEA DTMKLLGSLT LQRVWELAAA AAGPDSATDP DLLFERQDAL AATIYGGTSE VQRNIIAE

Validated NT Sequence

gctcggcgat gatgttgcgc tgcacctccg acgttccgcc gtagatggtc gcggccagcg cgtcctgacg ttcgaacagc agatcggggt cggtggccga atcggggccc gcggcggccg ccgcgagttc ccaaacacgt tgcagggtaa gcgatcccag cagcttcatg gtgtcggctt cggggctggg ccggcccgcg agttcgttgg ccagggcccg atacccggtg gcgcgcaggg cttcggcgtc ggccagcagg gaaccgagct cggtgtggat gtcgtggccc ggccccgcgc cgttggcggt tcgcaccgcg tcgagtccgc ggttgatctc gacccagttc atgatccaga tcatctggcg ttcatggtgc agcgacgaca gcgcgacgtc ccagccgccg tgcagcggcc ccaacaggtt ttccgtcggc acctcgacgt cgtcgaggaa cacctcgcag aatgtttcgt cggagatcga cgccatccgg atgggttcga tccgcacccc gggcgaatgc agatccagga tgaggcagga gatgccgcgg tgcttgggcg cctcgggatc ggtgcgggca tacagcgtgc accagcgcga ctcgtgagcc tgggtggtcc agatcttgtg gccgttgacc cggaacacgt cgccgtcccg cacggcgcgg gtgcgcagac cggcgaagtc cgagccggct tccggttcgg acatgcccag cgcccaccac tcgtcaccgc gcagcagcgg caccagcagc cgctcgatct gggcgggcgt gccgaactgg cggatgccgg gggcggcgac gttggggccg gcgatgttgg gcagcttggg cgccgagcgc agggccgcct ccagccggac ctccatcgcg tcgcgcaggc ccagcgaccg gccgccgtgc tcgcgcggcc aggtcggctg gatccacccg gcctcgaacg cggcccgctg gtaatcgcgg cgcagcgtga ggtcccagcg gtaccgccgg tagttctcgt aatagtcatt cggcaggaac gccgccagcc aggccgcgaa ctcggcgacc accatcgaac cag

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVVAEFAAW LAAFLPNDYY ENYRRYRWDL TLRRDYQRAA FEAGWIQPTW PREHGGRSLG LRDAMEVRLE AALRSAPKLP NIAGPNVAAP GIRQFGTPAQ IERLLVPLLR GDEWWALGMS EPEAGSDFAG LRTRAVRDGD VFRVNGHKIW TTQAHESRWC TLYARTDPEA PKHRGISCLI LDLHSPGVRI EPIRMASISD ETFCEVFLDD VEVPTENLLG PLHGGWDVAL SSLHHERQMI WIMNWVEINR GLDAVRTANG AGPGHDIHTE LGSLLADAEA LRATGYRALA NELAGRPSPE ADTMKLLGSL TLQRVWELAA AAAGPDSATD PDLLFERQDA LAATIYGGTS EVQRNIIAER LLGLPKG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggtcgcc gagttcgcgg cctggctggc ggcgttcctg ccgaatgact attacgagaa ctaccggcgg taccgctggg acctcacgct gcgccgcgat taccagcggg ccgcgttcga ggccgggtgg atccagccga cctggccgcg cgagcacggc ggccggtcgc tgggcctgcg cgacgcgatg gaggtccggc tggaggcggc cctgcgctcg gcgcccaagc tgcccaacat cgccggcccc aacgtcgccg cccccggcat ccgccagttc ggcacgcccg cccagatcga gcggctgctg gtgccgctgc tgcgcggtga cgagtggtgg gcgctgggca tgtccgaacc ggaagccggc tcggacttcg ccggtctgcg cacccgcgcc gtgcgggacg gcgacgtgtt ccgggtcaac ggccacaaga tctggaccac ccaggctcac gagtcgcgct ggtgcacgct gtatgcccgc accgatcccg aggcgcccaa gcaccgcggc atctcctgcc tcatcctgga tctgcattcg cccggggtgc ggatcgaacc catccggatg gcgtcgatct ccgacgaaac attctgcgag gtgttcctcg acgacgtcga ggtgccgacg gaaaacctgt tggggccgct gcacggcggc tgggacgtcg cgctgtcgtc gctgcaccat gaacgccaga tgatctggat catgaactgg gtcgagatca accgcggact cgacgcggtg cgaaccgcca acggcgcggg gccgggccac gacatccaca ccgagctcgg ttccctgctg gccgacgccg aagccctgcg cgccaccggg tatcgggccc tggccaacga actcgcgggc cggcccagcc ccgaagccga caccatgaag ctgctgggat cgcttaccct gcaacgtgtt tgggaactcg cggcggccgc cgcgggcccc gattcggcca ccgaccccga tctgctgttc gaacgtcagg acgcgctggc cgcgaccatc tacggcggaa cgtcggaggt gcagcgcaac atcatcgccg agcggctgct cgggctgccg aagggaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa