MyavA.01613.e

Acyl-CoA dehydrogenase domain protein

CENTER ID: MyavA.01613.e
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01613.e.A1.GE29146 Full length( MyavA.01613.e ) 1 389
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.1241
RefSeq: YP_880513.1
UniProt: A0A0H2ZYV5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGKMDFCYP AEVERFRTEL RDWLSDNLTD ELVKARRAGG RDDAAFEMLR AWNQTMADAG WAAVSWPREY GGRGATVLEQ LVYTEETTRA RAPLPLNVIG LNNIAPAIMQ YGTEAQKQRL LPRMMRADDI WCQGMSEPDA GSDLAALRTR AVRDGDDFVV TGQKIWTSLG HRADWCQLYV RTDPDAPKHK GISCLIVDMT LPGIEARPLV TLGGDADFAE VFFHDVRVPA DALLGPLNAG WQVATTTLSH ERAGAARLYA EMQVRLEELV DDLAAAGTGA LDDPVTLRRL GELAVRIKYL EVLCQRSISA TLHGGSELVA LGSASLAKTV WGEIGQDLAA LAFDVLGSRD DGRAWANYRL TSRSLTIAGG TTQINKNITA QRVLGLPRK
NT Sequence
GTGTCCGGCA AAATGGATTT CTGCTATCCC GCGGAAGTGG AACGGTTCCG CACCGAGCTG CGTGACTGGC TGTCGGACAA CCTGACCGAC GAGCTGGTGA AAGCACGCCG CGCCGGCGGC CGTGACGACG CCGCGTTCGA GATGCTGCGG GCGTGGAACC AGACGATGGC CGACGCGGGA TGGGCCGCGG TGTCCTGGCC GCGCGAATAC GGTGGCCGCG GCGCGACGGT GCTCGAGCAG CTGGTCTACA CCGAGGAGAC CACCCGGGCG CGAGCTCCCT TGCCGCTCAA CGTTATTGGC TTGAACAACA TCGCGCCGGC GATCATGCAG TACGGCACCG AGGCGCAGAA GCAGCGGCTG CTGCCGCGCA TGATGCGCGC CGACGACATC TGGTGCCAGG GCATGTCGGA ACCCGATGCC GGGTCCGATC TCGCGGCGCT GCGCACCCGT GCGGTGCGCG ACGGTGACGA CTTCGTGGTC ACCGGGCAGA AGATCTGGAC CTCGCTGGGC CACCGCGCCG ACTGGTGCCA GCTTTATGTG CGCACCGATC CGGATGCGCC CAAGCACAAG GGGATCTCGT GCCTGATCGT CGACATGACG CTGCCCGGCA TCGAGGCCCG TCCGCTGGTC ACGCTGGGCG GCGACGCCGA TTTCGCCGAG GTCTTCTTTC ACGACGTGCG GGTGCCGGCG GACGCGTTGC TCGGCCCGCT CAATGCGGGC TGGCAGGTGG CCACCACCAC GCTGAGCCAC GAGCGGGCCG GGGCCGCCCG GTTGTACGCG GAGATGCAGG TGCGGCTGGA AGAGCTGGTG GACGATCTGG CCGCCGCCGG CACCGGCGCG CTGGACGACC CGGTCACGCT GCGGCGCCTC GGCGAACTCG CGGTGCGGAT CAAATATCTG GAAGTGCTGT GCCAGCGGTC GATTTCGGCC ACCCTGCACG GCGGGTCGGA ACTGGTGGCG CTGGGGTCGG CCAGCCTGGC CAAAACGGTG TGGGGCGAGA TCGGGCAGGA CCTGGCCGCG CTGGCCTTCG ACGTGCTGGG CTCCCGCGAC GACGGGCGGG CGTGGGCGAA CTACCGGCTG ACGTCGCGCT CGCTGACCAT CGCGGGCGGC ACCACGCAGA TCAACAAGAA CATCACCGCC CAACGGGTGT TGGGGTTGCC GCGCAAA
Details for MyavA.01613.e.A1.GE29146
HARVESTED ON: 4/27/2010
SEQUENCED ON: 5/1/2010
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 94
Validated AA Sequence
MVSGKMDFCY PAEVERFRTE LRDWLSDNLT DELVKARRAG GRDDAAFEML RAWNQTMADA GWAAVSWPRE YGGRGATVLE QLVYTEETTR ARAPLPLNVI GLNNIAPAIM QYGTEAQKQR LLPRMMRADD IWCQGMSEPD AGSDLAALRT RAVRDGDDFV VTGQKIWTSL GHRADWCQLY VRTDPDAPKH KGISCLIVDM TLPGIEARPL VTLGGDADFA EVFFHDVRVP ADALLGPLNA GWQVATTTLS HERAGAARLY AEMQVRLEEL VDDLAAAGTG ALDDPVTLRR LGELAVRIKY LEVLCQRSIS ATLHGGSELV ALGSASLAKT VWGEIGQDLA ALAFDVLGSR DDGRAWANYR LTSRSLTIAG GTTQINKNIX XQRVLGLPRK
Validated NT Sequence
ctggaagctc agacccaggg tcctggttcg atggtgtccg gcaaaatgga tttctgctat cccgcggaag tggaacggtt ccgcaccgag ctgcgtgact ggctgtcgga caacctgacc gacgagctgg tgaaagcacg ccgcgccggc ggccgtgacg acgccgcgtt cgagatgctg cgggcgtgga accagacgat ggccgacgcg ggatgggccg cggtgtcctg gccgcgcgaa tacggtggcc gcggcgcgac ggtgctcgag cagctggtct acaccgagga gaccacccgg gcgcgagctc ccttgccgct caacgttatt ggcttgaaca acatcgcgcc ggcgatcatg cagtacggca ccgaggcgca gaagcagcgg ctgctgccgc gcatgatgcg cgccgacgac atctggtgcc agggcatgtc ggaacccgat gccgggtccg atctcgcggc gctgcgcacc cgtgcggtgc gcgacggtga cgacttcgtg gtcaccgggc agaagatctg gacctcgctg ggccaccgcg ccgactggtg ccagctttat gtgcgcaccg atccggatgc gcccaagcac aaggggatct cgtgcctgat cgtcgacatg acgctgcccg gcatcgaggc ccgtccgctg gtcacgctgg gcggcgacgc cgatttcgcc gaggtcttct ttcacgacgt gcgggtgccg gcggacgcgt tgctcggccc gctcaatgcg ggctggcagg tggccaccac cacgctgagc cacgagcggg ccggggccgc ccggttgtac gcggagatgc aggtgcggct ggaagagctg gtggacgatc tggccgccgc cggcaccggc gcgctggacg acccggtcac gctgcggcgc ctcggcgaac tcgcggtgcg gatcaaatat ctggaagtgc tgtgccagcg gtcgatttcg gccaccctgc acggcgggtc ggaactggtg gcgctggggt cggccagcct ggccaaaacg gtgtggggcg agatcgggca ggacctggcc gcgctggcct tcgacgtgct gggctcccgc gacgacgggc gggcgtgggc gaactaccgg ctgacgtcgc gctcgctgac catcgcgggc ggcaccacgc agatcaacaa gaacatcncc ncccaacggg tgttggggtt gccgcgcaaa taat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSGKMDFC YPAEVERFRT ELRDWLSDNL TDELVKARRA GGRDDAAFEM LRAWNQTMAD AGWAAVSWPR EYGGRGATVL EQLVYTEETT RARAPLPLNV IGLNNIAPAI MQYGTEAQKQ RLLPRMMRAD DIWCQGMSEP DAGSDLAALR TRAVRDGDDF VVTGQKIWTS LGHRADWCQL YVRTDPDAPK HKGISCLIVD MTLPGIEARP LVTLGGDADF AEVFFHDVRV PADALLGPLN AGWQVATTTL SHERAGAARL YAEMQVRLEE LVDDLAAAGT GALDDPVTLR RLGELAVRIK YLEVLCQRSI SATLHGGSEL VALGSASLAK TVWGEIGQDL AALAFDVLGS RDDGRAWANY RLTSRSLTIA GGTTQINKNI TAQRVLGLPR K
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtccggc aaaatggatt tctgctatcc cgcggaagtg gaacggttcc gcaccgagct gcgtgactgg ctgtcggaca acctgaccga cgagctggtg aaagcacgcc gcgccggcgg ccgtgacgac gccgcgttcg agatgctgcg ggcgtggaac cagacgatgg ccgacgcggg atgggccgcg gtgtcctggc cgcgcgaata cggtggccgc ggcgcgacgg tgctcgagca gctggtctac accgaggaga ccacccgggc gcgagctccc ttgccgctca acgttattgg cttgaacaac atcgcgccgg cgatcatgca gtacggcacc gaggcgcaga agcagcggct gctgccgcgc atgatgcgcg ccgacgacat ctggtgccag ggcatgtcgg aacccgatgc cgggtccgat ctcgcggcgc tgcgcacccg tgcggtgcgc gacggtgacg acttcgtggt caccgggcag aagatctgga cctcgctggg ccaccgcgcc gactggtgcc agctttatgt gcgcaccgat ccggatgcgc ccaagcacaa ggggatctcg tgcctgatcg tcgacatgac gctgcccggc atcgaggccc gtccgctggt cacgctgggc ggcgacgccg atttcgccga ggtcttcttt cacgacgtgc gggtgccggc ggacgcgttg ctcggcccgc tcaatgcggg ctggcaggtg gccaccacca cgctgagcca cgagcgggcc ggggccgccc ggttgtacgc ggagatgcag gtgcggctgg aagagctggt ggacgatctg gccgccgccg gcaccggcgc gctggacgac ccggtcacgc tgcggcgcct cggcgaactc gcggtgcgga tcaaatatct ggaagtgctg tgccagcggt cgatttcggc caccctgcac ggcgggtcgg aactggtggc gctggggtcg gccagcctgg ccaaaacggt gtggggcgag atcgggcagg acctggccgc gctggccttc gacgtgctgg gctcccgcga cgacgggcgg gcgtgggcga actaccggct gacgtcgcgc tcgctgacca tcgcgggcgg caccacgcag atcaacaaga acatcaccgc ccaacgggtg ttggggttgc cgcgcaaaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa