MyavA.01613.c

Putative acyl-CoA dehydrogenase

CENTER ID: MyavA.01613.c
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01613.c.A1.GE29144 Full length( MyavA.01613.c ) 1 365
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.877
RefSeq: YP_880164.1
UniProt: A0A0H2ZVU1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNVEQFRADL RAWLDDNDLT PGPDHSLQGH MKQFARVSRA LYDADWMRYG WPAEVGGLGG PAVLRAVVGE EVVGRRLAEP GPYSMLEVLA PTMIDYAPAE LAAEMVPRLL RGEEHWCQGF SEPGSGSDLA SLTTRATQDG DHWIVNGQKV WTSFAQFSTR CVLLTRTAPG YDGITAFFVD LDTPGITIRP LRTMHGVDEF CEVYYDDVAI PSSRMLGRPG DGWRLAMDLL PYERSSCFWQ RIAYLYSRFD DLIAEVSAAG EPDESSLGAA YLALHTLRCR SRATQHRMRD GARLGPDTSI DKVLLATAEQ RLYDTVRDLL PGTLELENTP WRSEYLYSRA ATIYGGTAEI QRNIIARRLL DLGKE
NT Sequence
GTGAATGTCG AGCAGTTCCG GGCCGACCTG CGCGCGTGGC TCGACGACAA CGATCTGACC CCCGGGCCCG ACCATTCCCT GCAGGGCCAC ATGAAGCAGT TCGCCCGGGT CAGCCGCGCG CTCTACGACG CCGACTGGAT GCGGTACGGG TGGCCGGCCG AAGTCGGTGG CCTGGGCGGA CCCGCGGTGC TGCGGGCGGT CGTCGGCGAG GAAGTGGTGG GTCGCCGGCT GGCCGAACCC GGACCCTACT CGATGCTCGA GGTGCTGGCT CCCACCATGA TCGACTACGC CCCCGCCGAA CTGGCCGCGG AGATGGTGCC GCGGCTGCTG CGCGGCGAAG AGCACTGGTG TCAAGGCTTT TCCGAGCCGG GGTCGGGCAG CGACCTGGCG TCGCTGACCA CCCGCGCGAC CCAGGACGGC GACCACTGGA TCGTCAACGG GCAGAAGGTC TGGACCAGCT TCGCGCAGTT CTCCACCCGG TGCGTGCTGC TGACCCGCAC CGCGCCCGGC TACGACGGGA TCACCGCCTT CTTCGTCGAC CTGGACACGC CGGGTATCAC CATCCGGCCG CTGCGCACCA TGCACGGCGT CGACGAATTC TGCGAGGTGT ACTACGACGA CGTGGCGATT CCGTCGAGCC GCATGCTCGG CCGGCCCGGC GACGGCTGGC GCCTGGCCAT GGATCTGCTG CCGTATGAAC GGTCCTCCTG CTTCTGGCAG CGCATCGCCT ACCTCTACTC GCGTTTCGAC GATCTGATCG CCGAGGTCTC GGCGGCCGGG GAGCCCGACG AATCCTCCCT GGGCGCGGCC TATTTGGCGC TGCACACGTT GCGCTGCCGG TCGCGCGCCA CCCAGCACCG GATGCGCGAC GGGGCCCGGC TGGGGCCCGA CACCTCGATC GACAAGGTGC TGCTGGCCAC CGCCGAGCAG CGGCTCTACG ACACCGTGCG CGACCTGTTG CCGGGCACCC TGGAGCTCGA AAACACGCCG TGGCGCTCGG AATACCTGTA CTCGCGCGCG GCCACCATCT ACGGCGGTAC CGCCGAGATC CAGCGCAACA TCATCGCCCG CCGGCTGCTC GATCTCGGGA AGGAG
Details for MyavA.01613.c.A1.GE29144
HARVESTED ON: 4/27/2010
SEQUENCED ON: 5/1/2010
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MVNVEQFRAD LRAWLDDNDL TPGPDHSLQG HMKQFARVSR ALYDADWMRY GWPAEVGGLG GPAVLRAVVG EEVVGRRLAE PGPYSMLEVL APTMIDYAPA ELAAEMVPRL LRGEEHWCQG FSEPGSGSDL ASLTTRATQD GDHWIVNGQK VWTSFAQFST RCVLLTRTAP GYDGITAFFV DLDTPGITIR PLRTMHGVDE FCEVYYDDVA IPSSRMLGRP GDGWRLAMDL LPYERSSCFW QRIAYLYSRF DDLIAEVSAA GEPDESSLGA AYLALHTLRC RSRATQHRMR DGARLGPDTS IDKVLLATAE QRLYDTVRDL LPGTLELENT PWRSEYLYSR AATIYGGTAE IQRNIIARRL LDLG
Validated NT Sequence
tcccgagatc gagcagccgg cgggcgatga tgttgcgctg gatctcggcg gtaccgccgt agatggtggc cgcgcgcgag tacaggtatt ccgagcgcca cggcgtgttt tcgagctcca gggtgcccgg caacaggtcg cgcacggtgt cgtagagccg ctgctcggcg gtggccagca gcaccttgtc gatcgaggtg tcgggcccca gccgggcccc gtcgcgcatc cggtgctggg tggcgcgcga ccggcagcgc aacgtgtgca gcgccaaata ggccgcgccc agggaggatt cgtcgggctc cccggccgcc gagacctcgg cgatcagatc gtcgaaacgc gagtagaggt aggcgatgcg ctgccagaag caggaggacc gttcatacgg cagcagatcc atggccaggc gccagccgtc gccgggccgg ccgagcatgc ggctcgacgg aatcgccacg tcgtcgtagt acacctcgca gaattcgtcg acgccgtgca tggtgcgcag cggccggatg gtgatacccg gcgtgtccag gtcgacgaag aaggcggtga tcccgtcgta gccgggcgcg gtgcgggtca gcagcacgca ccgggtggag aactgcgcga agctggtcca gaccttctgc ccgttgacga tccagtggtc gccgtcctgg gtcgcgcggg tggtcagcga cgccaggtcg ctgcccgacc ccggctcgga aaagccttga caccagtgct cttcgccgcg cagcagccgc ggcaccatct ccgcggccag ttcggcgggg gcgtagtcga tcatggtggg agccagcacc tcgagcatcg agtagggtcc gggttcggcc agccggcgac ccaccacttc ctcgccgacg accgcccgca gcaccgcggg tccgcccagg ccaccgactt cggccggcca cccgtaccgc atccagtcgg cgtcgtagag cgcgcggctg acccgggcga actgcttcat gtggccctgc agggaatggt cgggcccggg ggtcagatcg ttgtcgtcga gccacgcgcg caggtcggcc cggaactgct cgacattcac catcgaacca ggaccctggg tctgagcttc cagggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVNVEQFRA DLRAWLDDND LTPGPDHSLQ GHMKQFARVS RALYDADWMR YGWPAEVGGL GGPAVLRAVV GEEVVGRRLA EPGPYSMLEV LAPTMIDYAP AELAAEMVPR LLRGEEHWCQ GFSEPGSGSD LASLTTRATQ DGDHWIVNGQ KVWTSFAQFS TRCVLLTRTA PGYDGITAFF VDLDTPGITI RPLRTMHGVD EFCEVYYDDV AIPSSRMLGR PGDGWRLAMD LLPYERSSCF WQRIAYLYSR FDDLIAEVSA AGEPDESSLG AAYLALHTLR CRSRATQHRM RDGARLGPDT SIDKVLLATA EQRLYDTVRD LLPGTLELEN TPWRSEYLYS RAATIYGGTA EIQRNIIARR LLDLGKE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaatgtc gagcagttcc gggccgacct gcgcgcgtgg ctcgacgaca acgatctgac ccccgggccc gaccattccc tgcagggcca catgaagcag ttcgcccggg tcagccgcgc gctctacgac gccgactgga tgcggtacgg gtggccggcc gaagtcggtg gcctgggcgg acccgcggtg ctgcgggcgg tcgtcggcga ggaagtggtg ggtcgccggc tggccgaacc cggaccctac tcgatgctcg aggtgctggc tcccaccatg atcgactacg cccccgccga actggccgcg gagatggtgc cgcggctgct gcgcggcgaa gagcactggt gtcaaggctt ttccgagccg gggtcgggca gcgacctggc gtcgctgacc acccgcgcga cccaggacgg cgaccactgg atcgtcaacg ggcagaaggt ctggaccagc ttcgcgcagt tctccacccg gtgcgtgctg ctgacccgca ccgcgcccgg ctacgacggg atcaccgcct tcttcgtcga cctggacacg ccgggtatca ccatccggcc gctgcgcacc atgcacggcg tcgacgaatt ctgcgaggtg tactacgacg acgtggcgat tccgtcgagc cgcatgctcg gccggcccgg cgacggctgg cgcctggcca tggatctgct gccgtatgaa cggtcctcct gcttctggca gcgcatcgcc tacctctact cgcgtttcga cgatctgatc gccgaggtct cggcggccgg ggagcccgac gaatcctccc tgggcgcggc ctatttggcg ctgcacacgt tgcgctgccg gtcgcgcgcc acccagcacc ggatgcgcga cggggcccgg ctggggcccg acacctcgat cgacaaggtg ctgctggcca ccgccgagca gcggctctac gacaccgtgc gcgacctgtt gccgggcacc ctggagctcg aaaacacgcc gtggcgctcg gaatacctgt actcgcgcgc ggccaccatc tacggcggta ccgccgagat ccagcgcaac atcatcgccc gccggctgct cgatctcggg aaggagaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa