MyavA.01582.d

Short chain dehydrogenase

CENTER ID: MyavA.01582.d
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MyavA.01582.d.A1.GE29139 Full length( MyavA.01582.d ) 1 307
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MyavA.01582.d.A1.PS00732 Full length( MyavA.01582.d ) 1 307
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_239168
PATRIC ID: fig|243243.7.peg.2576
RefSeq: YP_881830.1
UniProt: A0A0H2ZVA5
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGLLDGKVA LVTGAGHGIG RGHALELAKH GATVIVNDLG TSLSGEGTGK VADEVVAIIE SRGGTAVSDF SDVGDEEQVD LAVERAYSQL GRLDIVVNNA GIVRDKAIWN MTADDFDLVM RVHVRGSWLT SRAVARKWRA ESKESGGKVY GRIINTTSGA GLHGHFGQTN YSAAKAAIVG LTQTLSLELA SIGATVNAIS PGGRTRMSAS MPGAQAPIEP DERAEDEFDP KDPSLGSPVV AWLASPEAGH VSGQVIRAMG EHLQLLKGWH PVASVSNGEK RWDAQKLGAI MATDVFGTRN TGLRLGG
NT Sequence
ATGACCGGAC TGCTCGATGG CAAAGTCGCC CTGGTGACCG GGGCGGGGCA CGGCATCGGC CGGGGCCACG CTCTCGAACT CGCCAAGCAC GGCGCCACCG TGATCGTCAA CGACCTCGGC ACCAGCCTGT CCGGCGAGGG CACCGGCAAG GTGGCCGACG AGGTGGTGGC GATCATCGAA AGCCGCGGTG GTACCGCTGT TTCCGACTTC AGCGATGTCG GCGACGAGGA GCAGGTCGAC CTCGCCGTCG AGCGTGCCTA CTCGCAGCTG GGGCGGCTGG ACATCGTCGT CAACAACGCA GGAATCGTTC GCGACAAGGC GATCTGGAAC ATGACCGCCG ACGATTTCGA CCTGGTGATG CGGGTGCACG TTCGCGGTAG CTGGCTCACC AGCCGCGCGG TCGCTCGCAA GTGGCGCGCG GAATCGAAGG AATCCGGCGG AAAGGTCTAC GGCCGAATCA TCAACACCAC CTCCGGCGCC GGTCTGCACG GCCACTTCGG GCAGACCAAT TACAGCGCGG CCAAGGCCGC AATCGTCGGT CTGACCCAGA CACTGAGCCT GGAGTTGGCG TCGATCGGCG CCACCGTCAA CGCGATCAGC CCGGGTGGGC GCACCCGGAT GTCGGCCTCC ATGCCCGGCG CGCAGGCTCC GATCGAGCCC GACGAGCGCG CGGAGGACGA GTTCGACCCC AAGGACCCCT CACTGGGGTC GCCGGTGGTG GCCTGGCTGG CCAGCCCCGA GGCCGGCCAC GTCAGCGGTC AAGTGATCCG TGCGATGGGC GAACATCTCC AGCTCCTGAA GGGCTGGCAC CCCGTCGCGT CTGTCTCGAA TGGCGAAAAG CGCTGGGATG CACAGAAACT CGGCGCCATC ATGGCCACCG ACGTGTTTGG CACCCGAAAC ACGGGCCTGC GGCTCGGAGG A
Details for MyavA.01582.d.A1.GE29139
HARVESTED ON: 4/27/2010
SEQUENCED ON: 5/1/2010
EXPECTED MW: 34kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTGLLDGKV ALVTGAGHGI GRGHALELAK HGATVIVNDL GTSLSGEGTG KVADEVVAII ESRGGTAVSD FSDVGDEEQV DLAVERAYSQ LGRLDIVVNN AGIVRDKAIW NMTADDFDLV MRVHVRGSWL TSRAVARKWR AESKESGGKV YGRIINTTSG AGLHGHFGQT NYSAAKAAIV GLTQTLSLEL ASIGATVNAI SPGGRTRMSA SMPGAQAPIE PDERAEDEFD PKDPSLGSPV VAWLASPEAG HVSGQVIRAM GEHLQLLKGW HPVASVSNGE KRWDAQKLGA IMATDVFGTR NTGLRLGG
Validated NT Sequence
ttattatcct ccgagccgca ggcccgtgtt tcgggtgcca aacacgtcgg tggccatgat ggcgccgagt ttctgtgcat cccagcgctt ttcgccattc gagacagacg cgacggggtg ccagcccttc aggagctgga gatgttcgcc catcgcacgg atcacttgac cgctgacgtg gccggcctcg gggctggcca gccaggccac caccggcgac cccagtgagg ggtccttggg gtcgaactcg tcctccgcgc gctcgtcggg ctcgatcgga gcctgcgcgc cgggcatgga ggccgacatc cgggtgcgcc cacccgggct gatcgcgttg acggtggcgc cgatcgacgc caactccagg ctcagtgtct gggtcagacc gacgattgcg gccttggccg cgctgtaatt ggtctgcccg aagtggccgt gcagaccggc gccggaggtg gtgttgatga ttcggccgta gacctttccg ccggattcct tcgattccgc gcgccacttg cgagcgaccg cgcggctggt gagccagcta ccgcgaacgt gcacccgcat caccaggtcg aaatcgtcgg cggtcatgtt ccagatcgcc ttgtcgcgaa cgattcctgc gttgttgacg acgatgtcca gccgccccag ctgcgagtag gcacgctcga cggcgaggtc gacctgctcc tcgtcgccga catcgctgaa gtcggaaaca gcggtaccac cgcggctttc gatgatcgcc accacctcgt cggccacctt gccggtgccc tcgccggaca ggctggtgcc gaggtcgttg acgatcacgg tggcgccgtg cttggcgagt tcgagagcgt ggccccggcc gatgccgtgc cccgccccgg tcaccagggc gactttgcca tcgagcagtc cggtcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTGLLDGKV ALVTGAGHGI GRGHALELAK HGATVIVNDL GTSLSGEGTG KVADEVVAII ESRGGTAVSD FSDVGDEEQV DLAVERAYSQ LGRLDIVVNN AGIVRDKAIW NMTADDFDLV MRVHVRGSWL TSRAVARKWR AESKESGGKV YGRIINTTSG AGLHGHFGQT NYSAAKAAIV GLTQTLSLEL ASIGATVNAI SPGGRTRMSA SMPGAQAPIE PDERAEDEFD PKDPSLGSPV VAWLASPEAG HVSGQVIRAM GEHLQLLKGW HPVASVSNGE KRWDAQKLGA IMATDVFGTR NTGLRLGG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccggactg ctcgatggca aagtcgccct ggtgaccggg gcggggcacg gcatcggccg gggccacgct ctcgaactcg ccaagcacgg cgccaccgtg atcgtcaacg acctcggcac cagcctgtcc ggcgagggca ccggcaaggt ggccgacgag gtggtggcga tcatcgaaag ccgcggtggt accgctgttt ccgacttcag cgatgtcggc gacgaggagc aggtcgacct cgccgtcgag cgtgcctact cgcagctggg gcggctggac atcgtcgtca acaacgcagg aatcgttcgc gacaaggcga tctggaacat gaccgccgac gatttcgacc tggtgatgcg ggtgcacgtt cgcggtagct ggctcaccag ccgcgcggtc gctcgcaagt ggcgcgcgga atcgaaggaa tccggcggaa aggtctacgg ccgaatcatc aacaccacct ccggcgccgg tctgcacggc cacttcgggc agaccaatta cagcgcggcc aaggccgcaa tcgtcggtct gacccagaca ctgagcctgg agttggcgtc gatcggcgcc accgtcaacg cgatcagccc gggtgggcgc acccggatgt cggcctccat gcccggcgcg caggctccga tcgagcccga cgagcgcgcg gaggacgagt tcgaccccaa ggacccctca ctggggtcgc cggtggtggc ctggctggcc agccccgagg ccggccacgt cagcggtcaa gtgatccgtg cgatgggcga acatctccag ctcctgaagg gctggcaccc cgtcgcgtct gtctcgaatg gcgaaaagcg ctgggatgca cagaaactcg gcgccatcat ggccaccgac gtgtttggca cccgaaacac gggcctgcgg ctcggaggaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MyavA.01582.d.A1.PS00732
PURIFICATION DATe: 8/23/2010
CONCENTRATION: 68.13mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTGLLDGKV ALVTGAGHGI GRGHALELAK HGATVIVNDL GTSLSGEGTG KVADEVVAII ESRGGTAVSD FSDVGDEEQV DLAVERAYSQ LGRLDIVVNN AGIVRDKAIW NMTADDFDLV MRVHVRGSWL TSRAVARKWR AESKESGGKV YGRIINTTSG AGLHGHFGQT NYSAAKAAIV GLTQTLSLEL ASIGATVNAI SPGGRTRMSA SMPGAQAPIE PDERAEDEFD PKDPSLGSPV VAWLASPEAG HVSGQVIRAM GEHLQLLKGW HPVASVSNGE KRWDAQKLGA IMATDVFGTR NTGLRLGG
Validated NT Sequence
ttattatcct ccgagccgca ggcccgtgtt tcgggtgcca aacacgtcgg tggccatgat ggcgccgagt ttctgtgcat cccagcgctt ttcgccattc gagacagacg cgacggggtg ccagcccttc aggagctgga gatgttcgcc catcgcacgg atcacttgac cgctgacgtg gccggcctcg gggctggcca gccaggccac caccggcgac cccagtgagg ggtccttggg gtcgaactcg tcctccgcgc gctcgtcggg ctcgatcgga gcctgcgcgc cgggcatgga ggccgacatc cgggtgcgcc cacccgggct gatcgcgttg acggtggcgc cgatcgacgc caactccagg ctcagtgtct gggtcagacc gacgattgcg gccttggccg cgctgtaatt ggtctgcccg aagtggccgt gcagaccggc gccggaggtg gtgttgatga ttcggccgta gacctttccg ccggattcct tcgattccgc gcgccacttg cgagcgaccg cgcggctggt gagccagcta ccgcgaacgt gcacccgcat caccaggtcg aaatcgtcgg cggtcatgtt ccagatcgcc ttgtcgcgaa cgattcctgc gttgttgacg acgatgtcca gccgccccag ctgcgagtag gcacgctcga cggcgaggtc gacctgctcc tcgtcgccga catcgctgaa gtcggaaaca gcggtaccac cgcggctttc gatgatcgcc accacctcgt cggccacctt gccggtgccc tcgccggaca ggctggtgcc gaggtcgttg acgatcacgg tggcgccgtg cttggcgagt tcgagagcgt ggccccggcc gatgccgtgc cccgccccgg tcaccagggc gactttgcca tcgagcagtc cggtcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTGLLDGKV ALVTGAGHGI GRGHALELAK HGATVIVNDL GTSLSGEGTG KVADEVVAII ESRGGTAVSD FSDVGDEEQV DLAVERAYSQ LGRLDIVVNN AGIVRDKAIW NMTADDFDLV MRVHVRGSWL TSRAVARKWR AESKESGGKV YGRIINTTSG AGLHGHFGQT NYSAAKAAIV GLTQTLSLEL ASIGATVNAI SPGGRTRMSA SMPGAQAPIE PDERAEDEFD PKDPSLGSPV VAWLASPEAG HVSGQVIRAM GEHLQLLKGW HPVASVSNGE KRWDAQKLGA IMATDVFGTR NTGLRLGG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGGACTG CTCGATGGCA AAGTCGCCCT GGTGACCGGG GCGGGGCACG GCATCGGCCG GGGCCACGCT CTCGAACTCG CCAAGCACGG CGCCACCGTG ATCGTCAACG ACCTCGGCAC CAGCCTGTCC GGCGAGGGCA CCGGCAAGGT GGCCGACGAG GTGGTGGCGA TCATCGAAAG CCGCGGTGGT ACCGCTGTTT CCGACTTCAG CGATGTCGGC GACGAGGAGC AGGTCGACCT CGCCGTCGAG CGTGCCTACT CGCAGCTGGG GCGGCTGGAC ATCGTCGTCA ACAACGCAGG AATCGTTCGC GACAAGGCGA TCTGGAACAT GACCGCCGAC GATTTCGACC TGGTGATGCG GGTGCACGTT CGCGGTAGCT GGCTCACCAG CCGCGCGGTC GCTCGCAAGT GGCGCGCGGA ATCGAAGGAA TCCGGCGGAA AGGTCTACGG CCGAATCATC AACACCACCT CCGGCGCCGG TCTGCACGGC CACTTCGGGC AGACCAATTA CAGCGCGGCC AAGGCCGCAA TCGTCGGTCT GACCCAGACA CTGAGCCTGG AGTTGGCGTC GATCGGCGCC ACCGTCAACG CGATCAGCCC GGGTGGGCGC ACCCGGATGT CGGCCTCCAT GCCCGGCGCG CAGGCTCCGA TCGAGCCCGA CGAGCGCGCG GAGGACGAGT TCGACCCCAA GGACCCCTCA CTGGGGTCGC CGGTGGTGGC CTGGCTGGCC AGCCCCGAGG CCGGCCACGT CAGCGGTCAA GTGATCCGTG CGATGGGCGA ACATCTCCAG CTCCTGAAGG GCTGGCACCC CGTCGCGTCT GTCTCGAATG GCGAAAAGCG CTGGGATGCA CAGAAACTCG GCGCCATCAT GGCCACCGAC GTGTTTGGCA CCCGAAACAC GGGCCTGCGG CTCGGAGGAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A