MyavA.01582.c
Short chain dehydrogenase
| CENTER ID: | MyavA.01582.c |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01582.c.A1.GE29138 | Full length( MyavA.01582.c ) | 1 | 307 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01582.c.A1.PW29043 | Full length( MyavA.01582.c ) | 1 | 307 |
Structures
7ULH
DEPOSITED: 4/4/2022
DETERMINATION: XRay
CLONE: MyavA.01582.c.A1.GE29138
PROTEIN: MyavA.01582.c.A1.PW29043
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_239168 |
| PATRIC ID: | fig|243243.7.peg.1895 |
| RefSeq: | YP_881156.1 |
| UniProt: | A0A0H2ZRP4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGLLDGKVA LVTGAGHGIG RGHALELAKH GATVIVNDLG TSLSGEGTGK VADEVVTVIE SRGGKAVSDF SDVGDEEQVD LAVERAYSQL GRLDIVVNNA GIVRDKAIWN MTVDDFDLVM RVHVRGSWLT SRAVARKWRA ESKESGAKVY GRIINTTSGA GLHGHFGQTN YSAAKAAIVG LTQTLSLELA SIGATVNAIS PGGRTRMSAS MPGAAAPIEP DERSEDEFDP KDPSLGSPVV AWLASPEAGH ISGQVIRAIG EHLQLLKGWH PVASVSNGQK RWDANKLGAI MAADIFGTRN TGLRLGG
NT Sequence
ATGACAGGTC TACTCGACGG CAAGGTCGCT CTGGTGACCG GCGCCGGCCA CGGCATCGGC CGCGGTCATG CACTCGAACT GGCCAAGCAC GGCGCGACCG TGATTGTCAA CGACCTGGGC ACCAGCCTGT CCGGTGAGGG CACCGGCAAG GTGGCCGACG AGGTGGTGAC GGTCATCGAA AGTCGAGGCG GCAAAGCGGT TTCCGACTTC AGCGACGTGG GCGACGAGGA ACAGGTCGAC CTCGCGGTGG AGCGGGCCTA CTCGCAGCTG GGCCGGTTGG ACATCGTGGT CAACAATGCG GGCATCGTGC GCGACAAGGC GATCTGGAAC ATGACCGTCG ACGACTTCGA CCTGGTGATG CGGGTGCACG TCCGCGGCAG CTGGCTCACC AGCCGCGCCG TCGCCCGCAA GTGGCGTGCG GAATCCAAGG AATCCGGCGC AAAGGTGTAC GGCCGCATCA TCAACACCAC CTCCGGAGCG GGCCTGCACG GCCATTTCGG CCAGACGAAC TACAGCGCGG CCAAGGCCGC GATCGTCGGA CTTACCCAGA CCCTGAGCCT GGAACTCGCG TCGATCGGTG CCACGGTGAA TGCCATCAGC CCGGGCGGTC GCACCCGGAT GTCGGCGTCC ATGCCGGGAG CGGCCGCACC CATCGAGCCC GACGAGCGGT CCGAGGACGA GTTCGACCCG AAGGATCCGT CTTTGGGCTC GCCGGTGGTG GCCTGGCTGG CCAGCCCCGA GGCCGGACAC ATCAGCGGCC AGGTGATCCG CGCCATCGGC GAACATCTCC AGCTACTCAA GGGCTGGCAT CCGGTGGCGT CGGTGTCCAA CGGCCAAAAA CGCTGGGATG CCAACAAACT CGGCGCGATC ATGGCGGCCG ATATATTCGG TACCCGTAAC ACCGGCCTGC GCCTGGGAGG T
Details for MyavA.01582.c.A1.GE29138
| HARVESTED ON: | 4/27/2010 |
| SEQUENCED ON: | 5/1/2010 |
| EXPECTED MW: | 34kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTGLLDGKV ALVTGAGHGI GRGHALELAK HGATVIVNDL GTSLSGEGTG KVADEVVTVI ESRGGKAVSD FSDVGDEEQV DLAVERAYSQ LGRLDIVVNN AGIVRDKAIW NMTVDDFDLV MRVHVRGSWL TSRAVARKWR AESKESGAKV YGRIINTTSG AGLHGHFGQT NYSAAKAAIV GLTQTLSLEL ASIGATVNAI SPGGRTRMSA SMPGAAAPIE PDERSEDEFD PKDPSLGSPV VAWLASPEAG HISGQVIRAI GEHLQLLKGW HPVASVSNGQ KRWDANKLGA IMAADIFGTR NTGLRLGG
Validated NT Sequence
ttattaacct cccaggcgca ggccggtgtt acgggtaccg aatatatcgg ccgccatgat cgcgccgagt ttgttggcat cccagcgttt ttggccgttg gacaccgacg ccaccggatg ccagcccttg agtagctgga gatgttcgcc gatggcgcgg atcacctggc cgctgatgtg tccggcctcg gggctggcca gccaggccac caccggcgag cccaaagacg gatccttcgg gtcgaactcg tcctcggacc gctcgtcggg ctcgatgggt gcggccgctc ccggcatgga cgccgacatc cgggtgcgac cgcccgggct gatggcattc accgtggcac cgatcgacgc gagttccagg ctcagggtct gggtaagtcc gacgatcgcg gccttggccg cgctgtagtt cgtctggccg aaatggccgt gcaggcccgc tccggaggtg gtgttgatga tgcggccgta cacctttgcg ccggattcct tggattccgc acgccacttg cgggcgacgg cgcggctggt gagccagctg ccgcggacgt gcacccgcat caccaggtcg aagtcgtcga cggtcatgtt ccagatcgcc ttgtcgcgca cgatgcccgc attgttgacc acgatgtcca accggcccag ctgcgagtag gcccgctcca ccgcgaggtc gacctgttcc tcgtcgccca cgtcgctgaa gtcggaaacc gctttgccgc ctcgactttc gatgaccgtc accacctcgt cggccacctt gccggtgccc tcaccggaca ggctggtgcc caggtcgttg acaatcacgg tcgcgccgtg cttggccagt tcgagtgcat gaccgcggcc gatgccgtgg ccggcgccgg tcaccagagc gaccttgccg tcgagtagac ctgtcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTGLLDGKV ALVTGAGHGI GRGHALELAK HGATVIVNDL GTSLSGEGTG KVADEVVTVI ESRGGKAVSD FSDVGDEEQV DLAVERAYSQ LGRLDIVVNN AGIVRDKAIW NMTVDDFDLV MRVHVRGSWL TSRAVARKWR AESKESGAKV YGRIINTTSG AGLHGHFGQT NYSAAKAAIV GLTQTLSLEL ASIGATVNAI SPGGRTRMSA SMPGAAAPIE PDERSEDEFD PKDPSLGSPV VAWLASPEAG HISGQVIRAI GEHLQLLKGW HPVASVSNGQ KRWDANKLGA IMAADIFGTR NTGLRLGG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaggtcta ctcgacggca aggtcgctct ggtgaccggc gccggccacg gcatcggccg cggtcatgca ctcgaactgg ccaagcacgg cgcgaccgtg attgtcaacg acctgggcac cagcctgtcc ggtgagggca ccggcaaggt ggccgacgag gtggtgacgg tcatcgaaag tcgaggcggc aaagcggttt ccgacttcag cgacgtgggc gacgaggaac aggtcgacct cgcggtggag cgggcctact cgcagctggg ccggttggac atcgtggtca acaatgcggg catcgtgcgc gacaaggcga tctggaacat gaccgtcgac gacttcgacc tggtgatgcg ggtgcacgtc cgcggcagct ggctcaccag ccgcgccgtc gcccgcaagt ggcgtgcgga atccaaggaa tccggcgcaa aggtgtacgg ccgcatcatc aacaccacct ccggagcggg cctgcacggc catttcggcc agacgaacta cagcgcggcc aaggccgcga tcgtcggact tacccagacc ctgagcctgg aactcgcgtc gatcggtgcc acggtgaatg ccatcagccc gggcggtcgc acccggatgt cggcgtccat gccgggagcg gccgcaccca tcgagcccga cgagcggtcc gaggacgagt tcgacccgaa ggatccgtct ttgggctcgc cggtggtggc ctggctggcc agccccgagg ccggacacat cagcggccag gtgatccgcg ccatcggcga acatctccag ctactcaagg gctggcatcc ggtggcgtcg gtgtccaacg gccaaaaacg ctgggatgcc aacaaactcg gcgcgatcat ggcggccgat atattcggta cccgtaacac cggcctgcgc ctgggaggta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MyavA.01582.c.A1.PW29043
| PURIFICATION DATe: | 7/26/2010 |
| CONCENTRATION: | 31.05mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTGLLDGKV ALVTGAGHGI GRGHALELAK HGATVIVNDL GTSLSGEGTG KVADEVVTVI ESRGGKAVSD FSDVGDEEQV DLAVERAYSQ LGRLDIVVNN AGIVRDKAIW NMTVDDFDLV MRVHVRGSWL TSRAVARKWR AESKESGAKV YGRIINTTSG AGLHGHFGQT NYSAAKAAIV GLTQTLSLEL ASIGATVNAI SPGGRTRMSA SMPGAAAPIE PDERSEDEFD PKDPSLGSPV VAWLASPEAG HISGQVIRAI GEHLQLLKGW HPVASVSNGQ KRWDANKLGA IMAADIFGTR NTGLRLGG
Validated NT Sequence
ttattaacct cccaggcgca ggccggtgtt acgggtaccg aatatatcgg ccgccatgat cgcgccgagt ttgttggcat cccagcgttt ttggccgttg gacaccgacg ccaccggatg ccagcccttg agtagctgga gatgttcgcc gatggcgcgg atcacctggc cgctgatgtg tccggcctcg gggctggcca gccaggccac caccggcgag cccaaagacg gatccttcgg gtcgaactcg tcctcggacc gctcgtcggg ctcgatgggt gcggccgctc ccggcatgga cgccgacatc cgggtgcgac cgcccgggct gatggcattc accgtggcac cgatcgacgc gagttccagg ctcagggtct gggtaagtcc gacgatcgcg gccttggccg cgctgtagtt cgtctggccg aaatggccgt gcaggcccgc tccggaggtg gtgttgatga tgcggccgta cacctttgcg ccggattcct tggattccgc acgccacttg cgggcgacgg cgcggctggt gagccagctg ccgcggacgt gcacccgcat caccaggtcg aagtcgtcga cggtcatgtt ccagatcgcc ttgtcgcgca cgatgcccgc attgttgacc acgatgtcca accggcccag ctgcgagtag gcccgctcca ccgcgaggtc gacctgttcc tcgtcgccca cgtcgctgaa gtcggaaacc gctttgccgc ctcgactttc gatgaccgtc accacctcgt cggccacctt gccggtgccc tcaccggaca ggctggtgcc caggtcgttg acaatcacgg tcgcgccgtg cttggccagt tcgagtgcat gaccgcggcc gatgccgtgg ccggcgccgg tcaccagagc gaccttgccg tcgagtagac ctgtcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTGLLDGKV ALVTGAGHGI GRGHALELAK HGATVIVNDL GTSLSGEGTG KVADEVVTVI ESRGGKAVSD FSDVGDEEQV DLAVERAYSQ LGRLDIVVNN AGIVRDKAIW NMTVDDFDLV MRVHVRGSWL TSRAVARKWR AESKESGAKV YGRIINTTSG AGLHGHFGQT NYSAAKAAIV GLTQTLSLEL ASIGATVNAI SPGGRTRMSA SMPGAAAPIE PDERSEDEFD PKDPSLGSPV VAWLASPEAG HISGQVIRAI GEHLQLLKGW HPVASVSNGQ KRWDANKLGA IMAADIFGTR NTGLRLGG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAGGTCTA CTCGACGGCA AGGTCGCTCT GGTGACCGGC GCCGGCCACG GCATCGGCCG CGGTCATGCA CTCGAACTGG CCAAGCACGG CGCGACCGTG ATTGTCAACG ACCTGGGCAC CAGCCTGTCC GGTGAGGGCA CCGGCAAGGT GGCCGACGAG GTGGTGACGG TCATCGAAAG TCGAGGCGGC AAAGCGGTTT CCGACTTCAG CGACGTGGGC GACGAGGAAC AGGTCGACCT CGCGGTGGAG CGGGCCTACT CGCAGCTGGG CCGGTTGGAC ATCGTGGTCA ACAATGCGGG CATCGTGCGC GACAAGGCGA TCTGGAACAT GACCGTCGAC GACTTCGACC TGGTGATGCG GGTGCACGTC CGCGGCAGCT GGCTCACCAG CCGCGCCGTC GCCCGCAAGT GGCGTGCGGA ATCCAAGGAA TCCGGCGCAA AGGTGTACGG CCGCATCATC AACACCACCT CCGGAGCGGG CCTGCACGGC CATTTCGGCC AGACGAACTA CAGCGCGGCC AAGGCCGCGA TCGTCGGACT TACCCAGACC CTGAGCCTGG AACTCGCGTC GATCGGTGCC ACGGTGAATG CCATCAGCCC GGGCGGTCGC ACCCGGATGT CGGCGTCCAT GCCGGGAGCG GCCGCACCCA TCGAGCCCGA CGAGCGGTCC GAGGACGAGT TCGACCCGAA GGATCCGTCT TTGGGCTCGC CGGTGGTGGC CTGGCTGGCC AGCCCCGAGG CCGGACACAT CAGCGGCCAG GTGATCCGCG CCATCGGCGA ACATCTCCAG CTACTCAAGG GCTGGCATCC GGTGGCGTCG GTGTCCAACG GCCAAAAACG CTGGGATGCC AACAAACTCG GCGCGATCAT GGCGGCCGAT ATATTCGGTA CCCGTAACAC CGGCCTGCGC CTGGGAGGTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A