MyavA.01582.a
Short chain dehydrogenase
| CENTER ID: | MyavA.01582.a |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01582.a.A1.GE29136 | Full length( MyavA.01582.a ) | 1 | 301 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01582.a.A1.PS00686 | Full length( MyavA.01582.a ) | 1 | 301 |
Structures
3QLJ
DEPOSITED: 2/2/2011
DETERMINATION: XRay
CLONE: MyavA.01582.a.A1.GE29136
PROTEIN: MyavA.01582.a.A1.PS00686
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_239168 |
| PATRIC ID: | fig|243243.7.peg.593 |
| RefSeq: | YP_879892.1 |
| UniProt: | A0A0H2ZY59 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGVVDGRVVI VTGAGGGIGR AHALAFAAEG ARVVVNDIGV GLDGSPASGG SAAQSVVDEI TAAGGEAVAD GSNVADWDQA AGLIQTAVET FGGLDVLVNN AGIVRDRMIA NTSEEEFDAV IAVHLKGHFA TMRHAAAYWR GLSKAGKAVD GRIINTSSGA GLQGSVGQGN YSAAKAGIAT LTLVGAAEMG RYGVTVNAIA PSARTRMTET VFAEMMATQD QDFDAMAPEN VSPLVVWLGS AEARDVTGKV FEVEGGKIRV AEGWAHGPQI DKGARWDPAE LGPVVADLLG KARPPVPVYG A
NT Sequence
ATGGGAGTGG TTGACGGCCG CGTCGTCATC GTCACCGGAG CGGGCGGCGG TATCGGTCGC GCGCACGCAC TGGCCTTCGC CGCCGAAGGG GCGCGTGTGG TGGTCAACGA CATCGGGGTG GGCCTCGACG GTTCACCGGC CAGCGGCGGC AGCGCCGCCC AGAGCGTGGT GGACGAGATC ACCGCCGCCG GCGGGGAAGC CGTCGCCGAC GGATCCAACG TCGCCGACTG GGACCAGGCC GCCGGTCTGA TCCAGACCGC CGTCGAAACC TTCGGTGGTC TCGACGTTTT GGTGAACAAC GCCGGCATTG TGCGCGACAG GATGATCGCC AACACCAGCG AGGAGGAGTT CGACGCCGTC ATCGCGGTGC ACCTCAAGGG GCACTTCGCC ACCATGCGGC ACGCCGCGGC GTACTGGCGG GGGCTGTCCA AGGCGGGCAA GGCCGTCGAC GGCCGGATCA TCAACACCAG CTCGGGCGCC GGCCTGCAGG GGAGCGTCGG GCAGGGCAAC TACAGCGCCG CCAAGGCCGG TATCGCGACG CTGACGCTGG TCGGCGCGGC GGAGATGGGC CGCTACGGCG TCACCGTCAA CGCGATCGCG CCGTCGGCCC GCACCCGGAT GACCGAGACC GTGTTCGCCG AGATGATGGC AACGCAGGAC CAGGATTTCG ACGCGATGGC CCCGGAAAAC GTGTCGCCCC TGGTGGTTTG GCTGGGCAGT GCGGAAGCCC GCGACGTGAC CGGCAAGGTG TTCGAGGTCG AGGGCGGCAA GATCCGGGTC GCCGAGGGCT GGGCGCACGG CCCGCAGATC GACAAGGGCG CGAGGTGGGA TCCCGCCGAG CTGGGGCCCG TCGTCGCCGA CCTGCTCGGC AAGGCCCGCC CGCCGGTGCC GGTCTACGGC GCC
Details for MyavA.01582.a.A1.GE29136
| HARVESTED ON: | 4/27/2010 |
| SEQUENCED ON: | 5/1/2010 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 60 |
Validated AA Sequence
MIANTSEEEF DAVIAVHLKG HFATMRHAAA YWRGLSKAGK AVDGRIINTS SGAGLQGSVG QGNYSAAKAG IATLTLVGAA EMGRYGVTVN AIAPSARTRM TETVFAEMMA TQDQDFDAMA PENVSPLVVW LGSAEARDVT GKVFEVEGGK IRVAEGWAHG PQIDKGARWD PAELGPVVAD LLGKARPPVP VYGA
Validated NT Sequence
ttattaggcg ccgtagaccg gcaccggcgg gcgggccttg ccgagcaggt cggcgacgac gggccccagc tcggcgggat cccacctcgc gcccttgtcg atctgcgggc cgtgcgccca gccctcggcg acccggatct tgccgccctc gacctcgaac accttgccgg tcacgtcgcg ggcttccgca ctgcccagcc aaaccaccag gggcgacacg ttttccgggg ccatcgcgtc gaaatcctgg tcctgcgttg ccatcatctc ggcgaacacg gtctcggtca tccgggtgcg ggccgacggc gcgatcgcgt tgacggtgac gccgtagcgg cccatctccg ccgcgccgac cagcgtcagc gtcgcgatac cggccttggc ggcgctgtag ttgccctgcc cgacgctccc ctgcaggccg gcgcccgagc tggtgttgat gatccggccg tcgacggcct tgcccgcctt ggacagcccc cgccagtacg ccgcggcgtg ccgcatggtg gcgaagtgcc ccttgaggtg caccgcgatg acggcgtcga actcctcctc gctggtgttg gcgatcatcc tgtcgcgcac aatgccggcg ttgttcacca aaacgtcgag accaccgaag gtttcgacgg cggtctggat cagaccggcg gcctggtccc agtcggcgac gttggatccg tcggcgacgg cttccccgcc ggcggcggtg atctcgtcca ccacgctctg ggcggcgctg ccgccgctgg ccggtgaacc gtcgaggccc accccgatgt cgttgaccac cacacgcgcc ccttcggcgg cgaaggncag tgcgtgngcn cgaccgatac cgccgccc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGVVDGRVV IVTGAGGGIG RAHALAFAAE GARVVVNDIG VGLDGSPASG GSAAQSVVDE ITAAGGEAVA DGSNVADWDQ AAGLIQTAVE TFGGLDVLVN NAGIVRDRMI ANTSEEEFDA VIAVHLKGHF ATMRHAAAYW RGLSKAGKAV DGRIINTSSG AGLQGSVGQG NYSAAKAGIA TLTLVGAAEM GRYGVTVNAI APSARTRMTE TVFAEMMATQ DQDFDAMAPE NVSPLVVWLG SAEARDVTGK VFEVEGGKIR VAEGWAHGPQ IDKGARWDPA ELGPVVADLL GKARPPVPVY GA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggagtggtt gacggccgcg tcgtcatcgt caccggagcg ggcggcggta tcggtcgcgc gcacgcactg gccttcgccg ccgaaggggc gcgtgtggtg gtcaacgaca tcggggtggg cctcgacggt tcaccggcca gcggcggcag cgccgcccag agcgtggtgg acgagatcac cgccgccggc ggggaagccg tcgccgacgg atccaacgtc gccgactggg accaggccgc cggtctgatc cagaccgccg tcgaaacctt cggtggtctc gacgttttgg tgaacaacgc cggcattgtg cgcgacagga tgatcgccaa caccagcgag gaggagttcg acgccgtcat cgcggtgcac ctcaaggggc acttcgccac catgcggcac gccgcggcgt actggcgggg gctgtccaag gcgggcaagg ccgtcgacgg ccggatcatc aacaccagct cgggcgccgg cctgcagggg agcgtcgggc agggcaacta cagcgccgcc aaggccggta tcgcgacgct gacgctggtc ggcgcggcgg agatgggccg ctacggcgtc accgtcaacg cgatcgcgcc gtcggcccgc acccggatga ccgagaccgt gttcgccgag atgatggcaa cgcaggacca ggatttcgac gcgatggccc cggaaaacgt gtcgcccctg gtggtttggc tgggcagtgc ggaagcccgc gacgtgaccg gcaaggtgtt cgaggtcgag ggcggcaaga tccgggtcgc cgagggctgg gcgcacggcc cgcagatcga caagggcgcg aggtgggatc ccgccgagct ggggcccgtc gtcgccgacc tgctcggcaa ggcccgcccg ccggtgccgg tctacggcgc caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MyavA.01582.a.A1.PS00686
| PURIFICATION DATe: | 7/21/2010 |
| CONCENTRATION: | 57.09mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 60 |
Protocol Notes
notes unavailable
Validated AA Sequence
MIANTSEEEF DAVIAVHLKG HFATMRHAAA YWRGLSKAGK AVDGRIINTS SGAGLQGSVG QGNYSAAKAG IATLTLVGAA EMGRYGVTVN AIAPSARTRM TETVFAEMMA TQDQDFDAMA PENVSPLVVW LGSAEARDVT GKVFEVEGGK IRVAEGWAHG PQIDKGARWD PAELGPVVAD LLGKARPPVP VYGA
Validated NT Sequence
ttattaggcg ccgtagaccg gcaccggcgg gcgggccttg ccgagcaggt cggcgacgac gggccccagc tcggcgggat cccacctcgc gcccttgtcg atctgcgggc cgtgcgccca gccctcggcg acccggatct tgccgccctc gacctcgaac accttgccgg tcacgtcgcg ggcttccgca ctgcccagcc aaaccaccag gggcgacacg ttttccgggg ccatcgcgtc gaaatcctgg tcctgcgttg ccatcatctc ggcgaacacg gtctcggtca tccgggtgcg ggccgacggc gcgatcgcgt tgacggtgac gccgtagcgg cccatctccg ccgcgccgac cagcgtcagc gtcgcgatac cggccttggc ggcgctgtag ttgccctgcc cgacgctccc ctgcaggccg gcgcccgagc tggtgttgat gatccggccg tcgacggcct tgcccgcctt ggacagcccc cgccagtacg ccgcggcgtg ccgcatggtg gcgaagtgcc ccttgaggtg caccgcgatg acggcgtcga actcctcctc gctggtgttg gcgatcatcc tgtcgcgcac aatgccggcg ttgttcacca aaacgtcgag accaccgaag gtttcgacgg cggtctggat cagaccggcg gcctggtccc agtcggcgac gttggatccg tcggcgacgg cttccccgcc ggcggcggtg atctcgtcca ccacgctctg ggcggcgctg ccgccgctgg ccggtgaacc gtcgaggccc accccgatgt cgttgaccac cacacgcgcc ccttcggcgg cgaaggncag tgcgtgngcn cgaccgatac cgccgccc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGVVDGRVV IVTGAGGGIG RAHALAFAAE GARVVVNDIG VGLDGSPASG GSAAQSVVDE ITAAGGEAVA DGSNVADWDQ AAGLIQTAVE TFGGLDVLVN NAGIVRDRMI ANTSEEEFDA VIAVHLKGHF ATMRHAAAYW RGLSKAGKAV DGRIINTSSG AGLQGSVGQG NYSAAKAGIA TLTLVGAAEM GRYGVTVNAI APSARTRMTE TVFAEMMATQ DQDFDAMAPE NVSPLVVWLG SAEARDVTGK VFEVEGGKIR VAEGWAHGPQ IDKGARWDPA ELGPVVADLL GKARPPVPVY GA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGAGTGGTT GACGGCCGCG TCGTCATCGT CACCGGAGCG GGCGGCGGTA TCGGTCGCGC GCACGCACTG GCCTTCGCCG CCGAAGGGGC GCGTGTGGTG GTCAACGACA TCGGGGTGGG CCTCGACGGT TCACCGGCCA GCGGCGGCAG CGCCGCCCAG AGCGTGGTGG ACGAGATCAC CGCCGCCGGC GGGGAAGCCG TCGCCGACGG ATCCAACGTC GCCGACTGGG ACCAGGCCGC CGGTCTGATC CAGACCGCCG TCGAAACCTT CGGTGGTCTC GACGTTTTGG TGAACAACGC CGGCATTGTG CGCGACAGGA TGATCGCCAA CACCAGCGAG GAGGAGTTCG ACGCCGTCAT CGCGGTGCAC CTCAAGGGGC ACTTCGCCAC CATGCGGCAC GCCGCGGCGT ACTGGCGGGG GCTGTCCAAG GCGGGCAAGG CCGTCGACGG CCGGATCATC AACACCAGCT CGGGCGCCGG CCTGCAGGGG AGCGTCGGGC AGGGCAACTA CAGCGCCGCC AAGGCCGGTA TCGCGACGCT GACGCTGGTC GGCGCGGCGG AGATGGGCCG CTACGGCGTC ACCGTCAACG CGATCGCGCC GTCGGCCCGC ACCCGGATGA CCGAGACCGT GTTCGCCGAG ATGATGGCAA CGCAGGACCA GGATTTCGAC GCGATGGCCC CGGAAAACGT GTCGCCCCTG GTGGTTTGGC TGGGCAGTGC GGAAGCCCGC GACGTGACCG GCAAGGTGTT CGAGGTCGAG GGCGGCAAGA TCCGGGTCGC CGAGGGCTGG GCGCACGGCC CGCAGATCGA CAAGGGCGCG AGGTGGGATC CCGCCGAGCT GGGGCCCGTC GTCGCCGACC TGCTCGGCAA GGCCCGCCCG CCGGTGCCGG TCTACGGCGC CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA