MyavA.01566.a
Enoyl-CoA hydratase
| CENTER ID: | MyavA.01566.a |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01566.a.A1.GE29135 | Full length( MyavA.01566.a ) | 1 | 249 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01566.a.A1.PS00705 | Full length( MyavA.01566.a ) | 1 | 249 |
Structures
3P85
DEPOSITED: 10/13/2010
DETERMINATION: XRay
CLONE: MyavA.01566.a.A1.GE29135
PROTEIN: MyavA.01566.a.A1.PS00705
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_142767 |
| PATRIC ID: | fig|243243.7.peg.3610 |
| RefSeq: | YP_882866.1 |
| UniProt: | A0A0H2ZUQ2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDEILLSNT EERVRTLTLN RPQARNALSA ALRDRFFGAL ADAETDDDVD VVIITGADPV FCAGLDLKEL GGSSALPDIS PRWPALTKPV IGAINGAAVT GGLELALYCD ILIASENARF ADTHARVGLL PTWGLSVRLP QKVGIGLARR MSLTGDYLSA ADALRAGLVT EVVPHDQLLG AARAVAASIV GNNQNAVRAL LTSYHRIDDA QTSAGLWQEA MAARQFRTSG DDIAANREAV LARGRSQVR
NT Sequence
ATGACCGACG AGATCCTGCT GAGCAACACC GAGGAGCGGG TGCGCACCCT CACCCTCAAC CGGCCCCAGG CCCGCAACGC GCTGTCCGCG GCGTTGCGGG ACCGCTTCTT CGGCGCCCTG GCCGACGCCG AGACGGACGA CGACGTGGAC GTCGTCATCA TCACCGGCGC CGATCCGGTG TTCTGCGCGG GGCTGGATCT CAAGGAGCTG GGCGGCTCGT CGGCGCTGCC GGACATCTCG CCGCGCTGGC CGGCGCTGAC CAAGCCGGTG ATCGGCGCCA TCAACGGCGC CGCGGTCACC GGCGGGCTGG AGCTGGCGCT GTATTGCGAC ATCCTGATCG CCTCGGAGAA CGCCCGCTTC GCCGACACGC ACGCCCGGGT CGGCCTGCTG CCCACCTGGG GGCTGAGCGT GCGGCTGCCG CAGAAGGTCG GCATCGGCCT GGCCCGCCGG ATGAGCCTGA CCGGCGACTA CCTGTCGGCG GCCGACGCGC TGCGGGCCGG GCTGGTCACC GAGGTGGTGC CGCACGACCA GCTGTTGGGC GCCGCCCGGG CCGTGGCGGC CTCGATCGTC GGGAACAACC AGAACGCGGT GCGCGCGCTG CTGACCTCCT ATCACCGCAT CGACGACGCG CAGACCAGCG CGGGGCTGTG GCAGGAGGCC ATGGCGGCCC GGCAGTTCCG GACCAGCGGC GACGACATCG CCGCCAACCG GGAGGCGGTG CTGGCGCGCG GCCGCTCCCA GGTCCGC
Details for MyavA.01566.a.A1.GE29135
| HARVESTED ON: | 4/27/2010 |
| SEQUENCED ON: | 5/1/2010 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDEILLSN TEERVRTLTL NRPQARNALS AALRDRFFGA LADAETDDDV DVVIITGADP VFCAGLDLKE LGGSSALPDI SPRWPALTKP VIGAINGAAV TGGLELALYC DILIASENAR FADTHARVGL LPTWGLSVRL PQKVGIGLAR RMSLTGDYLS AADALRAGLV TEVVPHDQLL GAARAVAASI VGNNQNAVRA LLTSYHRIDD AQTSAGLWQE AMAARQFRTS GDDIAANREA VLARGRSQVR
Validated NT Sequence
ttattagcgg acctgggagc ggccgcgcgc cagcaccgcc tcccggttgg cggcgatgtc gtcgccgctg gtccggaact gccgggccgc catggcctcc tgccacagcc ccgcgctggt ctgcgcgtcg tcgatgcggt gataggaggt cagcagcgcg cgcaccgcgt tctggttgtt cccgacgatc gaggccgcca cggcccgggc ggcgcccaac agctggtcgt gcggcaccac ctcggtgacc agcccggccc gcagcgcgtc ggccgccgac aggtagtcgc cggtcaggct catccggcgg gccaggccga tgccgacctt ctgcggcagc cgcacgctca gcccccaggt gggcagcagg ccgacccggg cgtgcgtgtc ggcgaagcgg gcgttctccg aggcgatcag gatgtcgcaa tacagcgcca gctccagccc gccggtgacc gcggcgccgt tgatggcgcc gatcaccggc ttggtcagcg ccggccagcg cggcgagatg tccggcagcg ccgacgagcc gcccagctcc ttgagatcca gccccgcgca gaacaccgga tcggcgccgg tgatgatgac gacgtccacg tcgtcgtccg tctcggcgtc ggccagggcg ccgaagaagc ggtcccgcaa cgccgcggac agcgcgttgc gggcctgggg ccggttgagg gtgagggtgc gcacccgctc ctcggtgttg ctcagcagga tctcgtcggt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDEILLSN TEERVRTLTL NRPQARNALS AALRDRFFGA LADAETDDDV DVVIITGADP VFCAGLDLKE LGGSSALPDI SPRWPALTKP VIGAINGAAV TGGLELALYC DILIASENAR FADTHARVGL LPTWGLSVRL PQKVGIGLAR RMSLTGDYLS AADALRAGLV TEVVPHDQLL GAARAVAASI VGNNQNAVRA LLTSYHRIDD AQTSAGLWQE AMAARQFRTS GDDIAANREA VLARGRSQVR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgacgag atcctgctga gcaacaccga ggagcgggtg cgcaccctca ccctcaaccg gccccaggcc cgcaacgcgc tgtccgcggc gttgcgggac cgcttcttcg gcgccctggc cgacgccgag acggacgacg acgtggacgt cgtcatcatc accggcgccg atccggtgtt ctgcgcgggg ctggatctca aggagctggg cggctcgtcg gcgctgccgg acatctcgcc gcgctggccg gcgctgacca agccggtgat cggcgccatc aacggcgccg cggtcaccgg cgggctggag ctggcgctgt attgcgacat cctgatcgcc tcggagaacg cccgcttcgc cgacacgcac gcccgggtcg gcctgctgcc cacctggggg ctgagcgtgc ggctgccgca gaaggtcggc atcggcctgg cccgccggat gagcctgacc ggcgactacc tgtcggcggc cgacgcgctg cgggccgggc tggtcaccga ggtggtgccg cacgaccagc tgttgggcgc cgcccgggcc gtggcggcct cgatcgtcgg gaacaaccag aacgcggtgc gcgcgctgct gacctcctat caccgcatcg acgacgcgca gaccagcgcg gggctgtggc aggaggccat ggcggcccgg cagttccgga ccagcggcga cgacatcgcc gccaaccggg aggcggtgct ggcgcgcggc cgctcccagg tccgcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MyavA.01566.a.A1.PS00705
| PURIFICATION DATe: | 8/9/2010 |
| CONCENTRATION: | 75.69mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDEILLSN TEERVRTLTL NRPQARNALS AALRDRFFGA LADAETDDDV DVVIITGADP VFCAGLDLKE LGGSSALPDI SPRWPALTKP VIGAINGAAV TGGLELALYC DILIASENAR FADTHARVGL LPTWGLSVRL PQKVGIGLAR RMSLTGDYLS AADALRAGLV TEVVPHDQLL GAARAVAASI VGNNQNAVRA LLTSYHRIDD AQTSAGLWQE AMAARQFRTS GDDIAANREA VLARGRSQVR
Validated NT Sequence
ttattagcgg acctgggagc ggccgcgcgc cagcaccgcc tcccggttgg cggcgatgtc gtcgccgctg gtccggaact gccgggccgc catggcctcc tgccacagcc ccgcgctggt ctgcgcgtcg tcgatgcggt gataggaggt cagcagcgcg cgcaccgcgt tctggttgtt cccgacgatc gaggccgcca cggcccgggc ggcgcccaac agctggtcgt gcggcaccac ctcggtgacc agcccggccc gcagcgcgtc ggccgccgac aggtagtcgc cggtcaggct catccggcgg gccaggccga tgccgacctt ctgcggcagc cgcacgctca gcccccaggt gggcagcagg ccgacccggg cgtgcgtgtc ggcgaagcgg gcgttctccg aggcgatcag gatgtcgcaa tacagcgcca gctccagccc gccggtgacc gcggcgccgt tgatggcgcc gatcaccggc ttggtcagcg ccggccagcg cggcgagatg tccggcagcg ccgacgagcc gcccagctcc ttgagatcca gccccgcgca gaacaccgga tcggcgccgg tgatgatgac gacgtccacg tcgtcgtccg tctcggcgtc ggccagggcg ccgaagaagc ggtcccgcaa cgccgcggac agcgcgttgc gggcctgggg ccggttgagg gtgagggtgc gcacccgctc ctcggtgttg ctcagcagga tctcgtcggt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDEILLSN TEERVRTLTL NRPQARNALS AALRDRFFGA LADAETDDDV DVVIITGADP VFCAGLDLKE LGGSSALPDI SPRWPALTKP VIGAINGAAV TGGLELALYC DILIASENAR FADTHARVGL LPTWGLSVRL PQKVGIGLAR RMSLTGDYLS AADALRAGLV TEVVPHDQLL GAARAVAASI VGNNQNAVRA LLTSYHRIDD AQTSAGLWQE AMAARQFRTS GDDIAANREA VLARGRSQVR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGACGAG ATCCTGCTGA GCAACACCGA GGAGCGGGTG CGCACCCTCA CCCTCAACCG GCCCCAGGCC CGCAACGCGC TGTCCGCGGC GTTGCGGGAC CGCTTCTTCG GCGCCCTGGC CGACGCCGAG ACGGACGACG ACGTGGACGT CGTCATCATC ACCGGCGCCG ATCCGGTGTT CTGCGCGGGG CTGGATCTCA AGGAGCTGGG CGGCTCGTCG GCGCTGCCGG ACATCTCGCC GCGCTGGCCG GCGCTGACCA AGCCGGTGAT CGGCGCCATC AACGGCGCCG CGGTCACCGG CGGGCTGGAG CTGGCGCTGT ATTGCGACAT CCTGATCGCC TCGGAGAACG CCCGCTTCGC CGACACGCAC GCCCGGGTCG GCCTGCTGCC CACCTGGGGG CTGAGCGTGC GGCTGCCGCA GAAGGTCGGC ATCGGCCTGG CCCGCCGGAT GAGCCTGACC GGCGACTACC TGTCGGCGGC CGACGCGCTG CGGGCCGGGC TGGTCACCGA GGTGGTGCCG CACGACCAGC TGTTGGGCGC CGCCCGGGCC GTGGCGGCCT CGATCGTCGG GAACAACCAG AACGCGGTGC GCGCGCTGCT GACCTCCTAT CACCGCATCG ACGACGCGCA GACCAGCGCG GGGCTGTGGC AGGAGGCCAT GGCGGCCCGG CAGTTCCGGA CCAGCGGCGA CGACATCGCC GCCAACCGGG AGGCGGTGCT GGCGCGCGGC CGCTCCCAGG TCCGCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA