MyavA.01549.a

Pyruvate phosphate dikinase

CENTER ID: MyavA.01549.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01549.a.A1.GE29132 Full length( MyavA.01549.a ) 1 521
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.1232
RefSeq: YP_880504.1
UniProt: A0A0H2ZWU3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVLLDGTSSH PRELLGNKGH GIEVMRRHHL PVPPAFCLTT AVGLRYLADP AATMDAVWDD VLDRIGWLQA QTSRAFGRGP HPLLVSVRSG ATQSMPGMMD TLLNLGINDS VEQALAAEHG PAFARDTHRR FREMYRRIVG VERPVPSDPY TQLRAGIEAV FASWNSPRAR AYRAHYGFAD RHGTAVVVQA MVFGNHGPNS GAGAFFSRNP ITGDDEPFGE WLPGGQGDDV VSGSVDVEPI AALRDEQPAV YDQLMAAART LERLDRDVQE IEFTVEDGAL WLLQTRAAER SAQAAVRSAL QLRREGLIDD AETLRRVTPA HVRSLLMPAL QPEIRLTAPL LAKGLPACPG VVSGTAYADV DEALHAAERG EPVILVRDHT RPEDVSGMLA ARGIVTEVGG AASHAAVVSR ELGRVAVVGC GAGVAAMLAG RRITVDGAEG EVREGELSLS AWSENDTPEL RELAEIARLI SPLRAHSGGD HPRLDDDSES AVRAALAAGQ TDVVSPTPLL VMLTALRQGA P
NT Sequence
GTGGTGTTGC TGGACGGCAC ATCGAGCCAC CCCCGCGAGC TGCTGGGTAA CAAGGGCCAC GGCATCGAGG TGATGCGCCG CCACCACCTG CCGGTGCCGC CCGCGTTCTG CCTCACCACC GCGGTGGGCC TGCGGTATCT GGCCGACCCC GCGGCGACGA TGGACGCCGT CTGGGACGAC GTGCTGGACC GGATCGGCTG GCTGCAGGCG CAAACCTCGC GCGCGTTCGG GCGGGGCCCG CACCCGCTGC TGGTCAGCGT GCGCTCCGGG GCCACCCAGT CGATGCCCGG GATGATGGAC ACCCTGTTGA ACCTCGGCAT CAACGACTCC GTGGAGCAGG CGCTGGCCGC CGAGCACGGC CCGGCGTTCG CCCGCGACAC CCACCGGCGG TTCCGCGAGA TGTACCGGCG CATCGTGGGT GTCGAGCGGC CGGTGCCGTC CGACCCGTAC ACGCAATTGC GCGCCGGCAT CGAGGCGGTG TTCGCGTCGT GGAACTCCCC GCGCGCCCGC GCCTACCGCG CCCATTACGG TTTCGCCGAC CGGCACGGCA CCGCCGTCGT CGTGCAGGCC ATGGTGTTCG GCAATCACGG CCCCAATTCC GGTGCGGGAG CATTCTTTTC GCGCAACCCG ATCACCGGGG ACGACGAGCC GTTCGGCGAG TGGCTGCCCG GGGGCCAGGG CGACGACGTG GTGTCGGGAT CGGTCGACGT GGAACCGATC GCCGCGCTGC GCGACGAGCA GCCGGCCGTC TACGACCAGC TGATGGCCGC CGCCCGGACG CTGGAGCGGT TGGACCGTGA CGTCCAGGAG ATCGAGTTCA CCGTCGAGGA CGGCGCCCTG TGGTTGCTGC AGACCAGGGC GGCGGAACGC TCGGCGCAGG CGGCGGTGCG CTCGGCGCTG CAGCTGCGAC GCGAGGGGCT GATCGACGAC GCCGAGACGC TGCGGCGGGT CACCCCGGCG CACGTGCGGT CGCTGCTGAT GCCCGCCCTG CAACCCGAAA TACGTTTGAC GGCACCACTT CTGGCCAAGG GCCTACCTGC CTGCCCCGGC GTGGTATCCG GCACGGCCTA CGCCGACGTC GACGAGGCGC TGCACGCCGC CGAGCGGGGC GAACCGGTGA TCCTGGTGCG CGACCACACC CGGCCCGAAG ACGTGTCGGG CATGCTGGCC GCGCGGGGCA TCGTCACCGA GGTGGGCGGC GCGGCCAGCC ATGCGGCGGT GGTCAGCCGG GAACTGGGCC GGGTGGCCGT GGTGGGTTGC GGCGCCGGGG TGGCCGCGAT GCTGGCGGGC CGGCGGATCA CCGTCGACGG CGCCGAAGGC GAAGTGCGCG AAGGCGAATT GTCCCTGTCC GCGTGGTCCG AGAACGACAC GCCGGAGCTG CGGGAGCTGG CCGAGATCGC GCGGCTGATC AGCCCGCTGC GCGCCCATTC CGGCGGCGAC CATCCGCGGC TGGACGACGA CTCCGAGAGC GCCGTGCGCG CGGCCCTGGC CGCCGGGCAG ACCGACGTGG TCTCGCCCAC CCCGCTGCTG GTCATGCTGA CCGCGCTGCG CCAGGGCGCG CCG
Details for MyavA.01549.a.A1.GE29132
HARVESTED ON: 4/27/2010
SEQUENCED ON: 5/1/2010
EXPECTED MW: 58kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 90
Validated AA Sequence
MVVLLDGTSS HPRXLLGNKG HGIEVMRRHH LPVPPAFCLT TAVGLRYLAD PAATMDAVWD DVLDRIGWLQ AQTSRAFGRG PHPLLVSVRS GATQSMPGMM DTLLNLGIND SVEQALAAEH GPAFARDTHR RFREMYRRIV GVERPVPSDP YTQLRAGIEA VFASWNSPRA RAYRAHYGFA DRHGTAVVVQ AMVFGNHGPN SGAGAFFSRN PITGDDEPFG EWLPGGXGDD VVSGSVDVXP IAALRDEQPX VYDQLMAAAR TLERLDRDVQ EIEFTVEDGA LWLLQTRAAE RSAQAAVRSA LQLRREGLID DAETLRRVTP AHVRSLLMPA LQPEIRLTAP LLAKGLPACP GVVSGTAYAD VDEALHAAER GEPVILVRDH TRPEDVSGML AARGIVTEVG GAASHAAVVS RELGRVAVVG CGAGVAAMLA GRRITVDGAE GEVREGELSL SAWSENDTPE LRELAEIARX ISPLRAHSGG DHPRLDDDSE SA
Validated NT Sequence
cggcgctctc ggagtcgtcg tccagccgcg gatggtcgcc gccggaatgg gcgcgcagcg ggctgatcan ccgcgcgatc tcggccagct cccgcagctc cggcgtgtcg ttctcggacc acgcggacag ggacaattcg ccttcgcgca cttcgccttc ggcgccgtcg acggtgatcc gccggcccgc cagcatcgcg gccaccccgg cgccgcaacc caccacggcc acccggccca gttcccggct gaccaccgcc gcatggctgg ccgcgccgcc cacctcggtg acgatgcccc gcgcggccag catgcccgac acgtcttcgg gccgggtgtg gtcgcgcacc aggatcaccg gttcgccccg ctcggcggcg tgcagcgcct cgtcgacgtc ggcgtaggcc gtgccggata ccacgccggg gcaggcaggt aggcccttgg ccagaagtgg tgccgtcaaa cgtatttcgg gttgcagggc gggcatcagc agcgaccgca cgtgcgccgg ggtgacccgc cgcagcgtct cggcgtcgtc gatcagcccc tcgcgtcgca gctgcagcgc cgagcgcacc gccgcctgcg ccgagcgttc cgccgccctg gtctgcagca accacagggc gccgtcctcg acggtgaact cgatctcctg gacgtcacgg tccaaccgct ccagcgtccg ggcggcggcc atcagctggt cgtagacggn cggctgctcg tcgcgcagcg cggcgatcgg ttncacgtcg accgatcccg acaccacgtc gtcgccntgg cccccgggca gccactcgcc gaacggctcg tcgtccccgg tgatcgggtt gcgcgaaaag aatgctcccg caccggaatt ggggccgtga ttgccgaaca ccatggcctg cacgacgacg gcggtgccgt gccggtcggc gaaaccgtaa tgggcgcggt aggcgcgggc gcgcggggag ttccacgacg cgaacaccgc ctcgatgccg gcgcgcaatt gcgtgtacgg gtcggacggc accggccgct cgacacccac gatgcgccgg tacatctcgc ggaaccgccg gtgggtgtcg cgggcgaacg ccgggccgtg ctcggcggcc agcgcctgct ccacggagtc gttgatgccg aggttcaaca gggtgtccat catcccgggc atcgactggg tggccccgga gcgcacgctg accagcagcg ggtgcgggcc ccgcccgaac gcgcgcgagg tttgcgcctg cagccagccg atccggtcca gcacgtcgtc ccagacggcg tccatcgtcg ccgcggggtc ggccagatac cgcaggccca ccgcggtggt gaggcagaac gcgggcggca ccggcaggtg gtggcggcgc atcacctcga tgccgtggcc cttgttaccc agcagctngc gggggtggct cgatgtgccg tccagcaaca ccaccatcga accaggaccc tgggtctgag cttccagggt a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVVLLDGTS SHPRELLGNK GHGIEVMRRH HLPVPPAFCL TTAVGLRYLA DPAATMDAVW DDVLDRIGWL QAQTSRAFGR GPHPLLVSVR SGATQSMPGM MDTLLNLGIN DSVEQALAAE HGPAFARDTH RRFREMYRRI VGVERPVPSD PYTQLRAGIE AVFASWNSPR ARAYRAHYGF ADRHGTAVVV QAMVFGNHGP NSGAGAFFSR NPITGDDEPF GEWLPGGQGD DVVSGSVDVE PIAALRDEQP AVYDQLMAAA RTLERLDRDV QEIEFTVEDG ALWLLQTRAA ERSAQAAVRS ALQLRREGLI DDAETLRRVT PAHVRSLLMP ALQPEIRLTA PLLAKGLPAC PGVVSGTAYA DVDEALHAAE RGEPVILVRD HTRPEDVSGM LAARGIVTEV GGAASHAAVV SRELGRVAVV GCGAGVAAML AGRRITVDGA EGEVREGELS LSAWSENDTP ELRELAEIAR LISPLRAHSG GDHPRLDDDS ESAVRAALAA GQTDVVSPTP LLVMLTALRQ GAP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggtgttg ctggacggca catcgagcca cccccgcgag ctgctgggta acaagggcca cggcatcgag gtgatgcgcc gccaccacct gccggtgccg cccgcgttct gcctcaccac cgcggtgggc ctgcggtatc tggccgaccc cgcggcgacg atggacgccg tctgggacga cgtgctggac cggatcggct ggctgcaggc gcaaacctcg cgcgcgttcg ggcggggccc gcacccgctg ctggtcagcg tgcgctccgg ggccacccag tcgatgcccg ggatgatgga caccctgttg aacctcggca tcaacgactc cgtggagcag gcgctggccg ccgagcacgg cccggcgttc gcccgcgaca cccaccggcg gttccgcgag atgtaccggc gcatcgtggg tgtcgagcgg ccggtgccgt ccgacccgta cacgcaattg cgcgccggca tcgaggcggt gttcgcgtcg tggaactccc cgcgcgcccg cgcctaccgc gcccattacg gtttcgccga ccggcacggc accgccgtcg tcgtgcaggc catggtgttc ggcaatcacg gccccaattc cggtgcggga gcattctttt cgcgcaaccc gatcaccggg gacgacgagc cgttcggcga gtggctgccc gggggccagg gcgacgacgt ggtgtcggga tcggtcgacg tggaaccgat cgccgcgctg cgcgacgagc agccggccgt ctacgaccag ctgatggccg ccgcccggac gctggagcgg ttggaccgtg acgtccagga gatcgagttc accgtcgagg acggcgccct gtggttgctg cagaccaggg cggcggaacg ctcggcgcag gcggcggtgc gctcggcgct gcagctgcga cgcgaggggc tgatcgacga cgccgagacg ctgcggcggg tcaccccggc gcacgtgcgg tcgctgctga tgcccgccct gcaacccgaa atacgtttga cggcaccact tctggccaag ggcctacctg cctgccccgg cgtggtatcc ggcacggcct acgccgacgt cgacgaggcg ctgcacgccg ccgagcgggg cgaaccggtg atcctggtgc gcgaccacac ccggcccgaa gacgtgtcgg gcatgctggc cgcgcggggc atcgtcaccg aggtgggcgg cgcggccagc catgcggcgg tggtcagccg ggaactgggc cgggtggccg tggtgggttg cggcgccggg gtggccgcga tgctggcggg ccggcggatc accgtcgacg gcgccgaagg cgaagtgcgc gaaggcgaat tgtccctgtc cgcgtggtcc gagaacgaca cgccggagct gcgggagctg gccgagatcg cgcggctgat cagcccgctg cgcgcccatt ccggcggcga ccatccgcgg ctggacgacg actccgagag cgccgtgcgc gcggccctgg ccgccgggca gaccgacgtg gtctcgccca ccccgctgct ggtcatgctg accgcgctgc gccagggcgc gccgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa