MyavA.01548.a

Methionine aminopeptidase

CENTER ID:	MyavA.01548.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01548.a.A2.GE29131	Mature protein( MyavA.01548.a )		40	266

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126721
PATRIC ID:	fig\|243243.7.peg.4353
RefSeq:	YP_883567.1
UniProt:	A0A0H2ZV58

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNALARLRGR KVVPQRSDGE LDAMAAAGAV VAAALQAVRA AAVAGVSTLS LDQIAESVIR DAGAVPSFLG YHGYPASICA SVNDRVVHGI PSGAEVLAPG DLVSMDCGAV LDGWHGDAAI TFGVGALDPA DEALRQATRE SLEAGIAAMV PGNRLTDVSH AIELGTRAAE TRHGRAFGIV EGYGGHGIGR QMHMDPFLPN EGSPGRGPLL APGSVLAIEP MLTLGTGKTV VLDDQWTVIT TDGSRAAHWE HTVAVTDAGP RILTLG

NT Sequence

ATGAACGCGC TGGCGCGGCT GCGCGGCCGC AAGGTGGTGC CGCAGCGCAG CGACGGCGAA CTCGACGCGA TGGCCGCCGC GGGCGCGGTG GTGGCCGCCG CGCTGCAGGC GGTCCGCGCG GCCGCGGTCG CCGGGGTGTC GACGCTGAGC CTGGACCAGA TCGCCGAGTC GGTGATCCGG GATGCCGGCG CGGTGCCGTC GTTCCTGGGC TACCACGGTT ACCCGGCCTC CATCTGCGCC TCCGTCAACG ACCGGGTGGT GCACGGAATC CCTTCGGGCG CAGAGGTTTT GGCGCCCGGC GACCTGGTGT CGATGGACTG CGGCGCGGTG CTGGACGGCT GGCACGGGGA CGCGGCGATC ACCTTCGGGG TGGGCGCCCT GGACCCGGCC GACGAGGCGC TGCGCCAGGC CACCCGGGAG TCGCTGGAAG CCGGGATCGC GGCGATGGTG CCCGGGAACC GGTTGACCGA CGTCTCGCAC GCCATCGAGC TGGGCACCCG CGCCGCCGAG ACCCGGCACG GACGGGCGTT CGGCATCGTC GAGGGCTACG GCGGGCACGG CATCGGCCGG CAGATGCACA TGGACCCGTT CCTGCCCAAC GAGGGCTCGC CGGGCCGCGG TCCGCTGCTG GCCCCCGGCT CGGTGCTGGC CATCGAGCCG ATGCTGACCC TGGGCACCGG CAAGACCGTG GTGCTCGACG ACCAGTGGAC GGTCATCACC ACCGACGGCT CGCGTGCGGC ACACTGGGAG CACACCGTTG CCGTCACCGA CGCCGGGCCG CGCATCCTGA CGCTCGGG

Details for MyavA.01548.a.A2.GE29131

HARVESTED ON:	4/27/2010
SEQUENCED ON:	5/1/2010
EXPECTED MW:	25kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAAAVAGVS TLSLDQIAES VIRDAGAVPS FLGYHGYPAS ICASVNDRVV HGIPSGAEVL APGDLVSMDC GAVLDGWHGD AAITFGVGAL DPADEALRQA TRESLEAGIA AMVPGNRLTD VSHAIELGTR AAETRHGRAF GIVEGYGGHG IGRQMHMDPF LPNEGSPGRG PLLAPGSVLA IEPMLTLGTG KTVVLDDQWT VITTDGSRAA HWEHTVAVTD AGPRILTLG

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgg ccgcggtcgc cggggtgtcg acgctgagcc tggaccagat cgccgagtcg gtgatccggg atgccggcgc ggtgccgtcg ttcctgggct accacggtta cccggcctcc atctgcgcct ccgtcaacga ccgggtggtg cacggaatcc cttcgggcgc agaggttttg gcgcccggcg acctggtgtc gatggactgc ggcgcggtgc tggacggctg gcacggggac gcggcgatca ccttcggggt gggcgccctg gacccggccg acgaggcgct gcgccaggcc acccgggagt cgctggaagc cgggatcgcg gcgatggtgc ccgggaaccg gttgaccgac gtctcgcacg ccatcgagct gggcacccgc gccgccgaga cccggcacgg acgggcgttc ggcatcgtcg agggctacgg cgggcacggc atcggccggc agatgcacat ggacccgttc ctgcccaacg agggctcgcc gggccgcggt ccgctgctgg cccccggctc ggtgctggcc atcgagccga tgctgaccct gggcaccggc aagaccgtgg tgctcgacga ccagtggacg gtcatcacca ccgacggctc gcgtgcggca cactgggagc acaccgttgc cgtcaccgac gccgggccgc gcatcctgac gctcgggtaa taa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggccgcg gtcgccgggg tgtcgacgct gagcctggac cagatcgccg agtcggtgat ccgggatgcc ggcgcggtgc cgtcgttcct gggctaccac ggttacccgg cctccatctg cgcctccgtc aacgaccggg tggtgcacgg aatcccttcg ggcgcagagg ttttggcgcc cggcgacctg gtgtcgatgg actgcggcgc ggtgctggac ggctggcacg gggacgcggc gatcaccttc ggggtgggcg ccctggaccc ggccgacgag gcgctgcgcc aggccacccg ggagtcgctg gaagccggga tcgcggcgat ggtgcccggg aaccggttga ccgacgtctc gcacgccatc gagctgggca cccgcgccgc cgagacccgg cacggacggg cgttcggcat cgtcgagggc tacggcgggc acggcatcgg ccggcagatg cacatggacc cgttcctgcc caacgagggc tcgccgggcc gcggtccgct gctggccccc ggctcggtgc tggccatcga gccgatgctg accctgggca ccggcaagac cgtggtgctc gacgaccagt ggacggtcat caccaccgac ggctcgcgtg cggcacactg ggagcacacc gttgccgtca ccgacgccgg gccgcgcatc ctgacgctcg ggaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa