MyavA.01530.c

Enoyl-CoA isomerase

CENTER ID:	MyavA.01530.c
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01530.c.A1.GE29127	Full length( MyavA.01530.c )		1	252

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_153107
PATRIC ID:	fig\|243243.7.peg.1843
RefSeq:	YP_881104.1
UniProt:	A0A0H3A2K5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNPGISIERE GGILRLTLDR PDKLNAVDTP MLNELLGRLD ESADDASVRA VLLTGAGRAF CSGGDLTGGD TEGAAQAAND VVRAITELPK PVVAGVHGPA VGVGCALALA CDLVVATRAA YFQLAFSKLG LMLDGGASVL LPAAIGRARA ARVAMTAEKI TAATAFEWGL ISYVVDDDAY ETDLAELTST LANGPTHSYR WIKRALSAAT LSDLESVQSL EADGQRLLVR TSDFAEGVRA FRERRPTEFR GE

NT Sequence

GTGAATCCCG GTATCTCGAT CGAGCGTGAG GGCGGGATCC TGCGACTGAC GTTGGACCGC CCCGACAAGC TGAACGCCGT CGACACGCCG ATGCTCAACG AACTCCTGGG GCGCCTCGAC GAGTCGGCGG ATGACGCATC GGTGCGCGCC GTGCTGCTGA CCGGCGCCGG GCGCGCGTTC TGTTCCGGCG GCGACCTGAC CGGAGGCGAC ACAGAGGGCG CTGCACAGGC GGCCAACGAC GTCGTACGGG CGATCACGGA ACTGCCCAAA CCCGTGGTGG CGGGTGTGCA TGGACCGGCG GTCGGCGTCG GCTGCGCGCT CGCGCTGGCC TGTGACTTGG TGGTTGCCAC GAGAGCAGCT TATTTCCAAC TGGCTTTCAG TAAGCTCGGG CTGATGCTCG ATGGCGGAGC GTCGGTGCTG TTGCCTGCCG CAATAGGGCG GGCGCGGGCG GCGCGGGTGG CGATGACAGC CGAGAAGATC ACGGCAGCAA CCGCATTCGA GTGGGGCTTG ATCTCGTATG TCGTCGACGA CGACGCGTAT GAGACTGACC TGGCAGAGTT GACGAGCACG TTGGCGAACG GTCCGACTCA CTCCTATCGA TGGATCAAGC GGGCACTGTC GGCCGCAACC TTGTCCGACC TCGAGTCGGT GCAATCTCTC GAGGCCGACG GTCAACGACT ATTGGTCAGA ACCTCGGACT TCGCCGAAGG CGTACGCGCG TTCCGCGAGC GGCGTCCTAC TGAATTTCGA GGTGAG

Details for MyavA.01530.c.A1.GE29127

HARVESTED ON:	4/27/2010
SEQUENCED ON:	5/1/2010
EXPECTED MW:	28kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVNPGISIE REGGILRLTL DRPDKLNAVD TPMLNELLGR LDESADDASV RAVLLTGAGR AFCSGGDLTG GDTEGAAQAA NDVVRAITEL PKPVVAGVHG PAVGVGCALA LACDLVVATR AAYFQLAFSK LGLMLDGGAS VLLPAAIGRA RAARVAMTAE KITAATAFEW GLISYVVDDD AYETDLAELT STLANGPTHS YRWIKRALSA ATLSDLESVQ SLEADGQRLL VRTSDFAEGV RAFRERRPTE FRGE

Validated NT Sequence

ttattactca cctcgaaatt cagtaggacg ccgctcgcgg aacgcgcgta cgccttcggc gaagtccgag gttctgacca atagtcgttg accgtcggcc tcgagagatt gcaccgactc gaggtcggac aaggttgcgg ccgacagtgc ccgcttgatc catcgatagg agtgagtcgg accgttcgcc aacgtgctcg tcaactctgc caggtcagtc tcatacgcgt cgtcgtcgac gacatacgag atcaagcccc actcgaatgc ggttgctgcc gtgatcttct cggctgtcat cgccacccgc gccgcccgcg cccgccctat tgcggcaggc aacagcaccg acgctccgcc atcgagcatc agcccgagct tactgaaagc cagttggaaa taagctgctc tcgtggcaac caccaagtca caggccagcg cgagcgcgca gccgacgccg accgccggtc catgcacacc cgccaccacg ggtttgggca gttccgtgat cgcccgtacg acgtcgttgg ccgcctgtgc agcgccctct gtgtcgcctc cggtcaggtc gccgccggaa cagaacgcgc gcccggcgcc ggtcagcagc acggcgcgca ccgatgcgtc atccgccgac tcgtcgaggc gccccaggag ttcgttgagc atcggcgtgt cgacggcgtt cagcttgtcg gggcggtcca acgtcagtcg caggatcccg ccctcacgct cgatcgagat accgggattc accatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaatccc ggtatctcga tcgagcgtga gggcgggatc ctgcgactga cgttggaccg ccccgacaag ctgaacgccg tcgacacgcc gatgctcaac gaactcctgg ggcgcctcga cgagtcggcg gatgacgcat cggtgcgcgc cgtgctgctg accggcgccg ggcgcgcgtt ctgttccggc ggcgacctga ccggaggcga cacagagggc gctgcacagg cggccaacga cgtcgtacgg gcgatcacgg aactgcccaa acccgtggtg gcgggtgtgc atggaccggc ggtcggcgtc ggctgcgcgc tcgcgctggc ctgtgacttg gtggttgcca cgagagcagc ttatttccaa ctggctttca gtaagctcgg gctgatgctc gatggcggag cgtcggtgct gttgcctgcc gcaatagggc gggcgcgggc ggcgcgggtg gcgatgacag ccgagaagat cacggcagca accgcattcg agtggggctt gatctcgtat gtcgtcgacg acgacgcgta tgagactgac ctggcagagt tgacgagcac gttggcgaac ggtccgactc actcctatcg atggatcaag cgggcactgt cggccgcaac cttgtccgac ctcgagtcgg tgcaatctct cgaggccgac ggtcaacgac tattggtcag aacctcggac ttcgccgaag gcgtacgcgc gttccgcgag cggcgtccta ctgaatttcg aggtgagaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa