MyavA.01530.a
Enoyl-CoA hydratase
| CENTER ID: | MyavA.01530.a |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01530.a.A1.GE29125 | Full length( MyavA.01530.a ) | 1 | 263 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01530.a.A1.PW29030 | Full length( MyavA.01530.a ) | 1 | 263 | ||
| MyavA.01530.a.A1.PW29874 | Full length( MyavA.01530.a ) | 1 | 263 |
Structures
3OC7
DEPOSITED: 8/9/2010
DETERMINATION: XRay
CLONE: MyavA.01530.a.A1.GE29125
PROTEIN: MyavA.01530.a.A1.PW29874
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_159313 |
| PATRIC ID: | fig|243243.7.peg.1060 |
| RefSeq: | YP_880340.1 |
| UniProt: | A0A0H2ZTL3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDALVDYAGP AATGGPVARL TLNSPHNRNA LSTALVSQLH QGLRDASSDP AVRVVVLAHT GGTFCAGADL SEAGSGGSPS SAYDMAVERA REMAALMRAI VESRLPVIAA IDGHVRAGGF GLVGACDIAV AGPRSSFALT EARIGVAPAI ISLTLLPKLS ARAAARYYLT GEKFDARRAE EIGLITMAAE DLDAAIDQLV TDVGRGSPQG LAASKALTTA AVLERFDRDA ERLAEESARL FVSDEAREGM LAFLEKRSPN WTS
NT Sequence
ATGGACGCCC TGGTCGACTA CGCCGGCCCC GCCGCCACCG GCGGTCCGGT GGCGCGGCTG ACGCTGAACT CACCGCACAA CCGCAACGCG CTGTCCACCG CGCTGGTCAG CCAGTTGCAT CAGGGGCTGC GCGACGCCTC GTCGGATCCG GCGGTGCGCG TGGTGGTGCT GGCCCATACC GGCGGCACCT TCTGCGCGGG TGCGGATCTC AGCGAGGCCG GGTCCGGCGG GTCGCCCTCG TCGGCCTACG ACATGGCCGT CGAGCGGGCC CGGGAGATGG CCGCCCTGAT GCGCGCCATC GTCGAATCCC GACTGCCGGT GATCGCCGCG ATCGACGGCC ACGTCCGGGC CGGCGGGTTC GGCTTGGTGG GCGCCTGCGA CATCGCGGTC GCCGGCCCGC GCAGCAGCTT CGCGCTGACC GAGGCGCGGA TCGGCGTCGC CCCGGCCATC ATCTCGCTGA CGCTGCTGCC GAAGCTGTCG GCGCGCGCGG CGGCGCGCTA CTACCTGACC GGCGAGAAGT TCGACGCCCG GCGGGCCGAG GAGATCGGGT TGATCACCAT GGCCGCCGAG GACCTAGACG CCGCGATCGA CCAACTCGTC ACCGACGTGG GCCGCGGTTC GCCGCAGGGC CTCGCGGCGT CCAAGGCACT GACCACCGCC GCGGTGCTGG AGCGCTTCGA CCGGGACGCC GAGCGGCTGG CTGAGGAATC CGCCCGGCTG TTCGTGTCCG ACGAAGCCCG CGAAGGCATG CTGGCGTTCC TGGAGAAGCG GTCACCCAAC TGGACGAGT
Details for MyavA.01530.a.A1.GE29125
| HARVESTED ON: | 4/27/2010 |
| SEQUENCED ON: | 5/1/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDALVDYAG PAATGGPVAR LTLNSPHNRN ALSTALVSQL HQGLRDASSD PAVRVVVLAH TGGTFCAGAD LSEAGSGGSP SSAYDMAVER AREMAALMRA IVESRLPVIA AIDGHVRAGG FGLVGACDIA VAGPRSSFAL TEARIGVAPA IISLTLLPKL SARAAARYYL TGEKFDARRA EEIGLITMAA EDLDAAIDQL VTDVGRGSPQ GLAASKALTT AAVLERFDRD AERLAEESAR LFVSDEAREG MLAFLEKRSP NWTS
Validated NT Sequence
ttattaactc gtccagttgg gtgaccgctt ctccaggaac gccagcatgc cttcgcgggc ttcgtcggac acgaacagcc gggcggattc ctcagccagc cgctcggcgt cccggtcgaa gcgctccagc accgcggcgg tggtcagtgc cttggacgcc gcgaggccct gcggcgaacc gcggcccacg tcggtgacga gttggtcgat cgcggcgtct aggtcctcgg cggccatggt gatcaacccg atctcctcgg cccgccgggc gtcgaacttc tcgccggtca ggtagtagcg cgccgccgcg cgcgccgaca gcttcggcag cagcgtcagc gagatgatgg ccggggcgac gccgatccgc gcctcggtca gcgcgaagct gctgcgcggg ccggcgaccg cgatgtcgca ggcgcccacc aagccgaacc cgccggcccg gacgtggccg tcgatcgcgg cgatcaccgg cagtcgggat tcgacgatgg cgcgcatcag ggcggccatc tcccgggccc gctcgacggc catgtcgtag gccgacgagg gcgacccgcc ggacccggcc tcgctgagat ccgcacccgc gcagaaggtg ccgccggtat gggccagcac caccacgcgc accgccggat ccgacgaggc gtcgcgcagc ccctgatgca actggctgac cagcgcggtg gacagcgcgt tgcggttgtg cggtgagttc agcgtcagcc gcgccaccgg accgccggtg gcggcggggc cggcgtagtc gaccagggcg tccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDALVDYAG PAATGGPVAR LTLNSPHNRN ALSTALVSQL HQGLRDASSD PAVRVVVLAH TGGTFCAGAD LSEAGSGGSP SSAYDMAVER AREMAALMRA IVESRLPVIA AIDGHVRAGG FGLVGACDIA VAGPRSSFAL TEARIGVAPA IISLTLLPKL SARAAARYYL TGEKFDARRA EEIGLITMAA EDLDAAIDQL VTDVGRGSPQ GLAASKALTT AAVLERFDRD AERLAEESAR LFVSDEAREG MLAFLEKRSP NWTS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacgccctg gtcgactacg ccggccccgc cgccaccggc ggtccggtgg cgcggctgac gctgaactca ccgcacaacc gcaacgcgct gtccaccgcg ctggtcagcc agttgcatca ggggctgcgc gacgcctcgt cggatccggc ggtgcgcgtg gtggtgctgg cccataccgg cggcaccttc tgcgcgggtg cggatctcag cgaggccggg tccggcgggt cgccctcgtc ggcctacgac atggccgtcg agcgggcccg ggagatggcc gccctgatgc gcgccatcgt cgaatcccga ctgccggtga tcgccgcgat cgacggccac gtccgggccg gcgggttcgg cttggtgggc gcctgcgaca tcgcggtcgc cggcccgcgc agcagcttcg cgctgaccga ggcgcggatc ggcgtcgccc cggccatcat ctcgctgacg ctgctgccga agctgtcggc gcgcgcggcg gcgcgctact acctgaccgg cgagaagttc gacgcccggc gggccgagga gatcgggttg atcaccatgg ccgccgagga cctagacgcc gcgatcgacc aactcgtcac cgacgtgggc cgcggttcgc cgcagggcct cgcggcgtcc aaggcactga ccaccgccgc ggtgctggag cgcttcgacc gggacgccga gcggctggct gaggaatccg cccggctgtt cgtgtccgac gaagcccgcg aaggcatgct ggcgttcctg gagaagcggt cacccaactg gacgagtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MyavA.01530.a.A1.PW29030
| PURIFICATION DATe: | 6/28/2010 |
| CONCENTRATION: | 21.81mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDALVDYAG PAATGGPVAR LTLNSPHNRN ALSTALVSQL HQGLRDASSD PAVRVVVLAH TGGTFCAGAD LSEAGSGGSP SSAYDMAVER AREMAALMRA IVESRLPVIA AIDGHVRAGG FGLVGACDIA VAGPRSSFAL TEARIGVAPA IISLTLLPKL SARAAARYYL TGEKFDARRA EEIGLITMAA EDLDAAIDQL VTDVGRGSPQ GLAASKALTT AAVLERFDRD AERLAEESAR LFVSDEAREG MLAFLEKRSP NWTS
Validated NT Sequence
ttattaactc gtccagttgg gtgaccgctt ctccaggaac gccagcatgc cttcgcgggc ttcgtcggac acgaacagcc gggcggattc ctcagccagc cgctcggcgt cccggtcgaa gcgctccagc accgcggcgg tggtcagtgc cttggacgcc gcgaggccct gcggcgaacc gcggcccacg tcggtgacga gttggtcgat cgcggcgtct aggtcctcgg cggccatggt gatcaacccg atctcctcgg cccgccgggc gtcgaacttc tcgccggtca ggtagtagcg cgccgccgcg cgcgccgaca gcttcggcag cagcgtcagc gagatgatgg ccggggcgac gccgatccgc gcctcggtca gcgcgaagct gctgcgcggg ccggcgaccg cgatgtcgca ggcgcccacc aagccgaacc cgccggcccg gacgtggccg tcgatcgcgg cgatcaccgg cagtcgggat tcgacgatgg cgcgcatcag ggcggccatc tcccgggccc gctcgacggc catgtcgtag gccgacgagg gcgacccgcc ggacccggcc tcgctgagat ccgcacccgc gcagaaggtg ccgccggtat gggccagcac caccacgcgc accgccggat ccgacgaggc gtcgcgcagc ccctgatgca actggctgac cagcgcggtg gacagcgcgt tgcggttgtg cggtgagttc agcgtcagcc gcgccaccgg accgccggtg gcggcggggc cggcgtagtc gaccagggcg tccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDALVDYAG PAATGGPVAR LTLNSPHNRN ALSTALVSQL HQGLRDASSD PAVRVVVLAH TGGTFCAGAD LSEAGSGGSP SSAYDMAVER AREMAALMRA IVESRLPVIA AIDGHVRAGG FGLVGACDIA VAGPRSSFAL TEARIGVAPA IISLTLLPKL SARAAARYYL TGEKFDARRA EEIGLITMAA EDLDAAIDQL VTDVGRGSPQ GLAASKALTT AAVLERFDRD AERLAEESAR LFVSDEAREG MLAFLEKRSP NWTS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACGCCCTG GTCGACTACG CCGGCCCCGC CGCCACCGGC GGTCCGGTGG CGCGGCTGAC GCTGAACTCA CCGCACAACC GCAACGCGCT GTCCACCGCG CTGGTCAGCC AGTTGCATCA GGGGCTGCGC GACGCCTCGT CGGATCCGGC GGTGCGCGTG GTGGTGCTGG CCCATACCGG CGGCACCTTC TGCGCGGGTG CGGATCTCAG CGAGGCCGGG TCCGGCGGGT CGCCCTCGTC GGCCTACGAC ATGGCCGTCG AGCGGGCCCG GGAGATGGCC GCCCTGATGC GCGCCATCGT CGAATCCCGA CTGCCGGTGA TCGCCGCGAT CGACGGCCAC GTCCGGGCCG GCGGGTTCGG CTTGGTGGGC GCCTGCGACA TCGCGGTCGC CGGCCCGCGC AGCAGCTTCG CGCTGACCGA GGCGCGGATC GGCGTCGCCC CGGCCATCAT CTCGCTGACG CTGCTGCCGA AGCTGTCGGC GCGCGCGGCG GCGCGCTACT ACCTGACCGG CGAGAAGTTC GACGCCCGGC GGGCCGAGGA GATCGGGTTG ATCACCATGG CCGCCGAGGA CCTAGACGCC GCGATCGACC AACTCGTCAC CGACGTGGGC CGCGGTTCGC CGCAGGGCCT CGCGGCGTCC AAGGCACTGA CCACCGCCGC GGTGCTGGAG CGCTTCGACC GGGACGCCGA GCGGCTGGCT GAGGAATCCG CCCGGCTGTT CGTGTCCGAC GAAGCCCGCG AAGGCATGCT GGCGTTCCTG GAGAAGCGGT CACCCAACTG GACGAGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA
Details for MyavA.01530.a.A1.PW29874
| PURIFICATION DATe: | 6/28/2010 |
| CONCENTRATION: | 25.89mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDALVDYAG PAATGGPVAR LTLNSPHNRN ALSTALVSQL HQGLRDASSD PAVRVVVLAH TGGTFCAGAD LSEAGSGGSP SSAYDMAVER AREMAALMRA IVESRLPVIA AIDGHVRAGG FGLVGACDIA VAGPRSSFAL TEARIGVAPA IISLTLLPKL SARAAARYYL TGEKFDARRA EEIGLITMAA EDLDAAIDQL VTDVGRGSPQ GLAASKALTT AAVLERFDRD AERLAEESAR LFVSDEAREG MLAFLEKRSP NWTS
Validated NT Sequence
ttattaactc gtccagttgg gtgaccgctt ctccaggaac gccagcatgc cttcgcgggc ttcgtcggac acgaacagcc gggcggattc ctcagccagc cgctcggcgt cccggtcgaa gcgctccagc accgcggcgg tggtcagtgc cttggacgcc gcgaggccct gcggcgaacc gcggcccacg tcggtgacga gttggtcgat cgcggcgtct aggtcctcgg cggccatggt gatcaacccg atctcctcgg cccgccgggc gtcgaacttc tcgccggtca ggtagtagcg cgccgccgcg cgcgccgaca gcttcggcag cagcgtcagc gagatgatgg ccggggcgac gccgatccgc gcctcggtca gcgcgaagct gctgcgcggg ccggcgaccg cgatgtcgca ggcgcccacc aagccgaacc cgccggcccg gacgtggccg tcgatcgcgg cgatcaccgg cagtcgggat tcgacgatgg cgcgcatcag ggcggccatc tcccgggccc gctcgacggc catgtcgtag gccgacgagg gcgacccgcc ggacccggcc tcgctgagat ccgcacccgc gcagaaggtg ccgccggtat gggccagcac caccacgcgc accgccggat ccgacgaggc gtcgcgcagc ccctgatgca actggctgac cagcgcggtg gacagcgcgt tgcggttgtg cggtgagttc agcgtcagcc gcgccaccgg accgccggtg gcggcggggc cggcgtagtc gaccagggcg tccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDALVDYAG PAATGGPVAR LTLNSPHNRN ALSTALVSQL HQGLRDASSD PAVRVVVLAH TGGTFCAGAD LSEAGSGGSP SSAYDMAVER AREMAALMRA IVESRLPVIA AIDGHVRAGG FGLVGACDIA VAGPRSSFAL TEARIGVAPA IISLTLLPKL SARAAARYYL TGEKFDARRA EEIGLITMAA EDLDAAIDQL VTDVGRGSPQ GLAASKALTT AAVLERFDRD AERLAEESAR LFVSDEAREG MLAFLEKRSP NWTS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACGCCCTG GTCGACTACG CCGGCCCCGC CGCCACCGGC GGTCCGGTGG CGCGGCTGAC GCTGAACTCA CCGCACAACC GCAACGCGCT GTCCACCGCG CTGGTCAGCC AGTTGCATCA GGGGCTGCGC GACGCCTCGT CGGATCCGGC GGTGCGCGTG GTGGTGCTGG CCCATACCGG CGGCACCTTC TGCGCGGGTG CGGATCTCAG CGAGGCCGGG TCCGGCGGGT CGCCCTCGTC GGCCTACGAC ATGGCCGTCG AGCGGGCCCG GGAGATGGCC GCCCTGATGC GCGCCATCGT CGAATCCCGA CTGCCGGTGA TCGCCGCGAT CGACGGCCAC GTCCGGGCCG GCGGGTTCGG CTTGGTGGGC GCCTGCGACA TCGCGGTCGC CGGCCCGCGC AGCAGCTTCG CGCTGACCGA GGCGCGGATC GGCGTCGCCC CGGCCATCAT CTCGCTGACG CTGCTGCCGA AGCTGTCGGC GCGCGCGGCG GCGCGCTACT ACCTGACCGG CGAGAAGTTC GACGCCCGGC GGGCCGAGGA GATCGGGTTG ATCACCATGG CCGCCGAGGA CCTAGACGCC GCGATCGACC AACTCGTCAC CGACGTGGGC CGCGGTTCGC CGCAGGGCCT CGCGGCGTCC AAGGCACTGA CCACCGCCGC GGTGCTGGAG CGCTTCGACC GGGACGCCGA GCGGCTGGCT GAGGAATCCG CCCGGCTGTT CGTGTCCGAC GAAGCCCGCG AAGGCATGCT GGCGTTCCTG GAGAAGCGGT CACCCAACTG GACGAGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA