MyavA.01455.a

Carbamoyl-phosphate synthase, small subunit

CENTER ID: MyavA.01455.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01455.a.A1.GE29123 Full length( MyavA.01455.a ) 1 373
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.3319
RefSeq: YP_882572.1
UniProt: A0A0H2ZZG6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGKAQLVLE DGRVFTGTPF GAIGQTLGEA VFSTGMSGYQ ETLTDPSYHR QIVVATAPQI GNTGWNGEDA ESRGDKIWVA GYAVRDPSPR VSNWRATGSL EDELIRQRIV GIARIDTRAV VRHLRTRGSM KAGVFSGDAL ADPDELVQRV RGQQSMLGAD LAGEVSTPDA YIVEPEGPPR FTVAALDLGI KTNTPRNFAR RGIRSHVLPS SATFEQIADL RPDGVFLSNG PGDPATADHI VAVTREVLGA GIPLFGICFG NQILGRALGL STYKMVFGHR GINIPVIDHA TGRVAVTAQN HGFALQGEAG QAFDTPFGAA VVSHTCANDG VVEGVKLADG RAFSVQYHPE AAAGPHDANY LFDQFIELME GDR
NT Sequence
GTGAGTGGCA AGGCGCAACT GGTGCTCGAG GACGGCCGCG TCTTCACCGG CACACCGTTC GGGGCGATCG GCCAAACCCT GGGCGAGGCC GTGTTTTCCA CCGGCATGTC CGGCTACCAG GAGACGTTGA CCGACCCCAG CTACCACCGC CAGATCGTGG TGGCCACCGC GCCGCAGATC GGCAATACCG GCTGGAACGG CGAGGACGCC GAGAGCCGCG GCGACAAGAT CTGGGTGGCC GGCTACGCGG TGCGCGACCC GTCGCCGCGC GTGTCCAACT GGCGCGCCAC CGGAAGCCTG GAGGACGAGC TGATCCGCCA GCGCATCGTG GGCATCGCTC GCATCGACAC CCGCGCGGTG GTGCGCCACC TGCGGACCCG CGGTTCGATG AAGGCGGGGG TGTTCTCCGG TGACGCCCTG GCCGATCCGG ACGAACTGGT GCAGCGGGTG CGCGGCCAGC AGTCCATGCT GGGCGCCGAC CTGGCCGGCG AGGTCAGCAC GCCGGACGCC TACATCGTGG AACCCGAAGG GCCGCCGCGG TTTACCGTCG CCGCCCTGGA CCTGGGCATC AAAACCAACA CGCCGCGCAA CTTCGCCCGT CGCGGCATCC GCAGCCACGT GCTGCCCTCG TCGGCGACCT TCGAGCAGAT CGCCGACCTC AGGCCGGACG GCGTGTTCCT GTCCAACGGG CCCGGCGACC CCGCCACCGC CGACCACATC GTGGCGGTCA CCCGCGAGGT GCTGGGCGCC GGAATCCCAT TGTTCGGCAT CTGTTTCGGC AACCAGATCC TGGGCCGGGC GCTGGGCCTG TCCACCTACA AGATGGTGTT CGGTCACCGC GGCATCAACA TCCCGGTGAT CGACCACGCC ACCGGGCGGG TGGCGGTGAC CGCGCAGAAC CACGGCTTCG CGCTGCAGGG CGAGGCCGGC CAGGCCTTCG ACACGCCGTT CGGCGCGGCG GTGGTCAGCC ACACCTGCGC CAACGACGGG GTGGTGGAGG GCGTGAAACT CGCTGACGGA CGGGCATTTT CGGTGCAGTA CCACCCGGAG GCGGCGGCCG GCCCGCACGA CGCGAACTAC CTCTTCGACC AATTCATCGA GCTGATGGAA GGGGACCGC
Details for MyavA.01455.a.A1.GE29123
HARVESTED ON: 4/27/2010
SEQUENCED ON: 5/1/2010
EXPECTED MW: 42kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MVSGKAQLVL EDGRVFTGTP FGAIGQTLGE AVFSTGMSGY QETLTDPSYH RQIVVATAPQ IGNTGWNGED AESRGDKIWV AGYAVRDPSP RVSNWRATGS LEDELIRQRI VGIARIDTRA VVRHLRTRGS MKAGVFSGDA LADPDELVQR VRGQQSMLGA DLAGEVSTPD AYIVEPEGPP RFTVAALDLG IKTNTPRNFA RRGIRSHVLP SSATFEQIAD LRPDGVFLSN GPGDPATADH IVAVTREVLG AGIPLFGICF GNQILGRALG LSTYKMVFGH RGINIPVIDH ATGRVAVTAQ NHGFALQGEA GQAFDTPFGA AVVSHTCAND GVVEGVKLAD GRAFSVQYHP EAAAGPHDAN YLFDQFIELM EG
Validated NT Sequence
ccccttccat cagctcgatg aattggtcga agaggtagtt cgcgtcgtgc gggccggccg ccgcctccgg gtggtactgc accgaaaatg cccgtccgtc agcgagtttc acgccctcca ccaccccgtc gttggcgcag gtgtggctga ccaccgccgc gccgaacggc gtgtcgaagg cctggccggc ctcgccctgc agcgcgaagc cgtggttctg cgcggtcacc gccacccgcc cggtggcgtg gtcgatcacc gggatgttga tgccgcggtg accgaacacc atcttgtagg tggacaggcc cagcgcccgg cccaggatct ggttgccgaa acagatgccg aacaatggga ttccggcgcc cagcacctcg cgggtgaccg ccacgatgtg gtcggcggtg gcggggtcgc cgggcccgtt ggacaggaac acgccgtccg gcctgaggtc ggcgatctgc tcgaaggtcg ccgacgaggg cagcacgtgg ctgcggatgc cgcgacgggc gaagttgcgc ggcgtgttgg ttttgatgcc caggtccagg gcggcgacgg taaaccgcgg cggcccttcg ggttccacga tgtaggcgtc cggcgtgctg acctcgccgg ccaggtcggc gcccagcatg gactgctggc cgcgcacccg ctgcaccagt tcgtccggat cggccagggc gtcaccggag aacacccccg ccttcatcga accgcgggtc cgcaggtggc gcaccaccgc gcgggtgtcg atgcgagcga tgcccacgat gcgctggcgg atcagctcgt cctccaggct tccggtggcg cgccagttgg acacgcgcgg cgacgggtcg cgcaccgcgt agccggccac ccagatcttg tcgccgcggc tctcggcgtc ctcgccgttc cagccggtat tgccgatctg cggcgcggtg gccaccacga tctggcggtg gtagctgggg tcggtcaacg tctcctggta gccggacatg ccggtggaaa acacggcctc gcccagggtt tggccgatcg ccccgaacgg tgtgccggtg aagacgcggc cgtcctcgag caccagttgc gccttgccac tcaccatcga accaggaccc tgggtctgag cttccagggt acc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSGKAQLV LEDGRVFTGT PFGAIGQTLG EAVFSTGMSG YQETLTDPSY HRQIVVATAP QIGNTGWNGE DAESRGDKIW VAGYAVRDPS PRVSNWRATG SLEDELIRQR IVGIARIDTR AVVRHLRTRG SMKAGVFSGD ALADPDELVQ RVRGQQSMLG ADLAGEVSTP DAYIVEPEGP PRFTVAALDL GIKTNTPRNF ARRGIRSHVL PSSATFEQIA DLRPDGVFLS NGPGDPATAD HIVAVTREVL GAGIPLFGIC FGNQILGRAL GLSTYKMVFG HRGINIPVID HATGRVAVTA QNHGFALQGE AGQAFDTPFG AAVVSHTCAN DGVVEGVKLA DGRAFSVQYH PEAAAGPHDA NYLFDQFIEL MEGDR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtggc aaggcgcaac tggtgctcga ggacggccgc gtcttcaccg gcacaccgtt cggggcgatc ggccaaaccc tgggcgaggc cgtgttttcc accggcatgt ccggctacca ggagacgttg accgacccca gctaccaccg ccagatcgtg gtggccaccg cgccgcagat cggcaatacc ggctggaacg gcgaggacgc cgagagccgc ggcgacaaga tctgggtggc cggctacgcg gtgcgcgacc cgtcgccgcg cgtgtccaac tggcgcgcca ccggaagcct ggaggacgag ctgatccgcc agcgcatcgt gggcatcgct cgcatcgaca cccgcgcggt ggtgcgccac ctgcggaccc gcggttcgat gaaggcgggg gtgttctccg gtgacgccct ggccgatccg gacgaactgg tgcagcgggt gcgcggccag cagtccatgc tgggcgccga cctggccggc gaggtcagca cgccggacgc ctacatcgtg gaacccgaag ggccgccgcg gtttaccgtc gccgccctgg acctgggcat caaaaccaac acgccgcgca acttcgcccg tcgcggcatc cgcagccacg tgctgccctc gtcggcgacc ttcgagcaga tcgccgacct caggccggac ggcgtgttcc tgtccaacgg gcccggcgac cccgccaccg ccgaccacat cgtggcggtc acccgcgagg tgctgggcgc cggaatccca ttgttcggca tctgtttcgg caaccagatc ctgggccggg cgctgggcct gtccacctac aagatggtgt tcggtcaccg cggcatcaac atcccggtga tcgaccacgc caccgggcgg gtggcggtga ccgcgcagaa ccacggcttc gcgctgcagg gcgaggccgg ccaggccttc gacacgccgt tcggcgcggc ggtggtcagc cacacctgcg ccaacgacgg ggtggtggag ggcgtgaaac tcgctgacgg acgggcattt tcggtgcagt accacccgga ggcggcggcc ggcccgcacg acgcgaacta cctcttcgac caattcatcg agctgatgga aggggaccgc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa