SSGCID: Target Page

MyavA.01395.a

Thiosulfate sulfurtransferase

CENTER ID:	MyavA.01395.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01395.a.A1.GE29120	Full length( MyavA.01395.a )		1	284

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01395.a.A1.PS00727	Full length( MyavA.01395.a )		1	284

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.2091
RefSeq:	YP_881352.1
UniProt:	A0A0H2ZX30

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGARDQVLIT ATELADVIEA GDPVSILDVR WRLDEPDGRA AYLQGHLPGA VYVSLEDELS DHTVSGRGRH PLPSGPSLQA AARRWGIRQD TPVVVYDDWN RAGSARAWWL LRAAGLDNVR ILDGGLAAWR AAGGRLVSGP VEPAPGNVTV PHDDLHSGNR PTVTTEQVTA GAATLIDARA PERYRGEMEP LDPVAGHIPG AENLPSGDVL AADGTFLDDD ALARVFAEHR IERHGAVAAY CGSGVTATVT IAALAAVGRT AALYPGSWSE WCADPARPVE RGGA

NT Sequence

GTGGGGGCAC GCGATCAGGT ATTGATCACC GCGACCGAGT TGGCCGACGT CATCGAGGCC GGAGACCCGG TGAGCATCCT GGACGTGCGG TGGAGGCTCG ACGAGCCCGA CGGCCGCGCG GCCTACCTGC AGGGCCACCT TCCCGGCGCG GTGTACGTCT CGCTCGAGGA CGAGCTCAGC GACCACACGG TGTCCGGGCG GGGCCGTCAC CCGCTGCCGT CGGGACCCAG CCTGCAGGCG GCCGCCCGCC GATGGGGCAT CCGGCAGGAC ACACCGGTGG TGGTCTACGA CGACTGGAAC CGCGCCGGCT CCGCGCGCGC CTGGTGGCTG CTGCGCGCGG CCGGGCTGGA CAACGTCCGC ATTCTGGACG GCGGGCTGGC CGCCTGGCGG GCGGCCGGCG GCCGACTCGT GTCCGGGCCC GTCGAACCGG CGCCCGGGAA TGTGACTGTG CCGCATGATG ATTTGCATTC CGGCAACCGT CCGACCGTGA CGACCGAGCA GGTGACCGCG GGCGCCGCCA CACTCATCGA CGCGCGGGCG CCGGAACGCT ACCGCGGCGA AATGGAACCA CTCGATCCCG TCGCCGGGCA CATTCCCGGC GCCGAGAATC TGCCCAGCGG CGACGTTTTG GCGGCCGACG GCACCTTCCT CGACGACGAC GCGCTCGCCC GGGTGTTCGC CGAGCACCGA ATCGAGCGGC ACGGCGCGGT GGCCGCCTAT TGCGGCTCGG GCGTCACCGC CACGGTGACG ATCGCCGCGC TCGCGGCGGT GGGCCGAACG GCGGCGCTGT ACCCGGGATC GTGGTCGGAG TGGTGCGCGG ACCCGGCCCG CCCAGTGGAA CGCGGCGGCG CG

Details for MyavA.01395.a.A1.GE29120

HARVESTED ON:	4/27/2010
SEQUENCED ON:	5/1/2010
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVGARDQVL ITATELADVI EAGDPVSILD VRWRLDEPDG RAAYLQGHLP GAVYVSLEDE LSDHTVSGRG RHPLPSGPSL QAAARRWGIR QDTPVVVYDD WNRAGSARAW WLLRAAGLDN VRILDGGLAA WRAAGGRLVS GPVEPAPGNV TVPHDDLHSG NRPTVTTEQV TAGAATLIDA RAPERYRGEM EPLDPVAGHI PGAENLPSGD VLAADGTFLD DDALARVFAE HRIERHGAVA AYCGSGVTAT VTIAALAAVG RTAALYPGSW SEWCADPARP VERGGA

Validated NT Sequence

ttattacgcg ccgccgcgtt ccactgggcg ggccgggtcc gcgcaccact ccgaccacga tcccgggtac agcgccgccg ttcggcccac cgccgcgagc gcggcgatcg tcaccgtggc ggtgacgccc gagccgcaat aggcggccac cgcgccgtgc cgctcgattc ggtgctcggc gaacacccgg gcgagcgcgt cgtcgtcgag gaaggtgccg tcggccgcca aaacgtcgcc gctgggcaga ttctcggcgc cgggaatgtg cccggcgacg ggatcgagtg gttccatttc gccgcggtag cgttccggcg cccgcgcgtc gatgagtgtg gcggcgcccg cggtcacctg ctcggtcgtc acggtcggac ggttgccgga atgcaaatca tcatgcggca cagtcacatt cccgggcgcc ggttcgacgg gcccggacac gagtcggccg ccggccgccc gccaggcggc cagcccgccg tccagaatgc ggacgttgtc cagcccggcc gcgcgcagca gccaccaggc gcgcgcggag ccggcgcggt tccagtcgtc gtagaccacc accggtgtgt cctgccggat gccccatcgg cgggcggccg cctgcaggct gggtcccgac ggcagcgggt gacggccccg cccggacacc gtgtggtcgc tgagctcgtc ctcgagcgag acgtacaccg cgccgggaag gtggccctgc aggtaggccg cgcggccgtc gggctcgtcg agcctccacc gcacgtccag gatgctcacc gggtctccgg cctcgatgac gtcggccaac tcggtcgcgg tgatcaatac ctgatcgcgt gcccccacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgggggca cgcgatcagg tattgatcac cgcgaccgag ttggccgacg tcatcgaggc cggagacccg gtgagcatcc tggacgtgcg gtggaggctc gacgagcccg acggccgcgc ggcctacctg cagggccacc ttcccggcgc ggtgtacgtc tcgctcgagg acgagctcag cgaccacacg gtgtccgggc ggggccgtca cccgctgccg tcgggaccca gcctgcaggc ggccgcccgc cgatggggca tccggcagga cacaccggtg gtggtctacg acgactggaa ccgcgccggc tccgcgcgcg cctggtggct gctgcgcgcg gccgggctgg acaacgtccg cattctggac ggcgggctgg ccgcctggcg ggcggccggc ggccgactcg tgtccgggcc cgtcgaaccg gcgcccggga atgtgactgt gccgcatgat gatttgcatt ccggcaaccg tccgaccgtg acgaccgagc aggtgaccgc gggcgccgcc acactcatcg acgcgcgggc gccggaacgc taccgcggcg aaatggaacc actcgatccc gtcgccgggc acattcccgg cgccgagaat ctgcccagcg gcgacgtttt ggcggccgac ggcaccttcc tcgacgacga cgcgctcgcc cgggtgttcg ccgagcaccg aatcgagcgg cacggcgcgg tggccgccta ttgcggctcg ggcgtcaccg ccacggtgac gatcgccgcg ctcgcggcgg tgggccgaac ggcggcgctg tacccgggat cgtggtcgga gtggtgcgcg gacccggccc gcccagtgga acgcggcggc gcgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for MyavA.01395.a.A1.PS00727

PURIFICATION DATe:	8/19/2010
CONCENTRATION:	35.5mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGGGGCA CGCGATCAGG TATTGATCAC CGCGACCGAG TTGGCCGACG TCATCGAGGC CGGAGACCCG GTGAGCATCC TGGACGTGCG GTGGAGGCTC GACGAGCCCG ACGGCCGCGC GGCCTACCTG CAGGGCCACC TTCCCGGCGC GGTGTACGTC TCGCTCGAGG ACGAGCTCAG CGACCACACG GTGTCCGGGC GGGGCCGTCA CCCGCTGCCG TCGGGACCCA GCCTGCAGGC GGCCGCCCGC CGATGGGGCA TCCGGCAGGA CACACCGGTG GTGGTCTACG ACGACTGGAA CCGCGCCGGC TCCGCGCGCG CCTGGTGGCT GCTGCGCGCG GCCGGGCTGG ACAACGTCCG CATTCTGGAC GGCGGGCTGG CCGCCTGGCG GGCGGCCGGC GGCCGACTCG TGTCCGGGCC CGTCGAACCG GCGCCCGGGA ATGTGACTGT GCCGCATGAT GATTTGCATT CCGGCAACCG TCCGACCGTG ACGACCGAGC AGGTGACCGC GGGCGCCGCC ACACTCATCG ACGCGCGGGC GCCGGAACGC TACCGCGGCG AAATGGAACC ACTCGATCCC GTCGCCGGGC ACATTCCCGG CGCCGAGAAT CTGCCCAGCG GCGACGTTTT GGCGGCCGAC GGCACCTTCC TCGACGACGA CGCGCTCGCC CGGGTGTTCG CCGAGCACCG AATCGAGCGG CACGGCGCGG TGGCCGCCTA TTGCGGCTCG GGCGTCACCG CCACGGTGAC GATCGCCGCG CTCGCGGCGG TGGGCCGAAC GGCGGCGCTG TACCCGGGAT CGTGGTCGGA GTGGTGCGCG GACCCGGCCC GCCCAGTGGA ACGCGGCGGC GCGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA