MyavA.01381.a
Enoyl-CoA hydratase/isomerase
| CENTER ID: | MyavA.01381.a |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01381.a.A1.GE29119 | Full length( MyavA.01381.a ) | 1 | 251 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01381.a.A1.PS00696 | Full length( MyavA.01381.a ) | 1 | 251 |
Structures
3P5M
DEPOSITED: 10/8/2010
DETERMINATION: XRay
CLONE: MyavA.01381.a.A1.GE29119
PROTEIN: MyavA.01381.a.A1.PS00696
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_153107 |
| PATRIC ID: | fig|243243.7.peg.2538 |
| RefSeq: | YP_881792.1 |
| UniProt: | A0A0H2ZZ96 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNGISVEHDG AVLRIRLDRP EKLNAVDTPM LEELSVHIRD AEADESVRAV LLTGAGRAFC SGGDLTGGDT AGAADAANRV VRAITSLPKP VIAGVHGAAV GFGCSLALAC DLVVAAPASY FQLAFTRVGL MPDGGASALL PLLIGRARTS RMAMTAEKIS AATAFEWGMI SHITSADEYE SVLTDVLRSV SGGPTLAFGW TKRALAAATL AELEPVQAIE AEGQLALVET ADFREGARAF RERRTPNFRG H
NT Sequence
ATGAACGGCA TCTCGGTCGA GCACGACGGC GCGGTGCTGC GGATCCGGCT GGACCGACCG GAGAAGCTCA ACGCCGTCGA CACCCCCATG CTCGAGGAGT TGTCGGTTCA CATTCGCGAT GCCGAGGCAG ACGAATCGGT CCGCGCGGTG CTGCTCACCG GTGCCGGTCG AGCATTCTGT TCCGGCGGCG ACCTGACCGG CGGTGACACT GCGGGCGCCG CTGATGCCGC CAACCGGGTG GTCCGGGCGA TCACCTCGCT ACCCAAGCCG GTGATCGCCG GTGTCCATGG AGCGGCCGTT GGCTTCGGGT GTTCGCTGGC GCTCGCCTGC GATCTGGTGG TGGCCGCGCC AGCGTCGTAC TTTCAGTTGG CCTTTACCCG GGTCGGGCTG ATGCCGGACG GGGGTGCGTC CGCGCTGCTG CCATTGCTGA TTGGTCGAGC GCGCACCTCT CGCATGGCCA TGACCGCGGA AAAGATCTCC GCTGCAACCG CATTTGAATG GGGGATGATC TCGCACATCA CTTCCGCGGA CGAATACGAG TCGGTGCTGA CCGACGTGCT GCGGTCGGTC TCCGGCGGTC CAACGTTGGC GTTCGGCTGG ACGAAGCGTG CGCTGGCCGC AGCGACCCTG GCCGAGCTCG AGCCGGTGCA AGCTATCGAA GCCGAGGGGC AGTTGGCGTT GGTCGAGACC GCTGACTTCA GAGAAGGGGC ACGCGCCTTC CGCGAGCGGC GCACCCCAAA TTTTCGTGGC CAC
Details for MyavA.01381.a.A1.GE29119
| HARVESTED ON: | 4/27/2010 |
| SEQUENCED ON: | 5/1/2010 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNGISVEHD GAVLRIRLDR PEKLNAVDTP MLEELSVHIR DAEADESVRA VLLTGAGRAF CSGGDLTGGD TAGAADAANR VVRAITSLPK PVIAGVHGAA VGFGCSLALA CDLVVAAPAS YFQLAFTRVG LMPDGGASAL LPLLIGRART SRMAMTAEKI SAATAFEWGM ISHITSADEY ESVLTDVLRS VSGGPTLAFG WTKRALAAAT LAELEPVQAI EAEGQLALVE TADFREGARA FRERRTPNFR GH
Validated NT Sequence
tattagtggc cacgaaaatt tggggtgcgc cgctcgcgga aggcgcgtgc cccttctctg aagtcagcgg tctcgaccaa cgccaactgc ccctcggctt cgatagcttg caccggctcg agctcggcca gggtcgctgc ggccagcgca cgcttcgtcc agccgaacgc caacgttgga ccgccggaga ccgaccgcag cacgtcggtc agcaccgact cgtattcgtc cgcggaagtg atgtgcgaga tcatccccca ttcaaatgcg gttgcagcgg agatcttttc cgcggtcatg gccatgcgag aggtgcgcgc tcgaccaatc agcaatggca gcagcgcgga cgcacccccg tccggcatca gcccgacccg ggtaaaggcc aactgaaagt acgacgctgg cgcggccacc accagatcgc aggcgagcgc cagcgaacac ccgaagccaa cggccgctcc atggacaccg gcgatcaccg gcttgggtag cgaggtgatc gcccggacca cccggttggc ggcatcagcg gcgcccgcag tgtcaccgcc ggtcaggtcg ccgccggaac agaatgctcg accggcaccg gtgagcagca ccgcgcggac cgattcgtct gcctcggcat cgcgaatgtg aaccgacaac tcctcgagca tgggggtgtc gacggcgttg agcttctccg gtcggtccag ccggatccgc agcaccgcgc cgtcgtgctc gaccgagatg ccgttcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNGISVEHD GAVLRIRLDR PEKLNAVDTP MLEELSVHIR DAEADESVRA VLLTGAGRAF CSGGDLTGGD TAGAADAANR VVRAITSLPK PVIAGVHGAA VGFGCSLALA CDLVVAAPAS YFQLAFTRVG LMPDGGASAL LPLLIGRART SRMAMTAEKI SAATAFEWGM ISHITSADEY ESVLTDVLRS VSGGPTLAFG WTKRALAAAT LAELEPVQAI EAEGQLALVE TADFREGARA FRERRTPNFR GH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacggcatc tcggtcgagc acgacggcgc ggtgctgcgg atccggctgg accgaccgga gaagctcaac gccgtcgaca cccccatgct cgaggagttg tcggttcaca ttcgcgatgc cgaggcagac gaatcggtcc gcgcggtgct gctcaccggt gccggtcgag cattctgttc cggcggcgac ctgaccggcg gtgacactgc gggcgccgct gatgccgcca accgggtggt ccgggcgatc acctcgctac ccaagccggt gatcgccggt gtccatggag cggccgttgg cttcgggtgt tcgctggcgc tcgcctgcga tctggtggtg gccgcgccag cgtcgtactt tcagttggcc tttacccggg tcgggctgat gccggacggg ggtgcgtccg cgctgctgcc attgctgatt ggtcgagcgc gcacctctcg catggccatg accgcggaaa agatctccgc tgcaaccgca tttgaatggg ggatgatctc gcacatcact tccgcggacg aatacgagtc ggtgctgacc gacgtgctgc ggtcggtctc cggcggtcca acgttggcgt tcggctggac gaagcgtgcg ctggccgcag cgaccctggc cgagctcgag ccggtgcaag ctatcgaagc cgaggggcag ttggcgttgg tcgagaccgc tgacttcaga gaaggggcac gcgccttccg cgagcggcgc accccaaatt ttcgtggcca caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MyavA.01381.a.A1.PS00696
| PURIFICATION DATe: | 7/29/2010 |
| CONCENTRATION: | 19.25mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNGISVEHD GAVLRIRLDR PEKLNAVDTP MLEELSVHIR DAEADESVRA VLLTGAGRAF CSGGDLTGGD TAGAADAANR VVRAITSLPK PVIAGVHGAA VGFGCSLALA CDLVVAAPAS YFQLAFTRVG LMPDGGASAL LPLLIGRART SRMAMTAEKI SAATAFEWGM ISHITSADEY ESVLTDVLRS VSGGPTLAFG WTKRALAAAT LAELEPVQAI EAEGQLALVE TADFREGARA FRERRTPNFR GH
Validated NT Sequence
tattagtggc cacgaaaatt tggggtgcgc cgctcgcgga aggcgcgtgc cccttctctg aagtcagcgg tctcgaccaa cgccaactgc ccctcggctt cgatagcttg caccggctcg agctcggcca gggtcgctgc ggccagcgca cgcttcgtcc agccgaacgc caacgttgga ccgccggaga ccgaccgcag cacgtcggtc agcaccgact cgtattcgtc cgcggaagtg atgtgcgaga tcatccccca ttcaaatgcg gttgcagcgg agatcttttc cgcggtcatg gccatgcgag aggtgcgcgc tcgaccaatc agcaatggca gcagcgcgga cgcacccccg tccggcatca gcccgacccg ggtaaaggcc aactgaaagt acgacgctgg cgcggccacc accagatcgc aggcgagcgc cagcgaacac ccgaagccaa cggccgctcc atggacaccg gcgatcaccg gcttgggtag cgaggtgatc gcccggacca cccggttggc ggcatcagcg gcgcccgcag tgtcaccgcc ggtcaggtcg ccgccggaac agaatgctcg accggcaccg gtgagcagca ccgcgcggac cgattcgtct gcctcggcat cgcgaatgtg aaccgacaac tcctcgagca tgggggtgtc gacggcgttg agcttctccg gtcggtccag ccggatccgc agcaccgcgc cgtcgtgctc gaccgagatg ccgttcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNGISVEHD GAVLRIRLDR PEKLNAVDTP MLEELSVHIR DAEADESVRA VLLTGAGRAF CSGGDLTGGD TAGAADAANR VVRAITSLPK PVIAGVHGAA VGFGCSLALA CDLVVAAPAS YFQLAFTRVG LMPDGGASAL LPLLIGRART SRMAMTAEKI SAATAFEWGM ISHITSADEY ESVLTDVLRS VSGGPTLAFG WTKRALAAAT LAELEPVQAI EAEGQLALVE TADFREGARA FRERRTPNFR GH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAACGGCATC TCGGTCGAGC ACGACGGCGC GGTGCTGCGG ATCCGGCTGG ACCGACCGGA GAAGCTCAAC GCCGTCGACA CCCCCATGCT CGAGGAGTTG TCGGTTCACA TTCGCGATGC CGAGGCAGAC GAATCGGTCC GCGCGGTGCT GCTCACCGGT GCCGGTCGAG CATTCTGTTC CGGCGGCGAC CTGACCGGCG GTGACACTGC GGGCGCCGCT GATGCCGCCA ACCGGGTGGT CCGGGCGATC ACCTCGCTAC CCAAGCCGGT GATCGCCGGT GTCCATGGAG CGGCCGTTGG CTTCGGGTGT TCGCTGGCGC TCGCCTGCGA TCTGGTGGTG GCCGCGCCAG CGTCGTACTT TCAGTTGGCC TTTACCCGGG TCGGGCTGAT GCCGGACGGG GGTGCGTCCG CGCTGCTGCC ATTGCTGATT GGTCGAGCGC GCACCTCTCG CATGGCCATG ACCGCGGAAA AGATCTCCGC TGCAACCGCA TTTGAATGGG GGATGATCTC GCACATCACT TCCGCGGACG AATACGAGTC GGTGCTGACC GACGTGCTGC GGTCGGTCTC CGGCGGTCCA ACGTTGGCGT TCGGCTGGAC GAAGCGTGCG CTGGCCGCAG CGACCCTGGC CGAGCTCGAG CCGGTGCAAG CTATCGAAGC CGAGGGGCAG TTGGCGTTGG TCGAGACCGC TGACTTCAGA GAAGGGGCAC GCGCCTTCCG CGAGCGGCGC ACCCCAAATT TTCGTGGCCA CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA