MyavA.01379.a
Putative oxidoreductase
| CENTER ID: | MyavA.01379.a |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01379.a.A1.GE29118 | Full length( MyavA.01379.a ) | 1 | 292 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01379.a.A1.PS00711 | Full length( MyavA.01379.a ) | 1 | 292 |
Structures
3QHA
DEPOSITED: 1/25/2011
DETERMINATION: XRay
CLONE: MyavA.01379.a.A1.GE29118
PROTEIN: MyavA.01379.a.A1.PS00711
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126809 |
| PATRIC ID: | fig|243243.7.peg.695 |
| RefSeq: | YP_879990.1 |
| UniProt: | A0A0H2ZSR3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTNAAHTTE QLKLGYIGLG NMGAPMATRM TEWPGGVTVY DIRIEAMTPL AEAGATLADS VADVAAADLI HITVLDDAQV REVVGELAGH AKPGTVIAIH STISDTTAVE LARDLKARDI HIVDAPVSGG AAAAARGELA TMVGADREVY ERIKPAFKHW AAVVIHAGEP GAGTRMKLAR NMLTFTSYAA ACEAMKLAEA AGLDLQALGR VVRHTDALTG GPGAIMVRDN MKDLEPDNFL YQPFLHTRGL GEKDLSLALA LGEAVSVDLP LARLAYEGLA AGLGVPHKEK EA
NT Sequence
ATGACGACGA ACGCGGCGCA CACGACCGAG CAGCTGAAGC TCGGCTACAT CGGGCTGGGC AATATGGGCG CGCCGATGGC CACCAGGATG ACCGAATGGC CGGGCGGGGT AACGGTTTAC GACATCCGGA TCGAGGCGAT GACGCCGCTG GCCGAGGCCG GCGCCACCCT TGCCGACAGC GTGGCCGACG TCGCCGCCGC CGACCTGATC CACATCACCG TGCTCGACGA CGCCCAGGTG CGTGAGGTGG TCGGCGAGCT GGCCGGGCAC GCCAAGCCCG GCACGGTGAT CGCGATCCAC TCGACCATCA GCGACACCAC CGCCGTCGAA CTGGCCCGCG ATCTCAAGGC GCGGGACATC CACATCGTCG ACGCCCCGGT CAGCGGCGGC GCGGCCGCCG CCGCCCGGGG TGAGCTCGCC ACCATGGTGG GCGCCGACCG CGAGGTCTAC GAGCGGATCA AGCCGGCGTT CAAACACTGG GCGGCGGTGG TCATCCACGC CGGTGAACCG GGTGCGGGAA CCCGAATGAA GTTGGCCCGC AACATGTTGA CGTTCACCTC GTATGCCGCA GCGTGCGAGG CGATGAAGTT GGCCGAGGCG GCCGGGCTGG ACCTGCAGGC GCTGGGCCGG GTGGTGCGTC ATACCGACGC GCTCACCGGC GGTCCGGGCG CGATCATGGT GCGCGACAAC ATGAAAGACC TCGAGCCGGA CAACTTCCTC TACCAGCCGT TCTTGCACAC CCGCGGCCTG GGGGAGAAGG ATCTGAGCCT GGCGCTGGCG TTGGGTGAGG CGGTCTCGGT CGACCTGCCG CTGGCCCGGC TGGCGTACGA GGGACTGGCG GCCGGGCTCG GCGTGCCACA CAAGGAGAAA GAGGCG
Details for MyavA.01379.a.A1.GE29118
| HARVESTED ON: | 4/27/2010 |
| SEQUENCED ON: | 5/1/2010 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 92 |
Validated AA Sequence
MTTNAAHTTE QLKLGYIGLG NMGAPMATRM TEWPGGVTVY DIRIEAMTPL AEAGATLADS VADVAAADLI HITVLDDAQV REVVGELAGH AKPGTVIAIH STISDTTAVE LARDLKARDI HIVDAPVSGG AAAAARGELA TMVGADREVY ERIKPAFKHW AAVVIHAGEP GAGTRMKLAR NMLTFTSYAA ACEAMKLAEA AGLDLQALGR VVRHTDALTG GPGAIMVRDN MKDLEPDNFL YQPFLHTRGL GEKDLSLALA LGEAVSVDLP LARLAYEGLA AGLGVPHKEK
Validated NT Sequence
ctttctcctt gtgtggcacg ccgagcccgg ccgccagtcc ctcgtacgcc agccgggcca gcggcaggtc gaccgagacc gcctcaccca acgccagcgc caggctcaga tccttctccc ccaggccgcg ggtgtgcaag aacggctggt agaggaagtt gtccggctcg aggtctttca tgttgtcgcg caccatgatc gcgcccggac cgccggtgag cgcgtcggta tgacgcacca cccggcccag cgcctgcagg tccagcccgg ccgcctcggc caacttcatc gcctcgcacg ctgcggcata cgaggtgaac gtcaacatgt tgcgggccaa cttcattcgg gttcccgcac ccggttcacc ggcgtggatg accaccgccg cccagtgttt gaacgccggc ttgatccgct cgtagacctc gcggtcggcg cccaccatgg tggcgagctc accccgggcg gcggcggccg cgccgccgct gaccggggcg tcgacgatgt ggatgtcccg cgccttgaga tcgcgggcca gttcgacggc ggtggtgtcg ctgatggtcg agtggatcgc gatcaccgtg ccgggcttgg cgtgcccggc cagctcgccg accacctcac gcacctgggc gtcgtcgagc acggtgatgt ggatcaggtc ggcggcggcg acgtcggcca cgctgtcggc aagggtggcg ccggcctcgg ccagcggcgt catcgcctcg atccggatgt cgtaaaccgt taccccgccc ggccattcgg tcatcctggt ggccatcggc gcgcccatat tgcccagccc gatgtagccg agcttcagct gctcggtcgt gtgcgccgcg ttcgtcgtca tcgaaccagg accctgggtc tgagcttccn ngnncccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTNAAHTT EQLKLGYIGL GNMGAPMATR MTEWPGGVTV YDIRIEAMTP LAEAGATLAD SVADVAAADL IHITVLDDAQ VREVVGELAG HAKPGTVIAI HSTISDTTAV ELARDLKARD IHIVDAPVSG GAAAAARGEL ATMVGADREV YERIKPAFKH WAAVVIHAGE PGAGTRMKLA RNMLTFTSYA AACEAMKLAE AAGLDLQALG RVVRHTDALT GGPGAIMVRD NMKDLEPDNF LYQPFLHTRG LGEKDLSLAL ALGEAVSVDL PLARLAYEGL AAGLGVPHKE KEA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgacgaac gcggcgcaca cgaccgagca gctgaagctc ggctacatcg ggctgggcaa tatgggcgcg ccgatggcca ccaggatgac cgaatggccg ggcggggtaa cggtttacga catccggatc gaggcgatga cgccgctggc cgaggccggc gccacccttg ccgacagcgt ggccgacgtc gccgccgccg acctgatcca catcaccgtg ctcgacgacg cccaggtgcg tgaggtggtc ggcgagctgg ccgggcacgc caagcccggc acggtgatcg cgatccactc gaccatcagc gacaccaccg ccgtcgaact ggcccgcgat ctcaaggcgc gggacatcca catcgtcgac gccccggtca gcggcggcgc ggccgccgcc gcccggggtg agctcgccac catggtgggc gccgaccgcg aggtctacga gcggatcaag ccggcgttca aacactgggc ggcggtggtc atccacgccg gtgaaccggg tgcgggaacc cgaatgaagt tggcccgcaa catgttgacg ttcacctcgt atgccgcagc gtgcgaggcg atgaagttgg ccgaggcggc cgggctggac ctgcaggcgc tgggccgggt ggtgcgtcat accgacgcgc tcaccggcgg tccgggcgcg atcatggtgc gcgacaacat gaaagacctc gagccggaca acttcctcta ccagccgttc ttgcacaccc gcggcctggg ggagaaggat ctgagcctgg cgctggcgtt gggtgaggcg gtctcggtcg acctgccgct ggcccggctg gcgtacgagg gactggcggc cgggctcggc gtgccacaca aggagaaaga ggcgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MyavA.01379.a.A1.PS00711
| PURIFICATION DATe: | 8/5/2010 |
| CONCENTRATION: | 22.39mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 92 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTTNAAHTTE QLKLGYIGLG NMGAPMATRM TEWPGGVTVY DIRIEAMTPL AEAGATLADS VADVAAADLI HITVLDDAQV REVVGELAGH AKPGTVIAIH STISDTTAVE LARDLKARDI HIVDAPVSGG AAAAARGELA TMVGADREVY ERIKPAFKHW AAVVIHAGEP GAGTRMKLAR NMLTFTSYAA ACEAMKLAEA AGLDLQALGR VVRHTDALTG GPGAIMVRDN MKDLEPDNFL YQPFLHTRGL GEKDLSLALA LGEAVSVDLP LARLAYEGLA AGLGVPHKEK
Validated NT Sequence
ctttctcctt gtgtggcacg ccgagcccgg ccgccagtcc ctcgtacgcc agccgggcca gcggcaggtc gaccgagacc gcctcaccca acgccagcgc caggctcaga tccttctccc ccaggccgcg ggtgtgcaag aacggctggt agaggaagtt gtccggctcg aggtctttca tgttgtcgcg caccatgatc gcgcccggac cgccggtgag cgcgtcggta tgacgcacca cccggcccag cgcctgcagg tccagcccgg ccgcctcggc caacttcatc gcctcgcacg ctgcggcata cgaggtgaac gtcaacatgt tgcgggccaa cttcattcgg gttcccgcac ccggttcacc ggcgtggatg accaccgccg cccagtgttt gaacgccggc ttgatccgct cgtagacctc gcggtcggcg cccaccatgg tggcgagctc accccgggcg gcggcggccg cgccgccgct gaccggggcg tcgacgatgt ggatgtcccg cgccttgaga tcgcgggcca gttcgacggc ggtggtgtcg ctgatggtcg agtggatcgc gatcaccgtg ccgggcttgg cgtgcccggc cagctcgccg accacctcac gcacctgggc gtcgtcgagc acggtgatgt ggatcaggtc ggcggcggcg acgtcggcca cgctgtcggc aagggtggcg ccggcctcgg ccagcggcgt catcgcctcg atccggatgt cgtaaaccgt taccccgccc ggccattcgg tcatcctggt ggccatcggc gcgcccatat tgcccagccc gatgtagccg agcttcagct gctcggtcgt gtgcgccgcg ttcgtcgtca tcgaaccagg accctgggtc tgagcttccn ngnncccata tgatggtgat ggtgatgagc cat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTNAAHTT EQLKLGYIGL GNMGAPMATR MTEWPGGVTV YDIRIEAMTP LAEAGATLAD SVADVAAADL IHITVLDDAQ VREVVGELAG HAKPGTVIAI HSTISDTTAV ELARDLKARD IHIVDAPVSG GAAAAARGEL ATMVGADREV YERIKPAFKH WAAVVIHAGE PGAGTRMKLA RNMLTFTSYA AACEAMKLAE AAGLDLQALG RVVRHTDALT GGPGAIMVRD NMKDLEPDNF LYQPFLHTRG LGEKDLSLAL ALGEAVSVDL PLARLAYEGL AAGLGVPHKE KEA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGACGAAC GCGGCGCACA CGACCGAGCA GCTGAAGCTC GGCTACATCG GGCTGGGCAA TATGGGCGCG CCGATGGCCA CCAGGATGAC CGAATGGCCG GGCGGGGTAA CGGTTTACGA CATCCGGATC GAGGCGATGA CGCCGCTGGC CGAGGCCGGC GCCACCCTTG CCGACAGCGT GGCCGACGTC GCCGCCGCCG ACCTGATCCA CATCACCGTG CTCGACGACG CCCAGGTGCG TGAGGTGGTC GGCGAGCTGG CCGGGCACGC CAAGCCCGGC ACGGTGATCG CGATCCACTC GACCATCAGC GACACCACCG CCGTCGAACT GGCCCGCGAT CTCAAGGCGC GGGACATCCA CATCGTCGAC GCCCCGGTCA GCGGCGGCGC GGCCGCCGCC GCCCGGGGTG AGCTCGCCAC CATGGTGGGC GCCGACCGCG AGGTCTACGA GCGGATCAAG CCGGCGTTCA AACACTGGGC GGCGGTGGTC ATCCACGCCG GTGAACCGGG TGCGGGAACC CGAATGAAGT TGGCCCGCAA CATGTTGACG TTCACCTCGT ATGCCGCAGC GTGCGAGGCG ATGAAGTTGG CCGAGGCGGC CGGGCTGGAC CTGCAGGCGC TGGGCCGGGT GGTGCGTCAT ACCGACGCGC TCACCGGCGG TCCGGGCGCG ATCATGGTGC GCGACAACAT GAAAGACCTC GAGCCGGACA ACTTCCTCTA CCAGCCGTTC TTGCACACCC GCGGCCTGGG GGAGAAGGAT CTGAGCCTGG CGCTGGCGTT GGGTGAGGCG GTCTCGGTCG ACCTGCCGCT GGCCCGGCTG GCGTACGAGG GACTGGCGGC CGGGCTCGGC GTGCCACACA AGGAGAAAGA GGCGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA