MyavA.01365.a

Short chain dehydrogenase

CENTER ID: MyavA.01365.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01365.a.A1.GE29116 Full length( MyavA.01365.a ) 1 275
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01365.a.A1.PW29021 Full length( MyavA.01365.a ) 1 275

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.5145
RefSeq: YP_884354.1
UniProt: A0A0H3A3U4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVITGGAGG MGVATAKVVG RDHTLVLCDV RQDRLTAATA ALAELGMTPT AVNCDVTDRR AVAQLFDTAA GLGTVVSVIH TAGVSPSMGP ADYVMRTNAI GTLNVNEAFY AVAGDGSVIV NVASMAAHML PAEIVPTAQF PLALSDADAF MDAMLAACEI APEQARSGLA YAVSKSFVKW YSQSQAERFN ARGLRIVSVS PGSIDTEMGR LEENAGAGAM VADAAVPRWG KPEEMAELLA FCASDKAGYL TGTDILNDGG VIASMTERAR VTAAG
NT Sequence
GTGTCGGTGA TCACAGGCGG CGCGGGCGGC ATGGGAGTGG CCACCGCGAA AGTCGTCGGC CGCGATCACA CCCTGGTGCT GTGCGACGTC CGGCAGGACC GGTTGACGGC CGCGACCGCC GCGCTGGCAG AGCTCGGGAT GACGCCCACC GCGGTCAACT GCGACGTCAC CGATCGGCGG GCCGTCGCGC AGCTGTTCGA CACCGCGGCC GGGCTCGGCA CCGTCGTCTC CGTCATCCAC ACCGCCGGGG TGAGCCCGAG CATGGGCCCG GCCGACTACG TGATGCGGAC CAACGCGATC GGCACCCTCA ACGTCAACGA GGCGTTCTAC GCGGTCGCCG GCGACGGCTC GGTGATCGTC AACGTCGCGT CGATGGCGGC GCACATGCTG CCCGCGGAAA TCGTTCCGAC AGCGCAGTTT CCGCTCGCGC TTAGCGACGC CGACGCCTTC ATGGACGCCA TGCTGGCGGC GTGCGAGATC GCGCCCGAGC AGGCCCGCTC GGGGCTGGCC TACGCCGTGA GCAAGAGCTT CGTCAAGTGG TACAGCCAGT CACAGGCCGA GCGCTTCAAC GCGCGGGGCC TGCGCATCGT CTCGGTGTCC CCCGGGTCGA TCGACACCGA AATGGGCCGG CTCGAGGAGA ACGCCGGCGC CGGGGCGATG GTGGCCGACG CCGCGGTCCC GCGGTGGGGC AAGCCCGAGG AGATGGCCGA GCTTCTGGCC TTCTGCGCCA GCGACAAGGC CGGATACCTC ACCGGCACGG ACATTCTCAA CGACGGCGGG GTGATCGCCT CGATGACCGA GCGGGCCAGG GTGACCGCCG CCGGC
Details for MyavA.01365.a.A1.GE29116
HARVESTED ON: 4/27/2010
SEQUENCED ON: 5/1/2010
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSVITGGA GGMGVATAKV VGRDHTLVLC DVRQDRLTAA TAALAELGMT PTAVNCDVTD RRAVAQLFDT AAGLGTVVSV IHTAGVSPSM GPADYVMRTN AIGTLNVNEA FYAVAGDGSV IVNVASMAAH MLPAEIVPTA QFPLALSDAD AFMDAMLAAC EIAPEQARSG LAYAVSKSFV KWYSQSQAER FNARGLRIVS VSPGSIDTEM GRLEENAGAG AMVADAAVPR WGKPEEMAEL LAFCASDKAG YLTGTDILND GGVIASMTER ARVTAAG
Validated NT Sequence
ttattagccg gcggcggtca ccctggcccg ctcggtcatc gaggcgatca ccccgccgtc gttgagaatg tccgtgccgg tgaggtatcc ggccttgtcg ctggcgcaga aggccagaag ctcggccatc tcctcgggct tgccccaccg cgggaccgcg gcgtcggcca ccatcgcccc ggcgccggcg ttctcctcga gccggcccat ttcggtgtcg atcgacccgg gggacaccga gacgatgcgc aggccccgcg cgttgaagcg ctcggcctgt gactggctgt accacttgac gaagctcttg ctcacggcgt aggccagccc cgagcgggcc tgctcgggcg cgatctcgca cgccgccagc atggcgtcca tgaaggcgtc ggcgtcgcta agcgcgagcg gaaactgcgc tgtcggaacg atttccgcgg gcagcatgtg cgccgccatc gacgcgacgt tgacgatcac cgagccgtcg ccggcgaccg cgtagaacgc ctcgttgacg ttgagggtgc cgatcgcgtt ggtccgcatc acgtagtcgg ccgggcccat gctcgggctc accccggcgg tgtggatgac ggagacgacg gtgccgagcc cggccgcggt gtcgaacagc tgcgcgacgg cccgccgatc ggtgacgtcg cagttgaccg cggtgggcgt catcccgagc tctgccagcg cggcggtcgc ggccgtcaac cggtcctgcc ggacgtcgca cagcaccagg gtgtgatcgc ggccgacgac tttcgcggtg gccactccca tgccgcccgc gccgcctgtg atcaccgaca ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccatggt at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSVITGGA GGMGVATAKV VGRDHTLVLC DVRQDRLTAA TAALAELGMT PTAVNCDVTD RRAVAQLFDT AAGLGTVVSV IHTAGVSPSM GPADYVMRTN AIGTLNVNEA FYAVAGDGSV IVNVASMAAH MLPAEIVPTA QFPLALSDAD AFMDAMLAAC EIAPEQARSG LAYAVSKSFV KWYSQSQAER FNARGLRIVS VSPGSIDTEM GRLEENAGAG AMVADAAVPR WGKPEEMAEL LAFCASDKAG YLTGTDILND GGVIASMTER ARVTAAG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtcggtg atcacaggcg gcgcgggcgg catgggagtg gccaccgcga aagtcgtcgg ccgcgatcac accctggtgc tgtgcgacgt ccggcaggac cggttgacgg ccgcgaccgc cgcgctggca gagctcggga tgacgcccac cgcggtcaac tgcgacgtca ccgatcggcg ggccgtcgcg cagctgttcg acaccgcggc cgggctcggc accgtcgtct ccgtcatcca caccgccggg gtgagcccga gcatgggccc ggccgactac gtgatgcgga ccaacgcgat cggcaccctc aacgtcaacg aggcgttcta cgcggtcgcc ggcgacggct cggtgatcgt caacgtcgcg tcgatggcgg cgcacatgct gcccgcggaa atcgttccga cagcgcagtt tccgctcgcg cttagcgacg ccgacgcctt catggacgcc atgctggcgg cgtgcgagat cgcgcccgag caggcccgct cggggctggc ctacgccgtg agcaagagct tcgtcaagtg gtacagccag tcacaggccg agcgcttcaa cgcgcggggc ctgcgcatcg tctcggtgtc ccccgggtcg atcgacaccg aaatgggccg gctcgaggag aacgccggcg ccggggcgat ggtggccgac gccgcggtcc cgcggtgggg caagcccgag gagatggccg agcttctggc cttctgcgcc agcgacaagg ccggatacct caccggcacg gacattctca acgacggcgg ggtgatcgcc tcgatgaccg agcgggccag ggtgaccgcc gccggcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MyavA.01365.a.A1.PW29021
PURIFICATION DATe: 1/21/2011
CONCENTRATION: 29.48mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSVITGGA GGMGVATAKV VGRDHTLVLC DVRQDRLTAA TAALAELGMT PTAVNCDVTD RRAVAQLFDT AAGLGTVVSV IHTAGVSPSM GPADYVMRTN AIGTLNVNEA FYAVAGDGSV IVNVASMAAH MLPAEIVPTA QFPLALSDAD AFMDAMLAAC EIAPEQARSG LAYAVSKSFV KWYSQSQAER FNARGLRIVS VSPGSIDTEM GRLEENAGAG AMVADAAVPR WGKPEEMAEL LAFCASDKAG YLTGTDILND GGVIASMTER ARVTAAG
Validated NT Sequence
ttattagccg gcggcggtca ccctggcccg ctcggtcatc gaggcgatca ccccgccgtc gttgagaatg tccgtgccgg tgaggtatcc ggccttgtcg ctggcgcaga aggccagaag ctcggccatc tcctcgggct tgccccaccg cgggaccgcg gcgtcggcca ccatcgcccc ggcgccggcg ttctcctcga gccggcccat ttcggtgtcg atcgacccgg gggacaccga gacgatgcgc aggccccgcg cgttgaagcg ctcggcctgt gactggctgt accacttgac gaagctcttg ctcacggcgt aggccagccc cgagcgggcc tgctcgggcg cgatctcgca cgccgccagc atggcgtcca tgaaggcgtc ggcgtcgcta agcgcgagcg gaaactgcgc tgtcggaacg atttccgcgg gcagcatgtg cgccgccatc gacgcgacgt tgacgatcac cgagccgtcg ccggcgaccg cgtagaacgc ctcgttgacg ttgagggtgc cgatcgcgtt ggtccgcatc acgtagtcgg ccgggcccat gctcgggctc accccggcgg tgtggatgac ggagacgacg gtgccgagcc cggccgcggt gtcgaacagc tgcgcgacgg cccgccgatc ggtgacgtcg cagttgaccg cggtgggcgt catcccgagc tctgccagcg cggcggtcgc ggccgtcaac cggtcctgcc ggacgtcgca cagcaccagg gtgtgatcgc ggccgacgac tttcgcggtg gccactccca tgccgcccgc gccgcctgtg atcaccgaca ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccatggt at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSVITGGA GGMGVATAKV VGRDHTLVLC DVRQDRLTAA TAALAELGMT PTAVNCDVTD RRAVAQLFDT AAGLGTVVSV IHTAGVSPSM GPADYVMRTN AIGTLNVNEA FYAVAGDGSV IVNVASMAAH MLPAEIVPTA QFPLALSDAD AFMDAMLAAC EIAPEQARSG LAYAVSKSFV KWYSQSQAER FNARGLRIVS VSPGSIDTEM GRLEENAGAG AMVADAAVPR WGKPEEMAEL LAFCASDKAG YLTGTDILND GGVIASMTER ARVTAAG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTCGGTG ATCACAGGCG GCGCGGGCGG CATGGGAGTG GCCACCGCGA AAGTCGTCGG CCGCGATCAC ACCCTGGTGC TGTGCGACGT CCGGCAGGAC CGGTTGACGG CCGCGACCGC CGCGCTGGCA GAGCTCGGGA TGACGCCCAC CGCGGTCAAC TGCGACGTCA CCGATCGGCG GGCCGTCGCG CAGCTGTTCG ACACCGCGGC CGGGCTCGGC ACCGTCGTCT CCGTCATCCA CACCGCCGGG GTGAGCCCGA GCATGGGCCC GGCCGACTAC GTGATGCGGA CCAACGCGAT CGGCACCCTC AACGTCAACG AGGCGTTCTA CGCGGTCGCC GGCGACGGCT CGGTGATCGT CAACGTCGCG TCGATGGCGG CGCACATGCT GCCCGCGGAA ATCGTTCCGA CAGCGCAGTT TCCGCTCGCG CTTAGCGACG CCGACGCCTT CATGGACGCC ATGCTGGCGG CGTGCGAGAT CGCGCCCGAG CAGGCCCGCT CGGGGCTGGC CTACGCCGTG AGCAAGAGCT TCGTCAAGTG GTACAGCCAG TCACAGGCCG AGCGCTTCAA CGCGCGGGGC CTGCGCATCG TCTCGGTGTC CCCCGGGTCG ATCGACACCG AAATGGGCCG GCTCGAGGAG AACGCCGGCG CCGGGGCGAT GGTGGCCGAC GCCGCGGTCC CGCGGTGGGG CAAGCCCGAG GAGATGGCCG AGCTTCTGGC CTTCTGCGCC AGCGACAAGG CCGGATACCT CACCGGCACG GACATTCTCA ACGACGGCGG GGTGATCGCC TCGATGACCG AGCGGGCCAG GGTGACCGCC GCCGGCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA