SSGCID: Target Page

MyavA.01285.a

Methylthioadenosine phosphorylase

CENTER ID:	MyavA.01285.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01285.a.A2.GE34917	Mature protein( MyavA.01285.a )		24	264

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.4526
RefSeq:	YP_883739.1
UniProt:	A0A0H2ZYD9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHHNGRMLGV IGGSGFYIFF GSDADDVTVD TPYGPPSAPV TVGAVGGHRV AFLPRHGARH EFSAHTVPYR ANLWALRKLG VRRVLAPCAV GSLVPELGPG AVVVPDQLVD RTRGRADTYF DSGAVHVDFA DPYCPALREA VTGLPGVVDG GTMVVIQGPR FSTRAESRWF ASAGFSLVNM TGYPEAVLAR ELEMCYAAIA LVTDLDAGVS AGEGVKAVEV FAEFEKNIEP FKKLVRDAIG RVAAERSCTQ CSPHAGVSLP IELP

NT Sequence

ATGCACCACA ATGGTCGGAT GCTCGGAGTC ATCGGCGGCA GCGGCTTCTA CATCTTCTTC GGATCCGACG CCGACGACGT CACCGTCGAT ACCCCGTACG GGCCGCCCAG CGCCCCGGTC ACCGTCGGCG CCGTCGGCGG GCACCGGGTG GCCTTCCTGC CCCGGCACGG CGCCCGGCAC GAATTCTCCG CGCACACCGT GCCGTACCGG GCCAACCTGT GGGCGCTGCG CAAACTCGGT GTGCGCCGGG TGCTGGCGCC GTGCGCGGTC GGCAGCCTGG TGCCCGAGCT CGGCCCGGGC GCCGTCGTGG TGCCCGACCA GCTGGTCGAC CGCACCCGCG GGCGCGCCGA CACCTATTTC GACTCCGGGG CCGTGCACGT CGACTTCGCC GACCCGTACT GCCCGGCGCT GCGCGAGGCG GTGACCGGGC TGCCGGGGGT GGTCGATGGC GGCACCATGG TGGTGATCCA GGGGCCGCGG TTTTCCACCC GCGCCGAGAG CCGGTGGTTC GCCTCGGCCG GGTTCTCGCT GGTGAACATG ACCGGCTACC CGGAGGCGGT GCTCGCCCGG GAACTGGAAA TGTGCTATGC GGCAATCGCT TTGGTCACGG ATCTGGACGC CGGTGTGAGC GCCGGTGAGG GAGTGAAGGC GGTTGAGGTG TTCGCCGAGT TCGAGAAGAA CATCGAGCCG TTCAAGAAGC TGGTGCGCGA CGCCATCGGC CGGGTCGCCG CCGAGCGCAG CTGTACGCAG TGCTCACCGC ACGCCGGGGT GAGCCTGCCG ATCGAGCTGC CA

Details for MyavA.01285.a.A2.GE34917

HARVESTED ON:	4/17/2012
SEQUENCED ON:	4/17/2012
EXPECTED MW:	27kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMADDVTVDT PYGPPSAPVT VGAVGGHRVA FLPRHGARHE FSAHTVPYRA NLWALRKLGV RRVLAPCAVG SLVPELGPGA VVVPDQLVDR TRGRADTYFD SGAVHVDFAD PYCPALREAV TGLPGVVDGG TMVVIQGPRF STRAESRWFA SAGFSLVNMT GYPEAVLARE LEMCYAAIAL VTDLDAGVSA GEGVKAVEVF AEFEKNIEPF KKLVRDAXGR VAAERSCTQC SPHAGVSLPI ELP

Validated NT Sequence

ttttgnttaa ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccg acgacgtcac cgtcgatacc ccgtacgggc cgcccagcgc cccggtcacc gtcggcgccg tcggcgggca ccgggtggcc ttcctgcccc ggcacggcgc ccggcacgaa ttctccgcgc acaccgtgcc gtaccgggcc aacctgtggg cgctgcgcaa actcggtgtg cgccgggtgc tggcgccgtg cgcggtcggc agcctggtgc ccgagctcgg cccgggcgcc gtcgtggtgc ccgaccagct ggtcgaccgc acccgcgggc gcgccgacac ctatttcgac tccggggccg tgcacgtcga cttcgccgac ccgtactgcc cggcgctgcg cgaggcggtg accgggctgc cgggggtggt cgatggcggc accatggtgg tgatccaggg gccgcggttt tccacccgcg ccgagagccg gtggttcgcc tcggccgggt tctcgctggt gaacatgacc ggctacccgg aggcggtgct cgcccgggaa ctggaaatgt gctatgcggc aatcgctttg gtcacggatc tggacgccgg tgtgagcgcc ggtgagggag tgaaggcggt tgaggtgttc gccgagttcg agaagaacat cgagccgttc aagaagctgg tgcgcgacgc cntcggccgg gtcgccgccg agcgcagctg tacgcagtgc tcaccgcacg ccggggtgag cctgccgatc gagctgccat aataaacagc acgaacaagt tctgcagcca agcttctcga ggancnnnnn nnnnnnncnn nnnnnnnnnn nnnnnnnnnn nntnnggntc tgcttncnnn anannnnnnn nnnnnnannn ng

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgacgac gtcaccgtcg ataccccgta cgggccgccc agcgccccgg tcaccgtcgg cgccgtcggc gggcaccggg tggccttcct gccccggcac ggcgcccggc acgaattctc cgcgcacacc gtgccgtacc gggccaacct gtgggcgctg cgcaaactcg gtgtgcgccg ggtgctggcg ccgtgcgcgg tcggcagcct ggtgcccgag ctcggcccgg gcgccgtcgt ggtgcccgac cagctggtcg accgcacccg cgggcgcgcc gacacctatt tcgactccgg ggccgtgcac gtcgacttcg ccgacccgta ctgcccggcg ctgcgcgagg cggtgaccgg gctgccgggg gtggtcgatg gcggcaccat ggtggtgatc caggggccgc ggttttccac ccgcgccgag agccggtggt tcgcctcggc cgggttctcg ctggtgaaca tgaccggcta cccggaggcg gtgctcgccc gggaactgga aatgtgctat gcggcaatcg ctttggtcac ggatctggac gccggtgtga gcgccggtga gggagtgaag gcggttgagg tgttcgccga gttcgagaag aacatcgagc cgttcaagaa gctggtgcgc gacgccatcg gccgggtcgc cgccgagcgc agctgtacgc agtgctcacc gcacgccggg gtgagcctgc cgatcgagct gccaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa