SSGCID: Target Page

MyavA.01249.c

Retinol dehydrogenase 13

CENTER ID:	MyavA.01249.c
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01249.c.A1.GE29082	Full length( MyavA.01249.c )		1	289

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01249.c.A1.PS00741	Full length( MyavA.01249.c )		1	289

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.1126
RefSeq:	YP_880403.1
UniProt:	A0A0H2ZVV5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTGWTAADLP SFAQRTVVIT GANSGLGAVT ARELARRGAT VIMAVRDTRK GEAAARTMAG QVEVRELDLQ DLSSVRRFAD GVSGADVLIN NAGIMAVPYA LTVDGFESQI GTNHLGHFAL TNLLLPRLTD RVVTVSSMAH WPGRINLEDL NWRSRRYSPW LAYSQSKLAN LLFTSELQRR LTAAGSPLRA LAAHPGYSHT NLQGASGRKL GDALMSAATR VVATDADFGA RQTLYAASQD LTGDTFVGPR FGYLGRTQPV GRSRRAKDAG MAAALWALSE QLTKTEFPL

NT Sequence

ATGACAGGCT GGACCGCCGC AGACCTACCG TCGTTCGCGC AGCGAACCGT CGTCATCACC GGCGCCAACA GCGGGTTGGG TGCGGTGACC GCCCGCGAGC TGGCGCGACG GGGGGCCACC GTCATCATGG CCGTGCGCGA CACCCGCAAG GGCGAGGCGG CCGCGCGGAC GATGGCCGGC CAGGTCGAGG TCCGCGAACT CGACCTGCAG GATCTGTCGT CGGTGCGCCG GTTCGCCGAC GGGGTGAGCG GGGCCGACGT GCTGATCAAC AACGCCGGCA TCATGGCCGT CCCGTACGCG CTGACCGTCG ACGGCTTCGA GAGCCAGATC GGCACCAACC ACCTGGGCCA TTTCGCGCTG ACCAACCTGC TGCTGCCCCG GCTCACCGAC CGCGTCGTGA CGGTGTCGTC GATGGCGCAC TGGCCGGGAC GGATCAACCT CGAGGACCTG AACTGGCGGT CGCGCCGGTA CTCGCCGTGG CTGGCCTACA GCCAGTCCAA GCTGGCCAAC CTGCTGTTCA CCAGCGAGCT GCAGCGCCGC CTGACGGCCG CCGGTTCCCC GCTGCGCGCG CTGGCCGCCC ACCCCGGCTA CTCGCACACC AACCTGCAGG GCGCCTCGGG CCGCAAGCTG GGCGACGCGC TGATGTCGGC GGCGACGCGG GTCGTCGCCA CCGACGCCGA TTTCGGCGCC CGGCAAACCC TGTACGCGGC GTCCCAGGAT CTGACCGGGG ACACCTTCGT CGGCCCCCGC TTCGGTTACC TGGGCCGCAC CCAACCGGTC GGACGCAGCC GGCGGGCCAA AGATGCCGGC ATGGCGGCGG CGCTGTGGGC GCTCTCCGAG CAGCTCACCA AGACCGAATT TCCGCTC

Details for MyavA.01249.c.A1.GE29082

HARVESTED ON:	4/9/2010
SEQUENCED ON:	4/29/2010
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMTGWTAA DLPSFAQRTV VITGANSGLG AVTARELARR GATVIMAVRD TRKGEAAART MAGQVEVREL DLQDLSSVRR FADGVSGADV LINNAGIMAV PYALTVDGFE SQIGTNHLGH FALTNLLLPR LTDRVVTVSS MAHWPGRINL EDLNWRSRRY SPWLAYSQSK LANLLFTSEL QRRLTAAGSP LRALAAHPGY SHTNLQGASG RKLGDALMSA ATRVVATDAD FGARQTLYAA SQDLPGDTFV GPRFGYLGRT QPVGRSRRAK DAGMAAALWA LSEQLTKTEF PL

Validated NT Sequence

ttattagagc ggaaattcgg tcttggtgag ctgctcggag agcgcccaca gcgccgccgc catgccggca tctttggccc gccggctgcg tccgaccggt tgggtgcggc ccaggtaacc gaagcggggg ccgacgaagg tgtccccggg cagatcctgg gacgccgcgt acagggtttg ccgggcgccg aaatcggcgt cggtggcgac gacccgcgtc gccgccgaca tcagcgcgtc gcccagcttg cggcccgagg cgccctgcag gttggtgtgc gagtagccgg ggtgggcggc cagcgcgcgc agcggggaac cggcggccgt caggcggcgc tgcagctcgc tggtgaacag caggttggcc agcttggact ggctgtaggc cagccacggc gagtaccggc gcgaccgcca gttcaggtcc tcgaggttga tccgtcccgg ccagtgcgcc atcgacgaca ccgtcacgac gcggtcggtg agccggggca gcagcaggtt ggtcagcgcg aaatggccca ggtggttggt gccgatctgg ctctcgaagc cgtcgacggt cagcgcgtac gggacggcca tgatgccggc gttgttgatc agcacgtcgg ccccgctcac cccgtcggcg aaccggcgca ccgacgacag atcctgcagg tcgagttcgc ggacctcgac ctggccggcc atcgtccgcg cggccgcctc gcccttgcgg gtgtcgcgca cggccatgat gacggtggcc ccccgtcgcg ccagctcgcg ggcggtcacc gcacccaacc cgctgttggc gccggtgatg acgacggttc gctgcgcgaa cgacggtagg tctgcggcgg tccagcctgt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agcca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTGWTAADL PSFAQRTVVI TGANSGLGAV TARELARRGA TVIMAVRDTR KGEAAARTMA GQVEVRELDL QDLSSVRRFA DGVSGADVLI NNAGIMAVPY ALTVDGFESQ IGTNHLGHFA LTNLLLPRLT DRVVTVSSMA HWPGRINLED LNWRSRRYSP WLAYSQSKLA NLLFTSELQR RLTAAGSPLR ALAAHPGYSH TNLQGASGRK LGDALMSAAT RVVATDADFG ARQTLYAASQ DLTGDTFVGP RFGYLGRTQP VGRSRRAKDA GMAAALWALS EQLTKTEFPL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaggctgg accgccgcag acctaccgtc gttcgcgcag cgaaccgtcg tcatcaccgg cgccaacagc gggttgggtg cggtgaccgc ccgcgagctg gcgcgacggg gggccaccgt catcatggcc gtgcgcgaca cccgcaaggg cgaggcggcc gcgcggacga tggccggcca ggtcgaggtc cgcgaactcg acctgcagga tctgtcgtcg gtgcgccggt tcgccgacgg ggtgagcggg gccgacgtgc tgatcaacaa cgccggcatc atggccgtcc cgtacgcgct gaccgtcgac ggcttcgaga gccagatcgg caccaaccac ctgggccatt tcgcgctgac caacctgctg ctgccccggc tcaccgaccg cgtcgtgacg gtgtcgtcga tggcgcactg gccgggacgg atcaacctcg aggacctgaa ctggcggtcg cgccggtact cgccgtggct ggcctacagc cagtccaagc tggccaacct gctgttcacc agcgagctgc agcgccgcct gacggccgcc ggttccccgc tgcgcgcgct ggccgcccac cccggctact cgcacaccaa cctgcagggc gcctcgggcc gcaagctggg cgacgcgctg atgtcggcgg cgacgcgggt cgtcgccacc gacgccgatt tcggcgcccg gcaaaccctg tacgcggcgt cccaggatct gaccggggac accttcgtcg gcccccgctt cggttacctg ggccgcaccc aaccggtcgg acgcagccgg cgggccaaag atgccggcat ggcggcggcg ctgtgggcgc tctccgagca gctcaccaag accgaatttc cgctcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for MyavA.01249.c.A1.PS00741

PURIFICATION DATe:	8/27/2010
CONCENTRATION:	12.06mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAGGCTGG ACCGCCGCAG ACCTACCGTC GTTCGCGCAG CGAACCGTCG TCATCACCGG CGCCAACAGC GGGTTGGGTG CGGTGACCGC CCGCGAGCTG GCGCGACGGG GGGCCACCGT CATCATGGCC GTGCGCGACA CCCGCAAGGG CGAGGCGGCC GCGCGGACGA TGGCCGGCCA GGTCGAGGTC CGCGAACTCG ACCTGCAGGA TCTGTCGTCG GTGCGCCGGT TCGCCGACGG GGTGAGCGGG GCCGACGTGC TGATCAACAA CGCCGGCATC ATGGCCGTCC CGTACGCGCT GACCGTCGAC GGCTTCGAGA GCCAGATCGG CACCAACCAC CTGGGCCATT TCGCGCTGAC CAACCTGCTG CTGCCCCGGC TCACCGACCG CGTCGTGACG GTGTCGTCGA TGGCGCACTG GCCGGGACGG ATCAACCTCG AGGACCTGAA CTGGCGGTCG CGCCGGTACT CGCCGTGGCT GGCCTACAGC CAGTCCAAGC TGGCCAACCT GCTGTTCACC AGCGAGCTGC AGCGCCGCCT GACGGCCGCC GGTTCCCCGC TGCGCGCGCT GGCCGCCCAC CCCGGCTACT CGCACACCAA CCTGCAGGGC GCCTCGGGCC GCAAGCTGGG CGACGCGCTG ATGTCGGCGG CGACGCGGGT CGTCGCCACC GACGCCGATT TCGGCGCCCG GCAAACCCTG TACGCGGCGT CCCAGGATCT GACCGGGGAC ACCTTCGTCG GCCCCCGCTT CGGTTACCTG GGCCGCACCC AACCGGTCGG ACGCAGCCGG CGGGCCAAAG ATGCCGGCAT GGCGGCGGCG CTGTGGGCGC TCTCCGAGCA GCTCACCAAG ACCGAATTTC CGCTCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA