SSGCID: Target Page

MyavA.01204.c

Short-chain dehydrogenase/reductase SDR

CENTER ID:	MyavA.01204.c
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01204.c.A1.GE29077	Full length( MyavA.01204.c )		1	288

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01204.c.A1.PS00831	Full length( MyavA.01204.c )		1	288

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.4895
RefSeq:	YP_884103.1
UniProt:	A0A0H2ZXX8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPKTWFITGS SRGLGRALTT AVLDHGDHVV ATARRPAQLD ELVDRYGPRI RACELDVTDR AAARRAIAAA VTAFGRIDVV VNNAGYANSA PVEEVSDDDF RAQVETNFFG VVNVTKAALP VLHRQRAGHI VQISSVGGRV GGSPGIAAYQ ASKFAVAGFS EALAAEVAPL GIKVTIAEPG ALRTEWAGSS MAVATVSPDY ESTVGAMNRR RAGFNRNAPG DPARAAHAII EVVIADDPPL RLLLGSDALS AALAKSHQQI AEANRWAELS KSIDYPPVAT PHRAARSR

NT Sequence

ATGCCGAAGA CGTGGTTCAT CACCGGCAGC TCGCGCGGGC TCGGCCGCGC CCTGACGACC GCGGTGCTCG ACCATGGCGA CCATGTCGTC GCGACCGCCC GTCGCCCCGC ACAGCTCGAT GAACTCGTCG ACCGGTACGG CCCCCGGATC CGGGCCTGTG AGCTCGACGT CACCGATCGA GCTGCCGCGC GGCGTGCCAT CGCGGCCGCC GTCACGGCGT TCGGCCGGAT CGACGTCGTG GTGAACAACG CCGGTTACGC CAATTCCGCA CCCGTGGAGG AGGTTTCCGA CGACGATTTC CGCGCGCAGG TGGAAACCAA CTTCTTCGGT GTCGTGAACG TCACGAAAGC CGCGCTCCCC GTGCTGCACC GGCAACGGGC CGGCCACATC GTGCAGATCT CCTCGGTTGG CGGCCGCGTT GGTGGATCCC CGGGCATCGC CGCGTACCAG GCGTCCAAGT TCGCCGTCGC AGGGTTCTCG GAGGCACTCG CCGCGGAGGT AGCTCCGCTC GGTATCAAGG TGACGATCGC CGAACCGGGG GCCTTGCGCA CGGAATGGGC CGGCTCGTCG ATGGCCGTCG CGACCGTCTC GCCGGACTAC GAAAGCACCG TCGGTGCGAT GAACCGCCGC CGCGCCGGTT TCAACCGCAA CGCTCCGGGC GATCCCGCCC GCGCCGCACA CGCGATCATC GAGGTGGTTA TTGCCGACGA TCCGCCACTG CGGCTGCTAC TCGGCTCCGA TGCGCTCAGT GCCGCGCTGG CCAAGTCACA TCAGCAGATT GCCGAAGCGA ACCGGTGGGC GGAGTTGAGT AAATCCATCG ACTATCCGCC CGTCGCCACC CCACACCGAG CTGCGCGCAG TCGA

Details for MyavA.01204.c.A1.GE29077

HARVESTED ON:	4/9/2010
SEQUENCED ON:	4/29/2010
EXPECTED MW:	32kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPKTWFITG SSRGLGRALT TAVLDRGDQV VATARRPAQL DELVDRYGPR IRACELDVTD RAAARRAIAA AVTAFGRIDV VVNNAGYANS APVEEVSDDD FRAQVETNFF GVVNVTKAAL SVLHRQRAGH IVQISSVGGR VGGSPGIAAY QASKFAVAGF SEALAAEVAP LGIKVTIAEP GALRTEWAGS SMAVATVSPD YESTVGAMNR RRAGFNRNAP GDPARAAHAI IEVVMADDPP LRLLLGSDAL SAALAKSHQQ IAEANRWAEL SKSVDYPPVA TPHRAARSR

Validated NT Sequence

ttattatcga ctgcgcgcag ctcggtgagg ggtggcgacg ggcggatagt ctacggattt actcaactcc gcccaccggt tcgcctcggc aatctgctga tgtgacttgg ccagcgcggc actgagcgca tcggagccga gtagcagccg cagtggcgga tcgtcggcca taaccacctc gatgatcgcg tgcgcggcgc gggcgggatc gcccggagcg ttgcggttga aaccagcgcg gcggcggttc atcgcaccga cggtgctttc gtagtccggc gagacggtcg cgacggccat cgacgagccg gcccattccg tgcgcaaggc ccccggttcg gcgatcgtca ccttgatacc gagcggagct acctccgcgg cgagtgcctc cgagaaccct gcgacggcga acttggacgc ctggtacgcg gcgatgcccg gggatccacc aacgcggccg ccaaccgagg agatctgcac gatgtggccg gcccgttgcc ggtgcagcac ggagagcgcg gctttcgtga cgttcacgac accgaagaag ttagtttcca cctgcgcgcg gaaatcgtcg tcggaaacct cctccacggg tgcggaattg gcgtaaccgg cgttgttcac cacgacgtcg atccggccga acgccgtgac ggcggccgcg atggcacgcc gcgcggcagc tcgatcggtg acgtcgagct cacaagcccg gatccgggga ccgtaccggt cgacgagttc atcgagctgt gcggggcgac gggcggtcgc gacgacctgg tcaccacggt caagcaccgc ggtcgtcagg gcgcggccga gcccgcgcga gctgccggtg atgaaccacg tcttcggcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc catggtat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPKTWFITG SSRGLGRALT TAVLDHGDHV VATARRPAQL DELVDRYGPR IRACELDVTD RAAARRAIAA AVTAFGRIDV VVNNAGYANS APVEEVSDDD FRAQVETNFF GVVNVTKAAL PVLHRQRAGH IVQISSVGGR VGGSPGIAAY QASKFAVAGF SEALAAEVAP LGIKVTIAEP GALRTEWAGS SMAVATVSPD YESTVGAMNR RRAGFNRNAP GDPARAAHAI IEVVIADDPP LRLLLGSDAL SAALAKSHQQ IAEANRWAEL SKSIDYPPVA TPHRAARSR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgaagacg tggttcatca ccggcagctc gcgcgggctc ggccgcgccc tgacgaccgc ggtgctcgac catggcgacc atgtcgtcgc gaccgcccgt cgccccgcac agctcgatga actcgtcgac cggtacggcc cccggatccg ggcctgtgag ctcgacgtca ccgatcgagc tgccgcgcgg cgtgccatcg cggccgccgt cacggcgttc ggccggatcg acgtcgtggt gaacaacgcc ggttacgcca attccgcacc cgtggaggag gtttccgacg acgatttccg cgcgcaggtg gaaaccaact tcttcggtgt cgtgaacgtc acgaaagccg cgctccccgt gctgcaccgg caacgggccg gccacatcgt gcagatctcc tcggttggcg gccgcgttgg tggatccccg ggcatcgccg cgtaccaggc gtccaagttc gccgtcgcag ggttctcgga ggcactcgcc gcggaggtag ctccgctcgg tatcaaggtg acgatcgccg aaccgggggc cttgcgcacg gaatgggccg gctcgtcgat ggccgtcgcg accgtctcgc cggactacga aagcaccgtc ggtgcgatga accgccgccg cgccggtttc aaccgcaacg ctccgggcga tcccgcccgc gccgcacacg cgatcatcga ggtggttatt gccgacgatc cgccactgcg gctgctactc ggctccgatg cgctcagtgc cgcgctggcc aagtcacatc agcagattgc cgaagcgaac cggtgggcgg agttgagtaa atccatcgac tatccgcccg tcgccacccc acaccgagct gcgcgcagtc gaaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for MyavA.01204.c.A1.PS00831

PURIFICATION DATe:	10/25/2010
CONCENTRATION:	34.28mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCGAAGACG TGGTTCATCA CCGGCAGCTC GCGCGGGCTC GGCCGCGCCC TGACGACCGC GGTGCTCGAC CATGGCGACC ATGTCGTCGC GACCGCCCGT CGCCCCGCAC AGCTCGATGA ACTCGTCGAC CGGTACGGCC CCCGGATCCG GGCCTGTGAG CTCGACGTCA CCGATCGAGC TGCCGCGCGG CGTGCCATCG CGGCCGCCGT CACGGCGTTC GGCCGGATCG ACGTCGTGGT GAACAACGCC GGTTACGCCA ATTCCGCACC CGTGGAGGAG GTTTCCGACG ACGATTTCCG CGCGCAGGTG GAAACCAACT TCTTCGGTGT CGTGAACGTC ACGAAAGCCG CGCTCCCCGT GCTGCACCGG CAACGGGCCG GCCACATCGT GCAGATCTCC TCGGTTGGCG GCCGCGTTGG TGGATCCCCG GGCATCGCCG CGTACCAGGC GTCCAAGTTC GCCGTCGCAG GGTTCTCGGA GGCACTCGCC GCGGAGGTAG CTCCGCTCGG TATCAAGGTG ACGATCGCCG AACCGGGGGC CTTGCGCACG GAATGGGCCG GCTCGTCGAT GGCCGTCGCG ACCGTCTCGC CGGACTACGA AAGCACCGTC GGTGCGATGA ACCGCCGCCG CGCCGGTTTC AACCGCAACG CTCCGGGCGA TCCCGCCCGC GCCGCACACG CGATCATCGA GGTGGTTATT GCCGACGATC CGCCACTGCG GCTGCTACTC GGCTCCGATG CGCTCAGTGC CGCGCTGGCC AAGTCACATC AGCAGATTGC CGAAGCGAAC CGGTGGGCGG AGTTGAGTAA ATCCATCGAC TATCCGCCCG TCGCCACCCC ACACCGAGCT GCGCGCAGTC GAAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA