SSGCID: Target Page

MyavA.01189.b

Oxidoreductase, zinc-binding dehydrogenase family protein

CENTER ID:	MyavA.01189.b
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01189.b.A1.GE29074	Full length( MyavA.01189.b )		1	323

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01189.b.A1.PW28979	Full length( MyavA.01189.b )		1	323

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.3944
RefSeq:	YP_883174.1
UniProt:	A0A0H3A4B5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRAARVTRLD GPDAIEVTDV DEPTGDGVVV DVHAAGVAFP DALLTRGLYQ YRPELPFVLG AEIAGVVRSA PEGAHVRSGD RVVGLTMLSG GMAEVAVLAP DRVFKLPDNV SFEAGAGLLF NDLTVYFALT VRGRLQRGET VLVHGAAGGI GTSALRLAPA LGAARTIAVV STTDKADIAT AAGATDVVLA DGFKDAVKEL THGRGVDIVV DPVGGDRFTD SLRSLAPGGR LLVVGFTGGE IPTVKVNRLL LNNIDVVGVG WGAWTGTHPG ALAEQWAGLE RLLTSGELAP PEPVVYPLDE AAAAVASLEN RTAKGKVVLR VRG

NT Sequence

ATGCGTGCTG CACGAGTGAC TCGACTGGAC GGTCCGGATG CGATCGAGGT GACGGACGTC GACGAACCGA CCGGCGACGG CGTCGTCGTC GACGTGCACG CGGCCGGGGT GGCCTTCCCG GACGCCCTGC TCACCCGCGG CCTCTACCAG TACCGGCCGG AGTTGCCGTT CGTGCTCGGC GCCGAGATCG CCGGCGTGGT GCGGTCCGCG CCCGAGGGCG CGCACGTGCG TTCCGGCGAC CGGGTCGTCG GCCTCACCAT GCTGTCCGGC GGCATGGCCG AGGTTGCGGT GCTGGCCCCC GACCGGGTGT TCAAGCTGCC CGACAACGTC AGCTTCGAGG CCGGCGCCGG CCTGCTGTTC AACGACCTGA CGGTGTACTT CGCGCTGACC GTGCGGGGCC GGCTGCAGCG CGGCGAGACG GTGCTGGTGC ACGGCGCGGC CGGCGGTATC GGCACGTCGG CGCTGCGACT GGCGCCGGCG CTGGGTGCCG CCCGCACCAT CGCCGTGGTC AGCACGACGG ACAAGGCCGA CATCGCCACG GCGGCCGGCG CGACGGACGT CGTGCTGGCC GACGGCTTCA AGGACGCCGT GAAGGAGCTG ACCCACGGCC GCGGCGTCGA CATCGTCGTC GACCCGGTCG GCGGCGACCG GTTCACCGAT TCGCTGCGCT CGCTGGCCCC CGGCGGGCGG CTGCTGGTGG TCGGCTTCAC CGGCGGCGAG ATCCCCACGG TCAAGGTAAA CCGGTTGCTG CTCAACAACA TCGACGTGGT CGGCGTCGGC TGGGGCGCCT GGACGGGCAC CCATCCCGGC GCGCTGGCCG AGCAGTGGGC CGGGCTCGAA CGACTGCTCA CCTCCGGTGA GCTGGCCCCG CCCGAGCCGG TGGTCTATCC GCTCGACGAG GCCGCGGCGG CCGTGGCCTC GCTGGAGAAC CGCACCGCCA AGGGCAAGGT CGTGCTGCGC GTGCGCGGT

Details for MyavA.01189.b.A1.GE29074

HARVESTED ON:	4/9/2010
SEQUENCED ON:	4/29/2010
EXPECTED MW:	35kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMRAARVT RLDGPDAIEV TDVDEPAGDG VVVDVHAAGV AFPDALLTRG LYQYRPELPF VLGAEIAGVV RSAPEGAHVR SGDRVVGLTM LSGGMAEVAV LAPDRVFKLP DNVSFEAGAG LLFNDLTVYF ALTVRGRLQR GETVLVHGAA GGIGTSALRL APALGAARTI AVVSTTDKAD IATAAGATDV VLADGFKDAV KELTHGRGVD IVVDPVGGDR FTDSLRSLAP GGRLLVVGFT GGEIPTVKVN RLLLNNIDVV GVGWGAWTGT HPGALAEQWA GLERLLTSGE LAPPEPVVYP LDEAAAAVAS LENRTAKGKV VLRVRG

Validated NT Sequence

ttattaaccg cgcacgcgca gcacgacctt gcccttggcg gtgcggttct ccagcgaggc cacggccgcc gcggcctcgt cgagcggata gaccaccggc tcgggcgggg ccagctcacc tgaggtgagc agtcgctcga gcccggccca ctgctcggcc agcgcgccgg gatgggtgcc cgtccaggcg ccccagccga cgccaaccac gtcgatgttg ttgagcagca accggtttac cttgaccgtg gggatctcgc caccggtgaa gccgaccacc agcagccgcc cgccgggggc cagcgagcgc agcgaatcgg tgaaccggtc gccgccgacc gggtcgacga cgatgtcgac gccgcggccg tgggtcagct ccttcacggc gtccttgaag ccgtcggcca gcacgacgtc cgtcgcgccg gccgccgtgg cgatgtcggc cttgtccgtc gtgctgacca cggcgatggt gcgggcggca cccagcgccg gcgccagtcg cagcgccgac gtgccgatac cgccggccgc gccgtgcacc agcaccgtct cgccgcgctg cagccggccc cgcacggtca gcgcgaagta caccgtcagg tcgttgaaca gcaggccggc gccggcctcg aagctgacgt tgtcgggcag cttgaacacc cggtcggggg ccagcaccgc cacctcggcc atgccgccgg acagcatggt gaggccgacg acccggtcgc cggaacgcac gtgcgcgccc tcgggcgcgg accgcaccac gccggcgatc tcggcgccga gcacgaacgg caactccggc cggtactggt agaggccgcg ggtgagcagg gcgtccggga aggccacccc ggccgcgtgc acgtcgacga cgacgccgtc gccggccggt tcgtcgacgt ccgtcacctc gatcgcgtcc ggaccgtcca gtcgagtcac tcgtgcagca cgcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRAARVTRL DGPDAIEVTD VDEPTGDGVV VDVHAAGVAF PDALLTRGLY QYRPELPFVL GAEIAGVVRS APEGAHVRSG DRVVGLTMLS GGMAEVAVLA PDRVFKLPDN VSFEAGAGLL FNDLTVYFAL TVRGRLQRGE TVLVHGAAGG IGTSALRLAP ALGAARTIAV VSTTDKADIA TAAGATDVVL ADGFKDAVKE LTHGRGVDIV VDPVGGDRFT DSLRSLAPGG RLLVVGFTGG EIPTVKVNRL LLNNIDVVGV GWGAWTGTHP GALAEQWAGL ERLLTSGELA PPEPVVYPLD EAAAAVASLE NRTAKGKVVL RVRG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtgctgca cgagtgactc gactggacgg tccggatgcg atcgaggtga cggacgtcga cgaaccgacc ggcgacggcg tcgtcgtcga cgtgcacgcg gccggggtgg ccttcccgga cgccctgctc acccgcggcc tctaccagta ccggccggag ttgccgttcg tgctcggcgc cgagatcgcc ggcgtggtgc ggtccgcgcc cgagggcgcg cacgtgcgtt ccggcgaccg ggtcgtcggc ctcaccatgc tgtccggcgg catggccgag gttgcggtgc tggcccccga ccgggtgttc aagctgcccg acaacgtcag cttcgaggcc ggcgccggcc tgctgttcaa cgacctgacg gtgtacttcg cgctgaccgt gcggggccgg ctgcagcgcg gcgagacggt gctggtgcac ggcgcggccg gcggtatcgg cacgtcggcg ctgcgactgg cgccggcgct gggtgccgcc cgcaccatcg ccgtggtcag cacgacggac aaggccgaca tcgccacggc ggccggcgcg acggacgtcg tgctggccga cggcttcaag gacgccgtga aggagctgac ccacggccgc ggcgtcgaca tcgtcgtcga cccggtcggc ggcgaccggt tcaccgattc gctgcgctcg ctggcccccg gcgggcggct gctggtggtc ggcttcaccg gcggcgagat ccccacggtc aaggtaaacc ggttgctgct caacaacatc gacgtggtcg gcgtcggctg gggcgcctgg acgggcaccc atcccggcgc gctggccgag cagtgggccg ggctcgaacg actgctcacc tccggtgagc tggccccgcc cgagccggtg gtctatccgc tcgacgaggc cgcggcggcc gtggcctcgc tggagaaccg caccgccaag ggcaaggtcg tgctgcgcgt gcgcggtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for MyavA.01189.b.A1.PW28979

PURIFICATION DATe:	10/11/2010
CONCENTRATION:	25.82mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGTGCTGCA CGAGTGACTC GACTGGACGG TCCGGATGCG ATCGAGGTGA CGGACGTCGA CGAACCGACC GGCGACGGCG TCGTCGTCGA CGTGCACGCG GCCGGGGTGG CCTTCCCGGA CGCCCTGCTC ACCCGCGGCC TCTACCAGTA CCGGCCGGAG TTGCCGTTCG TGCTCGGCGC CGAGATCGCC GGCGTGGTGC GGTCCGCGCC CGAGGGCGCG CACGTGCGTT CCGGCGACCG GGTCGTCGGC CTCACCATGC TGTCCGGCGG CATGGCCGAG GTTGCGGTGC TGGCCCCCGA CCGGGTGTTC AAGCTGCCCG ACAACGTCAG CTTCGAGGCC GGCGCCGGCC TGCTGTTCAA CGACCTGACG GTGTACTTCG CGCTGACCGT GCGGGGCCGG CTGCAGCGCG GCGAGACGGT GCTGGTGCAC GGCGCGGCCG GCGGTATCGG CACGTCGGCG CTGCGACTGG CGCCGGCGCT GGGTGCCGCC CGCACCATCG CCGTGGTCAG CACGACGGAC AAGGCCGACA TCGCCACGGC GGCCGGCGCG ACGGACGTCG TGCTGGCCGA CGGCTTCAAG GACGCCGTGA AGGAGCTGAC CCACGGCCGC GGCGTCGACA TCGTCGTCGA CCCGGTCGGC GGCGACCGGT TCACCGATTC GCTGCGCTCG CTGGCCCCCG GCGGGCGGCT GCTGGTGGTC GGCTTCACCG GCGGCGAGAT CCCCACGGTC AAGGTAAACC GGTTGCTGCT CAACAACATC GACGTGGTCG GCGTCGGCTG GGGCGCCTGG ACGGGCACCC ATCCCGGCGC GCTGGCCGAG CAGTGGGCCG GGCTCGAACG ACTGCTCACC TCCGGTGAGC TGGCCCCGCC CGAGCCGGTG GTCTATCCGC TCGACGAGGC CGCGGCGGCC GTGGCCTCGC TGGAGAACCG CACCGCCAAG GGCAAGGTCG TGCTGCGCGT GCGCGGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA