MyavA.01189.a

Alcohol dehydrogenase, zinc-binding

CENTER ID: MyavA.01189.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01189.a.A1.GE29073 Full length( MyavA.01189.a ) 1 322
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.300
RefSeq: YP_879597.1
UniProt: A0A0H2ZSN2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRAVVCRSYG TPEDLVLDEV PDPVPGPGQL LVRVRAAAVN FPDVLFIAGK YQVKIPPPFI PGNEIAGEVI AAGEGARFLP GQRVAGTTFG AFAEQALLDA DQAEPIPDDA DFASAAAFGV TYRTAYHALR STAGVTAGDW VVVLGAAGGV GLAAVDLAVA MQARVLAAAS SPEKLELCRR RGAEATVDYD REDLKTRIRD LTGDRARVVL DPVGGPYSEP ALRGLARGGT FVTLGYASGT IPAIPLNLVL LKDICVRGME IRTFMTDRPD DAVRDLTELS QMFAAGTVRP YIGARFPLAR TAAALRHVAD RKVLGKVVID VA
NT Sequence
ATGCGGGCGG TCGTCTGCCG TTCCTACGGC ACACCGGAGG ACCTGGTGCT CGACGAGGTG CCCGACCCGG TGCCCGGCCC CGGCCAGCTG CTGGTTCGGG TCCGCGCGGC CGCGGTCAAC TTCCCCGACG TACTGTTCAT CGCCGGCAAA TATCAGGTGA AGATTCCGCC GCCGTTCATT CCCGGCAACG AGATCGCCGG CGAGGTCATC GCCGCCGGCG AAGGCGCCCG GTTCCTTCCG GGGCAGCGGG TGGCCGGAAC CACGTTCGGG GCGTTCGCCG AGCAGGCGCT GCTGGACGCC GATCAGGCCG AGCCCATCCC CGACGACGCC GACTTCGCCT CCGCCGCGGC CTTCGGCGTC ACCTACCGCA CCGCCTATCA CGCGCTGCGC TCGACCGCCG GTGTGACGGC GGGAGATTGG GTGGTGGTGC TCGGCGCCGC GGGCGGGGTC GGGCTGGCCG CCGTGGATCT GGCGGTCGCG ATGCAGGCCC GGGTGCTGGC CGCCGCGTCC AGCCCGGAGA AGCTCGAGCT GTGCCGGCGC CGCGGGGCGG AGGCGACGGT GGATTACGAC CGCGAGGACC TCAAGACGCG GATCCGGGAT CTCACCGGCG ACCGCGCCCG CGTGGTCCTG GACCCGGTCG GCGGGCCCTA CTCGGAGCCG GCCCTGCGCG GCCTGGCCCG CGGCGGCACC TTCGTCACCC TGGGCTACGC GTCCGGCACC ATCCCGGCCA TTCCGCTCAA TCTCGTTCTG CTCAAGGACA TCTGCGTGCG CGGCATGGAG ATCCGCACCT TCATGACCGA CCGGCCCGAC GACGCCGTGC GCGACCTGAC GGAGTTGTCG CAGATGTTCG CCGCCGGCAC CGTCCGGCCG TACATCGGGG CGCGGTTTCC GCTCGCGCGG ACCGCCGCGG CGTTGCGGCA CGTCGCCGAC CGCAAGGTGC TGGGCAAGGT GGTGATCGAC GTCGCG
Details for MyavA.01189.a.A1.GE29073
HARVESTED ON: 4/9/2010
SEQUENCED ON: 4/29/2010
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRAVVCRSY GTPEDLVLDE VPDPVPGPGQ LLVRVRAAAV NFPDVLFIAG KYQVKIPPPF IPGNEIAGEV IAAGEGARFL PGQRVAGTTF GAFAEQALLD ADQAEPIPDD ADFASAAAFG VTYRTAYHAL RSTAGVTAGD WVVVLGAAGG VGLAAVDLAV AMQARVLAAA SSPEKLELCR RRGAEATVDY DREDLKTRIR DLTGDRARVV LDPVGGPYSE PALRGLARGG TFVTLGYASG TIPAIPLNLV LLKDICVRGM EIRTFMTDRP DDAVRDLTEL SQMFAAGTVR PYIGARFPLA RTAAALRXVA DRKVLGKVVI DVA
Validated NT Sequence
ttattacgcg acgtcgatca ccaccttgcc cagcaccttg cggtcggcga cntgccgcaa cgccgcggcg gtccgcgcga gcggaaaccg cgccccgatg tacggccgga cggtgccggc ggcgaacatc tgcgacaact ccgtcaggtc gcgcacggcg tcgtcgggcc ggtcggtcat gaaggtgcgg atctccatgc cgcgcacgca gatgtccttg agcagaacga gattgagcgg aatggccggg atggtgccgg acgcgtagcc cagggtgacg aaggtgccgc cgcgggccag gccgcgcagg gccggctccg agtagggccc gccgaccggg tccaggacca cgcgggcgcg gtcgccggtg agatcccgga tccgcgtctt gaggtcctcg cggtcgtaat ccaccgtcgc ctccgccccg cggcgccggc acagctcgag cttctccggg ctggacgcgg cggccagcac ccgggcctgc atcgcgaccg ccagatccac ggcggccagc ccgaccccgc ccgcggcgcc gagcaccacc acccaatctc ccgccgtcac accggcggtc gagcgcagcg cgtgataggc ggtgcggtag gtgacgccga aggccgcggc ggaggcgaag tcggcgtcgt cggggatggg ctcggcctga tcggcgtcca gcagcgcctg ctcggcgaac gccccgaacg tggttccggc cacccgctgc cccggaagga accgggcgcc ttcgccggcg gcgatgacct cgccggcgat ctcgttgccg ggaatgaacg gcggcggaat cttcacctga tatttgccgg cgatgaacag tacgtcgggg aagttgaccg cggccgcgcg gacccgaacc agcagctggc cggggccggg caccgggtcg ggcacctcgt cgagcaccag gtcctccggt gtgccgtagg aacggcagac gaccgcccgc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccatggtat atctccttct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRAVVCRSY GTPEDLVLDE VPDPVPGPGQ LLVRVRAAAV NFPDVLFIAG KYQVKIPPPF IPGNEIAGEV IAAGEGARFL PGQRVAGTTF GAFAEQALLD ADQAEPIPDD ADFASAAAFG VTYRTAYHAL RSTAGVTAGD WVVVLGAAGG VGLAAVDLAV AMQARVLAAA SSPEKLELCR RRGAEATVDY DREDLKTRIR DLTGDRARVV LDPVGGPYSE PALRGLARGG TFVTLGYASG TIPAIPLNLV LLKDICVRGM EIRTFMTDRP DDAVRDLTEL SQMFAAGTVR PYIGARFPLA RTAAALRHVA DRKVLGKVVI DVA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgggcggtc gtctgccgtt cctacggcac accggaggac ctggtgctcg acgaggtgcc cgacccggtg cccggccccg gccagctgct ggttcgggtc cgcgcggccg cggtcaactt ccccgacgta ctgttcatcg ccggcaaata tcaggtgaag attccgccgc cgttcattcc cggcaacgag atcgccggcg aggtcatcgc cgccggcgaa ggcgcccggt tccttccggg gcagcgggtg gccggaacca cgttcggggc gttcgccgag caggcgctgc tggacgccga tcaggccgag cccatccccg acgacgccga cttcgcctcc gccgcggcct tcggcgtcac ctaccgcacc gcctatcacg cgctgcgctc gaccgccggt gtgacggcgg gagattgggt ggtggtgctc ggcgccgcgg gcggggtcgg gctggccgcc gtggatctgg cggtcgcgat gcaggcccgg gtgctggccg ccgcgtccag cccggagaag ctcgagctgt gccggcgccg cggggcggag gcgacggtgg attacgaccg cgaggacctc aagacgcgga tccgggatct caccggcgac cgcgcccgcg tggtcctgga cccggtcggc gggccctact cggagccggc cctgcgcggc ctggcccgcg gcggcacctt cgtcaccctg ggctacgcgt ccggcaccat cccggccatt ccgctcaatc tcgttctgct caaggacatc tgcgtgcgcg gcatggagat ccgcaccttc atgaccgacc ggcccgacga cgccgtgcgc gacctgacgg agttgtcgca gatgttcgcc gccggcaccg tccggccgta catcggggcg cggtttccgc tcgcgcggac cgccgcggcg ttgcggcacg tcgccgaccg caaggtgctg ggcaaggtgg tgatcgacgt cgcgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa