SSGCID: Target Page

MyavA.01147.a

Cysteine synthase

CENTER ID:	MyavA.01147.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01147.a.A1.GE29067	Full length( MyavA.01147.a )		1	310

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01147.a.A1.PS00676	Full length( MyavA.01147.a )		1	310

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.2002
RefSeq:	YP_881269.1
UniProt:	A0A0H2ZYK8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSIAENVTQL IGNTPLVRLN RVTEGAVADV VAKLEFFNPG NSVKDRIGVA MIDAAEQAGL IKPDTIILEP TSGNTGIALA LVAAARGYRC VLTMPETMSV ERRMLLRALG AEIVLTPGAD GMPGAIAKAE ELAKSDDRYF VPQQFENPAN PAIHRSTTAE EVWRDTDGKV DIFVAGVGTG GTITGVAQVI KERKPSAQFI AVEPAASPVL SGGQKGPHPI QGLGAGFVPP VLAMDLVDEV IAVGNEESIA LARRLAAEEG LLVGISSGAA LVAALQVARR PENAGKLVVV VLPDFGERYL STPLFADLAD

NT Sequence

ATGAGCATCG CCGAGAACGT CACGCAGCTG ATCGGAAACA CCCCGCTGGT CCGGTTGAAC CGGGTCACCG AAGGTGCGGT CGCCGACGTC GTCGCCAAGT TGGAATTCTT CAACCCGGGC AACAGCGTGA AGGACCGTAT CGGGGTGGCC ATGATCGATG CGGCCGAGCA GGCGGGCCTG ATCAAACCGG ACACCATCAT TCTCGAGCCG ACGAGCGGCA ACACCGGTAT CGCGCTGGCG CTGGTGGCCG CGGCGCGCGG CTACCGCTGC GTGCTCACCA TGCCGGAGAC GATGAGCGTG GAGCGGCGAA TGTTGTTGCG CGCCTTGGGC GCCGAGATCG TGCTGACGCC GGGCGCCGAC GGCATGCCGG GCGCGATCGC CAAGGCCGAG GAATTGGCCA AGAGCGACGA CCGGTATTTC GTGCCGCAGC AGTTCGAGAA CCCGGCCAAC CCGGCGATTC ACCGCAGCAC CACCGCGGAG GAAGTGTGGC GCGACACCGA CGGCAAGGTC GACATCTTCG TCGCCGGCGT CGGCACCGGC GGCACCATCA CCGGTGTCGC CCAAGTCATC AAGGAACGCA AGCCGTCGGC GCAGTTCATC GCCGTGGAGC CGGCCGCGTC GCCGGTGTTG TCCGGCGGCC AGAAGGGGCC GCATCCGATC CAGGGTCTCG GCGCTGGGTT CGTGCCGCCG GTGCTTGCCA TGGATCTGGT CGACGAGGTC ATCGCCGTCG GCAACGAGGA GTCGATCGCG CTGGCCCGCC GGCTGGCCGC CGAGGAGGGG CTGCTGGTCG GCATCTCCTC GGGGGCGGCG CTGGTGGCCG CCCTGCAGGT GGCCCGCCGG CCCGAGAACG CCGGCAAGCT GGTGGTCGTC GTGCTCCCCG ACTTCGGTGA GCGGTACCTG AGCACGCCGC TGTTCGCCGA CCTGGCCGAT

Details for MyavA.01147.a.A1.GE29067

HARVESTED ON:	4/9/2010
SEQUENCED ON:	4/29/2010
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

ttattaatcg gccaggtcgg cgaacagcgg cgtgctcagg taccgctcac cgaagtcggg gagcacgacg accaccagct tgccggcgtt ctcgggccgg cgggccacct gcagggcggc caccagcgcc gcccccgagg agatgccgac cagcagcccc tcctcggcgg ccagccggcg ggccagcgcg atcgactcct cgttgccgac ggcgatgacc tcgtcgacca gatccatggc aagcaccggc ggcacgaacc cagcgccgag accctggatc ggatgcggcc ccttctggcc gccggacaac accggcgacg cggccggctc cacggcgatg aactgcgccg acggcttgcg ttccttgatg acttgggcga caccggtgat ggtgccgccg gtgccgacgc cggcgacgaa gatgtcgacc ttgccgtcgg tgtcgcgcca cacttcctcc gcggtggtgc tgcggtgaat cgccgggttg gccgggttct cgaactgctg cggcacgaaa taccggtcgt cgctcttggc caattcctcg gccttggcga tcgcgcccgg catgccgtcg gcgcccggcg tcagcacgat ctcggcgccc aaggcgcgca acaacattcg ccgctccacg ctcatcgtct ccggcatggt gagcacgcag cggtagccgc gcgccgcggc caccagtgcg agcgcgatac cggtgttgcc gctcgtcggc tcgagaatga tggtgtccgg tttgatcagg cccgcctgct cggccgcatc gatcatggcc accccgatac ggtccttcac gctgttgccc gggttgaaga attccaactt ggcgacgacg tcggcgaccg caccttcggt gacccggttc aaccggacca gcggggtgtt tccgatcagc tgcgtgacgt tctcggcgat gctcatcgaa ccaggaccct gggtctgagc ttccagggta c

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSIAENVTQ LIGNTPLVRL NRVTEGAVAD VVAKLEFFNP GNSVKDRIGV AMIDAAEQAG LIKPDTIILE PTSGNTGIAL ALVAAARGYR CVLTMPETMS VERRMLLRAL GAEIVLTPGA DGMPGAIAKA EELAKSDDRY FVPQQFENPA NPAIHRSTTA EEVWRDTDGK VDIFVAGVGT GGTITGVAQV IKERKPSAQF IAVEPAASPV LSGGQKGPHP IQGLGAGFVP PVLAMDLVDE VIAVGNEESI ALARRLAAEE GLLVGISSGA ALVAALQVAR RPENAGKLVV VVLPDFGERY LSTPLFADLA D

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcatcgcc gagaacgtca cgcagctgat cggaaacacc ccgctggtcc ggttgaaccg ggtcaccgaa ggtgcggtcg ccgacgtcgt cgccaagttg gaattcttca acccgggcaa cagcgtgaag gaccgtatcg gggtggccat gatcgatgcg gccgagcagg cgggcctgat caaaccggac accatcattc tcgagccgac gagcggcaac accggtatcg cgctggcgct ggtggccgcg gcgcgcggct accgctgcgt gctcaccatg ccggagacga tgagcgtgga gcggcgaatg ttgttgcgcg ccttgggcgc cgagatcgtg ctgacgccgg gcgccgacgg catgccgggc gcgatcgcca aggccgagga attggccaag agcgacgacc ggtatttcgt gccgcagcag ttcgagaacc cggccaaccc ggcgattcac cgcagcacca ccgcggagga agtgtggcgc gacaccgacg gcaaggtcga catcttcgtc gccggcgtcg gcaccggcgg caccatcacc ggtgtcgccc aagtcatcaa ggaacgcaag ccgtcggcgc agttcatcgc cgtggagccg gccgcgtcgc cggtgttgtc cggcggccag aaggggccgc atccgatcca gggtctcggc gctgggttcg tgccgccggt gcttgccatg gatctggtcg acgaggtcat cgccgtcggc aacgaggagt cgatcgcgct ggcccgccgg ctggccgccg aggaggggct gctggtcggc atctcctcgg gggcggcgct ggtggccgcc ctgcaggtgg cccgccggcc cgagaacgcc ggcaagctgg tggtcgtcgt gctccccgac ttcggtgagc ggtacctgag cacgccgctg ttcgccgacc tggccgataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for MyavA.01147.a.A1.PS00676

PURIFICATION DATe:	7/19/2010
CONCENTRATION:	31.64mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCATCGCC GAGAACGTCA CGCAGCTGAT CGGAAACACC CCGCTGGTCC GGTTGAACCG GGTCACCGAA GGTGCGGTCG CCGACGTCGT CGCCAAGTTG GAATTCTTCA ACCCGGGCAA CAGCGTGAAG GACCGTATCG GGGTGGCCAT GATCGATGCG GCCGAGCAGG CGGGCCTGAT CAAACCGGAC ACCATCATTC TCGAGCCGAC GAGCGGCAAC ACCGGTATCG CGCTGGCGCT GGTGGCCGCG GCGCGCGGCT ACCGCTGCGT GCTCACCATG CCGGAGACGA TGAGCGTGGA GCGGCGAATG TTGTTGCGCG CCTTGGGCGC CGAGATCGTG CTGACGCCGG GCGCCGACGG CATGCCGGGC GCGATCGCCA AGGCCGAGGA ATTGGCCAAG AGCGACGACC GGTATTTCGT GCCGCAGCAG TTCGAGAACC CGGCCAACCC GGCGATTCAC CGCAGCACCA CCGCGGAGGA AGTGTGGCGC GACACCGACG GCAAGGTCGA CATCTTCGTC GCCGGCGTCG GCACCGGCGG CACCATCACC GGTGTCGCCC AAGTCATCAA GGAACGCAAG CCGTCGGCGC AGTTCATCGC CGTGGAGCCG GCCGCGTCGC CGGTGTTGTC CGGCGGCCAG AAGGGGCCGC ATCCGATCCA GGGTCTCGGC GCTGGGTTCG TGCCGCCGGT GCTTGCCATG GATCTGGTCG ACGAGGTCAT CGCCGTCGGC AACGAGGAGT CGATCGCGCT GGCCCGCCGG CTGGCCGCCG AGGAGGGGCT GCTGGTCGGC ATCTCCTCGG GGGCGGCGCT GGTGGCCGCC CTGCAGGTGG CCCGCCGGCC CGAGAACGCC GGCAAGCTGG TGGTCGTCGT GCTCCCCGAC TTCGGTGAGC GGTACCTGAG CACGCCGCTG TTCGCCGACC TGGCCGATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA