SSGCID: Target Page

MyavA.01125.a

Glutamine amidotransferase subunit pdxT

CENTER ID:	MyavA.01125.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01125.a.A1.GE29066	Full length( MyavA.01125.a )		1	198

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.3408
RefSeq:	YP_882664.1
UniProt:	A0QIC6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAPRIGVLA LQGDTREHLA ALREAGAESM PVRRRGELEA VDGLVIPGGE STTMSHLLKD LDLLEPLRGL LADGLPAYGA CAGMILLASE ILDAGAGGRE ALPLRAIDMT VRRNAFGRQV DSFEGDIAFA GLDVPVRAVF IRAPWVERAG DGVEVLARAA GHVVAVRQGA RLATAFHPEM TGDRRIHRLF VDLVTGVV

NT Sequence

GTGAGCGCCC CGCGGATCGG GGTGCTGGCG CTGCAGGGCG ACACCCGCGA GCATCTGGCC GCGCTGCGCG AGGCCGGGGC CGAGTCGATG CCGGTGCGCC GGCGCGGCGA GCTCGAGGCG GTCGACGGGC TGGTCATTCC CGGCGGGGAA TCGACCACCA TGAGCCACCT GCTCAAAGAC CTCGACCTGC TGGAGCCGCT GCGGGGGCTG CTGGCCGACG GCCTGCCCGC CTACGGCGCC TGCGCCGGGA TGATCCTGCT GGCCAGCGAG ATCCTGGACG CCGGCGCCGG CGGGCGGGAG GCGCTGCCGC TGCGGGCGAT CGATATGACG GTGCGCCGCA ATGCTTTTGG ACGCCAGGTC GACTCGTTCG AGGGTGACAT CGCGTTCGCG GGACTGGACG TGCCGGTGCG CGCGGTGTTC ATCCGCGCGC CCTGGGTGGA GCGCGCCGGC GACGGCGTCG AGGTGCTGGC CCGCGCCGCC GGTCACGTCG TCGCGGTGCG CCAGGGCGCC AGGCTGGCCA CGGCGTTTCA CCCCGAGATG ACCGGCGACC GGCGCATCCA CCGGCTGTTC GTCGACCTGG TCACCGGGGT GGTC

Details for MyavA.01125.a.A1.GE29066

HARVESTED ON:	4/9/2010
SEQUENCED ON:	4/29/2010
EXPECTED MW:	23kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVSAPRIGV LALQGDTREH LAALREAGAE SMPVRRRGEL EAVDGLVIPG GESTTMSHLL KDLDLLEPLR GLLADGLPAY GACAGMILLA SEILDAGAGG REALPLRAID MTVRRNAFGR QVDSFEGDIA FAGLDGPVRA VFIRAPWVER AGDGVEVLAR AAGHVVAVRQ GARLATAFHP EMTGDRRIHR LFVDLVTGVV

Validated NT Sequence

ttattagacc accccggtga ccaggtcgac gaacagccgg tggatgcgcc ggtcgccggt catctcgggg tgaaacgccg tggccagcct ggcaccctgg cgcaccgcga cgacgtgacc ggcggcgcgg gccagcacct cgacgccgtc gccggcgcgc tccacccagg gcgcgcggat gaacaccgcg cgcaccggcc cgtccaggcc cgcgaacgcg atgtcaccct cgaacgagtc gacctggcgt ccaaaagcat tgcggcgcac cgtcatatcg atcgcccgca gcggcagcgc ctcccgcccg ccggcgccgg cgtccaggat ctcgctggcc agcaggatca tcccggcgca ggcgccgtag gcgggcagcc cgtcggccag cagcccccgc agcggctcca gcaggtcgag gtctttgagc aggtggctca tggtggtcga ttccccgccg gggatgacca gcccgtcgac cgcctcgagc tcgccacgcc ggcgcaccgg catcgactcg gccccggcct cgcgcagcgc ggccagatgc tcgcgggtgt cgccctgcag cgccagcacc ccgatccgcg gggcgctcac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSAPRIGV LALQGDTREH LAALREAGAE SMPVRRRGEL EAVDGLVIPG GESTTMSHLL KDLDLLEPLR GLLADGLPAY GACAGMILLA SEILDAGAGG REALPLRAID MTVRRNAFGR QVDSFEGDIA FAGLDVPVRA VFIRAPWVER AGDGVEVLAR AAGHVVAVRQ GARLATAFHP EMTGDRRIHR LFVDLVTGVV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcgcc ccgcggatcg gggtgctggc gctgcagggc gacacccgcg agcatctggc cgcgctgcgc gaggccgggg ccgagtcgat gccggtgcgc cggcgcggcg agctcgaggc ggtcgacggg ctggtcattc ccggcgggga atcgaccacc atgagccacc tgctcaaaga cctcgacctg ctggagccgc tgcgggggct gctggccgac ggcctgcccg cctacggcgc ctgcgccggg atgatcctgc tggccagcga gatcctggac gccggcgccg gcgggcggga ggcgctgccg ctgcgggcga tcgatatgac ggtgcgccgc aatgcttttg gacgccaggt cgactcgttc gagggtgaca tcgcgttcgc gggactggac gtgccggtgc gcgcggtgtt catccgcgcg ccctgggtgg agcgcgccgg cgacggcgtc gaggtgctgg cccgcgccgc cggtcacgtc gtcgcggtgc gccagggcgc caggctggcc acggcgtttc accccgagat gaccggcgac cggcgcatcc accggctgtt cgtcgacctg gtcaccgggg tggtcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa