SSGCID: Target Page

MyavA.01124.a

Daunorubicin resistance ATP-binding protein DrrA

CENTER ID:	MyavA.01124.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01124.a.A1.GE29064	Full length( MyavA.01124.a )		1	315

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.3182
RefSeq:	YP_882432.1
UniProt:	A0A0H3A130

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAPAIEVEG LTKKFGGSTL ALAGVDFSVP AGTVCGLLGH NGAGKTTTIN ILSTLIRPTS GRARVAGYDV VRQPAEVRAS IGMAGQFTGM DPMLTGRENL VLFGRLRGLN RRRAKARADE LLEQFNLVDA ADRRLSTYSG GMYRRVDLAS ALVVPPKVLF LDEPTTGLDP RSRRDVWALV SSLRVQGISV LLTTQYLEEA DVLSDSIVVI DHGRVIASGT SEELKRAVGT SYCQVTPANP AELPQVAAAL TGLEGIEIDS DTGSVSVFAP DGVATLVEVF RRVDALGLEL ADISLRKPSL DEAFLHLTER TVARS

NT Sequence

GTGAGCGCAC CGGCGATCGA GGTCGAAGGC CTCACAAAGA AATTCGGGGG GAGCACGCTC GCACTGGCCG GGGTGGACTT CTCGGTTCCG GCCGGGACGG TGTGCGGGCT GCTCGGCCAC AACGGCGCGG GCAAAACCAC TACGATCAAC ATCTTGTCCA CGCTGATCCG CCCGACGTCG GGCCGGGCCC GTGTCGCCGG GTATGACGTC GTTCGCCAGC CGGCCGAGGT ACGCGCCAGC ATCGGGATGG CCGGGCAGTT CACCGGAATG GACCCCATGC TGACGGGCCG GGAGAACCTG GTGTTGTTCG GCAGGCTGCG GGGACTCAAT CGGCGGCGGG CGAAGGCCCG CGCCGACGAA CTGCTCGAAC AGTTCAACCT GGTCGACGCC GCCGACCGGC GGCTGTCGAC CTACTCCGGG GGCATGTACC GCCGCGTCGA TCTGGCCAGC GCACTGGTGG TGCCGCCGAA GGTGCTCTTC CTCGACGAGC CGACCACCGG GCTGGATCCG CGCAGCCGCC GCGACGTGTG GGCCCTGGTG AGTTCGCTGC GGGTGCAGGG CATCTCCGTG CTGCTGACCA CGCAGTACCT GGAAGAGGCC GACGTGCTGA GCGACTCGAT CGTCGTCATC GATCACGGGC GGGTGATCGC CAGCGGCACG TCCGAGGAGC TCAAGCGGGC GGTGGGGACC AGCTACTGCC AGGTGACCCC GGCCAACCCC GCCGAACTTC CCCAGGTAGC GGCCGCGCTA ACAGGGCTCG AAGGGATCGA GATCGACAGC GACACCGGCT CGGTGTCGGT GTTCGCCCCC GACGGGGTGG CCACCCTGGT CGAGGTGTTC CGCCGCGTGG ACGCGCTGGG GCTCGAGCTC GCCGACATCT CGCTGCGCAA GCCGTCGCTG GACGAAGCGT TCCTGCACCT GACCGAACGG ACCGTCGCCC GGTCA

Details for MyavA.01124.a.A1.GE29064

HARVESTED ON:	4/9/2010
SEQUENCED ON:	4/29/2010
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	69

Validated AA Sequence

MAGQFTGMDP MLTGRENLVL FGRLRGLNRR RAKARADELL EQFNLVDAAD RRLSTYSGGM YRRVDLASAL VVPPKVLFLD EPTTGLDPRS RRDVWALVSS LRVQGISVLL TTQYLEEADV LSDSIVVIDH GRVIASGTSE ELKRAVGTSY CQVTPANPAE LPQVATALTG LEGIEIDSDT GSVSVFAPDG VATLVEVFRR VDALGLELAD ISLRKPSLDE AFLHLTERTV ARS

Validated NT Sequence

ttattatgac cgggcgacgg tccgctcggt caggtgcagg aacgcttcgt ccagcgacgg cttgcgcagc gagatgtcgg cgagctcgag ccccagcgcg tctacccggc ggaacacctc gacaagggtg gccaccccgt cgggggcgaa caccgacacc gagccggtgt cgctgtcgat ctcgatccct tcgagccctg ttagcgcggt cgctacctgg ggaagttcgg cggggttggc cggggtcacc tggcagtagc tggtccccac cgcccgcttg agctcctcgg aggtgccgct ggcaatcacc cggccgtgat cgatgacgac gatcgagtcg ctcagcacgt cggcctcttc caggtattgc gtggtcagca gcacggagat gccctgcacc cgcagcgaac tcaccagggc ccacacgtcg cggcggctgc gcggatccag cccggtggtc ggctcgtcga ggaagagcac cttcggcggc accaccagtg cgctggccag atcgacgcgg cggtacatgc ccccggagta ggtcgacagc cgccggtcgg ccgcatcgac caggttgaac tgttcgagca gttcatcggc gcgggccttc gcccgccgcc gatttagtcc ccgcagcctg ccgaacaaca ccaggttctc ccggcccgtc agcatggggt ccattccggt gaactgcccg gccatcccga tgctggcgcg tacctcggcc ggctggcgca cgacgtcata cccgacgaca ctggcccggc ccgacgtcgg gcggatcagc gtggacaaga tgttgatcgt ggtggttttg cccgcgccgt tgtggccgag cagcccgcac accgtcccgg ccggaaccga gaagtccacc ccgggncagt gcgagcgtgc tccccccgaa tttctttgtg aggccttcga cctcgatcgc cggtgcgctc accatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagcca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSAPAIEV EGLTKKFGGS TLALAGVDFS VPAGTVCGLL GHNGAGKTTT INILSTLIRP TSGRARVAGY DVVRQPAEVR ASIGMAGQFT GMDPMLTGRE NLVLFGRLRG LNRRRAKARA DELLEQFNLV DAADRRLSTY SGGMYRRVDL ASALVVPPKV LFLDEPTTGL DPRSRRDVWA LVSSLRVQGI SVLLTTQYLE EADVLSDSIV VIDHGRVIAS GTSEELKRAV GTSYCQVTPA NPAELPQVAA ALTGLEGIEI DSDTGSVSVF APDGVATLVE VFRRVDALGL ELADISLRKP SLDEAFLHLT ERTVARS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcgca ccggcgatcg aggtcgaagg cctcacaaag aaattcgggg ggagcacgct cgcactggcc ggggtggact tctcggttcc ggccgggacg gtgtgcgggc tgctcggcca caacggcgcg ggcaaaacca ctacgatcaa catcttgtcc acgctgatcc gcccgacgtc gggccgggcc cgtgtcgccg ggtatgacgt cgttcgccag ccggccgagg tacgcgccag catcgggatg gccgggcagt tcaccggaat ggaccccatg ctgacgggcc gggagaacct ggtgttgttc ggcaggctgc ggggactcaa tcggcggcgg gcgaaggccc gcgccgacga actgctcgaa cagttcaacc tggtcgacgc cgccgaccgg cggctgtcga cctactccgg gggcatgtac cgccgcgtcg atctggccag cgcactggtg gtgccgccga aggtgctctt cctcgacgag ccgaccaccg ggctggatcc gcgcagccgc cgcgacgtgt gggccctggt gagttcgctg cgggtgcagg gcatctccgt gctgctgacc acgcagtacc tggaagaggc cgacgtgctg agcgactcga tcgtcgtcat cgatcacggg cgggtgatcg ccagcggcac gtccgaggag ctcaagcggg cggtggggac cagctactgc caggtgaccc cggccaaccc cgccgaactt ccccaggtag cggccgcgct aacagggctc gaagggatcg agatcgacag cgacaccggc tcggtgtcgg tgttcgcccc cgacggggtg gccaccctgg tcgaggtgtt ccgccgcgtg gacgcgctgg ggctcgagct cgccgacatc tcgctgcgca agccgtcgct ggacgaagcg ttcctgcacc tgaccgaacg gaccgtcgcc cggtcaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa