SSGCID: Target Page

MyavA.01117.e

Acyl-CoA dehydrogenase, central region

CENTER ID:	MyavA.01117.e
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01117.e.A1.GE34921	Full length( MyavA.01117.e )		1	383

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.2490
RefSeq:	YP_881746.1
UniProt:	A0A0H2ZVR3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVNSFDALSA REPELVKLRH AVRDFLESDR AEFGWQPGVD SWLAGWDEEF SGRLGAAGFT GLTIPQRYGG HGLGHLHRYV VTEELIAAGA PVAAHWTADR QVAPALLTYG NEEQRERLLP KIVAGQLFSS IGMSEHGAGS DLAAVQTKAV RADGGWLLSG SKVWTSGAHH AHQVVVLART SPPDPEHRHA GFSQFLVPCD AEGVRIEPII LMSGAHHFNE VLFEQVFVPD ADVLGEVGNG WHQVTSELSF ERSGPERILS TGPLLFAAIR TLGRGPVPDD RTAADIGDLM ARLISLRQLS LSVARTLTHG GDAANQAALV KDLGTLFEAE SVGLIADLLE AAPPNSELEA MLNTAWLHKP MFTLRGGTNE VLRGVIARGM GLR

NT Sequence

GTGGTGAATT CGTTCGACGC GTTGAGTGCC CGTGAGCCCG AGTTGGTCAA GCTGCGGCAC GCCGTGCGCG ACTTCTTGGA GTCCGACCGT GCTGAATTCG GCTGGCAGCC CGGCGTCGAT TCATGGCTGG CCGGGTGGGA CGAGGAGTTC TCGGGGCGGC TTGGCGCCGC CGGATTCACC GGCCTGACCA TCCCGCAGCG TTACGGCGGC CACGGCCTGG GGCACCTGCA CCGCTACGTG GTGACCGAGG AACTGATCGC GGCGGGCGCG CCCGTGGCCG CCCACTGGAC GGCCGACCGT CAGGTCGCAC CCGCCCTGCT GACCTACGGC AATGAGGAGC AGCGCGAACG TTTGTTGCCC AAGATTGTTG CAGGACAGCT TTTTTCGTCA ATCGGCATGA GCGAACACGG CGCCGGGTCC GACCTTGCTG CTGTGCAAAC CAAGGCTGTC CGCGCCGACG GCGGCTGGCT ACTCTCCGGC AGCAAGGTGT GGACCAGCGG GGCCCACCAT GCGCACCAGG TCGTCGTGCT AGCCCGCACC AGCCCGCCGG ATCCAGAACA CCGCCACGCC GGGTTCAGCC AATTCCTGGT GCCGTGCGAT GCCGAGGGCG TCCGCATCGA GCCGATCATC TTGATGTCGG GGGCTCACCA CTTCAACGAG GTCCTGTTCG AGCAGGTCTT CGTTCCCGAT GCCGACGTGC TCGGTGAAGT CGGCAACGGC TGGCACCAGG TCACGTCCGA GTTGTCGTTC GAACGCAGCG GTCCCGAGCG GATCCTGTCC ACCGGTCCCT TGCTGTTCGC CGCCATCCGC ACACTCGGTC GCGGGCCGGT GCCCGACGAC CGTACCGCCG CCGACATCGG CGACCTGATG GCGCGGTTGA TCTCGCTGCG GCAGCTGTCG TTGTCGGTGG CCCGCACGCT CACCCACGGC GGCGACGCGG CAAATCAGGC GGCGCTGGTG AAGGACCTCG GCACCCTTTT CGAAGCCGAA TCCGTCGGGT TGATCGCCGA CCTGCTCGAG GCGGCACCGC CGAATTCCGA ACTGGAGGCC ATGCTGAACA CCGCTTGGCT GCACAAACCC ATGTTCACGC TGCGCGGTGG CACCAACGAG GTGCTACGCG GCGTCATCGC CCGCGGTATG GGGCTGCGA

Details for MyavA.01117.e.A1.GE34921

HARVESTED ON:	4/17/2012
SEQUENCED ON:	4/17/2012
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	76

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVVNSFDAL SAREPELVKL RHAVRDFLES DRAEFGWQPG VDSWLAGWDE EFSGRLGAAG FTGLTIPQRY GGHGLGHLHR YVVTEELIAA GAPVAAHWTA DRQVAPALLT YGNEEQRERL LPKIVAGQLF SSIGMSEHGA GSDLAAVQTK AVRADGGWLL SGSKVWTSGA HHAHQVVVLA RTSPPDPEHR HAGFSQFLVP CDAEGVRIEP IILMSGAHHF NEVLFEQVFV PDADVLGEVG NGWHXXTSEL SFERSGPERI LSTGPLLFAA IRTLGRGPVP XDRTAAXXGX

Validated NT Sequence

tttntttaac tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtggt gaattcgttc gacgcgttga gtgcccgtga gcccgagttg gtcaagctgc ggcacgccgt gcgcgacttc ttggagtccg accgtgctga attcggctgg cagcccggcg tcgattcatg gctggccggg tgggacgagg agttctcggg gcggcttggc gccgccggat tcaccggcct gaccatcccg cagcgttacg gcggccacgg cctggggcac ctgcaccgct acgtggtgac cgaggaactg atcgcggcgg gcgcgcccgt ggccgcccac tggacggccg accgtcaggt cgcacccgcc ctgctgacct acggcaatga ggagcagcgc gaacgtttgt tgcccaagat tgttgcagga cagctttttt cgtcaatcgg catgagcgaa cacggcgccg ggtccgacct tgctgctgtg caaaccaagg ctgtccgcgc cgacggcggc tggctactct ccggcagcaa ggtgtggacc agcggggccc accatgcgca ccaggtcgtc gtgctagccc gcaccagccc gccggatcca gaacaccgcc acgccgggtt cagccaattc ctggtgccgt gcgatgccga gggcgtccgc atcgagccga tcatcttgat gtcgggggct caccacttca acgaggtcct gttcgagcag gtcttcgttc ccgatgccga cgtgctcggt gaagtcggca acggctggca ccngntcacg tccgagttgt cgttcgaacg cagcggtccc gagcggatcc tgtccaccgg tcccttgctg ttcgccgcca tccgcacact cggtcgcggg ccggtgcccn acgaccgtac cgccgccnan ntcggcnact gatggcncgg ttganctcnc tgnggcagct gtcgtngtng nngccngnnn nnnnnnncnn gnnggcngcn aatcnnnngn nnnggnnnnn ncnncngcnc cnntttnnaa nccnaanccn nnnggntnnn nnnncnaccn nnnncnagnn nnnnnnncna nntaantgna ggncnngnnn aannnnnnnn nnnnnnnnnn nnntnnnncn nnnnnnnnnn nnnnnnnnnn ngnnnnnnnn nnnnngnnnn nnnnnnntnn nnnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggtgaat tcgttcgacg cgttgagtgc ccgtgagccc gagttggtca agctgcggca cgccgtgcgc gacttcttgg agtccgaccg tgctgaattc ggctggcagc ccggcgtcga ttcatggctg gccgggtggg acgaggagtt ctcggggcgg cttggcgccg ccggattcac cggcctgacc atcccgcagc gttacggcgg ccacggcctg gggcacctgc accgctacgt ggtgaccgag gaactgatcg cggcgggcgc gcccgtggcc gcccactgga cggccgaccg tcaggtcgca cccgccctgc tgacctacgg caatgaggag cagcgcgaac gtttgttgcc caagattgtt gcaggacagc ttttttcgtc aatcggcatg agcgaacacg gcgccgggtc cgaccttgct gctgtgcaaa ccaaggctgt ccgcgccgac ggcggctggc tactctccgg cagcaaggtg tggaccagcg gggcccacca tgcgcaccag gtcgtcgtgc tagcccgcac cagcccgccg gatccagaac accgccacgc cgggttcagc caattcctgg tgccgtgcga tgccgagggc gtccgcatcg agccgatcat cttgatgtcg ggggctcacc acttcaacga ggtcctgttc gagcaggtct tcgttcccga tgccgacgtg ctcggtgaag tcggcaacgg ctggcaccag gtcacgtccg agttgtcgtt cgaacgcagc ggtcccgagc ggatcctgtc caccggtccc ttgctgttcg ccgccatccg cacactcggt cgcgggccgg tgcccgacga ccgtaccgcc gccgacatcg gcgacctgat ggcgcggttg atctcgctgc ggcagctgtc gttgtcggtg gcccgcacgc tcacccacgg cggcgacgcg gcaaatcagg cggcgctggt gaaggacctc ggcacccttt tcgaagccga atccgtcggg ttgatcgccg acctgctcga ggcggcaccg ccgaattccg aactggaggc catgctgaac accgcttggc tgcacaaacc catgttcacg ctgcgcggtg gcaccaacga ggtgctacgc ggcgtcatcg cccgcggtat ggggctgcga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa