SSGCID: Target Page

MyavA.01097.i

Short chain dehydrogenase

CENTER ID:	MyavA.01097.i
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01097.i.A1.GE29056	Full length( MyavA.01097.i )		1	276

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01097.i.A1.PW28961	Full length( MyavA.01097.i )		1	276

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.3748
RefSeq:	YP_882996.1
UniProt:	A0A0H3A1P0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MARWLITGCS TGLGREIAGA ALQAGHRVVV TARRADAVRG FAEEFGELAL PVALDVTDRD QIAAAVAAAD AAFGGIDVLV NNAGHGYLSA VEEGEDAEVR KLFDVNYFGA VDMIKAVLPG MRARGCGHIV NISSMTGLVA NPPNAYYSST KFALEAVTEA LAAEVRPLGI KVTAIEPGAF RTDWATRSMK ESGTPIADYT DVAARKDLIK QFADHLPGDP RKVAEAVLMV TGLDEPPLRL LLGRDVLKAM RDKIAAMSAS IDEWEAVTKD VNFPGA

NT Sequence

ATGGCACGCT GGCTGATCAC GGGATGCTCC ACCGGCCTCG GCCGCGAAAT CGCCGGTGCG GCACTGCAAG CCGGCCACCG CGTGGTGGTG ACGGCACGAC GGGCCGACGC CGTTCGGGGT TTTGCCGAGG AATTCGGCGA GCTGGCGCTG CCGGTCGCGC TCGACGTGAC CGACCGCGAC CAGATCGCCG CCGCGGTGGC CGCGGCCGAC GCGGCGTTCG GCGGCATCGA CGTGCTGGTG AACAACGCCG GGCACGGCTA CCTGTCGGCG GTGGAAGAGG GCGAGGACGC CGAGGTGCGA AAGCTGTTCG ACGTCAACTA CTTCGGTGCG GTCGACATGA TCAAGGCGGT GCTTCCGGGC ATGCGCGCCC GCGGCTGCGG GCACATCGTC AACATCTCGT CGATGACCGG CCTGGTGGCC AACCCGCCCA ACGCCTACTA CTCGTCGACC AAGTTCGCGC TGGAGGCCGT CACCGAGGCG CTCGCCGCCG AGGTCCGCCC GTTGGGTATC AAGGTGACCG CGATCGAGCC GGGGGCCTTC CGCACCGATT GGGCCACCCG ATCGATGAAG GAGTCCGGCA CGCCGATCGC CGACTACACC GACGTCGCGG CGCGCAAGGA CCTGATCAAA CAGTTCGCCG ACCACCTGCC CGGCGACCCG CGCAAGGTGG CCGAGGCGGT GCTGATGGTG ACCGGGCTGG ACGAGCCGCC GCTGCGGCTG CTGCTCGGAC GCGACGTGCT CAAGGCCATG CGGGACAAGA TCGCCGCGAT GTCGGCGTCG ATCGACGAGT GGGAGGCCGT CACCAAGGAC GTGAACTTCC CGGGCGCG

Details for MyavA.01097.i.A1.GE29056

HARVESTED ON:	4/9/2010
SEQUENCED ON:	4/29/2010
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMARWLITGC STGFGREIAG AALQAGHRVV VTARRADAVR GFAEEFGELA LPVALDVTDR DQIAAAVAAA DAAFGGIDVL VNNAGHGYLS AVEEGEDAEV RKLFDVNYFG AVDMIKAVLP GMRARGCGHI VNISSMTGLV ANPPNAYYSS TKFALEAVTE ALAAEVRPLG IKVTAIEPGA FRTDWATRSM KESGTPIADY TDVAARKDLI KQFADHLPGD PRKVAEAVLM VTGLDEPPLR LLLGRDVLKA MRDKIAAMSA SIDEWEAVTK DVNFPGA

Validated NT Sequence

ttattacgcg cccgggaagt tcacgtcctt ggtgacggcc tcccactcgt cgatcgacgc cgacatcgcg gcgatcttgt cccgcatggc cttgagcacg tcgcgtccga gcagcagccg cagcggcggc tcgtccagcc cggtcaccat cagcaccgcc tcggccacct tgcgcgggtc gccgggcagg tggtcggcga actgcttgat caggtccttg cgcgccgcga cgtcggtgta gtcggcgatc ggcgtgccgg actccttcat cgatcgggtg gcccaatcgg tgcggaaggc ccccggctcg atcgcggtca ccttgatacc caacgggcgg acctcggcgg cgagcgcctc ggtgacggcc tccagcgcga acttggtcga cgagtagtag gcgttgggcg ggttggccac caggccggtc atcgacgaga tgttgacgat gtgcccgcag ccgcgggcgc gcatgcccgg gagcaccgcc ttgatcatgt cgaccgcacc gaagtagttg acgtcgaaca gctttcgcac ctcggcgtcc tcgccctctt ccaccgccga caggtagccg tgcccggcgt tgttcaccag cacgtcgatg ccgccgaacg ccgcgtcggc cgcggccacc gcggcggcga tctggtcgcg gtcggtcacg tcgagcgcga ccggcagcgc cagctcgccg aattcctcgg caaaaccccg aacggcgtcg gcccgtcgtg ccgtcaccac cacgcggtgg ccggcttgca gtgccgcacc ggcgatttcg cggccgaagc cggtggagca tcccgtgatc agccagcgtg ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccatggt atntctcctt cttaa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMARWLITGC STGLGREIAG AALQAGHRVV VTARRADAVR GFAEEFGELA LPVALDVTDR DQIAAAVAAA DAAFGGIDVL VNNAGHGYLS AVEEGEDAEV RKLFDVNYFG AVDMIKAVLP GMRARGCGHI VNISSMTGLV ANPPNAYYSS TKFALEAVTE ALAAEVRPLG IKVTAIEPGA FRTDWATRSM KESGTPIADY TDVAARKDLI KQFADHLPGD PRKVAEAVLM VTGLDEPPLR LLLGRDVLKA MRDKIAAMSA SIDEWEAVTK DVNFPGA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcacgctgg ctgatcacgg gatgctccac cggcctcggc cgcgaaatcg ccggtgcggc actgcaagcc ggccaccgcg tggtggtgac ggcacgacgg gccgacgccg ttcggggttt tgccgaggaa ttcggcgagc tggcgctgcc ggtcgcgctc gacgtgaccg accgcgacca gatcgccgcc gcggtggccg cggccgacgc ggcgttcggc ggcatcgacg tgctggtgaa caacgccggg cacggctacc tgtcggcggt ggaagagggc gaggacgccg aggtgcgaaa gctgttcgac gtcaactact tcggtgcggt cgacatgatc aaggcggtgc ttccgggcat gcgcgcccgc ggctgcgggc acatcgtcaa catctcgtcg atgaccggcc tggtggccaa cccgcccaac gcctactact cgtcgaccaa gttcgcgctg gaggccgtca ccgaggcgct cgccgccgag gtccgcccgt tgggtatcaa ggtgaccgcg atcgagccgg gggccttccg caccgattgg gccacccgat cgatgaagga gtccggcacg ccgatcgccg actacaccga cgtcgcggcg cgcaaggacc tgatcaaaca gttcgccgac cacctgcccg gcgacccgcg caaggtggcc gaggcggtgc tgatggtgac cgggctggac gagccgccgc tgcggctgct gctcggacgc gacgtgctca aggccatgcg ggacaagatc gccgcgatgt cggcgtcgat cgacgagtgg gaggccgtca ccaaggacgt gaacttcccg ggcgcgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for MyavA.01097.i.A1.PW28961

PURIFICATION DATe:	7/26/2010
CONCENTRATION:	29.68mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	11
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCACGCTGG CTGATCACGG GATGCTCCAC CGGCCTCGGC CGCGAAATCG CCGGTGCGGC ACTGCAAGCC GGCCACCGCG TGGTGGTGAC GGCACGACGG GCCGACGCCG TTCGGGGTTT TGCCGAGGAA TTCGGCGAGC TGGCGCTGCC GGTCGCGCTC GACGTGACCG ACCGCGACCA GATCGCCGCC GCGGTGGCCG CGGCCGACGC GGCGTTCGGC GGCATCGACG TGCTGGTGAA CAACGCCGGG CACGGCTACC TGTCGGCGGT GGAAGAGGGC GAGGACGCCG AGGTGCGAAA GCTGTTCGAC GTCAACTACT TCGGTGCGGT CGACATGATC AAGGCGGTGC TTCCGGGCAT GCGCGCCCGC GGCTGCGGGC ACATCGTCAA CATCTCGTCG ATGACCGGCC TGGTGGCCAA CCCGCCCAAC GCCTACTACT CGTCGACCAA GTTCGCGCTG GAGGCCGTCA CCGAGGCGCT CGCCGCCGAG GTCCGCCCGT TGGGTATCAA GGTGACCGCG ATCGAGCCGG GGGCCTTCCG CACCGATTGG GCCACCCGAT CGATGAAGGA GTCCGGCACG CCGATCGCCG ACTACACCGA CGTCGCGGCG CGCAAGGACC TGATCAAACA GTTCGCCGAC CACCTGCCCG GCGACCCGCG CAAGGTGGCC GAGGCGGTGC TGATGGTGAC CGGGCTGGAC GAGCCGCCGC TGCGGCTGCT GCTCGGACGC GACGTGCTCA AGGCCATGCG GGACAAGATC GCCGCGATGT CGGCGTCGAT CGACGAGTGG GAGGCCGTCA CCAAGGACGT GAACTTCCCG GGCGCGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA