SSGCID: Target Page

MyavA.01097.f

Short chain dehydrogenase

CENTER ID:	MyavA.01097.f
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01097.f.A1.GE34919	Full length( MyavA.01097.f )		1	282

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01097.f.A1.PW35534	Full length( MyavA.01097.f )		1	282

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.2787
RefSeq:	YP_882040.1
UniProt:	A0A0H2ZYZ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MADTASIGLK VRDKVVVITG GARGIGLATA TALHKLGAKV AIGDIDEVRV KESGAALDLD VYGKLDVTDP HSFSDFLDEV ERQLGPIDVL VNNAGIMPLG RVVDESDAVT RRILDINVYG VILGSKLALA RMIPRGRGHV INVASLAGET YLAGAATYCA SKHAVVGFTD AARIEYRRSG VKFSVVKPTF VNTELIAGTS GAKGVRNAEP SDIADAIVKL VAHPRPRVRV TRTAGAIIAS QKFMPRALSE GLNRLLGGEH VFTDAVDVEK RQAYEARARG EQ

NT Sequence

ATGGCAGACA CCGCTTCGAT CGGGCTGAAG GTGCGGGACA AGGTCGTCGT CATCACCGGC GGGGCCCGCG GGATCGGGCT GGCCACCGCG ACCGCGCTGC ACAAGCTGGG CGCCAAGGTC GCCATCGGCG ACATCGACGA GGTGCGGGTG AAGGAGTCCG GCGCCGCGCT GGACCTGGAC GTCTACGGGA AGCTCGACGT CACCGACCCG CACTCGTTCT CCGATTTCCT CGACGAGGTC GAGCGTCAGC TCGGCCCGAT CGACGTGCTG GTCAACAACG CCGGCATCAT GCCGCTCGGC CGGGTCGTCG ACGAGTCCGA CGCCGTCACC CGGCGGATCC TGGACATCAA CGTCTACGGC GTGATCCTGG GCAGCAAGCT GGCGCTGGCG CGGATGATTC CGCGCGGGCG GGGACACGTC ATCAACGTCG CCTCGCTGGC CGGCGAGACC TACCTGGCCG GGGCGGCCAC CTACTGCGCC AGCAAGCACG CCGTCGTCGG CTTCACCGAT GCCGCCCGCA TCGAATACCG CCGATCCGGG GTGAAGTTCT CGGTGGTCAA GCCGACGTTC GTGAACACCG AACTGATCGC GGGCACGTCG GGCGCCAAGG GCGTCAGAAA CGCCGAACCG TCCGACATCG CCGACGCGAT CGTCAAGCTG GTGGCCCACC CGCGGCCACG AGTGCGGGTC ACCAGAACCG CGGGCGCGAT CATCGCCTCG CAGAAGTTCA TGCCGCGCGC GCTGTCGGAG GGCCTGAACC GGCTGCTCGG CGGCGAACAC GTCTTCACCG ACGCCGTCGA CGTGGAAAAA CGGCAGGCGT ACGAGGCGCG GGCCCGCGGC GAACAG

Details for MyavA.01097.f.A1.GE34919

HARVESTED ON:	4/17/2012
SEQUENCED ON:	4/17/2012
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	78
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMADTASIGL KVRGKVVVIT GAARGIGLAT ATALHKLGAK VAIGDIDEVR VKESGAALDL DVYGKLDVTD PHSFSDFLDE VERQLGPIDV LVNNAGIMPL GRVVDESDAV TRRILDINVY GVILGSKLAL ARMIPRGRGH IINVASLAGE TYLAGAATYC ASKHAVVGFT DAARIEYRRS GVKFSVVKPT FVNTELIAGT SGRQGRQKRR TVRHRRRDRQ AGGPPAAAGA GHQNRGRDHR LAEVHAARAV GGPEPAARRR TRLHRRRRRG KTAGVRGAGP R

Validated NT Sequence

cgccgcgggc ccgcgcctcg tacgcctgcc gtttttccac gtcgacggcg tcggtgaaga cgtgttcgcc gccgagcagc cggttcaggc cctccgacag cgcgcgcggc atgaacttct gcgaggcgat gatcgcgccc gcggttctgg tgacccgcac ccgcggccgc gggtgggcca ccagcttgac gatcgcgtcg gcgatgtcgg acggttcggc gtttctgacg cccttggcga cccgacgtgc ccgcgatcag ttcggtgttc acgaacgtcg gcttgaccac cgagaacttc accccggacc ggcggtattc gatgcgggcg gcgtcggtga agccgacgac ggcgtgcttg ctggcgcagt aggtggccgc cccggccagg taggtctcgc cggccagcga ggcgacgttg atgatgtgtc cccgcccgcg cggaatcatc cgcgccagcg ccagcttgct gcccaggatc acgccgtaga cgttgatgtc caggatccgc cgggtgacgg cgtcggactc gtcgacgacc cggccgagcg gcatgatgcc ggcgttgttg accagcacgt cgatcgggcc gagctggcgc tcgacctcgt cgaggaaatc ggagaacgag tgcgggtcgg tgacgtcgag cttcccgtag acgtccaggt ccagcgcggc gccggactcc ttcacccgca cctcgtcgat gtcgccgatg gcgaccttgg cgcccagctt gtgcagcgcg gtcgcggtgg ccagcccgat cccgcgggcc gcgccggtga tgacgacgac cttgccccgc accttcagcc cgatcgaagc ggtgtctgcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMADTASIGL KVRDKVVVIT GGARGIGLAT ATALHKLGAK VAIGDIDEVR VKESGAALDL DVYGKLDVTD PHSFSDFLDE VERQLGPIDV LVNNAGIMPL GRVVDESDAV TRRILDINVY GVILGSKLAL ARMIPRGRGH VINVASLAGE TYLAGAATYC ASKHAVVGFT DAARIEYRRS GVKFSVVKPT FVNTELIAGT SGAKGVRNAE PSDIADAIVK LVAHPRPRVR VTRTAGAIIA SQKFMPRALS EGLNRLLGGE HVFTDAVDVE KRQAYEARAR GEQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagacacc gcttcgatcg ggctgaaggt gcgggacaag gtcgtcgtca tcaccggcgg ggcccgcggg atcgggctgg ccaccgcgac cgcgctgcac aagctgggcg ccaaggtcgc catcggcgac atcgacgagg tgcgggtgaa ggagtccggc gccgcgctgg acctggacgt ctacgggaag ctcgacgtca ccgacccgca ctcgttctcc gatttcctcg acgaggtcga gcgtcagctc ggcccgatcg acgtgctggt caacaacgcc ggcatcatgc cgctcggccg ggtcgtcgac gagtccgacg ccgtcacccg gcggatcctg gacatcaacg tctacggcgt gatcctgggc agcaagctgg cgctggcgcg gatgattccg cgcgggcggg gacacgtcat caacgtcgcc tcgctggccg gcgagaccta cctggccggg gcggccacct actgcgccag caagcacgcc gtcgtcggct tcaccgatgc cgcccgcatc gaataccgcc gatccggggt gaagttctcg gtggtcaagc cgacgttcgt gaacaccgaa ctgatcgcgg gcacgtcggg cgccaagggc gtcagaaacg ccgaaccgtc cgacatcgcc gacgcgatcg tcaagctggt ggcccacccg cggccacgag tgcgggtcac cagaaccgcg ggcgcgatca tcgcctcgca gaagttcatg ccgcgcgcgc tgtcggaggg cctgaaccgg ctgctcggcg gcgaacacgt cttcaccgac gccgtcgacg tggaaaaacg gcaggcgtac gaggcgcggg cccgcggcga acagaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for MyavA.01097.f.A1.PW35534

PURIFICATION DATe:	6/8/2012
CONCENTRATION:	32.15mg/ml
OBSERVED MW:	31kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	78
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCAGACACC GCTTCGATCG GGCTGAAGGT GCGGGACAAG GTCGTCGTCA TCACCGGCGG GGCCCGCGGG ATCGGGCTGG CCACCGCGAC CGCGCTGCAC AAGCTGGGCG CCAAGGTCGC CATCGGCGAC ATCGACGAGG TGCGGGTGAA GGAGTCCGGC GCCGCGCTGG ACCTGGACGT CTACGGGAAG CTCGACGTCA CCGACCCGCA CTCGTTCTCC GATTTCCTCG ACGAGGTCGA GCGTCAGCTC GGCCCGATCG ACGTGCTGGT CAACAACGCC GGCATCATGC CGCTCGGCCG GGTCGTCGAC GAGTCCGACG CCGTCACCCG GCGGATCCTG GACATCAACG TCTACGGCGT GATCCTGGGC AGCAAGCTGG CGCTGGCGCG GATGATTCCG CGCGGGCGGG GACACGTCAT CAACGTCGCC TCGCTGGCCG GCGAGACCTA CCTGGCCGGG GCGGCCACCT ACTGCGCCAG CAAGCACGCC GTCGTCGGCT TCACCGATGC CGCCCGCATC GAATACCGCC GATCCGGGGT GAAGTTCTCG GTGGTCAAGC CGACGTTCGT GAACACCGAA CTGATCGCGG GCACGTCGGG CGCCAAGGGC GTCAGAAACG CCGAACCGTC CGACATCGCC GACGCGATCG TCAAGCTGGT GGCCCACCCG CGGCCACGAG TGCGGGTCAC CAGAACCGCG GGCGCGATCA TCGCCTCGCA GAAGTTCATG CCGCGCGCGC TGTCGGAGGG CCTGAACCGG CTGCTCGGCG GCGAACACGT CTTCACCGAC GCCGTCGACG TGGAAAAACG GCAGGCGTAC GAGGCGCGGG CCCGCGGCGA ACAGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA