SSGCID: Target Page

MyavA.01083.a

Putative thiosulfate sulfurtransferase

CENTER ID:	MyavA.01083.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01083.a.A1.GE29047	Full length( MyavA.01083.a )		1	277

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.735
RefSeq:	YP_880031.1
UniProt:	A0A0H3A1M7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MARSDVLVST DWAESNLDTP GVVFVEVDED TSAYHAGHIP GAIKLDWRSD LQDPVKRDFV DAQQFSKLLS ERGIANDDTV ILYGGNNNWF AAYAYWYFKL YGHEKVKLLD GGRKKWELDG RTLSSDPVSR PATSYTAAAP DNSIRAFRDE VIAAINVKNL VDVRSPDEFS GKILAPAHLP QEQSQRPGHI PGAINVPWSK AANEDGTFKS DEELAALYAA AGLDTGKETI AYCRIGERSS HTWFVLYELL GHRNVKNYDG SWTEYGSLVG APIELGS

NT Sequence

ATGGCACGCT CCGACGTCCT GGTCTCCACC GACTGGGCCG AGAGCAATCT CGACACCCCG GGCGTCGTCT TCGTCGAAGT CGACGAGGAC ACCAGCGCTT ATCACGCCGG GCACATCCCC GGCGCCATCA AGTTGGACTG GCGCTCCGAT CTGCAGGACC CGGTCAAACG CGACTTCGTC GACGCGCAGC AGTTCTCCAA GCTGCTCTCG GAGCGGGGCA TCGCCAACGA CGACACCGTG ATCCTCTACG GCGGCAACAA CAACTGGTTC GCCGCCTACG CGTACTGGTA CTTCAAGCTG TACGGCCACG AGAAGGTCAA GCTGCTCGAC GGCGGCCGCA AGAAGTGGGA GCTGGACGGC CGCACCCTGT CCAGCGACCC CGTCAGCAGG CCGGCGACCT CCTACACCGC GGCCGCACCG GACAACAGCA TCCGGGCGTT CCGGGACGAG GTGATCGCCG CGATCAACGT CAAGAACCTG GTCGACGTGC GCTCCCCGGA CGAGTTCTCC GGCAAGATCC TGGCGCCGGC GCACCTGCCG CAGGAACAAA GCCAGCGGCC CGGCCACATC CCCGGCGCCA TCAATGTGCC GTGGAGCAAG GCCGCCAACG AGGACGGCAC CTTCAAGTCC GACGAGGAAC TGGCCGCGCT GTACGCCGCG GCCGGCCTGG ACACCGGCAA GGAGACGATC GCCTACTGCC GGATCGGCGA GCGGTCGTCG CACACCTGGT TCGTTCTTTA TGAATTGCTC GGGCATCGGA ATGTGAAGAA CTACGACGGC AGTTGGACGG AATACGGCTC CCTGGTGGGT GCCCCGATCG AGTTGGGAAG C

Details for MyavA.01083.a.A1.GE29047

HARVESTED ON:	4/9/2010
SEQUENCED ON:	4/29/2010
EXPECTED MW:	32kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	93
PERCENT COVERAGE:	110

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMARSDVLVS TDWAESNLDT PGVVFVEVDE DTSAYHAGHI PGAIKLDWRS DLQDPVKRDF VDAQQFSKLL SERGIANDDT VILYGGNNNW FAAYAYWYFK LYGHEKVKLL DGGRKKWELD GRTLSSDPVS RPATSYTAAA PDNSIRAFRD EVIAAINVKN LVDVRSPDEF SGKILAPAHL PQEQSQRPGH IPGAINVPWS KAANEDGTFK SDEELAALYA AAGLDTGKET IAYCRIGERS SHTWFVLYEL LGHRXCXXLR RQLDGIRAPX XVPRSSWEAX KQHEQVLXPS FSRXXAAXKX XRKLSXXLXX

Validated NT Sequence

ttgttnnnct ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcacgc tccgacgtcc tggtctccac cgactgggcc gagagcaatc tcgacacccc gggcgtcgtc ttcgtcgaag tcgacgagga caccagcgct tatcacgccg ggcacatccc cggcgccatc aagttggact ggcgctccga tctgcaggac ccggtcaaac gcgacttcgt cgacgcgcag cagttctcca agctgctctc ggagcggggc atcgccaacg acgacaccgt gatcctctac ggcggcaaca acaactggtt cgccgcctac gcgtactggt acttcaagct gtacggccac gagaaggtca agctgctcga cggcggccgc aagaagtggg agctggacgg ccgcaccctg tccagcgacc ccgtcagcag gccggcgacc tcctacaccg cggccgcacc ggacaacagc atccgggcgt tccgggacga ggtgatcgcc gcgatcaacg tcaagaacct ggtcgacgta cgctccccgg acgagttctc cggcaagatc ctcgcgccgg cgcacctgcc gcaggaacaa agccagcggc ccggccacat ccccggcgcc atcaacgtgc cgtggagcaa ggccgccaac gaggacggca ccttcaagtc cgacgaggaa ctggccgcgc tgtacgccgc ggccggcctg gacaccggca aggagacgat cgcctactgc cggatcggcg agcggtcgtc gcacacctgg ttcgttcttt atgaattgct cgggcatcgg naatgtgnnn aactacgacg gcagttggac ggaatacggg ctccnnggng ggtgccccga tcgagttggg aagctnataa acagcacgaa caagttctgc anccaagctt ctcgaggntc cnggctgcta ncaaanncna aaggaagctg agtnngnngc tgcnncnctg annnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcacgctcc gacgtcctgg tctccaccga ctgggccgag agcaatctcg acaccccggg cgtcgtcttc gtcgaagtcg acgaggacac cagcgcttat cacgccgggc acatccccgg cgccatcaag ttggactggc gctccgatct gcaggacccg gtcaaacgcg acttcgtcga cgcgcagcag ttctccaagc tgctctcgga gcggggcatc gccaacgacg acaccgtgat cctctacggc ggcaacaaca actggttcgc cgcctacgcg tactggtact tcaagctgta cggccacgag aaggtcaagc tgctcgacgg cggccgcaag aagtgggagc tggacggccg caccctgtcc agcgaccccg tcagcaggcc ggcgacctcc tacaccgcgg ccgcaccgga caacagcatc cgggcgttcc gggacgaggt gatcgccgcg atcaacgtca agaacctggt cgacgtgcgc tccccggacg agttctccgg caagatcctg gcgccggcgc acctgccgca ggaacaaagc cagcggcccg gccacatccc cggcgccatc aatgtgccgt ggagcaaggc cgccaacgag gacggcacct tcaagtccga cgaggaactg gccgcgctgt acgccgcggc cggcctggac accggcaagg agacgatcgc ctactgccgg atcggcgagc ggtcgtcgca cacctggttc gttctttatg aattgctcgg gcatcggaat gtgaagaact acgacggcag ttggacggaa tacggctccc tggtgggtgc cccgatcgag ttgggaagca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a