SSGCID: Target Page

MyavA.01019.a

Dihydropteroate synthase (DHPS)

CENTER ID:	MyavA.01019.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01019.a.B1.GE43495	full length		1	264

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	A0A0H2ZYK7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNVTDDSFSD GGRYLDPDKA VAHGLALVAE GAGIVDVGGE STRPGATRID PRVETSRVVP VVKALAAEGV TVSIDTMHAD VARAALGSGA RIVNDVSGGR ADPAMAPLLA EAKVPWVLMH WRSVSAERPH AAPQYRDVVA EVRAELLASV DAAVAAGVDS ARLMIDPGLG FAKTGQHNWA LLHALPQLVA TGIPVLLGAS RKRFLGTLLA GPDGAPRPPD GRETATAVIS ALAALHGAWG VRVHDVRATV DALKVLGAWM GDDG

NT Sequence

ttgaacgtca ctgacgattc gttctccgac ggcggccgct acctcgaccc cgacaaggcc gtcgcacacg gactggcgct ggtcgccgag ggcgccggca tcgtcgacgt gggcggcgaa tccacccgcc ccggcgccac ccggatcgac ccgcgcgtcg agacctcccg cgtcgtcccc gtcgtcaaag cgcttgcggc agaaggtgtc acggtgagca tcgacaccat gcacgccgac gtcgcgcggg cggcgctggg cagcggcgcg cggatcgtca acgacgtgtc cggcgggcgc gccgatccgg cgatggcgcc gctgctggcc gaggcgaaag tgccctgggt gctgatgcat tggcgatcgg tgtcggccga gcgcccgcac gcggccccgc agtaccgcga cgtggtcgcc gaggtgcgcg ccgaactgct ggccagcgtc gacgccgcgg tggcagccgg cgtcgattcc gcccggctga tgatcgatcc ggggttggga ttcgccaaga ccggacaaca caattgggcc ctgctgcacg cgttgccgca gttggtcgcc accgggatcc cggtactgct gggcgcctcc cgcaaacggt tcctcggcac gctgctggcc gggcccgacg gcgcgccgcg gccgcccgac gggcgggaga cggcgaccgc ggtgatttcc gcgctggccg cgctgcacgg ggcgtggggg gtgcgggtgc acgacgtgcg ggccacggtc gatgcgctca aggtgctcgg cgcttggatg ggagacgatg gctga

Details for MyavA.01019.a.B1.GE43495

HARVESTED ON:	11/19/2019
SEQUENCED ON:	12/3/2019
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML NVTDDSFSDG GRYLDPDKAV AHGLALVAEG AGIVDVGGES TRPGATRIDP RVETSRVVPV VKALAAEGVT VSIDTMHADV ARAALGSGAR IVNDVSGGRA DPAMAPLLAE AKVPWVLMHW RSVSAERPHA APQYRDVVAE VRAELLASVD AAVAAGVDSA RLMIDPGLGF AKTGQHNWAL LHALPQLVAT GIPVLLGASR KRFLGTLLAG PDGAPRPPDG RETATAVISA LAALHGAWGV RVHDVRATVD ALKVLGAWMG DDG

Validated NT Sequence

atcttactca cttagccatc gtctcccatc caagcgccga gcaccttgag cgcatcgacc gtggcccgca cgtcgtgcac ccgcaccccc cacgccccgt gcagcgcggc cagcgcggaa atcaccgcgg tcgccgtctc ccgcccgtcg ggcggccgcg gcgcgccgtc gggcccggcc agcagcgtgc cgaggaaccg tttgcgggag gcgcccagca gtaccgggat cccggtggcg accaactgcg gcaacgcgtg cagcagggcc caattgtgtt gtccggtctt ggcgaatccc aaccccggat cgatcatcag ccgggcggaa tcgacgccgg ctgccaccgc ggcgtcgacg ctggccagca gttcggcgcg cacctcggcg accacgtcgc ggtactgcgg ggccgcgtgc gggcgctcgg ccgacaccga tcgccaatgc atcagcaccc agggcacttt cgcctcggcc agcagcggcg ccatcgccgg atcggcgcgc ccgccggaca cgtcgttgac gatccgcgcg ccgctgccca gcgccgcccg cgcgacgtcg gcgtgcatgg tgtcgatgct caccgtgaca ccttctgccg caagcgcttt gacgacgggg acgacgcggg aggtctcgac gcgcgggtcg atccgggtgg cgccggggcg ggtggattcg ccgcccacgt cgacgatgcc ggcgccctcg gcgaccagcg ccagtccgtg tgcgacggcc ttgtcggggt cgaggtagcg gccgccgtcg gagaacgaat cgtcagtgac gttcaacata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHMN VTDDSFSDGG RYLDPDKAVA HGLALVAEGA GIVDVGGEST RPGATRIDPR VETSRVVPVV KALAAEGVTV SIDTMHADVA RAALGSGARI VNDVSGGRAD PAMAPLLAEA KVPWVLMHWR SVSAERPHAA PQYRDVVAEV RAELLASVDA AVAAGVDSAR LMIDPGLGFA KTGQHNWALL HALPQLVATG IPVLLGASRK RFLGTLLAGP DGAPRPPDGR ETATAVISAL AALHGAWGVR VHDVRATVDA LKVLGAWMGD DG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgaacg tcactgacga ttcgttctcc gacggcggcc gctacctcga ccccgacaag gccgtcgcac acggactggc gctggtcgcc gagggcgccg gcatcgtcga cgtgggcggc gaatccaccc gccccggcgc cacccggatc gacccgcgcg tcgagacctc ccgcgtcgtc cccgtcgtca aagcgcttgc ggcagaaggt gtcacggtga gcatcgacac catgcacgcc gacgtcgcgc gggcggcgct gggcagcggc gcgcggatcg tcaacgacgt gtccggcggg cgcgccgatc cggcgatggc gccgctgctg gccgaggcga aagtgccctg ggtgctgatg cattggcgat cggtgtcggc cgagcgcccg cacgcggccc cgcagtaccg cgacgtggtc gccgaggtgc gcgccgaact gctggccagc gtcgacgccg cggtggcagc cggcgtcgat tccgcccggc tgatgatcga tccggggttg ggattcgcca agaccggaca acacaattgg gccctgctgc acgcgttgcc gcagttggtc gccaccggga tcccggtact gctgggcgcc tcccgcaaac ggttcctcgg cacgctgctg gccgggcccg acggcgcgcc gcggccgccc gacgggcggg agacggcgac cgcggtgatt tccgcgctgg ccgcgctgca cggggcgtgg ggggtgcggg tgcacgacgt gcgggccacg gtcgatgcgc tcaaggtgct cggcgcttgg atgggagacg atggctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat