MyavA.00951.c

Amidase

CENTER ID: MyavA.00951.c
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00951.c.A1.GE34926 Full length( MyavA.00951.c ) 1 459
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.1982
RefSeq: YP_881251.1
UniProt: A0A0H3A1L1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGRSLHAIHA SQPTLNAFRV VLTESALADA AEADRRRAAG DTAPLLGIPI AVKDDVDIAG VPTAFGTEGS VPPATHDAEV VRRLKAAGAV IVGKTNTCEL GQWPFTSGPG FGHTRNPWSR RHTPGGSSGG SAAAVAAGLV TAAIGSDGAG SIRIPAAWTH LVGIKPQRGR ISTWPLPEAF NGITVNGVLA RTVADAALVL DAASGNVDGD RHKPPPITAS DYVGKAPGPL NIALSTRFPY TGFRPKLHPE ILAATRAVGK QLELLGHTVV PGNPDYGMRL SWDFLARSTA GLRDWEERLG DGVVLDPRTV ANLRLGHLLG QAILRSARRH EAADQRRVGS IFDIVDVVLA PTTAQPPPLA RAFDRLSGFG TDRAMIAACP LTFPWNVLGW PSINVPAGFT SDGLPIGVQL MGPANSEGML ISLAAELEAV SGWASKQPQP WWDQSADTPP APGQPAPTG
NT Sequence
GTGGGTCGCT CGCTGCACGC CATCCATGCC AGCCAGCCCA CGCTGAACGC CTTCCGGGTG GTGCTCACCG AGTCGGCGCT GGCCGACGCC GCCGAGGCCG ATCGGCGCCG CGCGGCCGGG GACACCGCCC CGCTGCTGGG CATTCCGATC GCCGTCAAGG ACGACGTCGA CATTGCCGGC GTGCCCACCG CCTTCGGCAC CGAGGGCTCG GTGCCGCCCG CCACGCACGA TGCCGAGGTG GTTCGCCGCC TCAAGGCCGC CGGCGCGGTG ATCGTCGGCA AAACCAACAC CTGTGAACTC GGGCAATGGC CGTTCACCAG CGGACCGGGC TTCGGGCACA CCCGCAATCC CTGGTCGCGC CGGCACACCC CGGGCGGATC GTCGGGCGGC AGCGCCGCGG CGGTGGCCGC GGGCCTGGTG ACCGCGGCCA TCGGGTCCGA CGGCGCCGGC AGCATCCGCA TCCCGGCGGC GTGGACGCAT CTGGTGGGCA TCAAGCCGCA GCGCGGACGC ATCTCGACCT GGCCGCTGCC GGAGGCGTTC AACGGGATCA CGGTCAACGG CGTGCTGGCC CGCACGGTGG CCGACGCGGC GTTGGTGCTC GACGCGGCGT CCGGCAACGT CGACGGCGAC CGGCACAAAC CGCCCCCGAT CACCGCCTCC GACTATGTGG GCAAGGCGCC CGGTCCGTTG AACATCGCGC TGTCGACCCG GTTCCCCTAC ACGGGGTTTC GGCCGAAGCT GCATCCGGAG ATCCTGGCCG CGACCCGTGC GGTGGGCAAG CAACTCGAGC TGCTGGGCCA CACCGTGGTG CCGGGCAACC CGGACTACGG CATGCGGCTG TCGTGGGATT TTCTCGCCCG CTCCACCGCC GGCCTGCGGG ACTGGGAGGA GCGGTTGGGC GACGGCGTGG TGCTGGACCC CCGCACGGTG GCCAACTTGC GGCTGGGTCA TCTGCTGGGA CAGGCGATCC TGCGCAGCGC CCGCCGCCAC GAGGCCGCCG ACCAGCGCCG GGTGGGCTCG ATCTTCGACA TCGTCGACGT GGTGCTGGCG CCGACCACCG CGCAACCCCC GCCGCTGGCC CGCGCCTTCG ACCGGCTCAG CGGCTTCGGC ACCGACCGCG CCATGATTGC GGCGTGCCCG CTGACCTTCC CGTGGAACGT GCTGGGCTGG CCGTCCATCA ACGTTCCGGC CGGCTTCACC TCCGACGGCC TGCCGATCGG CGTGCAGCTG ATGGGCCCGG CCAACAGCGA GGGCATGCTG ATCTCGCTGG CCGCCGAGTT GGAGGCGGTG TCCGGGTGGG CGAGCAAACA GCCGCAGCCC TGGTGGGACC AGAGCGCCGA CACTCCCCCC GCCCCCGGCC AGCCCGCGCC CACCGGG
Details for MyavA.00951.c.A1.GE34926
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 74
PERCENT COVERAGE: 62
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVGRSLHAI HASQPTLNAF RVVLTESALA DAAEADRRRA AGDTAPLLGI PIAVKDDVXI AGVPTAFGTE GSVPPATHDA XVVRRLKAAG AVIVGKTNTC ELGQWPFTSG PGFXHTRNPW SRRHTPGGSS GGSAAAVAAG LVTAAIGSDG AGSIRIPAAW THLXGHQAAA RTHLDLAAAG GVQRDHGQRR AGPHGGRRGV GARRGVRQRR RRPAQTAPDH RLRLCGQGAR SVEHRAVDPV PLHGVSAEXA SGDPGRDPCG GQXTRAAGXH RGAXQPGLRH
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg gtcgctcgct gcacgccatc catgccagcc agcccacgct gaacgccttc cgggtggtgc tcaccgagtc ggcgctggcc gacgccgccg aggccgatcg gcgccgcgcg gccggggaca ccgccccgct gctgggcatt ccgatcgccg tcaaggacga cgtcnacatt gccggcgtgc ccaccgcctt cggcaccgag ggctcggtgc cgcccgccac gcacgatgcc naggtggttc gccgcctcaa ggccgccggc gcggtgatcg tcggcaaaac caacacctgt gaactcgggc aatggccgtt caccagcgga ccgggnttcn ggcacacccg caatccctgg tcgcgccggc acaccccggg cggatcgtcg ggcggcagcg ccgcggcggt ggccgcgggc ctggtgaccg cggccatcgg gtccgacggc gccggcagca tccgcatccc ggcggcgtgg acgcatctgn nggggcatca agccgcagcg cggacgcatc tcgacctggc cgctgccgga ggcgttcaac gggatcacgg tcaacggcgt gctggcccgc acggtggccg acgcggcgtt ggtgctcgac gcggcgtccg gcaacgtcga cggcgaccgg cacaaaccgc ccccgatcac cgcctccgac tatgtgggca aggcgcccgg tccgttgaac atcgcgctgt cgacccggtt cccctacacg gggtttcngc cgaanctgcn tccggagatc ctggccgcga cccgtgcggt gggcaancaa ctcgagctgc tgggncacac cgtggngccn ggcaacccgg actacgncat g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGRSLHAI HASQPTLNAF RVVLTESALA DAAEADRRRA AGDTAPLLGI PIAVKDDVDI AGVPTAFGTE GSVPPATHDA EVVRRLKAAG AVIVGKTNTC ELGQWPFTSG PGFGHTRNPW SRRHTPGGSS GGSAAAVAAG LVTAAIGSDG AGSIRIPAAW THLVGIKPQR GRISTWPLPE AFNGITVNGV LARTVADAAL VLDAASGNVD GDRHKPPPIT ASDYVGKAPG PLNIALSTRF PYTGFRPKLH PEILAATRAV GKQLELLGHT VVPGNPDYGM RLSWDFLARS TAGLRDWEER LGDGVVLDPR TVANLRLGHL LGQAILRSAR RHEAADQRRV GSIFDIVDVV LAPTTAQPPP LARAFDRLSG FGTDRAMIAA CPLTFPWNVL GWPSINVPAG FTSDGLPIGV QLMGPANSEG MLISLAAELE AVSGWASKQP QPWWDQSADT PPAPGQPAPT G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgggtcgc tcgctgcacg ccatccatgc cagccagccc acgctgaacg ccttccgggt ggtgctcacc gagtcggcgc tggccgacgc cgccgaggcc gatcggcgcc gcgcggccgg ggacaccgcc ccgctgctgg gcattccgat cgccgtcaag gacgacgtcg acattgccgg cgtgcccacc gccttcggca ccgagggctc ggtgccgccc gccacgcacg atgccgaggt ggttcgccgc ctcaaggccg ccggcgcggt gatcgtcggc aaaaccaaca cctgtgaact cgggcaatgg ccgttcacca gcggaccggg cttcgggcac acccgcaatc cctggtcgcg ccggcacacc ccgggcggat cgtcgggcgg cagcgccgcg gcggtggccg cgggcctggt gaccgcggcc atcgggtccg acggcgccgg cagcatccgc atcccggcgg cgtggacgca tctggtgggc atcaagccgc agcgcggacg catctcgacc tggccgctgc cggaggcgtt caacgggatc acggtcaacg gcgtgctggc ccgcacggtg gccgacgcgg cgttggtgct cgacgcggcg tccggcaacg tcgacggcga ccggcacaaa ccgcccccga tcaccgcctc cgactatgtg ggcaaggcgc ccggtccgtt gaacatcgcg ctgtcgaccc ggttccccta cacggggttt cggccgaagc tgcatccgga gatcctggcc gcgacccgtg cggtgggcaa gcaactcgag ctgctgggcc acaccgtggt gccgggcaac ccggactacg gcatgcggct gtcgtgggat tttctcgccc gctccaccgc cggcctgcgg gactgggagg agcggttggg cgacggcgtg gtgctggacc cccgcacggt ggccaacttg cggctgggtc atctgctggg acaggcgatc ctgcgcagcg cccgccgcca cgaggccgcc gaccagcgcc gggtgggctc gatcttcgac atcgtcgacg tggtgctggc gccgaccacc gcgcaacccc cgccgctggc ccgcgccttc gaccggctca gcggcttcgg caccgaccgc gccatgattg cggcgtgccc gctgaccttc ccgtggaacg tgctgggctg gccgtccatc aacgttccgg ccggcttcac ctccgacggc ctgccgatcg gcgtgcagct gatgggcccg gccaacagcg agggcatgct gatctcgctg gccgccgagt tggaggcggt gtccgggtgg gcgagcaaac agccgcagcc ctggtgggac cagagcgccg acactccccc cgcccccggc cagcccgcgc ccaccgggaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa