MyavA.00950.b

Peptidase, M28 family protein

CENTER ID: MyavA.00950.b
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00950.b.A2.GE29038 Mature protein( MyavA.00950.b ) 21 498
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.4657
RefSeq: YP_883865.1
UniProt: A0A0H2ZTX7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVNKFTTFPA LLAVVALTAL ATGCGHRSSG AGQTTGDPAA AEFASGLRNK VTTDAMMTHL TKLQDIANAN NGTRAVGTPG YEASVDYVVK TLRDSGFDVQ TPEFSARVFH AEKPDLTVGG RPVEAHALDF SLGTGPDGVS GPLVAAPPNN PGCAVTDYGS LPVQGAVILL DRGTCPFAQK EDVAAQRGAV AMIIADNVDE QQMGGTLGPS TEVKIPVLSV TKSVGVQLRG QPGPTTIKLT ASAQSFKARN VIAQTKTGSE HDVVMAGAHL DSVPEGPGIN DNGSGVAAVL ETAVRLGSSP PVHNAVRFGF WGAEELGLIG SRNYVESLDL TALKNIALYL NFDMLASPNP GYFTYDGDQS LPMDARGQPV VPEGSAGIER TLVAYLKSAG KTAQDTSFDG RSDYDGFTLA GIPAGGLFSG AEGKMSADQA KLWGGTADQP FDPNYHQKTD TLDHIDRTAL GINGGGVAYA IGLYAQDLGG HNGVPAMADR TRHVLAKS
NT Sequence
ATGGTGAACA AATTCACGAC ATTTCCGGCG CTGCTCGCGG TGGTCGCCCT GACCGCGTTG GCCACCGGCT GCGGGCACCG CTCCTCCGGC GCGGGCCAAA CCACCGGAGA CCCCGCCGCG GCGGAGTTCG CGTCCGGGTT GCGCAACAAG GTCACCACCG ACGCGATGAT GACCCACCTG ACCAAGCTGC AAGACATCGC CAACGCCAAC AACGGCACCC GGGCGGTCGG CACACCCGGC TACGAGGCGA GCGTCGACTA CGTGGTGAAA ACCTTGCGCG ACAGCGGGTT CGACGTGCAG ACCCCGGAGT TCTCGGCCCG GGTGTTCCAC GCCGAAAAGC CCGATCTCAC CGTCGGCGGC AGGCCGGTGG AGGCCCACGC ACTGGACTTC AGCCTGGGCA CCGGGCCCGA CGGGGTGAGC GGCCCGCTGG TCGCCGCCCC GCCCAACAAC CCCGGGTGCG CGGTCACCGA CTACGGCAGC CTGCCGGTGC AGGGCGCGGT GATCCTGCTC GACCGCGGCA CGTGCCCGTT CGCGCAGAAG GAAGATGTCG CCGCGCAGCG CGGCGCGGTG GCGATGATCA TCGCCGACAA CGTCGACGAA CAACAGATGG GCGGCACGCT CGGGCCGTCG ACCGAGGTGA AGATCCCGGT GCTGAGCGTG ACCAAGTCCG TCGGGGTGCA GCTGCGCGGG CAGCCCGGGC CCACCACCAT CAAGCTCACG GCCAGCGCGC AAAGCTTCAA GGCGCGCAAC GTGATCGCGC AGACCAAGAC CGGATCGGAG CACGACGTGG TGATGGCCGG GGCGCACCTG GACAGCGTGC CCGAGGGCCC GGGCATCAAC GACAACGGGT CGGGCGTGGC CGCGGTGCTG GAAACCGCTG TGCGACTGGG CAGTTCGCCT CCGGTGCACA ACGCGGTGCG GTTCGGGTTC TGGGGCGCCG AGGAGCTCGG GCTGATCGGG TCGCGCAACT ACGTCGAGTC GCTGGACTTG ACCGCGCTGA AAAACATTGC GCTGTACCTG AACTTCGACA TGCTCGCCTC GCCGAACCCC GGCTACTTCA CCTATGACGG CGACCAGTCG CTGCCGATGG ACGCCCGTGG TCAACCGGTG GTGCCCGAAG GGTCGGCGGG CATCGAGCGC ACGCTGGTGG CCTACCTGAA GTCGGCCGGC AAGACCGCAC AGGACACCTC GTTCGACGGC CGGTCGGATT ACGACGGGTT CACCCTGGCG GGGATTCCGG CCGGCGGGCT GTTCTCCGGC GCCGAGGGGA AGATGTCCGC CGATCAGGCC AAGCTATGGG GCGGAACCGC CGACCAGCCG TTCGACCCCA ACTACCACCA GAAGACCGAC ACCCTCGACC ACATCGACCG CACCGCGCTG GGGATCAACG GGGGCGGGGT GGCCTACGCG ATCGGTCTCT ACGCCCAAGA CCTCGGCGGC CACAACGGCG TCCCCGCCAT GGCCGACCGC ACCCGGCACG TGCTCGCCAA GTCA
Details for MyavA.00950.b.A2.GE29038
HARVESTED ON: data unavailable
SEQUENCED ON: 4/29/2010
EXPECTED MW: 51kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 35
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMATGCGHRS SGAGQTTGDP AAAEFASGLR NKVTTDAMMT HLTKLQDIAN ANNGTRAVGT PGYEASVDYV VKTLRDSGFD VQTPEFSARV FHAEKPDLTV GGRPVEAHAL DFSLGTGPDG VSGPLVAAPP NNPGCAVTDY GSLPVQGAVI LLDRGTCS
Validated NT Sequence
tggcgagcac gtgccgcggt cgagcaggat caccgcgccc tgcaccggca ggctgccgta gtcggtgacc gcgcacccgg ggttgttggg cggggcggcg accaacggac cgctcacccc gtcgggcccg gtgcccaggc tgaagtccag tgcgtgggcc tccaccggcc tgccgccgac ggtgagatcg ggcttttcgg cgtggaacac ccgggccgag aactccgggg tctgcacgtc gaacccgctg tcgcgcaagg ttttcaccac gtagtcgacg ctcgcctcgt agccgggtgt gccgaccgcc cgggtgccgt tgttggcgtt ggcgatgtct tgcagcttgg tcaggtgggt catcatcgcg tcggtggtga ccttgttgcg caacccggac gcgaactcgg ccgcggcggg gtctccggtg gtttggcccg cgccggagga gcggtggccg cagccggtgg ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMATGCGHRS SGAGQTTGDP AAAEFASGLR NKVTTDAMMT HLTKLQDIAN ANNGTRAVGT PGYEASVDYV VKTLRDSGFD VQTPEFSARV FHAEKPDLTV GGRPVEAHAL DFSLGTGPDG VSGPLVAAPP NNPGCAVTDY GSLPVQGAVI LLDRGTCPFA QKEDVAAQRG AVAMIIADNV DEQQMGGTLG PSTEVKIPVL SVTKSVGVQL RGQPGPTTIK LTASAQSFKA RNVIAQTKTG SEHDVVMAGA HLDSVPEGPG INDNGSGVAA VLETAVRLGS SPPVHNAVRF GFWGAEELGL IGSRNYVESL DLTALKNIAL YLNFDMLASP NPGYFTYDGD QSLPMDARGQ PVVPEGSAGI ERTLVAYLKS AGKTAQDTSF DGRSDYDGFT LAGIPAGGLF SGAEGKMSAD QAKLWGGTAD QPFDPNYHQK TDTLDHIDRT ALGINGGGVA YAIGLYAQDL GGHNGVPAMA DRTRHVLAKS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccaccggc tgcgggcacc gctcctccgg cgcgggccaa accaccggag accccgccgc ggcggagttc gcgtccgggt tgcgcaacaa ggtcaccacc gacgcgatga tgacccacct gaccaagctg caagacatcg ccaacgccaa caacggcacc cgggcggtcg gcacacccgg ctacgaggcg agcgtcgact acgtggtgaa aaccttgcgc gacagcgggt tcgacgtgca gaccccggag ttctcggccc gggtgttcca cgccgaaaag cccgatctca ccgtcggcgg caggccggtg gaggcccacg cactggactt cagcctgggc accgggcccg acggggtgag cggcccgctg gtcgccgccc cgcccaacaa ccccgggtgc gcggtcaccg actacggcag cctgccggtg cagggcgcgg tgatcctgct cgaccgcggc acgtgcccgt tcgcgcagaa ggaagatgtc gccgcgcagc gcggcgcggt ggcgatgatc atcgccgaca acgtcgacga acaacagatg ggcggcacgc tcgggccgtc gaccgaggtg aagatcccgg tgctgagcgt gaccaagtcc gtcggggtgc agctgcgcgg gcagcccggg cccaccacca tcaagctcac ggccagcgcg caaagcttca aggcgcgcaa cgtgatcgcg cagaccaaga ccggatcgga gcacgacgtg gtgatggccg gggcgcacct ggacagcgtg cccgagggcc cgggcatcaa cgacaacggg tcgggcgtgg ccgcggtgct ggaaaccgct gtgcgactgg gcagttcgcc tccggtgcac aacgcggtgc ggttcgggtt ctggggcgcc gaggagctcg ggctgatcgg gtcgcgcaac tacgtcgagt cgctggactt gaccgcgctg aaaaacattg cgctgtacct gaacttcgac atgctcgcct cgccgaaccc cggctacttc acctatgacg gcgaccagtc gctgccgatg gacgcccgtg gtcaaccggt ggtgcccgaa gggtcggcgg gcatcgagcg cacgctggtg gcctacctga agtcggccgg caagaccgca caggacacct cgttcgacgg ccggtcggat tacgacgggt tcaccctggc ggggattccg gccggcgggc tgttctccgg cgccgagggg aagatgtccg ccgatcaggc caagctatgg ggcggaaccg ccgaccagcc gttcgacccc aactaccacc agaagaccga caccctcgac cacatcgacc gcaccgcgct ggggatcaac gggggcgggg tggcctacgc gatcggtctc tacgcccaag acctcggcgg ccacaacggc gtccccgcca tggccgaccg cacccggcac gtgctcgcca agtcaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa