MyavA.00950.a

Peptidase, M28 family protein

CENTER ID: MyavA.00950.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00950.a.A2.GE29037 Mature protein( MyavA.00950.a ) 21 490
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.4656
RefSeq: YP_883864.1
UniProt: A0A0H2ZZC9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVRLGATLL WIAVLLAGCS SAGPGLPTAA PDLGHGLAKK VTADAMMAHL RALQNIANAN GGNRADGTPG FQASVDYVAK ALRDKGFDVQ TPQFDRLYPV SPGKPTLTVA GRGYPVDQAS MLVQTPPGGL TGPAVRPAQP SGCAASDYPG ALPKGAIAVV DDTRCSVVDK QNSAVARGAS ALIVVSAPDG HGAPPTLFTP GYYKQLSVPV AVVNPSGAAA LAGAGGPVRL ILDAVNVKVT SRNVVAQTKT GSAHEVIMVG AHLDGSRAGP GINDNGSGVA AVLETALQLG PLAPVSNAVR FVFWGAGEDG LGGVMDYLFG LDRDQLNDIA LYLNFTMLGS PNAGFFTDDG DQSGPPAPGI SAADVPEGSA GIERTLAGYL NLAGKRAADM PLNTRADYHP FMVAGVPIGG MTTGASQPKT TVQSRLWGGQ AGVPFDPDFQ GPRDTADNIN RDALAVMGSG VGFAVGSYAQ SIGGVNGVPP HDKRHRTRVP
NT Sequence
ATGACCGTCC GGCTGGGCGC GACGCTGCTG TGGATCGCCG TGCTGCTGGC CGGTTGCTCG TCGGCGGGGC CCGGACTGCC GACCGCGGCA CCCGATTTGG GCCACGGCCT GGCCAAGAAG GTGACCGCCG ACGCCATGAT GGCCCACCTG CGCGCCTTGC AGAACATCGC CAACGCCAAC GGCGGCAACC GCGCGGACGG CACCCCGGGC TTTCAGGCCA GCGTCGACTA CGTGGCTAAA GCGCTGCGCG ACAAGGGTTT CGACGTACAG ACGCCGCAGT TCGACCGGCT CTACCCGGTG TCCCCCGGCA AGCCGACGCT GACCGTCGCG GGCCGCGGCT ACCCCGTCGA CCAGGCCTCG ATGTTGGTCC AGACCCCGCC CGGCGGGCTG ACCGGACCCG CCGTCCGCCC GGCCCAGCCG TCGGGTTGCG CGGCCAGCGA CTACCCCGGC GCCCTGCCCA AGGGTGCGAT CGCCGTCGTC GACGACACCC GCTGCTCGGT GGTCGACAAA CAGAACAGCG CGGTGGCCCG GGGCGCGAGC GCGCTGATCG TGGTGAGCGC ACCCGACGGG CACGGCGCCC CGCCCACCTT GTTCACCCCC GGCTACTACA AACAGCTGAG TGTGCCGGTG GCCGTCGTCA ACCCGTCCGG CGCCGCCGCG CTGGCCGGTG CCGGCGGGCC GGTGCGGTTA ATCCTGGATG CAGTCAACGT CAAGGTCACA TCGCGAAACG TGGTGGCGCA GACCAAGACC GGGTCAGCCC ACGAGGTCAT CATGGTCGGT GCGCACCTGG ACGGCTCGCG CGCCGGACCG GGCATCAACG ACAACGGCTC CGGCGTGGCC GCGGTGCTGG AAACCGCGCT GCAGCTGGGT CCGCTGGCGC CGGTGAGCAA CGCGGTGCGG TTCGTGTTCT GGGGCGCCGG GGAAGACGGA CTGGGCGGCG TGATGGATTA CCTGTTCGGC CTGGACCGCG ACCAACTCAA CGACATCGCG CTGTATCTGA ACTTCACCAT GCTCGGCTCG CCCAACGCCG GGTTCTTCAC CGACGACGGC GATCAGTCCG GGCCGCCGGC CCCCGGTATT TCGGCCGCCG ACGTCCCCGA AGGGTCGGCC GGCATCGAGC GCACCCTGGC GGGCTATCTG AACCTGGCCG GCAAGCGTGC GGCGGACATG CCCCTGAACA CCCGGGCCGA CTACCACCCC TTCATGGTGG CCGGTGTGCC GATCGGCGGC ATGACCACCG GAGCCTCGCA ACCGAAAACC ACTGTGCAGT CCCGGTTGTG GGGCGGCCAG GCCGGCGTGC CGTTCGATCC GGACTTCCAA GGTCCCCGCG ACACCGCCGA CAACATCAAC CGGGACGCCC TGGCGGTGAT GGGTTCCGGC GTCGGGTTCG CGGTCGGCAG CTACGCCCAA TCGATCGGCG GGGTCAACGG GGTGCCGCCG CACGACAAGC GGCATCGCAC CCGAGTGCCC
Details for MyavA.00950.a.A2.GE29037
HARVESTED ON: data unavailable
SEQUENCED ON: 4/29/2010
EXPECTED MW: 50kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAGPGLPT AAPDLGHGLA KKVTADAMMA HLRALQNIAN ANGGNRADGT PGFQASVDYV AKALRDKGFD VQTPQFDRLY PVSPGKPTLT VAGRGYPVDQ ASMLVQTPPG GLTGPAVRPA QPSGCAASDY PGALPKGAIA VVDDTRCSVV DKQNSAVARG ASALIVVSAP DGHGAPPTLF TPGYYKQLSV PVAVVNPSGA AALAGAGGPV RLILDAVNVK VTSRNVVAQT KTGSAHEVIM VGAHLDGSRA GPGINDNGSG VAAVLETALQ LGPLAPVSNA VRFVFWGAGE DGLGGVMDYL FGLDRDQLND IALYLNFTML GSPNAGFFTD DGDQSGPPAP GISAADVPEG SAGIERTLAG YLNLAGKRAA DMPLNTRADY HPFMVAGVPI GGMTTGASQP KTTVQSRLWG GQAGVPFDPD FQGPRDTADN INRDALAVMG SGVGFAVGSY AQSIGGVNGV PPHDKRHRTR VP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcggcgggg cccggactgc cgaccgcggc acccgatttg ggccacggcc tggccaagaa ggtgaccgcc gacgccatga tggcccacct gcgcgccttg cagaacatcg ccaacgccaa cggcggcaac cgcgcggacg gcaccccggg ctttcaggcc agcgtcgact acgtggctaa agcgctgcgc gacaagggtt tcgacgtaca gacgccgcag ttcgaccggc tctacccggt gtcccccggc aagccgacgc tgaccgtcgc gggccgcggc taccccgtcg accaggcctc gatgttggtc cagaccccgc ccggcgggct gaccggaccc gccgtccgcc cggcccagcc gtcgggttgc gcggccagcg actaccccgg cgccctgccc aagggtgcga tcgccgtcgt cgacgacacc cgctgctcgg tggtcgacaa acagaacagc gcggtggccc ggggcgcgag cgcgctgatc gtggtgagcg cacccgacgg gcacggcgcc ccgcccacct tgttcacccc cggctactac aaacagctga gtgtgccggt ggccgtcgtc aacccgtccg gcgccgccgc gctggccggt gccggcgggc cggtgcggtt aatcctggat gcagtcaacg tcaaggtcac atcgcgaaac gtggtggcgc agaccaagac cgggtcagcc cacgaggtca tcatggtcgg tgcgcacctg gacggctcgc gcgccggacc gggcatcaac gacaacggct ccggcgtggc cgcggtgctg gaaaccgcgc tgcagctggg tccgctggcg ccggtgagca acgcggtgcg gttcgtgttc tggggcgccg gggaagacgg actgggcggc gtgatggatt acctgttcgg cctggaccgc gaccaactca acgacatcgc gctgtatctg aacttcacca tgctcggctc gcccaacgcc gggttcttca ccgacgacgg cgatcagtcc gggccgccgg cccccggtat ttcggccgcc gacgtccccg aagggtcggc cggcatcgag cgcaccctgg cgggctatct gaacctggcc ggcaagcgtg cggcggacat gcccctgaac acccgggccg actaccaccc cttcatggtg gccggtgtgc cgatcggcgg catgaccacc ggagcctcgc aaccgaaaac cactgtgcag tcccggttgt ggggcggcca ggccggcgtg ccgttcgatc cggacttcca aggtccccgc gacaccgccg acaacatcaa ccgggacgcc ctggcggtga tgggttccgg cgtcgggttc gcggtcggca gctacgccca atcgatcggc ggggtcaacg gggtgccgcc gcacgacaag cggcatcgca cccgagtgcc caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa