SSGCID: Target Page

MyavA.00814.a

Catechol-O-methyltransferase

CENTER ID:	MyavA.00814.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.00814.a.A1.GE29026	Full length( MyavA.00814.a )		1	250

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.3001
RefSeq:	YP_882253.1
UniProt:	A0A0H2ZXR2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDLKRRIPLL RWSVWRMAAG VQNITTTGQI GDGREAAAVE YVLRNARAGD IDDVLATIDR FAYQKSMLIN VGDEKGLILD AAVRRADPDV ALELGTYCGY GALRIARAAP GARVFSVELA EANAANARRI WAHAGVADRV TCVVGTIGDG GRTLDVLANE HGFAAGTLDF VFLDHDKDAY LADLKSIMDR GWLHPGSIVV ADNIRWPGSP KYREYMHVQQ GKRFDTVEHK THAEYQTMLS DLMLESEYLG

NT Sequence

ATGGACCTCA AGCGACGGAT ACCCCTGCTG CGGTGGTCGG TCTGGCGCAT GGCGGCCGGG GTGCAGAACA TCACCACCAC CGGCCAGATC GGTGACGGAC GGGAAGCGGC CGCCGTCGAG TACGTGCTGC GCAACGCGCG GGCCGGCGAC ATCGACGACG TGCTGGCCAC CATCGACAGG TTCGCCTACC AGAAATCGAT GCTGATCAAT GTCGGCGACG AGAAGGGGCT GATCCTCGAC GCCGCGGTGC GCCGCGCCGA CCCGGACGTG GCGCTGGAGC TGGGCACCTA CTGCGGCTAC GGCGCGTTGC GGATCGCCCG GGCCGCCCCG GGCGCCCGGG TGTTCTCGGT CGAACTGGCC GAGGCCAACG CCGCCAACGC CCGCCGGATC TGGGCGCACG CCGGCGTCGC CGACCGGGTG ACCTGCGTGG TGGGCACCAT CGGCGACGGC GGGCGCACCC TGGACGTCCT GGCCAACGAG CACGGATTCG CTGCCGGCAC ACTGGATTTC GTGTTTCTCG ATCACGACAA GGACGCCTAC CTGGCCGACC TGAAGAGCAT CATGGACCGG GGCTGGCTAC ATCCGGGCTC GATCGTGGTC GCCGACAACA TCCGCTGGCC GGGCTCGCCG AAGTACCGCG AGTACATGCA TGTCCAGCAG GGCAAGCGGT TCGACACCGT CGAGCACAAG ACGCATGCCG AGTACCAGAC CATGCTCTCC GACCTGATGC TGGAGTCCGA ATACCTGGGC

Details for MyavA.00814.a.A1.GE29026

HARVESTED ON:	4/9/2010
SEQUENCED ON:	4/29/2010
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDLKRRIPL LRWSVWRMAA GVQNITTTGQ IGDGREAAAV EYVLRNARAG DIDDVLATID RFAYQKSMLI NVGDEKGLIL DAAVRRADPD VALELGTYCG YGALRIARAA PGARVFSVEL AEANAANARR IWAHAGVADR VTCVVGTIGD GGRTLDVLAN EHGFAAGTLD FVFLDHDKDA YLADLKSIMD RGWLHPGSIV VADNIRWPGS PKYREYMHVQ QGKRFDTVEH KTHAEYQTML SDLMLESE

Validated NT Sequence

attcggactc cagcatcagg tcggacagca tggtctggta ctcggcatgc gtcttgtgct cgacggtgtc gaaccgcttg ccctgctgga catgcatgta ctcgcggtac ttcggcgagc ccggccagcg gatgttgtcg gcgaccacga tcgagcccgg atgcagccag ccccggtcca tgatgctctt caggtcggcc aggtaggcgt ccttgtcgtg atcgagaaac acgaaatcca atgtgccggc agcgaatccg tgctcattgg ccaggacgtc cagggtgcgt ccgccgtcgc cgatggtgcc caccacgcag gtcacccggt cggcgacgcc ggcgtgcgcc cagatccggc gggcgttggc ggcgttggcc tcggccagtt cgaccgagaa cacccgggcg cccggggcgg cccgggcgat ccgcaacgcg ccgtagccgc agtaggtgcc cagctccagc gccacgtccg ggtcggcgcg gcgcaccgcg gcgtcgagga tcagcccctt ctcgtcgccg acattgatca gcatcgattt ctggtaggcg aacctgtcga tggtggccag cacgtcgtcg atgtcgccgg cccgcgcgtt gcgcagcacg tactcgacgg cggccgcttc ccgtccgtca ccgatctggc cggtggtggt gatgttctgc accccggccg ccatgcgcca gaccgaccac cgcagcaggg gtatccgtcg cttgaggtcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccatggtat atctccttct taa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacctcaag cgacggatac ccctgctgcg gtggtcggtc tggcgcatgg cggccggggt gcagaacatc accaccaccg gccagatcgg tgacggacgg gaagcggccg ccgtcgagta cgtgctgcgc aacgcgcggg ccggcgacat cgacgacgtg ctggccacca tcgacaggtt cgcctaccag aaatcgatgc tgatcaatgt cggcgacgag aaggggctga tcctcgacgc cgcggtgcgc cgcgccgacc cggacgtggc gctggagctg ggcacctact gcggctacgg cgcgttgcgg atcgcccggg ccgccccggg cgcccgggtg ttctcggtcg aactggccga ggccaacgcc gccaacgccc gccggatctg ggcgcacgcc ggcgtcgccg accgggtgac ctgcgtggtg ggcaccatcg gcgacggcgg gcgcaccctg gacgtcctgg ccaacgagca cggattcgct gccggcacac tggatttcgt gtttctcgat cacgacaagg acgcctacct ggccgacctg aagagcatca tggaccgggg ctggctacat ccgggctcga tcgtggtcgc cgacaacatc cgctggccgg gctcgccgaa gtaccgcgag tacatgcatg tccagcaggg caagcggttc gacaccgtcg agcacaagac gcatgccgag taccagacca tgctctccga cctgatgctg gagtccgaat acctgggcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa