SSGCID: Target Page

MyavA.00762.a

Short chain dehydrogenase

CENTER ID:	MyavA.00762.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.00762.a.A1.GE29016	Full length( MyavA.00762.a )		1	262

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.583
RefSeq:	YP_879882.1
UniProt:	A0A0H3A422

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLSEAPKEI AGHGLLEGKV VIVTAAAGTG IGSATAKRAL AEGADVVISD HHERRLGETA DQLAALGQGR VESVLCDVTS TAQVDALFAS AHARMGRIDV LVNNAGLGGQ TPVVDMTDDE WDRVLDVTLT SVFRATRAAL RYFRDAPHGG VIVNNASVLG WRAQHSQSHY AAAKAGVMAL TRCSAIEAAE YDVRINAVSP SIARHKFLEK TSSADLLDRL SAGEAFGRAA EPWEVAATIA FLASDYSSYL TGEVISVSSQ HP

NT Sequence

ATGAGCCTGT CCGAAGCTCC GAAAGAGATT GCGGGACACG GGCTTCTGGA AGGCAAGGTG GTGATCGTCA CCGCGGCGGC CGGCACGGGT ATCGGCTCGG CGACGGCCAA ACGAGCGCTC GCCGAGGGCG CCGACGTGGT GATCTCCGAC CACCACGAGC GTCGGCTGGG CGAGACCGCC GACCAGCTCG CGGCGCTCGG CCAGGGCCGG GTGGAAAGCG TGCTGTGCGA CGTGACGTCC ACCGCCCAGG TCGATGCGCT GTTCGCGTCG GCCCACGCGC GGATGGGGCG CATCGACGTG CTGGTCAACA ACGCCGGGCT GGGCGGGCAG ACCCCGGTGG TCGACATGAC CGACGACGAG TGGGACCGGG TGCTCGATGT CACCCTGACC TCGGTTTTCC GCGCCACCCG TGCCGCGCTG CGCTACTTCC GCGACGCGCC GCACGGCGGG GTGATCGTCA ACAACGCCAG CGTGCTGGGC TGGCGGGCGC AGCACTCCCA GTCGCACTAC GCCGCCGCCA AGGCCGGGGT GATGGCGTTG ACCCGGTGCA GCGCAATCGA AGCCGCCGAG TACGACGTGC GGATCAACGC GGTGTCGCCG AGCATCGCCC GGCACAAGTT CCTGGAGAAG ACGTCGTCGG CCGACCTGCT CGACCGGCTC TCGGCCGGTG AGGCGTTCGG TCGCGCCGCC GAGCCCTGGG AGGTCGCGGC CACCATCGCG TTTTTGGCCA GCGACTACTC CAGCTACCTG ACCGGCGAGG TCATCTCGGT GTCCAGCCAG CATCCG

Details for MyavA.00762.a.A1.GE29016

HARVESTED ON:	4/9/2010
SEQUENCED ON:	4/29/2010
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSLSEAPKE IAGHGLLEGK VVIVTAAAGT GIGSATAKRA LAEGADVVIS DHHERRLGET ADQLAALGQG RVESVLCDVT STAQVDALFA SAHARMGRID VLVNNAGLGG QTPVVDMTDD EWDRVLDVTL TSVFRATRAA LRYFRDAPHG GVIVNNASVL GWRAQHSQSH YAAAKAGVMA LTRCSAIEAA EYDVRINAVS PSIARHKFLE KTSSADLLDR LSAGEAFGRA AEPWEVAATI AFLASDYSSY LTGEVISVS

Validated NT Sequence

ctggacaccg agatgacctc gccggtcagg tagctggagt agtcgctggc caaaaacgcg atggtggccg cgacctccca gggctcggcg gcgcgaccga acgcctcacc ggccgagagc cggtcgagca ggtcggccga cgacgtcttc tccaggaact tgtgccgggc gatgctcggc gacaccgcgt tgatccgcac gtcgtactcg gcggcttcga ttgcgctgca ccgggtcaac gccatcaccc cggccttggc ggcggcgtag tgcgactggg agtgctgcgc ccgccagccc agcacgctgg cgttgttgac gatcaccccg ccgtgcggcg cgtcgcggaa gtagcgcagc gcggcacggg tggcgcggaa aaccgaggtc agggtgacat cgagcacccg gtcccactcg tcgtcggtca tgtcgaccac cggggtctgc ccgcccagcc cggcgttgtt gaccagcacg tcgatgcgcc ccatccgcgc gtgggccgac gcgaacagcg catcgacctg ggcggtggac gtcacgtcgc acagcacgct ttccacccgg ccctggccga gcgccgcgag ctggtcggcg gtctcgccca gccgacgctc gtggtggtcg gagatcacca cgtcggcgcc ctcggcgagc gctcgtttgg ccgtcgccga gccgataccc gtgccggccg ccgcggtgac gatcaccacc ttgccttcca gaagcccgtg tcccgcaatc tctttcggag cttcggacag gctcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcctgtcc gaagctccga aagagattgc gggacacggg cttctggaag gcaaggtggt gatcgtcacc gcggcggccg gcacgggtat cggctcggcg acggccaaac gagcgctcgc cgagggcgcc gacgtggtga tctccgacca ccacgagcgt cggctgggcg agaccgccga ccagctcgcg gcgctcggcc agggccgggt ggaaagcgtg ctgtgcgacg tgacgtccac cgcccaggtc gatgcgctgt tcgcgtcggc ccacgcgcgg atggggcgca tcgacgtgct ggtcaacaac gccgggctgg gcgggcagac cccggtggtc gacatgaccg acgacgagtg ggaccgggtg ctcgatgtca ccctgacctc ggttttccgc gccacccgtg ccgcgctgcg ctacttccgc gacgcgccgc acggcggggt gatcgtcaac aacgccagcg tgctgggctg gcgggcgcag cactcccagt cgcactacgc cgccgccaag gccggggtga tggcgttgac ccggtgcagc gcaatcgaag ccgccgagta cgacgtgcgg atcaacgcgg tgtcgccgag catcgcccgg cacaagttcc tggagaagac gtcgtcggcc gacctgctcg accggctctc ggccggtgag gcgttcggtc gcgccgccga gccctgggag gtcgcggcca ccatcgcgtt tttggccagc gactactcca gctacctgac cggcgaggtc atctcggtgt ccagccagca tccgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa