MyavA.00738.a

RNA polymerase sigma-F factor (Rv3286c ortholog)

CENTER ID: MyavA.00738.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00738.a.B1.GE37404 full length 1 269
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00738.a.B1.PW37468 full length 1 269
MyavA.00738.a.B1.PW37550 full length 1 269

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.2899
RefSeq: YP_882149.1
UniProt: A0A0H3A0X8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNAIAPTPT AARPTTQSDD SYEDVVEMFL ELRRMPAESH EYRRQRERIV ARCLPLADHV ASHFARRGEG LDDLVQVARL GLMNAVNRFD PAKGPSFIGF AVPTMMGEVR RYFRDYSWGM RVPRRLRELH VQISRTTADL AQQLGRAPNA GELSQVLEVP REEIVECLVA GDAYRLDSLD APQGADSSGT PRSVADSVGD IDPQIEHITN REALRVLVAT LPHREREVLR MRFFESMTQS QIAERIGVSQ MQVSRILANT LRCLRDQLE
NT Sequence
atgaccaacg caatcgctcc gacacctaca gctgcccggc ccaccaccca atccgacgat tcgtacgaag acgtcgtcga gatgttcctg gaactgcgcc ggatgcccgc ggaatcgcac gagtaccgcc gtcagcgtga gcgcatcgtg gcccgatgcc tgcccctggc cgatcacgtg gccagccatt tcgcccgccg cggcgagggt ctcgacgact tggtccaggt cgcgcggctg gggctgatga acgccgtcaa ccgcttcgat ccggcgaagg gtcccagctt catcgggttc gccgtgccca ccatgatggg cgaggtccgc cgctatttcc gcgactacag ctgggggatg cgggtgcccc ggcggctgcg cgagttgcac gtgcagatca gcaggacgac cgccgacctc gcccagcagc tcggccgggc gccgaacgcc ggcgagctct cgcaggtgct cgaggttccg cgggaagaga tcgtcgaatg cctggtcgcc ggcgacgcct atcgcctcga ctcgttggat gcgccgcagg gtgcggacag ttccgggacg ccgcgctcgg tggccgactc ggtgggcgac atcgacccgc agatcgagca catcaccaac cgggaagcgc tgcgggtcct ggtcgccacc ctgccgcacc gcgaacgcga ggtgttgcgg atgcggttct tcgaatccat gacgcagagc cagatagccg agcggatcgg cgtctcgcag atgcaggtct cgcgcatcct ggcaaacacc ctgcgctgcc tgcgcgacca actcgagtaa
Details for MyavA.00738.a.B1.GE37404
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 31kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT NAIAPTPTAA RPTTQSDDSY EDVVEMFLEL RRMPAESHEY RRQRERIVAR CLPLADHVAS HFARRGEGLD DLVQVARLGL MNAVNRFDPA KGPSFIGFAV PTMMGEVRRY FRDYSWGMRV PRRLRELHVQ ISRTTADLAQ QLGRAPNAGE LSQVLEVPRE EIVECLVAGD AYRLDSLDAP QGADSSGTPR SVADSVGDID PQIEHITNRE ALRVLVATLP HREREVLRMR FFESMTQSQI AERIGVSQMQ VSRILANTLR CLRDQLE
Validated NT Sequence
atcttactca cttactcgag ttggtcgcgc aggcagcgca gggtgtttgc caggatgcgc gagacctgca tctgcgagac gccgatccgc tcggctatct ggctctgcgt catggattcg aagaaccgca tccgcaacac ctcgcgttcg cggtgcggca gggtggcgac caggacccgc agcgcttccc ggttggtgat gtgctcgatc tgcgggtcga tgtcgcccac cgagtcggcc accgagcgcg gcgtcccgga actgtccgca ccctgcggcg catccaacga gtcgaggcga taggcgtcgc cggcgaccag gcattcgacg atctcttccc gcggaacctc gagcacctgc gagagctcgc cggcgttcgg cgcccggccg agctgctggg cgaggtcggc ggtcgtcctg ctgatctgca cgtgcaactc gcgcagccgc cggggcaccc gcatccccca gctgtagtcg cggaaatagc ggcggacctc gcccatcatg gtgggcacgg cgaacccgat gaagctggga cccttcgccg gatcgaagcg gttgacggcg ttcatcagcc ccagccgcgc gacctggacc aagtcgtcga gaccctcgcc gcggcgggcg aaatggctgg ccacgtgatc ggccaggggc aggcatcggg ccacgatgcg ctcacgctga cggcggtact cgtgcgattc cgcgggcatc cggcgcagtt ccaggaacat ctcgacgacg tcttcgtacg aatcgtcgga ttgggtggtg ggccgggcag ctgtaggtgt cggagcgatt gcgttggtca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMT NAIAPTPTAA RPTTQSDDSY EDVVEMFLEL RRMPAESHEY RRQRERIVAR CLPLADHVAS HFARRGEGLD DLVQVARLGL MNAVNRFDPA KGPSFIGFAV PTMMGEVRRY FRDYSWGMRV PRRLRELHVQ ISRTTADLAQ QLGRAPNAGE LSQVLEVPRE EIVECLVAGD AYRLDSLDAP QGADSSGTPR SVADSVGDID PQIEHITNRE ALRVLVATLP HREREVLRMR FFESMTQSQI AERIGVSQMQ VSRILANTLR CLRDQLE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacca acgcaatcgc tccgacacct acagctgccc ggcccaccac ccaatccgac gattcgtacg aagacgtcgt cgagatgttc ctggaactgc gccggatgcc cgcggaatcg cacgagtacc gccgtcagcg tgagcgcatc gtggcccgat gcctgcccct ggccgatcac gtggccagcc atttcgcccg ccgcggcgag ggtctcgacg acttggtcca ggtcgcgcgg ctggggctga tgaacgccgt caaccgcttc gatccggcga agggtcccag cttcatcggg ttcgccgtgc ccaccatgat gggcgaggtc cgccgctatt tccgcgacta cagctggggg atgcgggtgc cccggcggct gcgcgagttg cacgtgcaga tcagcaggac gaccgccgac ctcgcccagc agctcggccg ggcgccgaac gccggcgagc tctcgcaggt gctcgaggtt ccgcgggaag agatcgtcga atgcctggtc gccggcgacg cctatcgcct cgactcgttg gatgcgccgc agggtgcgga cagttccggg acgccgcgct cggtggccga ctcggtgggc gacatcgacc cgcagatcga gcacatcacc aaccgggaag cgctgcgggt cctggtcgcc accctgccgc accgcgaacg cgaggtgttg cggatgcggt tcttcgaatc catgacgcag agccagatag ccgagcggat cggcgtctcg cagatgcagg tctcgcgcat cctggcaaac accctgcgct gcctgcgcga ccaactcgag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for MyavA.00738.a.B1.PW37468
PURIFICATION DATe: 3/25/2014
CONCENTRATION: 4.41mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT NAIAPTPTAA RPTTQSDDSY EDVVEMFLEL RRMPAESHEY RRQRERIVAR CLPLADHVAS HFARRGEGLD DLVQVARLGL MNAVNRFDPA KGPSFIGFAV PTMMGEVRRY FRDYSWGMRV PRRLRELHVQ ISRTTADLAQ QLGRAPNAGE LSQVLEVPRE EIVECLVAGD AYRLDSLDAP QGADSSGTPR SVADSVGDID PQIEHITNRE ALRVLVATLP HREREVLRMR FFESMTQSQI AERIGVSQMQ VSRILANTLR CLRDQLE
Validated NT Sequence
atcttactca cttactcgag ttggtcgcgc aggcagcgca gggtgtttgc caggatgcgc gagacctgca tctgcgagac gccgatccgc tcggctatct ggctctgcgt catggattcg aagaaccgca tccgcaacac ctcgcgttcg cggtgcggca gggtggcgac caggacccgc agcgcttccc ggttggtgat gtgctcgatc tgcgggtcga tgtcgcccac cgagtcggcc accgagcgcg gcgtcccgga actgtccgca ccctgcggcg catccaacga gtcgaggcga taggcgtcgc cggcgaccag gcattcgacg atctcttccc gcggaacctc gagcacctgc gagagctcgc cggcgttcgg cgcccggccg agctgctggg cgaggtcggc ggtcgtcctg ctgatctgca cgtgcaactc gcgcagccgc cggggcaccc gcatccccca gctgtagtcg cggaaatagc ggcggacctc gcccatcatg gtgggcacgg cgaacccgat gaagctggga cccttcgccg gatcgaagcg gttgacggcg ttcatcagcc ccagccgcgc gacctggacc aagtcgtcga gaccctcgcc gcggcgggcg aaatggctgg ccacgtgatc ggccaggggc aggcatcggg ccacgatgcg ctcacgctga cggcggtact cgtgcgattc cgcgggcatc cggcgcagtt ccaggaacat ctcgacgacg tcttcgtacg aatcgtcgga ttgggtggtg ggccgggcag ctgtaggtgt cggagcgatt gcgttggtca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMT NAIAPTPTAA RPTTQSDDSY EDVVEMFLEL RRMPAESHEY RRQRERIVAR CLPLADHVAS HFARRGEGLD DLVQVARLGL MNAVNRFDPA KGPSFIGFAV PTMMGEVRRY FRDYSWGMRV PRRLRELHVQ ISRTTADLAQ QLGRAPNAGE LSQVLEVPRE EIVECLVAGD AYRLDSLDAP QGADSSGTPR SVADSVGDID PQIEHITNRE ALRVLVATLP HREREVLRMR FFESMTQSQI AERIGVSQMQ VSRILANTLR CLRDQLE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCA ACGCAATCGC TCCGACACCT ACAGCTGCCC GGCCCACCAC CCAATCCGAC GATTCGTACG AAGACGTCGT CGAGATGTTC CTGGAACTGC GCCGGATGCC CGCGGAATCG CACGAGTACC GCCGTCAGCG TGAGCGCATC GTGGCCCGAT GCCTGCCCCT GGCCGATCAC GTGGCCAGCC ATTTCGCCCG CCGCGGCGAG GGTCTCGACG ACTTGGTCCA GGTCGCGCGG CTGGGGCTGA TGAACGCCGT CAACCGCTTC GATCCGGCGA AGGGTCCCAG CTTCATCGGG TTCGCCGTGC CCACCATGAT GGGCGAGGTC CGCCGCTATT TCCGCGACTA CAGCTGGGGG ATGCGGGTGC CCCGGCGGCT GCGCGAGTTG CACGTGCAGA TCAGCAGGAC GACCGCCGAC CTCGCCCAGC AGCTCGGCCG GGCGCCGAAC GCCGGCGAGC TCTCGCAGGT GCTCGAGGTT CCGCGGGAAG AGATCGTCGA ATGCCTGGTC GCCGGCGACG CCTATCGCCT CGACTCGTTG GATGCGCCGC AGGGTGCGGA CAGTTCCGGG ACGCCGCGCT CGGTGGCCGA CTCGGTGGGC GACATCGACC CGCAGATCGA GCACATCACC AACCGGGAAG CGCTGCGGGT CCTGGTCGCC ACCCTGCCGC ACCGCGAACG CGAGGTGTTG CGGATGCGGT TCTTCGAATC CATGACGCAG AGCCAGATAG CCGAGCGGAT CGGCGTCTCG CAGATGCAGG TCTCGCGCAT CCTGGCAAAC ACCCTGCGCT GCCTGCGCGA CCAACTCGAG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for MyavA.00738.a.B1.PW37550
PURIFICATION DATe: 9/1/2014
CONCENTRATION: 3mg/ml
OBSERVED MW: 33kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT NAIAPTPTAA RPTTQSDDSY EDVVEMFLEL RRMPAESHEY RRQRERIVAR CLPLADHVAS HFARRGEGLD DLVQVARLGL MNAVNRFDPA KGPSFIGFAV PTMMGEVRRY FRDYSWGMRV PRRLRELHVQ ISRTTADLAQ QLGRAPNAGE LSQVLEVPRE EIVECLVAGD AYRLDSLDAP QGADSSGTPR SVADSVGDID PQIEHITNRE ALRVLVATLP HREREVLRMR FFESMTQSQI AERIGVSQMQ VSRILANTLR CLRDQLE
Validated NT Sequence
atcttactca cttactcgag ttggtcgcgc aggcagcgca gggtgtttgc caggatgcgc gagacctgca tctgcgagac gccgatccgc tcggctatct ggctctgcgt catggattcg aagaaccgca tccgcaacac ctcgcgttcg cggtgcggca gggtggcgac caggacccgc agcgcttccc ggttggtgat gtgctcgatc tgcgggtcga tgtcgcccac cgagtcggcc accgagcgcg gcgtcccgga actgtccgca ccctgcggcg catccaacga gtcgaggcga taggcgtcgc cggcgaccag gcattcgacg atctcttccc gcggaacctc gagcacctgc gagagctcgc cggcgttcgg cgcccggccg agctgctggg cgaggtcggc ggtcgtcctg ctgatctgca cgtgcaactc gcgcagccgc cggggcaccc gcatccccca gctgtagtcg cggaaatagc ggcggacctc gcccatcatg gtgggcacgg cgaacccgat gaagctggga cccttcgccg gatcgaagcg gttgacggcg ttcatcagcc ccagccgcgc gacctggacc aagtcgtcga gaccctcgcc gcggcgggcg aaatggctgg ccacgtgatc ggccaggggc aggcatcggg ccacgatgcg ctcacgctga cggcggtact cgtgcgattc cgcgggcatc cggcgcagtt ccaggaacat ctcgacgacg tcttcgtacg aatcgtcgga ttgggtggtg ggccgggcag ctgtaggtgt cggagcgatt gcgttggtca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMT NAIAPTPTAA RPTTQSDDSY EDVVEMFLEL RRMPAESHEY RRQRERIVAR CLPLADHVAS HFARRGEGLD DLVQVARLGL MNAVNRFDPA KGPSFIGFAV PTMMGEVRRY FRDYSWGMRV PRRLRELHVQ ISRTTADLAQ QLGRAPNAGE LSQVLEVPRE EIVECLVAGD AYRLDSLDAP QGADSSGTPR SVADSVGDID PQIEHITNRE ALRVLVATLP HREREVLRMR FFESMTQSQI AERIGVSQMQ VSRILANTLR CLRDQLE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCA ACGCAATCGC TCCGACACCT ACAGCTGCCC GGCCCACCAC CCAATCCGAC GATTCGTACG AAGACGTCGT CGAGATGTTC CTGGAACTGC GCCGGATGCC CGCGGAATCG CACGAGTACC GCCGTCAGCG TGAGCGCATC GTGGCCCGAT GCCTGCCCCT GGCCGATCAC GTGGCCAGCC ATTTCGCCCG CCGCGGCGAG GGTCTCGACG ACTTGGTCCA GGTCGCGCGG CTGGGGCTGA TGAACGCCGT CAACCGCTTC GATCCGGCGA AGGGTCCCAG CTTCATCGGG TTCGCCGTGC CCACCATGAT GGGCGAGGTC CGCCGCTATT TCCGCGACTA CAGCTGGGGG ATGCGGGTGC CCCGGCGGCT GCGCGAGTTG CACGTGCAGA TCAGCAGGAC GACCGCCGAC CTCGCCCAGC AGCTCGGCCG GGCGCCGAAC GCCGGCGAGC TCTCGCAGGT GCTCGAGGTT CCGCGGGAAG AGATCGTCGA ATGCCTGGTC GCCGGCGACG CCTATCGCCT CGACTCGTTG GATGCGCCGC AGGGTGCGGA CAGTTCCGGG ACGCCGCGCT CGGTGGCCGA CTCGGTGGGC GACATCGACC CGCAGATCGA GCACATCACC AACCGGGAAG CGCTGCGGGT CCTGGTCGCC ACCCTGCCGC ACCGCGAACG CGAGGTGTTG CGGATGCGGT TCTTCGAATC CATGACGCAG AGCCAGATAG CCGAGCGGAT CGGCGTCTCG CAGATGCAGG TCTCGCGCAT CCTGGCAAAC ACCCTGCGCT GCCTGCGCGA CCAACTCGAG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT