MyavA.00665.a

ABC transporter ATP-binding subunit

CENTER ID: MyavA.00665.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00665.a.A1.GE29004 Full length( MyavA.00665.a ) 1 253
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.3017
RefSeq: YP_882269.1
UniProt: A0A0H2ZTA0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMSSSGDELI GGAEPAVRIE HLRVVRGKRP ALHDFSVQIA KGSITGLLGP SGCGKTTLIR CIVGTQILTK GSVTVLGKPA GSAELRHRVG YLPQDPTIYN DLRVVDNVRY FAALYGFDGQ AADAAIDRVG LTDHRIALCG NLSGGQRTRV SLACALVRRP DLLVLDEPTV GLDPVLRADL WDQFAALARG GTTLLVSSHV MDEADHCGDL LLMREGRLVA HTTPNRLRED TGCTSLEDAF LSIIKRSTMR EAG
NT Sequence
ATGATGAGTT CATCCGGTGA TGAATTAATC GGCGGGGCCG AACCGGCGGT GCGCATCGAG CACCTGCGGG TGGTCCGCGG CAAACGCCCG GCCCTGCACG ACTTCTCCGT GCAGATCGCC AAGGGCAGCA TCACCGGGCT GCTCGGGCCG TCCGGCTGCG GCAAGACCAC CCTGATCCGC TGCATCGTGG GCACCCAGAT CCTCACCAAG GGCAGCGTCA CGGTGCTCGG AAAACCCGCC GGCAGCGCCG AGCTGCGCCA CCGGGTCGGC TACCTGCCGC AGGACCCGAC CATCTACAAC GACCTGCGGG TGGTGGACAA CGTCCGCTAC TTCGCGGCGC TGTACGGGTT CGACGGCCAG GCCGCCGACG CCGCCATCGA TCGGGTGGGG CTGACCGATC ATCGAATTGC CTTGTGCGGC AACCTGTCCG GTGGTCAGCG CACCCGGGTG TCGCTGGCCT GCGCGCTGGT GCGCCGGCCC GACCTGCTGG TGCTGGACGA ACCCACCGTG GGCCTGGATC CGGTGCTGCG CGCCGACCTG TGGGATCAGT TCGCCGCGCT GGCCCGCGGC GGGACCACGC TGCTGGTGTC CAGCCATGTG ATGGACGAGG CCGACCACTG CGGCGACCTG CTGCTGATGC GGGAAGGGCG GCTGGTCGCC CACACCACCC CGAACCGACT GCGAGAGGAC ACCGGATGCA CGTCACTGGA GGACGCGTTT CTGTCCATCA TCAAGCGCAG CACCATGCGC GAAGCCGGC
Details for MyavA.00665.a.A1.GE29004
HARVESTED ON: 4/9/2010
SEQUENCED ON: 4/29/2010
EXPECTED MW: 29kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 15
PERCENT COVERAGE: 56
Validated AA Sequence
MAGHRXRGPA AGQRXELIPX XXXXXRXQXX XGFXQHQXXG PAXXXXGQRH PGALXXXKVX AQGNSMIXXX HPIDXXXGGL XVEXVQRXXV XXVVHHPQVX XMVXSCXXXX XRXXXSXCXX XFXXXXXCXX XXSXXXXXSX XXXXXXXXTX XXXX
Validated NT Sequence
tnnttnnnnn nnngttagca gccggnncct cgannnnttg nntgcagaan ttgttcgtgn tgnnnnnnnn nnnnnttcnc gcatggngct gcgcttgatg atggacnnnn nnnnnncctc cagtgacgtg catccggtgt cctctcgcag tcggttcggg gtggtgtggg cgaccagccg cccttcccgc atnaananca ggttgccgca ntggncggnc tcntcnatca catggctgga caccggnagc gtggtcccgc cgcgggccag cgcgnngaac tgatcccaca nnnnnnnncg nnnnnncggn tccaggncca nnnnggnttc ntccagcacc agnaggncgg gccggcacnn cannnnncag gccagcgaca cccgggtgcg ctgancannn naaaggttgn tgcncaaggn aattcaatga tcgnncnnnc ccacccgatc gatgnnnnnn ncggcggcct ggncgtcgaa nccgtacagc gncncnaagt anngnangtt gtccaccacc cncaggtcnn tganatggtc nnntcntgcn tcangnannn nanccggngn ngcanctcnn cctgcnnnnn nnnnttccna nnnncnngan nntgtcnttg nngangatct gnnnnnnnnc natnnagcgn nnnnnnnngn ncntgnnnnn nnnngacgnn annanccngn nnga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMSSSGDEL IGGAEPAVRI EHLRVVRGKR PALHDFSVQI AKGSITGLLG PSGCGKTTLI RCIVGTQILT KGSVTVLGKP AGSAELRHRV GYLPQDPTIY NDLRVVDNVR YFAALYGFDG QAADAAIDRV GLTDHRIALC GNLSGGQRTR VSLACALVRR PDLLVLDEPT VGLDPVLRAD LWDQFAALAR GGTTLLVSSH VMDEADHCGD LLLMREGRLV AHTTPNRLRE DTGCTSLEDA FLSIIKRSTM REAG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatgagttca tccggtgatg aattaatcgg cggggccgaa ccggcggtgc gcatcgagca cctgcgggtg gtccgcggca aacgcccggc cctgcacgac ttctccgtgc agatcgccaa gggcagcatc accgggctgc tcgggccgtc cggctgcggc aagaccaccc tgatccgctg catcgtgggc acccagatcc tcaccaaggg cagcgtcacg gtgctcggaa aacccgccgg cagcgccgag ctgcgccacc gggtcggcta cctgccgcag gacccgacca tctacaacga cctgcgggtg gtggacaacg tccgctactt cgcggcgctg tacgggttcg acggccaggc cgccgacgcc gccatcgatc gggtggggct gaccgatcat cgaattgcct tgtgcggcaa cctgtccggt ggtcagcgca cccgggtgtc gctggcctgc gcgctggtgc gccggcccga cctgctggtg ctggacgaac ccaccgtggg cctggatccg gtgctgcgcg ccgacctgtg ggatcagttc gccgcgctgg cccgcggcgg gaccacgctg ctggtgtcca gccatgtgat ggacgaggcc gaccactgcg gcgacctgct gctgatgcgg gaagggcggc tggtcgccca caccaccccg aaccgactgc gagaggacac cggatgcacg tcactggagg acgcgtttct gtccatcatc aagcgcagca ccatgcgcga agccggcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa