MyavA.00629.f

2,3-dihydroxybenzoate-AMP ligase (EC 6.3.2.-)

CENTER ID: MyavA.00629.f
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00629.f.B2.GE40018 ordered domain 14 542
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|243243.7.peg.1958
RefSeq: WP_011724530.1
UniProt: A0A0H2ZQI8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSPNTTPPAG VLDGFVPFPA ERAAAYRAAG LWTGRALDTI LTDAARRWPD RTAVLDASGG TGFSYAGLDE QANRAAAGLA DAGIAPGDRV LLQLPNGCQF AVALFALLRA GAIPVMCLPG HRAAELGHFA ALSQATALLI ADTAAGFDYR TMAAGLVEEH EALAHVIVDG DPGPFLSWAQ LCERAPAGRP ATPVDPGSPA LLLVSGGTTG TPKLIPRTHD DYVFNANASA ELCGLTRDDV YLAVLSAGHN FPLACPGLLG AMTVGATTVF GTDPSPEAAF ATIARHGVTV TALVPALAKL WAHACEWEDN PPKSLRLLQV GGAKLEADDA RVIRSALTPG LQQVFGMAEG LLNYTRLDDP PEITEHTQGR PLSTADEVRV VDAAGNPVAP GQEGELLVRG PYTFNGYFRA ERDNQRCFDA QGFFRSGDLV RVRDDGYLVV TGRVKDVICR GGETISAADL EEQMLSHPAI FSAAAVPLPD PYLGEKICAA VVFEGPALSL AELNGYLDER GVAAHARPDV LAAMPSLPTT PIGKIDKKAI ANQVSARNRR P
NT Sequence
atgagcccca acaccacgcc gccggccgga gtcctcgacg ggttcgtgcc gtttcccgcc gagcgcgccg ccgcctaccg ggcggccggt ttgtggacgg ggcgagccct ggacacgatc ctgaccgacg cggcccggcg gtggcccgac cgaaccgccg tcctcgacgc ctccggcggc accggtttca gctacgcggg cctcgacgag caggctaacc gcgccgccgc gggtttggcg gacgcgggta tcgcccccgg cgaccgggtg ctgctgcagc tgccgaacgg ctgccagttc gcggtggcgc tgttcgcgct gttacgggcg ggggcgatcc cggtgatgtg cctgcccggc caccgcgccg ccgagcttgg gcatttcgcc gcgctcagcc aggccaccgc actgctgatc gccgacaccg cagcgggttt cgattatcga acgatggccg cggggctcgt cgaggagcat gaagcgctcg cccacgtgat cgtcgacggc gacccggggc cgtttctgtc ctgggcgcag ctgtgcgagc gtgcgcccgc ggggcggccg gcgacgccgg tcgatccggg atcgcctgcg ctgcttctgg tttccggtgg caccaccggc acaccaaagc tcatcccgcg cacccacgac gattacgtct tcaacgccaa cgccagcgcc gaactgtgcg ggctcacccg cgacgacgtc tatctggcgg tgctctcggc cggccacaat ttcccgctcg cctgccccgg cctgctcggc gcgatgacgg tgggcgcgac cacggtgttc ggcaccgacc ccagccccga ggcggccttt gccaccatcg cgcgccacgg cgtcacggtg accgccctgg tcccggccct ggccaaacta tgggcgcacg cctgcgaatg ggaggacaat ccaccgaagt ccttgcggct cttgcaagtc ggcggcgcca agttggaggc cgacgacgcc cgtgtgatcc gcagcgcgtt gacgccgggc ctgcagcagg tgttcgggat ggccgagggc ctgctgaact acacccggct cgacgatcca ccggagatca ccgagcacac ccagggacgg ccgttgtcga ccgccgacga ggtgcgggtg gtcgacgcgg cgggcaatcc cgttgccccg gggcaggaag gcgaactgct ggtccgcgga ccgtacacgt tcaacggtta tttccgggcc gaacgcgaca atcaacgctg cttcgacgcg cagggctttt tccgcagcgg cgatttggtc cgggtccgcg atgatggcta cctggtggtg accggacggg tcaaggacgt gatctgccgt ggcggcgaga cgatttccgc ggccgacctc gaggagcaga tgctgagcca cccggcgatc ttctcggccg ccgcagtacc cctccccgac ccctacctcg gcgaaaagat ctgtgccgct gtcgttttcg agggcccggc gctgtcactc gcggagttga acggctacct ggacgagcgc ggtgtggccg cgcacgcccg tcccgacgtc ctggccgcga tgccgtcatt gcccaccacg ccgatcggca agatcgacaa aaaggcgatc gcgaaccagg tatcggcgcg gaatcggcgt ccctga
Details for MyavA.00629.f.B2.GE40018
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 56kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHMGF VPFPAERAAA YRAAGLWTGR ALDTILTDAA RRWPDRTAVL DASGGTGFSY AGLDEQANRA AAGLADAGIA PGDRVLLQLP NGCQFAVALF ALLRAGAIPV MCLPGHRAAE LGHFAALSQA TALLIADTAA GFDYRTMAAG LVEEHEALAH VIVDGDPGPF LSWAQLCERA PAGRPATPVD PGSPALLLVS GGTTGTPKLI PRTHDDYVFN ANASAELCGL TRDDVYLAVL SAGHNFPLAC PGLLGAMTVG ATTVFGTDPS PEAAFATIAR HGVTVTALVP ALAKLWAHAC EWEDNPPKSL RLLQVGGAKL EADDARVIRS ALTPGLQQVF GMAEGLLNYT RLDDPPEITE HTQGRPLSTA DEVRVVDAAG NPVAPGQEGE LLVRGPYTFN GYFRAERDNQ RCFDAQGFFR SGDLVRVRDD GYLVVTGRVK DVICRGGETI SAADLEEQML SHPAIFSAAA VPLPDPYLGE KICAAVVFEG PALSLAELNG YLDERGVAAH ARPDVLAAMP SLPTTPIG
Validated NT Sequence
cttgccgatc ggcgtggtgg gcaatgacgg catcgcggcc aggacgtcgg gacgggcgtg cgcggccaca ccgcgctcgt ccaggtagcc gttcaactcc gcgagtgaca gcgccgggcc ctcgaaaacg acagcggcac agatcttttc gccgaggtag gggtcgggga ggggtactgc ggcggccgag aagatcgccg ggtggctcag catctgctcc tcgaggtcgg ccgcggaaat cgtctcgccg ccacggcaga tcacgtcctt gacccgtccg gtcaccacca ggtagccatc atcgcggacc cggaccaaat cgccgctgcg gaaaaagccc tgcgcgtcga agcagcgttg attgtcgcgt tcggcccgga aataaccgtt gaacgtgtac ggtccgcgga ccagcagttc gccttcctgc cccggggcaa cgggattgcc cgccgcgtcg accacccgca cctcgtcggc ggtcgacaac ggccgtccct gggtgtgctc ggtgatctcc ggtggatcgt cgagccgggt gtagttcagc aggccctcgg ccatcccgaa cacctgctgc aggcccggcg tcaacgcgct gcggatcaca cgggcgtcgt cggcctccaa cttggcgccg ccgacttgca agagccgcaa ggacttcggt ggattgtcct cccattcgca ggcgtgcgcc catagtttgg ccagggccgg gaccagggcg gtcaccgtga cgccgtggcg cgcgatggtg gcaaaggccg cctcggggct ggggtcggtg ccgaacaccg tggtcgcgcc caccgtcatc gcgccgagca ggccggggca ggcgagcggg aaattgtggc cggccgagag caccgccaga tagacgtcgt cgcgggtgag cccgcacagt tcggcgctgg cgttggcgtt gaagacgtaa tcgtcgtggg tgcgcgggat gagctttggt gtgccggtgg tgccaccgga aaccagaagc agcgcaggcg atcccggatc gaccggcgtc gccggccgcc ccgcgggcgc acgctcgcac agctgcgccc aggacagaaa cggccccggg tcgccgtcga cgatcacgtg ggcgagcgct tcatgctcct cgacgagccc cgcggccatc gttcgataat cgaaacccgc tgcggtgtcg gcgatcagca gtgcggtggc ctggctgagc gcggcgaaat gcccaagctc ggcggcgcgg tggccgggca ggcacatcac cgggatcgcc cccgcccgta acagcgcgaa cagcgccacc gcgaactggc agccgttcgg cagctgcagc agcacccggt cgccgggggc gatacccgcg tccgccaaac ccgcggcggc gcggttagcc tgctcgtcga ggcccgcgta gctgaaaccg gtgccgccgg aggcgtcgag gacggcggtt cggtcgggcc accgccgggc cgcgtcggtc aggatcgtgt ccagggctcg ccccgtccac aaaccggccg cccggtaggc ggcggcgcgc tcggcgggaa acggcacgaa ccccatatgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHGF VPFPAERAAA YRAAGLWTGR ALDTILTDAA RRWPDRTAVL DASGGTGFSY AGLDEQANRA AAGLADAGIA PGDRVLLQLP NGCQFAVALF ALLRAGAIPV MCLPGHRAAE LGHFAALSQA TALLIADTAA GFDYRTMAAG LVEEHEALAH VIVDGDPGPF LSWAQLCERA PAGRPATPVD PGSPALLLVS GGTTGTPKLI PRTHDDYVFN ANASAELCGL TRDDVYLAVL SAGHNFPLAC PGLLGAMTVG ATTVFGTDPS PEAAFATIAR HGVTVTALVP ALAKLWAHAC EWEDNPPKSL RLLQVGGAKL EADDARVIRS ALTPGLQQVF GMAEGLLNYT RLDDPPEITE HTQGRPLSTA DEVRVVDAAG NPVAPGQEGE LLVRGPYTFN GYFRAERDNQ RCFDAQGFFR SGDLVRVRDD GYLVVTGRVK DVICRGGETI SAADLEEQML SHPAIFSAAA VPLPDPYLGE KICAAVVFEG PALSLAELNG YLDERGVAAH ARPDVLAAMP SLPTTPIGKI DKKAIAN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgggttcg tgccgtttcc cgccgagcgc gccgccgcct accgggcggc cggtttgtgg acggggcgag ccctggacac gatcctgacc gacgcggccc ggcggtggcc cgaccgaacc gccgtcctcg acgcctccgg cggcaccggt ttcagctacg cgggcctcga cgagcaggct aaccgcgccg ccgcgggttt ggcggacgcg ggtatcgccc ccggcgaccg ggtgctgctg cagctgccga acggctgcca gttcgcggtg gcgctgttcg cgctgttacg ggcgggggcg atcccggtga tgtgcctgcc cggccaccgc gccgccgagc ttgggcattt cgccgcgctc agccaggcca ccgcactgct gatcgccgac accgcagcgg gtttcgatta tcgaacgatg gccgcggggc tcgtcgagga gcatgaagcg ctcgcccacg tgatcgtcga cggcgacccg gggccgtttc tgtcctgggc gcagctgtgc gagcgtgcgc ccgcggggcg gccggcgacg ccggtcgatc cgggatcgcc tgcgctgctt ctggtttccg gtggcaccac cggcacacca aagctcatcc cgcgcaccca cgacgattac gtcttcaacg ccaacgccag cgccgaactg tgcgggctca cccgcgacga cgtctatctg gcggtgctct cggccggcca caatttcccg ctcgcctgcc ccggcctgct cggcgcgatg acggtgggcg cgaccacggt gttcggcacc gaccccagcc ccgaggcggc ctttgccacc atcgcgcgcc acggcgtcac ggtgaccgcc ctggtcccgg ccctggccaa actatgggcg cacgcctgcg aatgggagga caatccaccg aagtccttgc ggctcttgca agtcggcggc gccaagttgg aggccgacga cgcccgtgtg atccgcagcg cgttgacgcc gggcctgcag caggtgttcg ggatggccga gggcctgctg aactacaccc ggctcgacga tccaccggag atcaccgagc acacccaggg acggccgttg tcgaccgccg acgaggtgcg ggtggtcgac gcggcgggca atcccgttgc cccggggcag gaaggcgaac tgctggtccg cggaccgtac acgttcaacg gttatttccg ggccgaacgc gacaatcaac gctgcttcga cgcgcagggc tttttccgca gcggcgattt ggtccgggtc cgcgatgatg gctacctggt ggtgaccgga cgggtcaagg acgtgatctg ccgtggcggc gagacgattt ccgcggccga cctcgaggag cagatgctga gccacccggc gatcttctcg gccgccgcag tacccctccc cgacccctac ctcggcgaaa agatctgtgc cgctgtcgtt ttcgagggcc cggcgctgtc actcgcggag ttgaacggct acctggacga gcgcggtgtg gccgcgcacg cccgtcccga cgtcctggcc gcgatgccgt cattgcccac cacgccgatc ggcaagatcg acaaaaaggc gatcgcgaac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat