MyavA.00612.a

Lysine--tRNA ligase LysS (EC 6.1.1.6) (Lysyl-tRNA synthetase)

CENTER ID: MyavA.00612.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00612.a.B1.GE40744 full length 1 499
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: A0A0H3A179

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSPAPDADIP EQYRIRRDKR ARLLAEGHDP YPVAVERTHT LAQVRAAHPD LPVDTATDDV VGVAGRVIFA RNSGKLCFAT LQEGDGTTLQ VMVSLDKVGR EALDAWKADV DLGDIVSVHG TLISSRRGEL SVLADSWRMA SKSLRPLPVA HKEMSEEARV RQRYVDLIVR PQARTVARQR IAVIRAIRNA LERRGFLEVE TPMLQTLAGG AAARPFVTHS NALDIDLYLR IAPELFLKRC VVGGFDKVFE LNRVFRNEGA DSTHSPEFSM LETYQAYGTY DDSAVVTREL IQEVADEAIG TRQLPLPDGS VYDIDGEWAS IEMYPSLSAA LGEEITPQTT VEKLLGIAER LGVEIPRDRG YGHGKIVEEL WEHTVGDTLT APTFVRDFPV ETTPLTRQHR SIDGVTEKWD LYVRGVELAT GYSELTDPVV QRERFAAQAR AAAAGDDEAM VLDEDFLAAL EYGMPPCTGT GMGIDRLLMT LTGLSIRETV LFPIVRPHS
NT Sequence
gtgagtcccg ccccagacgc cgacattccc gagcagtacc ggatccgccg ggacaagcgc gcgcggctgc tcgccgaggg gcacgacccg tacccggtcg ccgtcgaacg cacccacacg ctggcgcagg tgcgcgccgc ccaccccgac ctgcccgtcg acaccgcgac cgacgacgtg gtcggcgtcg ccggccgggt gatcttcgcg cgcaactccg gcaagctgtg cttcgccacg ctgcaggagg gcgacggcac caccctgcag gtgatggtca gcctggacaa ggtgggccgc gaagccctgg acgcgtggaa ggccgacgtc gacctgggcg acatcgtctc cgtgcacggc accctgatca gctcccggcg cggcgaactg tccgtgctcg ccgactcgtg gcggatggcg tccaagtcgt tgcgtccgct gcccgtcgcg cacaaggaga tgagcgaaga ggcgcgggtg cggcagcgct acgtcgacct gatcgtgcgc ccgcaggcgc gcacggtcgc ccggcagcgg atcgcggtga tccgggcgat ccgcaacgcc ttggaacggc gcgggtttct cgaagtcgaa accccaatgt tgcagacgct ggccgggggc gcggctgcac ggccgttcgt cacccattcc aacgcgctcg acatcgatct ctacctgcgg atcgccccgg aactgttcct caagcgctgc gtcgtcggtg gtttcgataa ggttttcgaa ctaaatcgcg tgttccgaaa cgaaggtgcg gattccaccc attctccgga attctccatg ctggagacct accaggccta cggaacgtat gacgattcgg cggtggtgac gcgcgagctt attcaagagg tggccgacga ggccatcgga acccgacaac taccgctgcc cgacggcagc gtctacgaca tagacggaga atgggcttct atagaaatgt acccgtcgct gtcggcggcg ctcggtgagg agatcacccc gcagaccacg gtggaaaagc tgctcggcat cgccgagcgg ctgggcgtcg agatcccccg cgaccgcggc tacgggcacg gcaagatcgt cgaggagctg tgggagcaca ccgtgggcga cacgctcacc gcgcccacgt tcgtgcggga tttcccggtc gagacaacgc ctttgacgcg tcagcaccgc agcatcgacg gcgtcaccga gaaatgggac ctgtacgtgc gtggggtcga gctggccacc ggctactcgg agttgaccga tcccgtggtg cagcgcgagc ggttcgccgc ccaggcgcgt gcggcggccg ccggcgacga cgaggccatg gtgctcgacg aagactttct cgccgcgctc gagtatggga tgccgccctg caccggaacg ggaatgggta tcgaccggtt gttgatgaca ttgaccgggc tgtcaattcg ggagacagtt ttgttcccca tcgttcggcc gcattcgtag
Details for MyavA.00612.a.B1.GE40744
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 56kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV SPAPDADIPE QYRIRRDKRA RLLAEGHDPY PVAVERTHTL AQVRAAHPDL PVDTATDDVV GVAGRVIFAR NSGKLCFATL QEGDGTTLQV MVSLDKVGRE ALDAWKADVD LGDIVSVHGT LISSRRGELS VLADSWRMAS KSLRPLPVAH KEMSEEARVR QRYVDLIVRP QARTVARQRI AVIRAIRNAL ERRGFLEVET PMLQTLAGGA AARPFVTHSN ALDIDLYLRI APELFLKRCV VGGFDKVFEL NRVFRNEGAD STHSPEFSML ETYQAYGTYD DSAVVTRELI QEVADEAIGT RQLPLPDGSV YDIDGEWASI EMYPSLSAAL GEEITPQTTV EKLLGIAERL GVEIPRDRGY GHGKIVEELW EHTVGDTLTA PTFVRDFPVE TTPLTRQHRS IDGVTEKWDL YVRGVELATG YSELTDPVVQ RERFAAQARA AAAGDDEAMV LDEDFLAALE YGMPPCTGTG MGIDRLLMTL TGLSIRETVL
Validated NT Sequence
gaacaaaact gtctcccgaa ttgacagccc ggtcaatgtc atcaacaacc ggtcgatacc cattcccgtt ccggtgcagg gcggcatccc atactcgagc gcggcgagaa agtcttcgtc gagcaccatg gcctcgtcgt cgccggcggc cgccgcacgc gcctgggcgg cgaaccgctc gcgctgcacc acgggatcgg tcaactccga gtagccggtg gccagctcga ccccacgcac gtacaggtcc catttctcgg tgacgccgtc gatgctgcgg tgctgacgcg tcaaaggcgt tgtctcgacc gggaaatccc gcacgaacgt gggcgcggtg agcgtgtcgc ccacggtgtg ctcccacagc tcctcgacga tcttgccgtg cccgtagccg cggtcgcggg ggatctcgac gcccagccgc tcggcgatgc cgagcagctt ttccaccgtg gtctgcgggg tgatctcctc accgagcgcc gccgacagcg acgggtacat ttctatagaa gcccattctc cgtctatgtc gtagacgctg ccgtcgggca gcggtagttg tcgggttccg atggcctcgt cggccacctc ttgaataagc tcgcgcgtca ccaccgccga atcgtcatac gttccgtagg cctggtaggt ctccagcatg gagaattccg gagaatgggt ggaatccgca ccttcgtttc ggaacacgcg atttagttcg aaaaccttat cgaaaccacc gacgacgcag cgcttgagga acagttccgg ggcgatccgc aggtagagat cgatgtcgag cgcgttggaa tgggtgacga acggccgtgc agccgcgccc ccggccagcg tctgcaacat tggggtttcg acttcgagaa acccgcgccg ttccaaggcg ttgcggatcg cccggatcac cgcgatccgc tgccgggcga ccgtgcgcgc ctgcgggcgc acgatcaggt cgacgtagcg ctgccgcacc cgcgcctctt cgctcatctc cttgtgcgcg acgggcagcg gacgcaacga cttggacgcc atccgccacg agtcggcgag cacggacagt tcgccgcgcc gggagctgat cagggtgccg tgcacggaga cgatgtcgcc caggtcgacg tcggccttcc acgcgtccag ggcttcgcgg cccaccttgt ccaggctgac catcacctgc agggtggtgc cgtcgccctc ctgcagcgtg gcgaagcaca gcttgccgga gttgcgcgcg aagatcaccc ggccggcgac gccgaccacg tcgtcggtcg cggtgtcgac gggcaggtcg gggtgggcgg cgcgcacctg cgccagcgtg tgggtgcgtt cgacggcgac cgggtacggg tcgtgcccct cggcgagcag ccgcgcgcgc ttgtcccggc ggatccggta ctgctcggga atgtcggcgt ctggggcggg actcaccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMS PAPDADIPEQ YRIRRDKRAR LLAEGHDPYP VAVERTHTLA QVRAAHPDLP VDTATDDVVG VAGRVIFARN SGKLCFATLQ EGDGTTLQVM VSLDKVGREA LDAWKADVDL GDIVSVHGTL ISSRRGELSV LADSWRMASK SLRPLPVAHK EMSEEARVRQ RYVDLIVRPQ ARTVARQRIA VIRAIRNALE RRGFLEVETP MLQTLAGGAA ARPFVTHSNA LDIDLYLRIA PELFLKRCVV GGFDKVFELN RVFRNEGADS THSPEFSMLE TYQAYGTYDD SAVVTRELIQ EVADEAIGTR QLPLPDGSVY DIDGEWASIE MYPSLSAALG EEITPQTTVE KLLGIAERLG VEIPRDRGYG HGKIVEELWE HTVGDTLTAP TFVRDFPVET TPLTRQHRSI DGVTEKWDLY VRGVELATGY SELTDPVVQR ERFAAQARAA AAGDDEAMVL DEDFLAALEY GMPPCTGTGM GIDRLLMTLT GLSIRETVLF PIVRPHS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagtc ccgccccaga cgccgacatt cccgagcagt accggatccg ccgggacaag cgcgcgcggc tgctcgccga ggggcacgac ccgtacccgg tcgccgtcga acgcacccac acgctggcgc aggtgcgcgc cgcccacccc gacctgcccg tcgacaccgc gaccgacgac gtggtcggcg tcgccggccg ggtgatcttc gcgcgcaact ccggcaagct gtgcttcgcc acgctgcagg agggcgacgg caccaccctg caggtgatgg tcagcctgga caaggtgggc cgcgaagccc tggacgcgtg gaaggccgac gtcgacctgg gcgacatcgt ctccgtgcac ggcaccctga tcagctcccg gcgcggcgaa ctgtccgtgc tcgccgactc gtggcggatg gcgtccaagt cgttgcgtcc gctgcccgtc gcgcacaagg agatgagcga agaggcgcgg gtgcggcagc gctacgtcga cctgatcgtg cgcccgcagg cgcgcacggt cgcccggcag cggatcgcgg tgatccgggc gatccgcaac gccttggaac ggcgcgggtt tctcgaagtc gaaaccccaa tgttgcagac gctggccggg ggcgcggctg cacggccgtt cgtcacccat tccaacgcgc tcgacatcga tctctacctg cggatcgccc cggaactgtt cctcaagcgc tgcgtcgtcg gtggtttcga taaggttttc gaactaaatc gcgtgttccg aaacgaaggt gcggattcca cccattctcc ggaattctcc atgctggaga cctaccaggc ctacggaacg tatgacgatt cggcggtggt gacgcgcgag cttattcaag aggtggccga cgaggccatc ggaacccgac aactaccgct gcccgacggc agcgtctacg acatagacgg agaatgggct tctatagaaa tgtacccgtc gctgtcggcg gcgctcggtg aggagatcac cccgcagacc acggtggaaa agctgctcgg catcgccgag cggctgggcg tcgagatccc ccgcgaccgc ggctacgggc acggcaagat cgtcgaggag ctgtgggagc acaccgtggg cgacacgctc accgcgccca cgttcgtgcg ggatttcccg gtcgagacaa cgcctttgac gcgtcagcac cgcagcatcg acggcgtcac cgagaaatgg gacctgtacg tgcgtggggt cgagctggcc accggctact cggagttgac cgatcccgtg gtgcagcgcg agcggttcgc cgcccaggcg cgtgcggcgg ccgccggcga cgacgaggcc atggtgctcg acgaagactt tctcgccgcg ctcgagtatg ggatgccgcc ctgcaccgga acgggaatgg gtatcgaccg gttgttgatg acattgaccg ggctgtcaat tcgggagaca gttttgttcc ccatcgttcg gccgcattcg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat