MyavA.00554.a
3-hydroxyacyl-CoA dehydrogenase type-2
| CENTER ID: | MyavA.00554.a |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.00554.a.A1.GE28552 | Full length( MyavA.00554.a ) | 1 | 259 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.00554.a.A1.PS00625 | Full length( MyavA.00554.a ) | 1 | 259 |
Structures
3PPI
DEPOSITED: 11/24/2010
DETERMINATION: XRay
CLONE: MyavA.00554.a.A1.GE28552
PROTEIN: MyavA.00554.a.A1.PS00625
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129031 |
| PATRIC ID: | fig|243243.7.peg.1768 |
| RefSeq: | YP_881035.1 |
| UniProt: | A0A0H3A4S5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIKQFEGAS AIVSGGAGGL GEATVRRLHA DGLGVVIADL AAEKGKALAD ELGNRAEFVS TNVTSEDSVL AAIEAANQLG RLRYAVVAHG GFGVAQRIVQ RDGSPADMGG FTKTIDLYLN GTYNVARLVA ASIAAAEPRE NGERGALVLT ASIAGYEGQI GQTAYAAAKA GVIGLTIAAA RDLSSAGIRV NTIAPGTMKT PIMESVGEEA LAKFAANIPF PKRLGTPDEF ADAAAFLLTN GYINGEVMRL DGAQRFTPK
NT Sequence
GTGACAATCA AGCAATTCGA AGGCGCCTCG GCGATCGTCA GTGGCGGCGC GGGCGGTTTG GGTGAGGCCA CCGTGCGGCG GTTGCACGCC GACGGGCTCG GCGTGGTCAT CGCCGACCTC GCCGCCGAGA AGGGCAAGGC ACTGGCCGAT GAGCTGGGCA ACCGGGCCGA GTTCGTCAGC ACCAATGTAA CGAGCGAGGA CAGCGTCCTC GCTGCGATCG AGGCGGCCAA TCAGCTTGGC CGGCTGCGCT ATGCGGTGGT CGCGCACGGC GGCTTCGGCG TCGCCCAACG GATCGTGCAG CGCGACGGCA GCCCGGCCGA CATGGGCGGC TTCACCAAGA CGATCGACCT CTACCTCAAC GGCACCTACA ACGTGGCGCG ACTGGTAGCG GCCTCGATCG CGGCAGCCGA GCCCCGCGAG AACGGCGAAC GGGGTGCACT CGTGTTGACC GCTTCTATCG CGGGCTACGA GGGCCAGATC GGGCAGACCG CATACGCTGC GGCCAAGGCG GGGGTGATCG GGTTGACCAT CGCGGCGGCC CGCGACTTGA GCTCGGCGGG TATCAGAGTC AACACCATCG CCCCGGGAAC GATGAAGACA CCGATCATGG AATCGGTCGG CGAGGAGGCA CTGGCCAAGT TCGCGGCCAA CATCCCGTTC CCGAAGCGCT TGGGCACACC GGACGAATTC GCCGACGCTG CAGCCTTTTT GCTCACCAAC GGTTACATCA ACGGTGAGGT CATGCGACTC GACGGCGCGC AGCGCTTCAC ACCCAAG
Details for MyavA.00554.a.A1.GE28552
| HARVESTED ON: | 2/9/2010 |
| SEQUENCED ON: | 2/22/2010 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTIKQFEG ASAIVSGGAG GLGEATVRRL HADGLGVVIA DLAAEKGKAL ADELGNRAEF VSTNVTSEDS VLAAIEAANQ LGRLRYAVVA HGGFGVAQRI VQRDGSPADM GGFTKTIDLY LNGTYNVARL VAASIAAAEP RENGERGALV LTASIAGYEG QIGQTAYAAA KAGVIGLTIA AARDLSSAGI RVNTIAPGTM KTPIMESVGE EALAKFAANI PFPKRLGTPD EFADAAAFLL TNGYINGEVM RLDGAQRFTP K
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga caatcaagca attcgaaggc gcctcggcga tcgtcagtgg cggcgcgggc ggtttgggtg aggccaccgt gcggcggttg cacgccgacg ggctcggcgt ggtcatcgcc gacctcgccg ccgagaaggg caaggcactg gccgatgagc tgggcaaccg ggccgagttc gtcagcacca atgtaacgag cgaggacagc gtcctcgctg cgatcgaggc ggccaatcag cttggccggc tgcgctatgc ggtggtcgcg cacggcggct tcggcgtcgc ccaacggatc gtgcagcgcg acggcagccc ggccgacatg ggcggcttca ccaagacgat cgacctctac ctcaacggca cctacaacgt ggcgcgactg gtagcggcct cgatcgcggc agccgagccc cgcgagaacg gcgaacgggg tgcactcgtg ttgaccgctt ctatcgcggg ctacgagggc cagatcgggc agaccgcata cgctgcggcc aaggcggggg tgatcgggtt gaccatcgcg gcggcccgcg acttgagctc ggcgggtatc agagtcaaca ccatcgcccc gggaacgatg aagacaccga tcatggaatc ggtcggcgag gaggcactgg ccaagttcgc ggccaacatc ccgttcccga agcgcttggg cacaccggac gaattcgccg acgctgcagc ctttttgctc accaacggtt acatcaacgg tgaggtcatg cgactcgacg gcgcgcagcg cttcacaccc aagtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTIKQFEG ASAIVSGGAG GLGEATVRRL HADGLGVVIA DLAAEKGKAL ADELGNRAEF VSTNVTSEDS VLAAIEAANQ LGRLRYAVVA HGGFGVAQRI VQRDGSPADM GGFTKTIDLY LNGTYNVARL VAASIAAAEP RENGERGALV LTASIAGYEG QIGQTAYAAA KAGVIGLTIA AARDLSSAGI RVNTIAPGTM KTPIMESVGE EALAKFAANI PFPKRLGTPD EFADAAAFLL TNGYINGEVM RLDGAQRFTP K
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacaatc aagcaattcg aaggcgcctc ggcgatcgtc agtggcggcg cgggcggttt gggtgaggcc accgtgcggc ggttgcacgc cgacgggctc ggcgtggtca tcgccgacct cgccgccgag aagggcaagg cactggccga tgagctgggc aaccgggccg agttcgtcag caccaatgta acgagcgagg acagcgtcct cgctgcgatc gaggcggcca atcagcttgg ccggctgcgc tatgcggtgg tcgcgcacgg cggcttcggc gtcgcccaac ggatcgtgca gcgcgacggc agcccggccg acatgggcgg cttcaccaag acgatcgacc tctacctcaa cggcacctac aacgtggcgc gactggtagc ggcctcgatc gcggcagccg agccccgcga gaacggcgaa cggggtgcac tcgtgttgac cgcttctatc gcgggctacg agggccagat cgggcagacc gcatacgctg cggccaaggc gggggtgatc gggttgacca tcgcggcggc ccgcgacttg agctcggcgg gtatcagagt caacaccatc gccccgggaa cgatgaagac accgatcatg gaatcggtcg gcgaggaggc actggccaag ttcgcggcca acatcccgtt cccgaagcgc ttgggcacac cggacgaatt cgccgacgct gcagcctttt tgctcaccaa cggttacatc aacggtgagg tcatgcgact cgacggcgcg cagcgcttca cacccaagaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MyavA.00554.a.A1.PS00625
| PURIFICATION DATe: | 6/3/2010 |
| CONCENTRATION: | 44.38mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTIKQFEG ASAIVSGGAG GLGEATVRRL HADGLGVVIA DLAAEKGKAL ADELGNRAEF VSTNVTSEDS VLAAIEAANQ LGRLRYAVVA HGGFGVAQRI VQRDGSPADM GGFTKTIDLY LNGTYNVARL VAASIAAAEP RENGERGALV LTASIAGYEG QIGQTAYAAA KAGVIGLTIA AARDLSSAGI RVNTIAPGTM KTPIMESVGE EALAKFAANI PFPKRLGTPD EFADAAAFLL TNGYINGEVM RLDGAQRFTP K
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga caatcaagca attcgaaggc gcctcggcga tcgtcagtgg cggcgcgggc ggtttgggtg aggccaccgt gcggcggttg cacgccgacg ggctcggcgt ggtcatcgcc gacctcgccg ccgagaaggg caaggcactg gccgatgagc tgggcaaccg ggccgagttc gtcagcacca atgtaacgag cgaggacagc gtcctcgctg cgatcgaggc ggccaatcag cttggccggc tgcgctatgc ggtggtcgcg cacggcggct tcggcgtcgc ccaacggatc gtgcagcgcg acggcagccc ggccgacatg ggcggcttca ccaagacgat cgacctctac ctcaacggca cctacaacgt ggcgcgactg gtagcggcct cgatcgcggc agccgagccc cgcgagaacg gcgaacgggg tgcactcgtg ttgaccgctt ctatcgcggg ctacgagggc cagatcgggc agaccgcata cgctgcggcc aaggcggggg tgatcgggtt gaccatcgcg gcggcccgcg acttgagctc ggcgggtatc agagtcaaca ccatcgcccc gggaacgatg aagacaccga tcatggaatc ggtcggcgag gaggcactgg ccaagttcgc ggccaacatc ccgttcccga agcgcttggg cacaccggac gaattcgccg acgctgcagc ctttttgctc accaacggtt acatcaacgg tgaggtcatg cgactcgacg gcgcgcagcg cttcacaccc aagtaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTIKQFEG ASAIVSGGAG GLGEATVRRL HADGLGVVIA DLAAEKGKAL ADELGNRAEF VSTNVTSEDS VLAAIEAANQ LGRLRYAVVA HGGFGVAQRI VQRDGSPADM GGFTKTIDLY LNGTYNVARL VAASIAAAEP RENGERGALV LTASIAGYEG QIGQTAYAAA KAGVIGLTIA AARDLSSAGI RVNTIAPGTM KTPIMESVGE EALAKFAANI PFPKRLGTPD EFADAAAFLL TNGYINGEVM RLDGAQRFTP K
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACAATC AAGCAATTCG AAGGCGCCTC GGCGATCGTC AGTGGCGGCG CGGGCGGTTT GGGTGAGGCC ACCGTGCGGC GGTTGCACGC CGACGGGCTC GGCGTGGTCA TCGCCGACCT CGCCGCCGAG AAGGGCAAGG CACTGGCCGA TGAGCTGGGC AACCGGGCCG AGTTCGTCAG CACCAATGTA ACGAGCGAGG ACAGCGTCCT CGCTGCGATC GAGGCGGCCA ATCAGCTTGG CCGGCTGCGC TATGCGGTGG TCGCGCACGG CGGCTTCGGC GTCGCCCAAC GGATCGTGCA GCGCGACGGC AGCCCGGCCG ACATGGGCGG CTTCACCAAG ACGATCGACC TCTACCTCAA CGGCACCTAC AACGTGGCGC GACTGGTAGC GGCCTCGATC GCGGCAGCCG AGCCCCGCGA GAACGGCGAA CGGGGTGCAC TCGTGTTGAC CGCTTCTATC GCGGGCTACG AGGGCCAGAT CGGGCAGACC GCATACGCTG CGGCCAAGGC GGGGGTGATC GGGTTGACCA TCGCGGCGGC CCGCGACTTG AGCTCGGCGG GTATCAGAGT CAACACCATC GCCCCGGGAA CGATGAAGAC ACCGATCATG GAATCGGTCG GCGAGGAGGC ACTGGCCAAG TTCGCGGCCA ACATCCCGTT CCCGAAGCGC TTGGGCACAC CGGACGAATT CGCCGACGCT GCAGCCTTTT TGCTCACCAA CGGTTACATC AACGGTGAGG TCATGCGACT CGACGGCGCG CAGCGCTTCA CACCCAAGAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA