MyavA.00416.c

ABC-type molybdenum transport system, ATPase component

CENTER ID: MyavA.00416.c
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00416.c.A1.GE28541 Full length( MyavA.00416.c ) 1 280
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00416.c.A1.PS00577 Full length( MyavA.00416.c ) 1 280

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.3826
RefSeq: YP_883068.1
UniProt: A0A0H3A262

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPENDSAAAD PDLLIELRDV SLRRGGNVLV GPLDWAVELD ERWVIVGPNG AGKTSLLRIA AAAEHPSSGV AFVLGERLGR VDVTELRSRI GLSSSALAQR VPSDEVVRDL VVSAGYAVLG RWRERYEDVD YRRAVDMLES LGAEHLADRS YGTLSEGERK RVLIARALMT DPELLLLDEP AAGLDLGGRE ELVARLADLA ADPDAPALVL VTHHVEEIPP GFSHCMLLSE GRVVAAGLLT DVLTSENLST AFGQAIALDV VDGRYFARRV RTRAAHRRQL
NT Sequence
GTGCCCGAAA ACGACAGTGC GGCAGCCGAC CCCGATCTGC TGATCGAGTT GCGCGACGTG TCGCTGCGGC GCGGCGGCAA CGTCTTGGTC GGGCCGCTGG ACTGGGCGGT CGAACTGGAC GAGCGGTGGG TGATCGTCGG GCCCAACGGG GCCGGCAAGA CCTCGCTGCT GCGGATCGCC GCGGCGGCCG AACACCCTTC GTCGGGCGTG GCTTTCGTGC TCGGCGAGCG GCTGGGCCGG GTCGACGTGA CGGAGTTGCG GTCGCGGATC GGGCTCAGTT CCTCGGCGCT GGCGCAGCGG GTGCCCAGCG ACGAGGTGGT GCGCGATCTG GTCGTGTCGG CCGGCTACGC GGTGCTGGGC CGGTGGCGGG AGCGCTACGA GGACGTCGAC TACCGCCGGG CCGTCGACAT GCTGGAGAGC CTGGGGGCCG AGCACCTGGC CGACCGCAGC TACGGGACGC TGTCCGAAGG CGAGCGCAAG CGGGTGCTGA TCGCCCGCGC GCTGATGACC GACCCCGAGC TGCTGCTGCT CGACGAGCCC GCCGCGGGCC TGGACCTGGG CGGCCGTGAG GAGCTGGTGG CGCGGCTGGC CGACCTGGCC GCCGACCCCG ACGCACCCGC GCTGGTGCTG GTCACCCATC ACGTCGAGGA GATACCGCCG GGTTTCAGTC ACTGCATGCT GCTCTCGGAG GGTCGGGTGG TGGCCGCCGG ACTGCTGACC GACGTGCTCA CCTCGGAGAA TCTGTCCACC GCGTTCGGCC AGGCGATCGC GCTCGACGTC GTCGACGGGC GCTACTTCGC GCGGCGCGTC CGCACCCGCG CAGCCCACCG GAGGCAGCTA
Details for MyavA.00416.c.A1.GE28541
HARVESTED ON: 2/9/2010
SEQUENCED ON: 2/22/2010
EXPECTED MW: 32kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPENDSAA ADPDLLIELR DVSLRRGGNV LVGPLDWAVE LDERWVIVGP NGAGKTSLLR IAAAAEHPSS GVAFVLGERL GRVDVTELRS RIGLSSSALA QRVPSDEVVR DLVVSAGYAV LGRWRERYED VDYRRAVDML ESLGAEHLAD RSYGTLSEGE RKRVLIARAL MTDPELLLLD EPAAGLDLGG REELVARLAD LAADPDAPAL VLVTHHVEEI PPGFSHCMLL SEGRVVAAGL LTDVLTSENL STAFGQAIAL DVVDGRYFAR RVRTRAAHR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc ccgaaaacga cagtgcggca gccgaccccg atctgctgat cgagttgcgc gacgtgtcgc tgcggcgcgg cggcaacgtc ttggtcgggc cgctggactg ggcggtcgaa ctggacgagc ggtgggtgat cgtcgggccc aacggggccg gcaagacctc gctgctgcgg atcgccgcgg cggccgaaca cccttcgtcg ggcgtggctt tcgtgctcgg cgagcggctg ggccgggtcg acgtgacgga gttgcggtcg cggatcgggc tcagttcctc ggcgctggcg cagcgggtgc ccagcgacga ggtggtgcgc gatctggtcg tgtcggccgg ctacgcggtg ctgggccggt ggcgggagcg ctacgaggac gtcgactacc gccgggccgt cgacatgctg gagagcctgg gggccgagca cctggccgac cgcagctacg ggacgctgtc cgaaggcgag cgcaagcggg tgctgatcgc ccgcgcgctg atgaccgacc ccgagctgct gctgctcgac gagcccgccg cgggcctgga cctgggcggc cgtgaggagc tggtggcgcg gctggccgac ctggccgccg accccgacgc acccgcgctg gtgctggtca cccatcacgt cgaggagata ccgccgggtt tcagtcactg catgctgctc tcggagggtc gggtggtggc cgccggactg ctgaccgacg tgctcacctc ggagaatctg tccaccgcgt tcggccaggc gatcgcgctc gacgtcgtcg acgggcgcta cttcgcgcgg cgcgtccgca cccgcgcagc ccaccggagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPENDSAA ADPDLLIELR DVSLRRGGNV LVGPLDWAVE LDERWVIVGP NGAGKTSLLR IAAAAEHPSS GVAFVLGERL GRVDVTELRS RIGLSSSALA QRVPSDEVVR DLVVSAGYAV LGRWRERYED VDYRRAVDML ESLGAEHLAD RSYGTLSEGE RKRVLIARAL MTDPELLLLD EPAAGLDLGG REELVARLAD LAADPDAPAL VLVTHHVEEI PPGFSHCMLL SEGRVVAAGL LTDVLTSENL STAFGQAIAL DVVDGRYFAR RVRTRAAHRR QL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcccgaa aacgacagtg cggcagccga ccccgatctg ctgatcgagt tgcgcgacgt gtcgctgcgg cgcggcggca acgtcttggt cgggccgctg gactgggcgg tcgaactgga cgagcggtgg gtgatcgtcg ggcccaacgg ggccggcaag acctcgctgc tgcggatcgc cgcggcggcc gaacaccctt cgtcgggcgt ggctttcgtg ctcggcgagc ggctgggccg ggtcgacgtg acggagttgc ggtcgcggat cgggctcagt tcctcggcgc tggcgcagcg ggtgcccagc gacgaggtgg tgcgcgatct ggtcgtgtcg gccggctacg cggtgctggg ccggtggcgg gagcgctacg aggacgtcga ctaccgccgg gccgtcgaca tgctggagag cctgggggcc gagcacctgg ccgaccgcag ctacgggacg ctgtccgaag gcgagcgcaa gcgggtgctg atcgcccgcg cgctgatgac cgaccccgag ctgctgctgc tcgacgagcc cgccgcgggc ctggacctgg gcggccgtga ggagctggtg gcgcggctgg ccgacctggc cgccgacccc gacgcacccg cgctggtgct ggtcacccat cacgtcgagg agataccgcc gggtttcagt cactgcatgc tgctctcgga gggtcgggtg gtggccgccg gactgctgac cgacgtgctc acctcggaga atctgtccac cgcgttcggc caggcgatcg cgctcgacgt cgtcgacggg cgctacttcg cgcggcgcgt ccgcacccgc gcagcccacc ggaggcagct aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MyavA.00416.c.A1.PS00577
PURIFICATION DATe: 4/28/2010
CONCENTRATION: 31.96mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPENDSAA ADPDLLIELR DVSLRRGGNV LVGPLDWAVE LDERWVIVGP NGAGKTSLLR IAAAAEHPSS GVAFVLGERL GRVDVTELRS RIGLSSSALA QRVPSDEVVR DLVVSAGYAV LGRWRERYED VDYRRAVDML ESLGAEHLAD RSYGTLSEGE RKRVLIARAL MTDPELLLLD EPAAGLDLGG REELVARLAD LAADPDAPAL VLVTHHVEEI PPGFSHCMLL SEGRVVAAGL LTDVLTSENL STAFGQAIAL DVVDGRYFAR RVRTRAAHR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc ccgaaaacga cagtgcggca gccgaccccg atctgctgat cgagttgcgc gacgtgtcgc tgcggcgcgg cggcaacgtc ttggtcgggc cgctggactg ggcggtcgaa ctggacgagc ggtgggtgat cgtcgggccc aacggggccg gcaagacctc gctgctgcgg atcgccgcgg cggccgaaca cccttcgtcg ggcgtggctt tcgtgctcgg cgagcggctg ggccgggtcg acgtgacgga gttgcggtcg cggatcgggc tcagttcctc ggcgctggcg cagcgggtgc ccagcgacga ggtggtgcgc gatctggtcg tgtcggccgg ctacgcggtg ctgggccggt ggcgggagcg ctacgaggac gtcgactacc gccgggccgt cgacatgctg gagagcctgg gggccgagca cctggccgac cgcagctacg ggacgctgtc cgaaggcgag cgcaagcggg tgctgatcgc ccgcgcgctg atgaccgacc ccgagctgct gctgctcgac gagcccgccg cgggcctgga cctgggcggc cgtgaggagc tggtggcgcg gctggccgac ctggccgccg accccgacgc acccgcgctg gtgctggtca cccatcacgt cgaggagata ccgccgggtt tcagtcactg catgctgctc tcggagggtc gggtggtggc cgccggactg ctgaccgacg tgctcacctc ggagaatctg tccaccgcgt tcggccaggc gatcgcgctc gacgtcgtcg acgggcgcta cttcgcgcgg cgcgtccgca cccgcgcagc ccaccggagg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPENDSAA ADPDLLIELR DVSLRRGGNV LVGPLDWAVE LDERWVIVGP NGAGKTSLLR IAAAAEHPSS GVAFVLGERL GRVDVTELRS RIGLSSSALA QRVPSDEVVR DLVVSAGYAV LGRWRERYED VDYRRAVDML ESLGAEHLAD RSYGTLSEGE RKRVLIARAL MTDPELLLLD EPAAGLDLGG REELVARLAD LAADPDAPAL VLVTHHVEEI PPGFSHCMLL SEGRVVAAGL LTDVLTSENL STAFGQAIAL DVVDGRYFAR RVRTRAAHRR QL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCCCGAA AACGACAGTG CGGCAGCCGA CCCCGATCTG CTGATCGAGT TGCGCGACGT GTCGCTGCGG CGCGGCGGCA ACGTCTTGGT CGGGCCGCTG GACTGGGCGG TCGAACTGGA CGAGCGGTGG GTGATCGTCG GGCCCAACGG GGCCGGCAAG ACCTCGCTGC TGCGGATCGC CGCGGCGGCC GAACACCCTT CGTCGGGCGT GGCTTTCGTG CTCGGCGAGC GGCTGGGCCG GGTCGACGTG ACGGAGTTGC GGTCGCGGAT CGGGCTCAGT TCCTCGGCGC TGGCGCAGCG GGTGCCCAGC GACGAGGTGG TGCGCGATCT GGTCGTGTCG GCCGGCTACG CGGTGCTGGG CCGGTGGCGG GAGCGCTACG AGGACGTCGA CTACCGCCGG GCCGTCGACA TGCTGGAGAG CCTGGGGGCC GAGCACCTGG CCGACCGCAG CTACGGGACG CTGTCCGAAG GCGAGCGCAA GCGGGTGCTG ATCGCCCGCG CGCTGATGAC CGACCCCGAG CTGCTGCTGC TCGACGAGCC CGCCGCGGGC CTGGACCTGG GCGGCCGTGA GGAGCTGGTG GCGCGGCTGG CCGACCTGGC CGCCGACCCC GACGCACCCG CGCTGGTGCT GGTCACCCAT CACGTCGAGG AGATACCGCC GGGTTTCAGT CACTGCATGC TGCTCTCGGA GGGTCGGGTG GTGGCCGCCG GACTGCTGAC CGACGTGCTC ACCTCGGAGA ATCTGTCCAC CGCGTTCGGC CAGGCGATCG CGCTCGACGT CGTCGACGGG CGCTACTTCG CGCGGCGCGT CCGCACCCGC GCAGCCCACC GGAGGCAGCT AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA