MyavA.00358.h

Enoyl-CoA hydratase/isomerase

CENTER ID: MyavA.00358.h
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00358.h.A1.GE28525 Full length( MyavA.00358.h ) 1 276
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00358.h.A1.PS00627 Full length( MyavA.00358.h ) 1 276

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.1805
RefSeq: YP_881071.1
UniProt: A0A0H3A5V6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAHSRDLGV VRYERDGAIA RIVLDWPERA NAQSSEMVSQ VDSCLDEARQ DYGVKVVIVK GSGKGFCAGH VMAPDAYPEF ADSQLHMGSN YQGSKELFLW PTLRYWEFPK PMIAQVHGYA IGGGTYWALL PELTIASEDA YFQMPLVPGL GFAGGETMIE PWVFMNYKRA AEYLYTAQTL TAHEALRMGL VNRVVTRDEL EPVTEALATK IARAPLSTLM ATKTMLVRAW EQMGMRQHLQ LSADVMSVLE NTSDAQALRA ELVARGRLPR DQAGDG
NT Sequence
ATGGCCGCCC ACAGTAGAGA TCTCGGCGTG GTGCGCTACG AGCGCGACGG CGCGATCGCC CGCATCGTGC TCGACTGGCC GGAACGTGCC AACGCTCAGT CCTCGGAAAT GGTGAGCCAG GTCGACAGCT GCCTCGACGA GGCGCGACAG GACTACGGCG TGAAGGTCGT CATCGTCAAG GGCAGCGGAA AGGGTTTCTG TGCCGGCCAC GTCATGGCAC CCGACGCGTA CCCCGAGTTC GCCGACTCGC AGTTGCACAT GGGCAGCAAC TACCAGGGCA GCAAGGAACT CTTTCTGTGG CCCACATTGC GGTACTGGGA GTTCCCGAAG CCGATGATCG CGCAGGTACA CGGGTACGCG ATCGGTGGCG GCACCTACTG GGCGCTACTG CCCGAGCTGA CCATCGCCTC CGAGGACGCC TACTTCCAGA TGCCGCTGGT GCCCGGCCTC GGCTTCGCAG GCGGAGAGAC GATGATCGAG CCATGGGTAT TCATGAACTA CAAGCGGGCC GCCGAATACC TCTACACCGC ACAGACTTTG ACTGCCCATG AGGCGTTGCG GATGGGCTTG GTCAACCGCG TCGTCACCCG CGACGAACTC GAACCGGTGA CGGAAGCGTT GGCGACCAAG ATCGCTCGTG CCCCGCTGTC GACGCTGATG GCGACCAAGA CCATGCTGGT GCGTGCTTGG GAGCAGATGG GAATGCGCCA GCACCTTCAG CTGTCGGCGG ATGTGATGTC GGTGTTGGAG AACACGTCCG ATGCGCAGGC GCTGCGAGCC GAACTGGTCG CGCGTGGCCG GCTTCCCCGC GACCAGGCAG GCGACGGC
Details for MyavA.00358.h.A1.GE28525
HARVESTED ON: 2/9/2010
SEQUENCED ON: 2/22/2010
EXPECTED MW: 32kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAHSRDLG VVRYERDGAI ARIVLDWPER ANAQSSEMVS QVDSCLDEAR QDYGVKVVIV KGSGKGFCAG HVMAPDAYPE FADSQLHMGS NYQGSKELFL WPTLRYWEFP KPMIAQVHGY AIGGGTYWAL LPELTIASED AYFQMPLVPG LGFAGGETMI EPWVFMNYKR AAEYLYTAQT LTAHEALRMG LVNRVVTRDE LEPVTEALAT KIARAPLSTL MATKTMLVRA WEQMGMRQHL QLSADVMSVL ENTSDAQALR AELVARGRLP RDQAG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccg cccacagtag agatctcggc gtggtgcgct acgagcgcga cggcgcgatc gcccgcatcg tgctcgactg gccggaacgt gccaacgctc agtcctcgga aatggtgagc caggtcgaca gctgcctcga cgaggcgcga caggactacg gcgtgaaggt cgtcatcgtc aagggcagcg gaaagggttt ctgtgccggc cacgtcatgg cacccgacgc gtaccccgag ttcgccgact cgcagttgca catgggcagc aactaccagg gcagcaagga actctttctg tggcccacat tgcggtactg ggagttcccg aagccgatga tcgcgcaggt acacgggtac gcgatcggtg gcggcaccta ctgggcgcta ctgcccgagc tgaccatcgc ctccgaggac gcctacttcc agatgccgct ggtgcccggc ctcggcttcg caggcggaga gacgatgatc gagccatggg tattcatgaa ctacaagcgg gccgccgaat acctctacac cgcacagact ttgactgccc atgaggcgtt gcggatgggc ttggtcaacc gcgtcgtcac ccgcgacgaa ctcgaaccgg tgacggaagc gttggcgacc aagatcgctc gtgccccgct gtcgacgctg atggcgacca agaccatgct ggtgcgtgct tgggagcaga tgggaatgcg ccagcacctt cagctgtcgg cggatgtgat gtcggtgttg gagaacacgt ccgatgcgca ggcgctgcga gccgaactgg tcgcgcgtgg ccggcttccc cgcgaccagg caggcg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAHSRDLG VVRYERDGAI ARIVLDWPER ANAQSSEMVS QVDSCLDEAR QDYGVKVVIV KGSGKGFCAG HVMAPDAYPE FADSQLHMGS NYQGSKELFL WPTLRYWEFP KPMIAQVHGY AIGGGTYWAL LPELTIASED AYFQMPLVPG LGFAGGETMI EPWVFMNYKR AAEYLYTAQT LTAHEALRMG LVNRVVTRDE LEPVTEALAT KIARAPLSTL MATKTMLVRA WEQMGMRQHL QLSADVMSVL ENTSDAQALR AELVARGRLP RDQAGDG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgcccac agtagagatc tcggcgtggt gcgctacgag cgcgacggcg cgatcgcccg catcgtgctc gactggccgg aacgtgccaa cgctcagtcc tcggaaatgg tgagccaggt cgacagctgc ctcgacgagg cgcgacagga ctacggcgtg aaggtcgtca tcgtcaaggg cagcggaaag ggtttctgtg ccggccacgt catggcaccc gacgcgtacc ccgagttcgc cgactcgcag ttgcacatgg gcagcaacta ccagggcagc aaggaactct ttctgtggcc cacattgcgg tactgggagt tcccgaagcc gatgatcgcg caggtacacg ggtacgcgat cggtggcggc acctactggg cgctactgcc cgagctgacc atcgcctccg aggacgccta cttccagatg ccgctggtgc ccggcctcgg cttcgcaggc ggagagacga tgatcgagcc atgggtattc atgaactaca agcgggccgc cgaatacctc tacaccgcac agactttgac tgcccatgag gcgttgcgga tgggcttggt caaccgcgtc gtcacccgcg acgaactcga accggtgacg gaagcgttgg cgaccaagat cgctcgtgcc ccgctgtcga cgctgatggc gaccaagacc atgctggtgc gtgcttggga gcagatggga atgcgccagc accttcagct gtcggcggat gtgatgtcgg tgttggagaa cacgtccgat gcgcaggcgc tgcgagccga actggtcgcg cgtggccggc ttccccgcga ccaggcaggc gacggcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MyavA.00358.h.A1.PS00627
PURIFICATION DATe: 5/29/2010
CONCENTRATION: 53.14mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAHSRDLG VVRYERDGAI ARIVLDWPER ANAQSSEMVS QVDSCLDEAR QDYGVKVVIV KGSGKGFCAG HVMAPDAYPE FADSQLHMGS NYQGSKELFL WPTLRYWEFP KPMIAQVHGY AIGGGTYWAL LPELTIASED AYFQMPLVPG LGFAGGETMI EPWVFMNYKR AAEYLYTAQT LTAHEALRMG LVNRVVTRDE LEPVTEALAT KIARAPLSTL MATKTMLVRA WEQMGMRQHL QLSADVMSVL ENTSDAQALR AELVARGRLP RDQAG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccg cccacagtag agatctcggc gtggtgcgct acgagcgcga cggcgcgatc gcccgcatcg tgctcgactg gccggaacgt gccaacgctc agtcctcgga aatggtgagc caggtcgaca gctgcctcga cgaggcgcga caggactacg gcgtgaaggt cgtcatcgtc aagggcagcg gaaagggttt ctgtgccggc cacgtcatgg cacccgacgc gtaccccgag ttcgccgact cgcagttgca catgggcagc aactaccagg gcagcaagga actctttctg tggcccacat tgcggtactg ggagttcccg aagccgatga tcgcgcaggt acacgggtac gcgatcggtg gcggcaccta ctgggcgcta ctgcccgagc tgaccatcgc ctccgaggac gcctacttcc agatgccgct ggtgcccggc ctcggcttcg caggcggaga gacgatgatc gagccatggg tattcatgaa ctacaagcgg gccgccgaat acctctacac cgcacagact ttgactgccc atgaggcgtt gcggatgggc ttggtcaacc gcgtcgtcac ccgcgacgaa ctcgaaccgg tgacggaagc gttggcgacc aagatcgctc gtgccccgct gtcgacgctg atggcgacca agaccatgct ggtgcgtgct tgggagcaga tgggaatgcg ccagcacctt cagctgtcgg cggatgtgat gtcggtgttg gagaacacgt ccgatgcgca ggcgctgcga gccgaactgg tcgcgcgtgg ccggcttccc cgcgaccagg caggcg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAHSRDLG VVRYERDGAI ARIVLDWPER ANAQSSEMVS QVDSCLDEAR QDYGVKVVIV KGSGKGFCAG HVMAPDAYPE FADSQLHMGS NYQGSKELFL WPTLRYWEFP KPMIAQVHGY AIGGGTYWAL LPELTIASED AYFQMPLVPG LGFAGGETMI EPWVFMNYKR AAEYLYTAQT LTAHEALRMG LVNRVVTRDE LEPVTEALAT KIARAPLSTL MATKTMLVRA WEQMGMRQHL QLSADVMSVL ENTSDAQALR AELVARGRLP RDQAGDG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGCCCAC AGTAGAGATC TCGGCGTGGT GCGCTACGAG CGCGACGGCG CGATCGCCCG CATCGTGCTC GACTGGCCGG AACGTGCCAA CGCTCAGTCC TCGGAAATGG TGAGCCAGGT CGACAGCTGC CTCGACGAGG CGCGACAGGA CTACGGCGTG AAGGTCGTCA TCGTCAAGGG CAGCGGAAAG GGTTTCTGTG CCGGCCACGT CATGGCACCC GACGCGTACC CCGAGTTCGC CGACTCGCAG TTGCACATGG GCAGCAACTA CCAGGGCAGC AAGGAACTCT TTCTGTGGCC CACATTGCGG TACTGGGAGT TCCCGAAGCC GATGATCGCG CAGGTACACG GGTACGCGAT CGGTGGCGGC ACCTACTGGG CGCTACTGCC CGAGCTGACC ATCGCCTCCG AGGACGCCTA CTTCCAGATG CCGCTGGTGC CCGGCCTCGG CTTCGCAGGC GGAGAGACGA TGATCGAGCC ATGGGTATTC ATGAACTACA AGCGGGCCGC CGAATACCTC TACACCGCAC AGACTTTGAC TGCCCATGAG GCGTTGCGGA TGGGCTTGGT CAACCGCGTC GTCACCCGCG ACGAACTCGA ACCGGTGACG GAAGCGTTGG CGACCAAGAT CGCTCGTGCC CCGCTGTCGA CGCTGATGGC GACCAAGACC ATGCTGGTGC GTGCTTGGGA GCAGATGGGA ATGCGCCAGC ACCTTCAGCT GTCGGCGGAT GTGATGTCGG TGTTGGAGAA CACGTCCGAT GCGCAGGCGC TGCGAGCCGA ACTGGTCGCG CGTGGCCGGC TTCCCCGCGA CCAGGCAGGC GACGGCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA