SSGCID: Target Page

MyavA.00358.d

Enoyl-CoA hydratase/isomerase family protein

CENTER ID:	MyavA.00358.d
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.00358.d.A1.GE28521	Full length( MyavA.00358.d )		1	268

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.1543
RefSeq:	YP_880815.1
UniProt:	A0A0H3A4C5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPMSAQFDTI LLEVDATDHV ATITLNRPEQ LNAFNRAMCA EMAQAWRLVK LDDAVHAVVL RAAGDRAFSA GLDIKTPYGQ PENIWNHEDP GEALSPKWQK MWKPVVCAVQ GMCTAGAFYF VNESDVVICS QDATFFDSHV SAGLVCALEP VGLMRRIGLG ETLRIALMGN DERVGADTAL RIGLVTEVVP AERLWDRAHE IAATIAAKPP TATAGTVKAI WESLDKPYRA AMEQGLIYTR LGNPLGTAEL AAGRDAGARG AARTPKIR

NT Sequence

ATGCCGATGA GCGCGCAGTT CGACACCATC CTGCTCGAGG TGGACGCCAC CGACCACGTC GCCACCATCA CGCTGAATCG GCCCGAGCAG CTCAACGCGT TCAACCGCGC CATGTGTGCC GAGATGGCTC AGGCGTGGCG CCTGGTCAAG CTCGACGACG CGGTGCACGC GGTGGTGTTG CGGGCCGCCG GCGACCGGGC GTTCAGCGCC GGGCTGGACA TCAAGACGCC GTACGGTCAG CCCGAAAACA TCTGGAACCA CGAGGATCCC GGCGAAGCTC TGAGCCCGAA GTGGCAGAAG ATGTGGAAGC CGGTGGTGTG CGCGGTCCAG GGCATGTGCA CCGCGGGCGC GTTCTACTTC GTCAACGAGT CCGACGTGGT CATCTGCTCG CAGGACGCCA CCTTTTTCGA TTCGCATGTG TCGGCCGGCC TGGTCTGTGC CCTGGAACCG GTCGGCCTGA TGCGTCGCAT CGGGCTCGGC GAGACGTTGC GGATCGCACT GATGGGCAAC GACGAACGGG TCGGGGCGGA CACCGCGCTG CGGATCGGCC TGGTGACGGA AGTGGTTCCC GCCGAACGGC TCTGGGACCG GGCCCACGAG ATCGCGGCGA CCATCGCCGC CAAGCCCCCG ACCGCGACCG CGGGCACGGT GAAGGCGATC TGGGAATCAC TGGACAAGCC GTATCGCGCG GCGATGGAGC AGGGCCTCAT CTACACCCGG CTGGGCAATC CGCTGGGCAC CGCCGAGCTG GCCGCCGGCC GGGACGCCGG TGCACGCGGC GCGGCACGCA CACCGAAGAT CCGC

Details for MyavA.00358.d.A1.GE28521

HARVESTED ON:	2/9/2010
SEQUENCED ON:	2/22/2010
EXPECTED MW:	31kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPMSAQFDT ILLEVDATDH VATITLNRPE QLNAFNRAMC AEMAQAWRLV KLDDAVHAVV LRAAGDRAFS AGLDIKTPYG QPENIWNHED PGEALSPKWQ KMWKPVVCAV QGMCTAGAFY FVNESDVVIC SQDATFFDSH VSAGLVCALE PVGLMRRIGL GETLRIALMG NDERVGADTA LRIGLVTEVV PAERLWDRAH EIAATIAAKP PTATAGTVKA IWESLDKPYR AAMEQGLIYT RLGNPLGTAE LAAGRDAGAR GAARTPKIR

Validated NT Sequence

ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgccgatg agcgcgcagt tcgacaccat cctgctcgag gtggacgcca ccgaccacgt cgccaccatc acgctgaatc ggcccgagca gctcaacgcg ttcaaccgcg ccatgtgtgc cgagatggct caggcgtggc gcctggtcaa gctcgacgac gcggtgcacg cggtggtgtt gcgggccgcc ggcgaccggg cgttcagcgc cgggctggac atcaagacgc cgtacggtca gcccgaaaac atctggaacc acgaggatcc cggcgaagct ctgagcccga agtggcagaa gatgtggaag ccggtggtgt gcgcggtcca gggcatgtgc accgcgggcg cgttctactt cgtcaacgag tccgacgtgg tcatctgctc gcaggacgcc acctttttcg attcgcatgt gtcggccggc ctggtctgtg ccctggaacc ggtcggcctg atgcgtcgca tcgggctcgg cgagacgttg cggatcgcac tgatgggcaa cgacgaacgg gtcggggcgg acaccgcgct gcggatcggc ctggtgacgg aagtggttcc cgccgaacgg ctctgggacc gggcccacga gatcgcggcg accatcgccg ccaagccccc gaccgcgacc gcgggcacgg tgaaggcgat ctgggaatca ctggacaagc cgtatcgcgc ggcgatggag cagggcctca tctacacccg gctgggcaat ccgctgggca ccgccgagct ggccgccggc cgggacgccg gtgcacgcgg cgcggcacgc acaccgaaga tccgctaata a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgatgagc gcgcagttcg acaccatcct gctcgaggtg gacgccaccg accacgtcgc caccatcacg ctgaatcggc ccgagcagct caacgcgttc aaccgcgcca tgtgtgccga gatggctcag gcgtggcgcc tggtcaagct cgacgacgcg gtgcacgcgg tggtgttgcg ggccgccggc gaccgggcgt tcagcgccgg gctggacatc aagacgccgt acggtcagcc cgaaaacatc tggaaccacg aggatcccgg cgaagctctg agcccgaagt ggcagaagat gtggaagccg gtggtgtgcg cggtccaggg catgtgcacc gcgggcgcgt tctacttcgt caacgagtcc gacgtggtca tctgctcgca ggacgccacc tttttcgatt cgcatgtgtc ggccggcctg gtctgtgccc tggaaccggt cggcctgatg cgtcgcatcg ggctcggcga gacgttgcgg atcgcactga tgggcaacga cgaacgggtc ggggcggaca ccgcgctgcg gatcggcctg gtgacggaag tggttcccgc cgaacggctc tgggaccggg cccacgagat cgcggcgacc atcgccgcca agcccccgac cgcgaccgcg ggcacggtga aggcgatctg ggaatcactg gacaagccgt atcgcgcggc gatggagcag ggcctcatct acacccggct gggcaatccg ctgggcaccg ccgagctggc cgccggccgg gacgccggtg cacgcggcgc ggcacgcaca ccgaagatcc gcaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa