MyavA.00358.b

Enoyl-CoA hydratase

CENTER ID: MyavA.00358.b
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00358.b.A1.GE28519 Full length( MyavA.00358.b ) 1 311
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.651
RefSeq: YP_879949.1
UniProt: A0A0H2ZXL2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTLSEQAGAQ PGARPAEELV TYETLDEGRI ARIWLNRPDA HNAQSRGLLV QLDEAFVRAE ADDAVRVVIL AARGKNFSAG HDLGSELALA ERQPGPGQHP TFRFNGATRD PIAEKTYLQE WHYFFQNTCR WRDLRKITIA QVQGNAISAG LMLIWACDLI VAADNAKFSD VVGVRMGMPG VEYYAHPWEF GPRKAKELLL TGDALDADEA YRLGMVSKVF PADELADKTL EFARRIAERP TMAALLIKDS VNAASDAMGF TEALRHAFHI HELGHAHWAA HNENRYPVGL PPDVEDWRNA KPTRLARRDT P
NT Sequence
ATGACTCTCT CCGAACAAGC GGGGGCGCAG CCCGGCGCCC GGCCGGCCGA GGAGCTGGTG ACCTACGAAA CCCTCGACGA GGGCCGCATC GCGCGCATCT GGCTCAACCG GCCCGACGCC CACAACGCGC AGAGCCGCGG GTTGCTCGTT CAGCTCGACG AGGCGTTCGT GCGGGCCGAG GCCGATGACG CCGTTCGGGT GGTCATCCTG GCTGCGCGCG GCAAAAACTT CTCCGCCGGT CACGACCTGG GCTCGGAGCT GGCGCTCGCC GAACGCCAGC CCGGGCCGGG CCAGCATCCG ACCTTCCGGT TCAACGGCGC CACCCGCGAT CCTATCGCGG AGAAGACCTA CCTGCAGGAG TGGCACTACT TCTTCCAAAA CACTTGCCGC TGGCGCGATC TGCGCAAGAT CACCATCGCC CAGGTGCAGG GCAACGCGAT CTCGGCCGGC CTGATGCTGA TCTGGGCGTG CGATCTGATC GTGGCCGCCG ACAACGCCAA GTTCAGCGAT GTGGTCGGCG TCCGGATGGG CATGCCGGGC GTCGAATACT ACGCGCACCC TTGGGAATTC GGCCCCCGCA AGGCCAAAGA GCTGCTGCTG ACCGGTGATG CGCTGGACGC CGACGAGGCC TACCGGCTGG GCATGGTGTC CAAGGTGTTC CCGGCCGACG AATTGGCCGA CAAGACACTG GAATTCGCGC GCCGCATCGC CGAGCGGCCG ACTATGGCCG CCCTGCTGAT CAAGGACTCG GTCAACGCCG CCTCGGACGC GATGGGCTTC ACGGAGGCGC TGCGGCACGC GTTCCACATC CACGAGCTGG GTCACGCGCA CTGGGCGGCG CACAACGAGA ACCGCTATCC GGTCGGCCTG CCGCCCGACG TCGAGGACTG GCGCAACGCC AAGCCGACCA GGCTGGCCCG CCGCGACACC CCG
Details for MyavA.00358.b.A1.GE28519
HARVESTED ON: 2/9/2010
SEQUENCED ON: 2/22/2010
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTLSEQAGA QPGARPAEEL VTYETLDEGR IARIWLNRPD AHNAQSRGLL VQLDEAFVRA EADDAVRVVI LAARGKNFSA GHDLGSELAL AERQPGPGQH PTFRFNGATR DPIAEKTYLQ EWHYFFQNTC RWRDLRKITI AQVQGNAISA GLMLIWACDL IVAADNAKFS DVVGVRMGMP GVEYYAHPWE FGPRKAKELL LTGDALDADE AYRLGMVSKV FPADELADKT LEFARRIAER PTMAALLIKD SVNAASDAMG FTEALRHAFH IHELGHAHWA AHNENRYPVG LPPDVEDWRN AKPTRLARRD TP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgactc tctccgaaca agcgggggcg cagcccggcg cccggccggc cgaggagctg gtgacctacg aaaccctcga cgagggccgc atcgcgcgca tctggctcaa ccggcccgac gcccacaacg cgcagagccg cgggttgctc gttcagctcg acgaggcgtt cgtgcgggcc gaggccgatg acgccgttcg ggtggtcatc ctggctgcgc gcggcaaaaa cttctccgcc ggtcacgacc tgggctcgga gctggcgctc gccgaacgcc agcccgggcc gggccagcat ccgaccttcc ggttcaacgg cgccacccgc gatcctatcg cggagaagac ctacctgcag gagtggcact acttcttcca aaacacttgc cgctggcgcg atctgcgcaa gatcaccatc gcccaggtgc agggcaacgc gatctcggcc ggcctgatgc tgatctgggc gtgcgatctg atcgtggccg ccgacaacgc caagttcagc gatgtggtcg gcgtccggat gggcatgccg ggcgtcgaat actacgcgca cccttgggaa ttcggccccc gcaaggccaa agagctgctg ctgaccggtg atgcgctgga cgccgacgag gcctaccggc tgggcatggt gtccaaggtg ttcccggccg acgaattggc cgacaagaca ctggaattcg cgcgccgcat cgccgagcgg ccgactatgg ccgccctgct gatcaaggac tcggtcaacg ccgcctcgga cgcgatgggc ttcacggagg cgctgcggca cgcgttccac atccacgagc tgggtcacgc gcactgggcg gcgcacaacg agaaccgcta tccggtcggc ctgccgcccg acgtcgagga ctggcgcaac gccaagccga ccaggctggc ccgccgcgac accccgtaat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTLSEQAGA QPGARPAEEL VTYETLDEGR IARIWLNRPD AHNAQSRGLL VQLDEAFVRA EADDAVRVVI LAARGKNFSA GHDLGSELAL AERQPGPGQH PTFRFNGATR DPIAEKTYLQ EWHYFFQNTC RWRDLRKITI AQVQGNAISA GLMLIWACDL IVAADNAKFS DVVGVRMGMP GVEYYAHPWE FGPRKAKELL LTGDALDADE AYRLGMVSKV FPADELADKT LEFARRIAER PTMAALLIKD SVNAASDAMG FTEALRHAFH IHELGHAHWA AHNENRYPVG LPPDVEDWRN AKPTRLARRD TP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactctctcc gaacaagcgg gggcgcagcc cggcgcccgg ccggccgagg agctggtgac ctacgaaacc ctcgacgagg gccgcatcgc gcgcatctgg ctcaaccggc ccgacgccca caacgcgcag agccgcgggt tgctcgttca gctcgacgag gcgttcgtgc gggccgaggc cgatgacgcc gttcgggtgg tcatcctggc tgcgcgcggc aaaaacttct ccgccggtca cgacctgggc tcggagctgg cgctcgccga acgccagccc gggccgggcc agcatccgac cttccggttc aacggcgcca cccgcgatcc tatcgcggag aagacctacc tgcaggagtg gcactacttc ttccaaaaca cttgccgctg gcgcgatctg cgcaagatca ccatcgccca ggtgcagggc aacgcgatct cggccggcct gatgctgatc tgggcgtgcg atctgatcgt ggccgccgac aacgccaagt tcagcgatgt ggtcggcgtc cggatgggca tgccgggcgt cgaatactac gcgcaccctt gggaattcgg cccccgcaag gccaaagagc tgctgctgac cggtgatgcg ctggacgccg acgaggccta ccggctgggc atggtgtcca aggtgttccc ggccgacgaa ttggccgaca agacactgga attcgcgcgc cgcatcgccg agcggccgac tatggccgcc ctgctgatca aggactcggt caacgccgcc tcggacgcga tgggcttcac ggaggcgctg cggcacgcgt tccacatcca cgagctgggt cacgcgcact gggcggcgca caacgagaac cgctatccgg tcggcctgcc gcccgacgtc gaggactggc gcaacgccaa gccgaccagg ctggcccgcc gcgacacccc gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa