MyavA.00358.a

Enoyl-CoA hydratase

CENTER ID: MyavA.00358.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00358.a.A1.GE28518 Full length( MyavA.00358.a ) 1 251
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00358.a.A1.PS00575 Full length( MyavA.00358.a ) 1 251

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.591
RefSeq: YP_879890.1
UniProt: A0A0H2ZUH0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPITSKTIEP GIVAVTVDFP PVNAIPSRGW FDLADTVTAA GQDPETHVVI LRAEGRGFNA GVDIKEMQRT EGFTALIDAN RGCFAAFRAV YECAVPVVAA VNGFCVGGGI GLVGNSDVIV ASDDATFGLP EVERGALGAA THLSRLVPQH LMRRLFFTAA TVDAATLHHF GSVHEVVPRG ELDDAALRVA REIAAKDTRV IRAAKEALNF IDVQRVNSSY RMEQGFTFEL NLAGVSDEHR DAFAGTQKGK K
NT Sequence
TTGCCCATCA CATCCAAAAC CATCGAACCG GGCATCGTCG CGGTCACCGT CGACTTCCCG CCCGTCAACG CCATCCCGTC GCGCGGCTGG TTCGACCTCG CCGACACCGT CACCGCGGCC GGCCAGGACC CCGAGACCCA CGTGGTGATC CTGCGGGCCG AGGGCCGCGG CTTCAACGCC GGGGTGGACA TCAAGGAGAT GCAGCGCACC GAGGGATTCA CCGCGCTGAT CGACGCCAAC CGCGGCTGCT TCGCCGCGTT CCGGGCGGTC TACGAGTGCG CGGTGCCCGT CGTCGCGGCG GTCAACGGAT TCTGCGTCGG CGGCGGCATC GGCCTGGTCG GCAACTCCGA TGTCATCGTC GCCTCCGACG ACGCCACCTT CGGGCTGCCC GAGGTGGAGC GGGGCGCGCT CGGCGCCGCC ACCCACCTGT CCCGGCTGGT GCCACAGCAC CTGATGCGCA GGCTGTTCTT CACCGCCGCC ACCGTCGACG CCGCCACCCT GCACCACTTC GGGTCGGTGC ACGAGGTGGT GCCGCGCGGC GAGCTGGATG ACGCGGCGCT GCGGGTCGCG CGCGAGATCG CCGCCAAGGA CACCCGGGTC ATCCGGGCGG CCAAGGAGGC GCTGAACTTC ATCGACGTGC AGCGGGTCAA CTCGAGTTAC CGGATGGAGC AAGGCTTTAC GTTCGAGCTC AATCTGGCCG GGGTGTCCGA CGAGCACCGC GACGCATTCG CCGGCACCCA GAAGGGCAAG AAA
Details for MyavA.00358.a.A1.GE28518
HARVESTED ON: 2/9/2010
SEQUENCED ON: 2/22/2010
EXPECTED MW: 29kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLPITSKTI EPGIVAVTVD FPPVNAIPSR GWFDLADTVT AAGQDPETHV VILRAEGRGF NAGVDIKEMQ RTEGFTALID ANRGCFAAFR AVYECAVPVV AAVNGFCVGG GIGLVGNSDV IVASDDATFG LPEVERGALG AATHLSRLVP QHLMRRLFFT AATVDAATLH HFGSVHEVVP RGELDDAALR VAREIAAKDT RVIRAAKEAL NFIDVQRVNS SYRMEQGFTF ELNLAGVSDE HRDAFAGTQK G
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgc ccatcacatc caaaaccatc gaaccgggca tcgtcgcggt caccgtcgac ttcccgcccg tcaacgccat cccgtcgcgc ggctggttcg acctcgccga caccgtcacc gcggccggcc aggaccccga gacccacgtg gtgatcctgc gggccgaggg ccgcggcttc aacgccgggg tggacatcaa ggagatgcag cgcaccgagg gattcaccgc gctgatcgac gccaaccgcg gctgcttcgc cgcgttccgg gcggtctacg agtgcgcggt gcccgtcgtc gcggcggtca acggattctg cgtcggcggc ggcatcggcc tggtcggcaa ctccgatgtc atcgtcgcct ccgacgacgc caccttcggg ctgcccgagg tggagcgggg cgcgctcggc gccgccaccc acctgtcccg gctggtgcca cagcacctga tgcgcaggct gttcttcacc gccgccaccg tcgacgccgc caccctgcac cacttcgggt cggtgcacga ggtggtgccg cgcggcgagc tggatgacgc ggcgctgcgg gtcgcgcgcg agatcgccgc caaggacacc cgggtcatcc gggcggccaa ggaggcgctg aacttcatcg acgtgcagcg ggtcaactcg agttaccgga tggagcaagg ctttacgttc gagctcaatc tggccggggt gtccgacgag caccgcgacg cattcgccgg cacccagaag ggca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLPITSKTI EPGIVAVTVD FPPVNAIPSR GWFDLADTVT AAGQDPETHV VILRAEGRGF NAGVDIKEMQ RTEGFTALID ANRGCFAAFR AVYECAVPVV AAVNGFCVGG GIGLVGNSDV IVASDDATFG LPEVERGALG AATHLSRLVP QHLMRRLFFT AATVDAATLH HFGSVHEVVP RGELDDAALR VAREIAAKDT RVIRAAKEAL NFIDVQRVNS SYRMEQGFTF ELNLAGVSDE HRDAFAGTQK GKK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcccatc acatccaaaa ccatcgaacc gggcatcgtc gcggtcaccg tcgacttccc gcccgtcaac gccatcccgt cgcgcggctg gttcgacctc gccgacaccg tcaccgcggc cggccaggac cccgagaccc acgtggtgat cctgcgggcc gagggccgcg gcttcaacgc cggggtggac atcaaggaga tgcagcgcac cgagggattc accgcgctga tcgacgccaa ccgcggctgc ttcgccgcgt tccgggcggt ctacgagtgc gcggtgcccg tcgtcgcggc ggtcaacgga ttctgcgtcg gcggcggcat cggcctggtc ggcaactccg atgtcatcgt cgcctccgac gacgccacct tcgggctgcc cgaggtggag cggggcgcgc tcggcgccgc cacccacctg tcccggctgg tgccacagca cctgatgcgc aggctgttct tcaccgccgc caccgtcgac gccgccaccc tgcaccactt cgggtcggtg cacgaggtgg tgccgcgcgg cgagctggat gacgcggcgc tgcgggtcgc gcgcgagatc gccgccaagg acacccgggt catccgggcg gccaaggagg cgctgaactt catcgacgtg cagcgggtca actcgagtta ccggatggag caaggcttta cgttcgagct caatctggcc ggggtgtccg acgagcaccg cgacgcattc gccggcaccc agaagggcaa gaaaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MyavA.00358.a.A1.PS00575
PURIFICATION DATe: 4/23/2010
CONCENTRATION: 13.02mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLPITSKTI EPGIVAVTVD FPPVNAIPSR GWFDLADTVT AAGQDPETHV VILRAEGRGF NAGVDIKEMQ RTEGFTALID ANRGCFAAFR AVYECAVPVV AAVNGFCVGG GIGLVGNSDV IVASDDATFG LPEVERGALG AATHLSRLVP QHLMRRLFFT AATVDAATLH HFGSVHEVVP RGELDDAALR VAREIAAKDT RVIRAAKEAL NFIDVQRVNS SYRMEQGFTF ELNLAGVSDE HRDAFAGTQK G
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgc ccatcacatc caaaaccatc gaaccgggca tcgtcgcggt caccgtcgac ttcccgcccg tcaacgccat cccgtcgcgc ggctggttcg acctcgccga caccgtcacc gcggccggcc aggaccccga gacccacgtg gtgatcctgc gggccgaggg ccgcggcttc aacgccgggg tggacatcaa ggagatgcag cgcaccgagg gattcaccgc gctgatcgac gccaaccgcg gctgcttcgc cgcgttccgg gcggtctacg agtgcgcggt gcccgtcgtc gcggcggtca acggattctg cgtcggcggc ggcatcggcc tggtcggcaa ctccgatgtc atcgtcgcct ccgacgacgc caccttcggg ctgcccgagg tggagcgggg cgcgctcggc gccgccaccc acctgtcccg gctggtgcca cagcacctga tgcgcaggct gttcttcacc gccgccaccg tcgacgccgc caccctgcac cacttcgggt cggtgcacga ggtggtgccg cgcggcgagc tggatgacgc ggcgctgcgg gtcgcgcgcg agatcgccgc caaggacacc cgggtcatcc gggcggccaa ggaggcgctg aacttcatcg acgtgcagcg ggtcaactcg agttaccgga tggagcaagg ctttacgttc gagctcaatc tggccggggt gtccgacgag caccgcgacg cattcgccgg cacccagaag ggca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLPITSKTI EPGIVAVTVD FPPVNAIPSR GWFDLADTVT AAGQDPETHV VILRAEGRGF NAGVDIKEMQ RTEGFTALID ANRGCFAAFR AVYECAVPVV AAVNGFCVGG GIGLVGNSDV IVASDDATFG LPEVERGALG AATHLSRLVP QHLMRRLFFT AATVDAATLH HFGSVHEVVP RGELDDAALR VAREIAAKDT RVIRAAKEAL NFIDVQRVNS SYRMEQGFTF ELNLAGVSDE HRDAFAGTQK GKK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGCCCATC ACATCCAAAA CCATCGAACC GGGCATCGTC GCGGTCACCG TCGACTTCCC GCCCGTCAAC GCCATCCCGT CGCGCGGCTG GTTCGACCTC GCCGACACCG TCACCGCGGC CGGCCAGGAC CCCGAGACCC ACGTGGTGAT CCTGCGGGCC GAGGGCCGCG GCTTCAACGC CGGGGTGGAC ATCAAGGAGA TGCAGCGCAC CGAGGGATTC ACCGCGCTGA TCGACGCCAA CCGCGGCTGC TTCGCCGCGT TCCGGGCGGT CTACGAGTGC GCGGTGCCCG TCGTCGCGGC GGTCAACGGA TTCTGCGTCG GCGGCGGCAT CGGCCTGGTC GGCAACTCCG ATGTCATCGT CGCCTCCGAC GACGCCACCT TCGGGCTGCC CGAGGTGGAG CGGGGCGCGC TCGGCGCCGC CACCCACCTG TCCCGGCTGG TGCCACAGCA CCTGATGCGC AGGCTGTTCT TCACCGCCGC CACCGTCGAC GCCGCCACCC TGCACCACTT CGGGTCGGTG CACGAGGTGG TGCCGCGCGG CGAGCTGGAT GACGCGGCGC TGCGGGTCGC GCGCGAGATC GCCGCCAAGG ACACCCGGGT CATCCGGGCG GCCAAGGAGG CGCTGAACTT CATCGACGTG CAGCGGGTCA ACTCGAGTTA CCGGATGGAG CAAGGCTTTA CGTTCGAGCT CAATCTGGCC GGGGTGTCCG ACGAGCACCG CGACGCATTC GCCGGCACCC AGAAGGGCAA GAAAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA