SSGCID: Target Page

MyavA.00337.b

Dehydrogenase

CENTER ID:	MyavA.00337.b
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.00337.b.A1.GE28516	Full length( MyavA.00337.b )		1	296

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.00337.b.A1.PS00670	Full length( MyavA.00337.b )		1	296

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.3744
RefSeq:	YP_882994.1
UniProt:	A0A0H2ZUK5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTIPWTPDRL GDLTGKRVIV TGATNGVGLG TARALAKAGA EVILAVRNTE LGKQRAAQMG GSTAVEKLDL ADLSSVRAFA DRIEAPVDIL INNAGALTDR RTETVDGFEM TLGTNLLGPF ALTNLLLPKV RSQIINVGSD AHRSATLHLD DLHLRRHKWT RLGAYAQSKL AVMLWGLELD RRLRAAGSPI VTQLTHPGWV ASNLSNLGDA PLKAAAHKAV KMVADRLAND IDEGAAPTLY CISEPIPPGS YVGVSGRFGL RGGPVLIGRT PLACDYDTAA RLVAFAERET GTELRV

NT Sequence

GTGACCATAC CGTGGACCCC GGACCGGCTG GGCGACCTCA CCGGCAAACG CGTGATCGTG ACAGGAGCCA CCAACGGCGT CGGGCTGGGC ACCGCCCGTG CCCTGGCCAA GGCCGGCGCC GAGGTGATCC TGGCCGTGCG CAACACCGAA CTCGGGAAGC AACGCGCGGC CCAGATGGGC GGGTCGACCG CCGTGGAAAA ACTCGACCTG GCCGACCTGT CGTCGGTGCG CGCCTTCGCC GACCGGATCG AGGCGCCGGT GGACATCCTG ATCAACAACG CCGGCGCCCT GACCGACCGG CGCACCGAGA CGGTCGACGG CTTCGAGATG ACGCTGGGCA CCAACCTGCT CGGGCCGTTC GCGCTGACCA ACCTGCTGCT GCCCAAGGTG CGCAGCCAGA TCATCAACGT CGGCTCCGAC GCGCACCGCT CGGCGACGCT GCACCTCGAC GACCTGCATC TGCGCCGGCA CAAGTGGACC CGGCTGGGCG CCTACGCGCA GTCCAAGCTC GCGGTCATGC TGTGGGGGCT CGAGCTGGAC CGCCGGCTGC GCGCGGCGGG GTCGCCGATC GTCACCCAGC TGACCCACCC GGGTTGGGTG GCGTCGAACC TGTCCAACCT GGGCGACGCG CCGCTGAAGG CGGCGGCGCA CAAGGCGGTC AAAATGGTGG CCGACCGGCT GGCCAACGAC ATCGACGAGG GCGCGGCGCC CACCCTGTAC TGCATCAGCG AGCCGATCCC GCCCGGCAGC TACGTCGGGG TCAGCGGCAG GTTCGGGCTG CGCGGCGGCC CGGTGCTGAT CGGGCGCACC CCGCTGGCCT GCGATTACGA CACGGCCGCG CGACTGGTGG CCTTCGCCGA GCGCGAGACC GGCACGGAAC TGAGGGTC

Details for MyavA.00337.b.A1.GE28516

HARVESTED ON:	2/9/2010
SEQUENCED ON:	2/22/2010
EXPECTED MW:	33kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVTIPWTPD RLGDLTGKRV IVTGATNGVG LGTARALAKA GAEVILAVRN TELGKQRAAQ MGGSTAVEKL DLADLSSVRA FADRIEAPVD ILINNAGALT DRRTETVDGF EMTLGTNLLG PFALTNLLLP KVRSQIINVG SDAHRSATLH LDDLHLRRHK WTRLGAYAQS KLAVMLWGLE LDRRLRAAGS PIVTQLTHPG WVASNLSNLG DAPLKAAAHK AVKMVADRLA NDIDEGAAPT LYCISEPIPP GSYVGVSGRF GLRGGPVLIG RTPLACDYDT AARLVAFAER ETGTELRV

Validated NT Sequence

tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgac cataccgtgg accccggacc ggctgggcga cctcaccggc aaacgcgtga tcgtgacagg agccaccaac ggcgtcgggc tgggcaccgc ccgtgccctg gccaaggccg gcgccgaggt gatcctggcc gtgcgcaaca ccgaactcgg gaagcaacgc gcggcccaga tgggcgggtc gaccgccgtg gaaaaactcg acctggccga cctgtcgtcg gtgcgcgcct tcgccgaccg gatcgaggcg ccggtggaca tcctgatcaa caacgccggc gccctgaccg accggcgcac cgagacggtc gacggcttcg agatgacgct gggcaccaac ctgctcgggc cgttcgcgct gaccaacctg ctgctgccca aggtgcgcag ccagatcatc aacgtcggct ccgacgcgca ccgctcggcg acgctgcacc tcgacgacct gcatctgcgc cggcacaagt ggacccggct gggcgcctac gcgcagtcca agctcgcggt catgctgtgg gggctcgagc tggaccgccg gctgcgcgcg gcggggtcgc cgatcgtcac ccagctgacc cacccgggtt gggtggcgtc gaacctgtcc aacctgggcg acgcgccgct gaaggcggcg gcgcacaagg cggtcaaaat ggtggccgac cggctggcca acgacatcga cgagggcgcg gcgcccaccc tgtactgcat cagcgagccg atcccgcccg gcagctacgt cggggtcagc ggcaggttcg ggctgcgcgg cggcccggtg ctgatcgggc gcaccccgct ggcctgcgat tacgacacgg ccgcgcgact ggtggccttc gccgagcgcg agaccggcac ggaactgagg gtctaataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccata ccgtggaccc cggaccggct gggcgacctc accggcaaac gcgtgatcgt gacaggagcc accaacggcg tcgggctggg caccgcccgt gccctggcca aggccggcgc cgaggtgatc ctggccgtgc gcaacaccga actcgggaag caacgcgcgg cccagatggg cgggtcgacc gccgtggaaa aactcgacct ggccgacctg tcgtcggtgc gcgccttcgc cgaccggatc gaggcgccgg tggacatcct gatcaacaac gccggcgccc tgaccgaccg gcgcaccgag acggtcgacg gcttcgagat gacgctgggc accaacctgc tcgggccgtt cgcgctgacc aacctgctgc tgcccaaggt gcgcagccag atcatcaacg tcggctccga cgcgcaccgc tcggcgacgc tgcacctcga cgacctgcat ctgcgccggc acaagtggac ccggctgggc gcctacgcgc agtccaagct cgcggtcatg ctgtgggggc tcgagctgga ccgccggctg cgcgcggcgg ggtcgccgat cgtcacccag ctgacccacc cgggttgggt ggcgtcgaac ctgtccaacc tgggcgacgc gccgctgaag gcggcggcgc acaaggcggt caaaatggtg gccgaccggc tggccaacga catcgacgag ggcgcggcgc ccaccctgta ctgcatcagc gagccgatcc cgcccggcag ctacgtcggg gtcagcggca ggttcgggct gcgcggcggc ccggtgctga tcgggcgcac cccgctggcc tgcgattacg acacggccgc gcgactggtg gccttcgccg agcgcgagac cggcacggaa ctgagggtca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for MyavA.00337.b.A1.PS00670

PURIFICATION DATe:	7/8/2010
CONCENTRATION:	21.45mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	13
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACCATA CCGTGGACCC CGGACCGGCT GGGCGACCTC ACCGGCAAAC GCGTGATCGT GACAGGAGCC ACCAACGGCG TCGGGCTGGG CACCGCCCGT GCCCTGGCCA AGGCCGGCGC CGAGGTGATC CTGGCCGTGC GCAACACCGA ACTCGGGAAG CAACGCGCGG CCCAGATGGG CGGGTCGACC GCCGTGGAAA AACTCGACCT GGCCGACCTG TCGTCGGTGC GCGCCTTCGC CGACCGGATC GAGGCGCCGG TGGACATCCT GATCAACAAC GCCGGCGCCC TGACCGACCG GCGCACCGAG ACGGTCGACG GCTTCGAGAT GACGCTGGGC ACCAACCTGC TCGGGCCGTT CGCGCTGACC AACCTGCTGC TGCCCAAGGT GCGCAGCCAG ATCATCAACG TCGGCTCCGA CGCGCACCGC TCGGCGACGC TGCACCTCGA CGACCTGCAT CTGCGCCGGC ACAAGTGGAC CCGGCTGGGC GCCTACGCGC AGTCCAAGCT CGCGGTCATG CTGTGGGGGC TCGAGCTGGA CCGCCGGCTG CGCGCGGCGG GGTCGCCGAT CGTCACCCAG CTGACCCACC CGGGTTGGGT GGCGTCGAAC CTGTCCAACC TGGGCGACGC GCCGCTGAAG GCGGCGGCGC ACAAGGCGGT CAAAATGGTG GCCGACCGGC TGGCCAACGA CATCGACGAG GGCGCGGCGC CCACCCTGTA CTGCATCAGC GAGCCGATCC CGCCCGGCAG CTACGTCGGG GTCAGCGGCA GGTTCGGGCT GCGCGGCGGC CCGGTGCTGA TCGGGCGCAC CCCGCTGGCC TGCGATTACG ACACGGCCGC GCGACTGGTG GCCTTCGCCG AGCGCGAGAC CGGCACGGAA CTGAGGGTCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A